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http://dx.doi.org/10.6116/kjh.2021.36.5.81.

Effect of Lonicerae Japonicae Flos on Bone Density in Ovariectomized Rat Model of Osteoporosis  

Lee, SungYub (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Minsun (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Hong, SooYeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Jae-Hyun (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Kim, Hongsik (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Lee, Chungho (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
Publication Information
The Korea Journal of Herbology / v.36, no.5, 2021 , pp. 81-91 More about this Journal
Abstract
Objectives : Osteoporosis is a systemic skeletal disease that decreases bone density and increases the risk of fractures. Bisphosphonates and SERMs are mainly used to treat osteoporosis, but, long-term use increases the risk of side effects such as jaw bone necrosis and breast cancer. Therefore, it is necessary to develop a therapeutic agent for a natural product with few side effects. Water extract of Lonicerae Japonicae Flos (wLF) was mainly found to have anti-cancer and anti-inflammatory effects. However, the effect of wLF on osteoporosis has not been elucidated. Therefore, this experiment investigated the effect of wLF on osteoclasts, osteoblasts and osteoporosis models. Methods : In order to study the effect of wLF on osteoporosis, the OVX-induced rat model was used for in vivo study. After 8 weeks, we measured body weight, uterine weight, liver weight, femur weight, bone density, trabecular area and tibia ash weight. To determine the effect of wLF on osteoclast differentiation, we measured the number of TRAP-positive cells and TRAP activity. To examine the effect of wLF on the expression of osteoblast-related genes, we measured the mRNA expression of alkaline phosphatase (ALP, Alpl) and osteocalcin (OCN, Bglap2). Results : In vivo experiment, wLF inhibited the reduction of femur weight, trabecular area, bone density and tibia ash weight. In vitro experiment, wLF had no significant effect on osteoclast differentiation. However, wLF increased the mRNA expression of Alpl and Bglap2 in MC3T3-E1 cell. Conclusions : This result suggested that wLF may be used for the treatment and prevention of postmenopausal osteoporosis.
Keywords
Water extract of Lonicerae Japonicae Flos; osteoporosis; osteoclast; osteoblast; ovariectomized;
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1 L. Ginaldi, M. C. Di Benedetto, and M. De Martinis, "Osteoporosis, inflammation and ageing," Immunity and Ageing. 2005 ; 2 : 14.   DOI
2 G. R. Mundy, "Osteoporosis and inflammation," Arq Bras Endocrinol Metabol. 2010; 54(2): 123-32.   DOI
3 凌一揆. 中藥 本草學. 서울:保健新聞社. 1998.
4 Han JS, Park HS. The Effects of Anti-cancer and Immune Response of Lonicerae Flos Herbal-acupuncture. J Acupunct Res. 2006 ; 23(4) : 205-218.
5 Ohta S, Sato N, Tu SH, Shinoda M. Yakugaku Zasshil. Protective effects of Taiwan crude drugs on experimental liver injuries. 1993 ; 113 : 870-880.
6 Tie K, Wang H, Wang X, Chen L. Measurement of Bone Mineral Density in the Tunnel Regions for Anterior Cruciate Ligament Reconstruction by Dual-Energy X-Ray Absorptiometry, Computed Tomography Scan, and the Immersion Technique Based on Archimedes' Principle. Arthroscopy. 2012 ; 28(10) : 1464-1471.   DOI
7 M. H. Tschop, J. R. Speakman, J. R. S. Arch et al., "A guide to analysis of mouse energy metabolism," Nature Methods. 2011; 9(1): 57-63.   DOI
8 Tie K, Wang H, Wang X, Chen L. Measurement of Bone Mineral Density in the Tunnel Regions for Anterior Cruciate Ligament Reconstruction by Dual-Energy X-Ray Absorptiometry, Computed Tomography Scan, and the Immersion Technique Based on Archimedes' Principle. Arthroscopy: Arthroscopy. 2012 ; 28(10) : 1464-1471.   DOI
9 Notomi T, Okimoto N, Okazaki Y, Nakamura T, Suzuki M. Tower climbing exercise started 3 months after ovariectomy recovers bone strength of the femur and lumbar vertebrae in aged osteopenic rats. J Bone Miner Res. 2003 ; 18 : 140-149.   DOI
10 Yamamoto M, Fisher JE, Gentile M, Seedor JG, Leu CT, Rodan SB, Rodan GA. The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats. Endocrinology. 1998 ; 139(3) : 1411-1419.   DOI
11 Cummings SR, Bates D, Black DM. Clinical use of bone densitometry: scientific review. JAMA. 2002 ; 288 : 1889-1897.   DOI
12 Yamamoto M, Fisher JE, Gentile M, Seedor JG, Leu CT, Rodan SB, Rodan GA. The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats. Endocrinology. 1998 ; 139(3) : 1411-1419.   DOI
13 Wu X, Xie CQ, Zhu QQ, Wang MY, Sun B, Huang YP, Shen C, An MF, Zhao YL, Wang XJ and Sheng J: Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro. Food Nutr Res. 2018 ; 62.
14 ISO 10993-5:2009 Biological Evaluation of Medical Devices. Part 5: Tests for In Vitro Cytotoxicity. International Organization for Standardization; Geneva, Switzerland: 2009.
15 Lopez-Garcia J, Lehocky M, Humpolicek P, Saha P. HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation. J Funct Biomater. 2014 ; 5(2) : 43-57   DOI
16 Maehata Y, Takamizawa S, Ozawa S, Kato Y, Sato S, Kubota E, Hata RI. Both direct and collagen-mediated signals are required for active vitamin D 3-elicited differentiation of human osteoblastic cells: Roles of osterix, an osteoblast-related transcription factor. Matrix biology. 2006 ; 25(1) : 47-58.   DOI
17 Lee NK, Sowa H, Hinoi E, Ferron M, Ahn JD, Confavreux C, Dacquin R, Mee PJ, McKee MD, Jung DY, Zhang Z, Kim JK, Mauvais-Jarvis F, Ducy P, Karsenty G. Endocrine Regulation of Energy Metabolism by Skeleton. Cell. 2007 ; 130(3) : 456-469.   DOI
18 Collin-Osdoby P and Osdoby P: RANKL-mediated osteoclast formation from murine RAW 264.7 cells. Methods in molecular biology 2012 ; 816: 187-202.   DOI
19 Joan S Lewis 1, V Craig Jordan, Selective estrogen receptor modulators (SERMs): mechanisms of anticarcinogenesis and drug resistance. Mutat Res. 2005; 591(1-2): 247-63.   DOI
20 Kazakia GJ, Majumdar S. New imaging technologies in the diagnosis of osteoporosis. Reviews in Endocrine and Metabolic Disorders. 2006 ; 7 : 67-74.   DOI
21 Wang L, Liu S, Zhao Y, Liu D, Liu Y, Chen C, Karray S, Jin Y. Osteoblast-induced osteoclast apoptosis by fas ligand/FAS pathway is required for maintenance of bone mass. Cell Death & Differentiation. 2015; 22(10) : 1654-1664.26   DOI
22 Quarles LD, Yohay DA, Lever LW, Caton R and Wenstrup RJ: Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research. 1992 ; 7: 683-692.   DOI
23 Jeong Y, Yang W, Ko H, Kim M. The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1 cells. Restorative dentistry & endodontics. 2014 ; 39(3) : 187-194.   DOI
24 Guo C, Yuan L, Wang JG, Wang F, Yang XK, Zhang FH, Song JL, Ma XY, Cheng Q, Song GH. Lipopolysaccharide (LPS) induces the apoptosis and inhibits osteoblast differentiation through JNK pathway in MC3T3-E1 cells. Inflammation. 2014 ; 37(2) : 621-631.   DOI
25 Plantalech L, Guillaumont M, Vergnaud P, Leclercq M, Delmas PD. Impairment of gamma carboxylation of circulating osteocalcin (bone gla protein) in elderly women. Journal of Bone and Mineral Research. 1991 ; 6(11) : 1211-1216.   DOI
26 Chen X, Wang Z, Duan N, Zhu G, Schwarz EM, Xie C. Osteoblast-osteoclast interactions. Connective Tissue Research. 2018 ; 2017 : 1-9.
27 Consensus A. Consensus development conference: diagnosis, prophylaxis, and treatment of osteoporosis. Am J Med. 1993 ; 94(6) : 646-50.   DOI
28 I Yamazaki., H Yamaguchi. Characteristics of an ovariectomized osteopenic rat model. J Bone Miner Res. 1989 ; 4(1) : 13-22.   DOI
29 Yamamoto M, Fisher JE, Gentile M, Seedor JG, Leu CT, Rodan SB, Rodan GA. The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats. Endocrinology. 1998 ; 139(3) : 1411-1419.   DOI
30 Kuo TR and Chen CH: Bone biomarker for the clinical assessment of osteoporosis: Recent developments and future perspectives Biomark Res. 2017 ; 5: 18.   DOI
31 Sozen T, Ozisik L, Basaran NC. An overview and management of osteoporosis. Eur J Rheumatol. 2017 ; 4(1) : 46-56.   DOI
32 Cooper C, Campion G, Melton LJ 3rd. Hip fractures in the elderly: a world-wide projection. Osteoporos Int. 1992 ; 2 : 285-9.   DOI
33 Kennel KA and Drake MT: Adverse effects of bisphosphonates: implications for osteoporosis management. Mayo Clin Proc. 2009 ; 84(7) : 632-637.   DOI
34 J W Hong, W Nam, I-H Cha, S-W Chung, H S Choi, K M Kim, K J Kim, Y Rhee, S-K Lim. Oral bisphosphonate-related osteonecrosis of the jaw: the first report in Asia. Osteoporos Int. 2010 ; 21(5) : 847-53.   DOI
35 金達鎬, 李鍾馨. 黃帝內經 素問. 서울:醫聖堂. 2001 ; 552-553, 908-909.
36 Rauner M, Sipos W, Thiele S, Pietschmann P. Advances in osteoimmunology: pathophysiologic concepts and treatment opportunities. Int Arch Allergy Immunol. 2013 ; 160 : 114-125.   DOI
37 Charles JF, Nakamura MC. Bone and the innate immune system. Curr Osteoporos Rep. 2014 ; 12:1-8.   DOI