Kareius bicoloratus (Basilewsky) is one of the commonly found right-eye flounders and widely distributed in the coastal waters of Korea and Japan. On December 11,1980, the ailthors carried out an experiment to obtain a large number of fertilized eggs from wild adult fish caught by a trawler. The fish were obtained from Maisaka fish market, Shizuoka Prefecture, Japan. The egg is pelagic, spherical in shape and measuring 1.014-1.04 mm in diameter. The yolk as well as the egg capsule is colorless and transparent, and contain no oil globules. The hatching took place in 73 hr 45 min after fertilization at the water temperature $8.0-9.5^{\circ}C$. Newly hatched larvae are 3.09-3.146 mm in total length, with the anus situated in the middle of the body. The marginal fin does not have Pigment cells and myotome number is 17+20=37. Within one day after hatching, the larvae attained 3.77 mm in total length, and there appeared three or four melanophore on yolk sac. When the larvae attained 3.96 mm in total length, melanophores began to appear on the eye ball. Two days after hatching, the larvae attained 4.05 mm in total length, most of yolk material was absorbed, and the caudal fin began to grow at the terminal part of the notochord. When the larvae attained 4.21 mm in total length, mouth and eyes began to move. After 3 days, the larvae attained 4.342-4.394 mm in total length, alimentary canal differentiated, melanophores appeared on the lower jaw and posterior part of the fin membrane. When the larvae attaind 4.576 mm in total length, marginal line of dorsal fin membrane became concave.
Kim, Hyeong-Seob;Kim, Eung-Jo;Lee, Joo-Ho;Ji, Seong-Hak
Korean Journal of Biological Psychiatry
/
v.11
no.2
/
pp.117-126
/
2004
Objects:It has been reported that the incidence of tardive dyskinesia(TD), the remarkable abnormal involuntary movement, was higher in the schizophrenics with high blood sugar levels and that TD had been improved by small amount of insulin-injection for 90 days. And also it was generally known that the blood lipids were higher in the schizophrenics with tardive dyskinesia. Thus, we tried to replicate the correlations of abnormal involuntary movements with blood sugar levels and blood lipids in chronic schizophrenics treated with antipsychotics. Methods:Thirty-eight male schizophrenic inpatients who were stable in clinical state with medications, were included. The patients who had been already diagnosed as diabetes mellitus(DM), organic brain disorder, substance- related disorder, physical illness were excluded and also we excluded female patients to remove the hormonal effect on TD. Eleven patients who ranked higher(above five) in the Abnormal Involuntary Movement Scale(AIMS) were assigned into 2 groups, a dibenese group and a placebo group. Diabinese or placebos were administrated for 3 weeks with antipsychotics and AIMS was rechecked. Results:There were no correlations between the total AIMS scores and blood sugar and lipids levels in all subjects. The means of total and subscale scores(objective, face, and extremity) of AIMS did not reveal statistical significances between diabinese and placebo groups. However(total, jaw, face, upper arm, and objective feeling), were statistically higher in the diabinese group than those in the placebo group. And correlations of total cholesterol(TC) with fast blood sugar(FBS), weight with body mass index(BMI) and waist, total glycerol (TG) with BMI were statistically significant. Conclusion:In this study, there were statistical significances in the changes in ratings of AIMS scores between the diabinese group and the placebo group. Application of oral hypoglycemic agent might be a way of improving abnormal involuntary movements in schizophrenics with abnormal involuntary movements or TD. Althogugh it was not certain that there were correlations of abnormal involuntary movement with blood sugar and lipids, correlations of TC/TG with AIMS, of FBS with AIMS cautiously suggest that the regular check of $HbA_1C$, waist, and weight are recommended for schizophrenics.
The influence of starvation on morphological change of the red sea bream larvae was examined at Song-ji fish hatchery, Tongyong-Gun, Kyongnam Provice in July 1988. The results obtained are as follows: 1) The larvae of red sea bream began to feed on rotifers in 2 days after hatching. In case of non-feeding, all of the larvae died in 5 days after hatching and the larvae which feeding delayed 1 and 2 days from normal first feeding schedule also died 100 in 6 days after hatching. 2) With the exhaustion of the yolk, the total length, body length, myotome height and gut height of unfed larvae decreased. 3) The ratio of height to myotome height in unfed larvae has declined most rapidly compare to other demensions while starving. At 5 days after hatching, the ratios of these of starving larvae and fed larvae were 0.306 and 0.010, respectively. 4) The morphology of starving larvae at 6 days after hatching are characterized as sharpened jaw, projected edge of lower part of clavicle and slender gut.
The purpose of the present study was to describe the morphological and skeletal development of the larvae and juvenile of Ilisha elongata(Bennett). The larvae(mean 5.02 mm in total length, TL) opened the mouth and anus with 36~38+15~16=51~54 myotomes, and anus located about 73.0% of TL. The clavicle of shoulder girdle was ossified. The larvae(mean 7.82 mm in TL) have all fin-fold differentiated and its caudal notochord began to flex. Parasphenoid of cranium and dentary of the lower jaw were ossified. The caudal notochord of the larva(mean 9.84 mm) was flexed $45^{\circ}$, and anus located about 67.0% of TL. The body height of the larvae(mean 23.32 mm in TL) were 18.0~20.0% of TL, and the aggregate numbers of all fin rays were completed. Ossification was completed in the larva of mean 26.52 mm in TL.
The horn fish, Hemiramphus sajori TEMMINCK et SCHLEGEL, is widely distributed in the coastal waters of Korea, Japan and Taiwan. On April 13, 1983, mature adults of the horn fish were collected from Changson channel, Namhae. The authors carried out artificial insemination on the field, and incubated the eggs and reared larvae in the laboratory. And these larvae were reared for about two weeks in glass jars, fed brine shrimp nauplii. The eggs of this species are demersal and adhesive, with 4 to 6 filaments at the animal pole and a filament at the vegetal pole. The diameter of these eggs varies 1.80 to 2.00 mm. The hatching took place in ca. 376 hours after fertilization. During the incubation period water temperature fluctuated between $13.5^{\circ}C\;and\;20.0^{\circ}C$. The newly hatched larvae were elongate in shape $7.40{\sim}8.00$ mm in total length with $40+16{\sim}17=56{\sim}57$ myomers. Many melanophore were distributed on the head, both sides of dorsal body contour, peritoneum and ventral line of the tail part. Four days after hatching, the larvae were from 9.00 to 9.65 mm in total length, at this tims the larvae absorbed the yolk completely, and became postlarvae. Fourteen days after hatching, the larvae attained 13.25 mm in total length, with 14 rays of the dorsal fin, 13 rays of the anal fin, and 4 rays of pectoral fin. The lower jaw has somewhat projected.
Journal of the Korean Association of Oral and Maxillofacial Surgeons
/
v.40
no.1
/
pp.21-26
/
2014
Objectives: The purpose of this article is to analyze the incidence, demographic distribution, type, and etiology of mandible fractures that were treated by the Department of Oral and Maxillofacial Surgery in Kyung Hee University Dental Hospital from January 2002 to December 2012. Materials and Methods: This was a descriptive and analytic retrospective study that evaluated 735 patients that were treated for mandible fracture. Results: This study included 1,172 fractures in 735 patients. The ratio of male to female patients was 5.45 : 1; the maximum value was in patients between 20 and 29 years (38.1%) and the minimum in patients over 70 years old. The monthly distribution of facial fractures peaked in the fall and was lower during winter. No specific correlation was identified based on the annual fracture distribution. Among the 735 fracture patients, 1.59 fracture lines were observed per patient. The most frequent site was the symphysis, which accounted for a total of 431 fractures, followed by the angle (348), condyle (279), and body (95). The symphysis with angle was the most common site identified in combination with fracture and accounted for 22.4%, followed by symphysis with condyle (19.8%). The angle was the most frequent site of single fractures (20.8%). The major cause of injury was accidental trauma (43.4%), which was followed by other causes such as violence (33.9%), sports-related accidents (10.5%), and traffic accidents (10.1%). Fracture incidents correlated with alcohol consumption were reported between 10.0%-26.9% annually. Conclusion: Although mandible fracture pattern is similar to the previous researches, there is some changes in the etiologic factors.
Journal of the korean academy of Pediatric Dentistry
/
v.38
no.1
/
pp.95-100
/
2011
Fibrodysplasia ossificans progressiva(FOP) is characterized by episodes of permanent heterotopic ossifications of soft tissues throughout the body. FOP is inherited in an autosomal dominant pattern, but most cases result from new mutations in the ACVR1 gene. Even minimal trauma can cause permanent ossifications of soft tissues and give rise to complications following routine dental care. Dental block anesthesia, severe stretching of the jaw and biopsies are all contraindicated in children with FOP. There is no effective treatment. Since the prevalence of FOP is very low and most patients with FOP are misdiagnosed during childhood, they undergo dangerous and unnecessary treatment that can lead to permanent harm. For patients with FOP, early diagnosis and prevention of complications are most important. This study aims at contemplating the characteristic features and consideration factors for the dental treatment of FOP patients in relation to the case of an eight years-and-one month old boy who was referred to Pediatric Dentistry due to prolonged retention of mandibular right and left deciduous central incisors after being diagnosed with sporadic FOP at Pediatric Orthopaedics, Seoul National University Hospital, and received dental treatment without the exacerbation of the FOP symptoms.
Park, Ju-Un;Kim, Byung-Ock;Park, Joo-Cheol;Jang, Hyun-Seon
Journal of Periodontal and Implant Science
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v.36
no.1
/
pp.27-37
/
2006
Although the main purpose of periodontal treatment to regenerate is the complete regeneration of periodontal tissue due to periodontal disease, most of the treatment cannot meet such purpose because healing by long epithelial junction. Therefore, diverse materials of resorbable and non-resorbable have been used to regenerate the periodontal tissue. Due to high risk of exposure and necessity of secondary surgical procedure when using non-resorbable membrane, guided tissue regeneration using the resorbable membrane has gain popularity, recently. However, present resorbable membrane has the disadvantage of not having sufficient time to regenerate date to the difference of resorption rate according to surgical site. Meanwhile, other than the structure stability and facile manipulation, acellular dermal matrix has been reported to be a possible scaffold for cellular proliferation due to rapid revascularization and favorable physical properties for cellular attachment and proliferation. The purpose of this study is to estimate the influence of acellular dermal matrix on periodontal ligament, cementum and alveolar bone when acellular dermal matrix is implanted to 1-wall alveolar bone defect. 4 dogs of 12 to 16 month old irrelevant to sex , which below 15Kg of body weight, has been used in this study. ADM has been used for the material of guided tissue regeneration. The 3rd premolar of the lower jaw was extracted bilaterally and awaited for self-healing. subsequently buccal and lingual flap was elevated to form one wall intrabony defect with the depth and width of 4mm on the distal surface of 2nd premolar and the mesial surface of 4th premolar. After the removal of periodontal ligament by root planing. notch was formed on the basal position. Following the root surface treatment, while the control group had the flap sutured without any treatment on surgically induced intrabony defect. Following the root surface treatment, the flap of intrabony defect was sutured with the ADM inserted while the control group sutured without any insertion. The histologic specimen was observed after 4 and 8 weeks of treatment. The control group was partially regenerated by periodontal ligament, new cementum and new alveolar bone. the level of regeneration is not reached on the previous formed notch. but, experimental group was fully regenerated by functionally oriented periodontal ligament fiber. new cementum and new alveolar bone. In conclusion, we think that ADM seems to be used by scaffold for periodontal ligament cells and the matrix is expected to use on guided tissue regeneration.
Kim, Hyun-Ki;Kim, Eui-Seoung;Choi, In-Bok;Kim, Jin;Lee, Seung-Jong
Restorative Dentistry and Endodontics
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v.28
no.5
/
pp.385-391
/
2003
The purpose of this study is to examine the viability of PDL cells in rat molars by using MTT assay and to verify the MTT assay through the histologic observation. Thirty of Sprague-Dawley white female rats of 4-weeks old with a body weight of about 100 grams were used. Groupings are as follows : Immediate Group : Positive control group(n=10)-after extraction immediately. Dried Group : Negative control group(n=10)-after drying for an hour under warm dry. $ViaSpan^{\circledR}$ Group : 1hour $ViaSpan^{\circledR}$ group(n=10)-after storing in $ViaSpan^{\circledR}{\;}at{\;}4^{\circ}C$ for 1hour. Ten teeth of each group were treated as same as above and replanted to the original socket of experimental animals. After two weeks of replantation. all the experimental animals were sacrificed. And after fixation, extracted maxillary jaw was dimineralized. After it was embedded in paraffin. serial section by $5\mu\textrm{m}$ was carried out and for construction of specimen, hematoxylin-eosin dye was used. The mean MTT measurement of immediate group(positive control) is 2.81 and the mean measurement of dried group(negative control) is 0.98 which is significantt differnt(P<0.05), The mean measurement of $ViaSpan^{\circledR}$ group is 2.65 and there is significant difference between dried group and $ViaSpan^{\circledR}$ group(P<0.05), However, there is no difference between immediate group and $ViaSpan^{\circledR}$ group. The average resorption points of immediate group is 3.03 points. In the dried group, average 6.44 points resorption and 2.68 points showed resorption in the $ViaSpan^{\circledR}$ group. Unlike with MTT assay, there was no significant difference between the immediate group and $ViaSpan^{\circledR}$ group. The usage of MTT assay as a viable cell marker may give us a better indication of the maintenance of periodontal ligament cell vitality.
Objectives : Osteoporosis is a systemic skeletal disease that decreases bone density and increases the risk of fractures. Bisphosphonates and SERMs are mainly used to treat osteoporosis, but, long-term use increases the risk of side effects such as jaw bone necrosis and breast cancer. Therefore, it is necessary to develop a therapeutic agent for a natural product with few side effects. Water extract of Lonicerae Japonicae Flos (wLF) was mainly found to have anti-cancer and anti-inflammatory effects. However, the effect of wLF on osteoporosis has not been elucidated. Therefore, this experiment investigated the effect of wLF on osteoclasts, osteoblasts and osteoporosis models. Methods : In order to study the effect of wLF on osteoporosis, the OVX-induced rat model was used for in vivo study. After 8 weeks, we measured body weight, uterine weight, liver weight, femur weight, bone density, trabecular area and tibia ash weight. To determine the effect of wLF on osteoclast differentiation, we measured the number of TRAP-positive cells and TRAP activity. To examine the effect of wLF on the expression of osteoblast-related genes, we measured the mRNA expression of alkaline phosphatase (ALP, Alpl) and osteocalcin (OCN, Bglap2). Results : In vivo experiment, wLF inhibited the reduction of femur weight, trabecular area, bone density and tibia ash weight. In vitro experiment, wLF had no significant effect on osteoclast differentiation. However, wLF increased the mRNA expression of Alpl and Bglap2 in MC3T3-E1 cell. Conclusions : This result suggested that wLF may be used for the treatment and prevention of postmenopausal osteoporosis.
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