• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.4
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• pp.337-347
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• 2017

In this study, free radical scavenging activity and enzyme-linked immunosorbent assay (ELISA) experiments were carried out using Dioscorea japonica (D. japonica) and Chenopodium album (C. album) extracts to evaluate their anti-oxidative and anti-inflammatory effects. In the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity, $EC_{50}$ of D. japonica and C. album were measured as 2.386 and 0.524 mg/mL, respectively. The free radical scavenging activity of the mixed sample of D. japonica and C. album was the highest when the D. japonica and C. album ratio was 2 : 1. The IL-6 and $TNF-{\alpha}$ ELISA assay showed that IL-6 in mouse spleen cells treated 1 mg/mL of samples, D. japonica and C. album decreased the production of IL-6 concentration by 27.17%, 72.30%. In the case of $TNF-{\alpha}$, D. japonica and C. album decreased 61.97% and 77.85% of $TNF-{\alpha}$ production, respectively. Through these results, we confirmed that D. japonica and C. album have antioxidant and anti - inflammatory effects and could be applied to natural medicine cosmetic having anti - inflammatory effects.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.31-38
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• 2014

The fish community and upstream of glass eels (Anguilla japonica) in the Imjin River estuary Gyeonggi-do, Korea was investigated from 2008 to 2012. During the surveyed period 43 species belonging to 18 families were collected. Korean endemic species was Microphysogobio jeoni which showed a ration of 0.04% in collected species. Exotic fishes were Carassius cuvieri and Ctenophayngodon idellus, and comprised 0.06% of the total individual number. Among the fish species observed, 17 species of the freshwater fish (40.5%), 6 species of migration fish (14.2%), 8 species of the brackish water fish (19.1%) and 11 species of sea water fish (26.1%). In terms of composition ration, Anguilla japonica (glass eels, 49.8%), Coilia ectens (23.3%), Saurogobio dabryi (8.1%), Repomucenus olidus (3.3%), Lateolabrax maculata (2.9%) and Chelon haematocheilus (2.8%) were found to display high individual number. On the other hand, C. ectens (33.6%), C. haematocheilus (14.2%), Cyprinus carpio (10.5%), Mugil cephalus (9.1%) and S. dabryi (7.6%) were dominated of biomass. The dominant species of individual was A. japonica (glass eels), and subdomint species included C. ectens. The fish biomass catched of fisherman in the Imjin River (Paju-si) were 83.6~240.3 t by each year. The year 2011 and 2012 were decreased rapidly. Among these, M. cephalus (48.0~80.0%), C. carpio (6.7%), Carassius auratus (4.9%), Silurus asotus (3.9%), Takifugu obscurus (2.5%), Anguilla japonica (adult, 2.2%) and Pseudobagrus fulvidraco (2.1%) dominated of biomass. Body weight of glass eels at each year were 0.03~ 1.13 t, average 212.0 kg and 1,325,000 individual by 0.16 g each one individual.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1209-1218
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• 2021

The aim of this study was to assure the possiblility of Achyranthes japonica Nakai for a cosmetic material. For this we examined the Anti-oxidation, Anti-inflammation, Anti-wrinkle effects of Achyranthes japonica Nakai extract. Each material is extracted from Achyranthes japonica Nakai roots(AJNR) and Achyranthes japonica Nakai stalks(AJNS) using 70% ethanol. We carried out Nitric oxide assay of the extract with cultured RAW 264.7 macrophage cells, And we conducted MMP-1 assay, Type I procollagen synthesis assay of the extract with cultured CCD-986sk fibroblast cells. After we measured ABTS+ assay and SOD assay of AJNR and AJNS, we knew that effect of anti-oxidant increased as their concentration get higher. The result of this study was that the antioxidant activity of AJNR and AJNS was similarly excellent and the anti-inflammatory activity of AJNR was significantly higher than AJNS. The MMP-1 inhibitory activity and Type I procollagen synthesis of AJNR and AJNS was generally excellent, AJNS had slightly better than AJNR. Therefore I thought it has the chance to play an imfortant role as a functional cosmetic material for anti-oxidant, anti-inflammation and wrinkle reduction effects.

• Korean journal of applied entomology
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• v.58 no.1
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• pp.77-83
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• 2019

This study was conducted to investigate the developmental characteristics of Propylea japonica Thunberg (Coleoptera: Coccinellidae) and its consumption of Myzus persicae nymphs at 3 constant temperatures (20.0, 25.0 and $30.0^{\circ}C$; $60{\pm}5%$ relative humidity; 14 h light : 10 h dark). The longevity of adult female P. japonica under 20, 25, and $30^{\circ}C$ was 134.0, 101.0 and 55.2 days, respectively. The total fecundity was 508.6, 875.6, and 383.4 eggs during its life span, respectively. The longevity of adult male P. japonica under 20, 25, and $30^{\circ}C$ was 128.8, 97.8, and 46.5 days, respectively. Average daily consumption of adult M. persicae by 1st, 2nd, 3rd, and 4th instar P. japonica at $25^{\circ}C$ was 2.2, 7.3, 14.5, and 29.1, respectively. The average daily number of M. persicae consumed by male and female P. japonica over their lifetimes was 35.0 and 42.9, respectively. Average daily consumption of adult Aphis gossypii by 1st-4th instar P. japonica at $25^{\circ}C$ was 2.2, 7.5, 13.9, and 29.5, respectively. The average daily number of A. gossypii consumed by male and female P. japonica over their lifetimes was 37.0 and 40.8, respectively.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.34-39
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• 2014

In this study, the community structures of arbuscular mycorrhizal fungi (AMF) in rhizospheres of Camellia japonica and neighboring woody plants in Wando, Korea were investigated. Rhizospheres of C. japonica and other woody plants were dominated by the same species, Acaulospora mellea, but Shannon's index, species richness and total spore numbers of the AMF communities were higher in non-C. japonica than in neighboring plants. Regardless of host plant species, the frequency of A. mellea was significantly high comparing with other AMF species. The community similarity of AMF within C. japonica was significantly higher than between C. japonica and neighboring plants or neighboring plants (p<0.005). Results showed that AM fungal communities in rhizospheres of C. japonica have unique community structure and are different from that of neighboring host plants, suggesting that community structure of AMF could be influenced by host plant species.

• The Journal of the Korea Contents Association
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• v.20 no.6
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• pp.124-130
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• 2020

The present study investigated the anti-proliferate and anti-invasive of Phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase (MMP-2) and MMP-9 activities of combined treatment with cisplatin and ethyl acetate fractions of Paeonia japonica. Cell Proliferation was detected by the MTS assay and the activity and mRNA expression of MMP-2/-9 were examined by zymography and RT-PCR. As results, cisplatin or p. japonica treatment of YD-10B cells resulted in a dose-dependent inhibition of cell growth. Also, the viability of YD-10B cells treated with combination of 200 μM cisplatin and 50 ㎍/ml p. japonica was inhibited to 50% in compared with the cisplatin alone. In PMA-treated YD-10B cells, co-treatment of 200 μM cisplatin with 50 ㎍/ml p. japonica significantly inhibited mRNA expression and protein activation of MMP-2/-9. Therefore, This study suggest that the combination treatment of cisplatin and p. japonica potentiates a promising anti-invasive agent and has more potential anti-cancer drug for oral cancer therapy than cisplatin alone.

• The Korean Journal of Food And Nutrition
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• v.28 no.3
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• pp.343-350
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• 2015

The aim of this study was to evaluate the potential anti-inflammorty properties of herbs via MAPKs such as ERK, p38, JNK. Fifty-one kinds of each herbal medicine, that were extracted with ethanol, were used in the inhibitory assay of cytokine (TNF-${\alpha}$, IL-$1{\beta}$ and IL-6) and NO. of these, 10 species of herbal medicines, Angelica dahurica, Atractylodes lancea, Cnidium officinale, Duchesnea chrysantha, Oldenlandia diffusa, Lonicera japonica, Paeonia lactiflora, Pinus thunbergii, Rehmannia glutinosa and Rubus coreanus, were screened as potential inhibitors (< $300{\mu}g/mL$) of NO, TNF-${\alpha}$, IL-$1{\beta}$ and IL-6. Among the 10 species, Lonicera japonica showed potential anti-inflammatory effects Lonicera japonica extract of $200{\mu}g/mL$ inhibited the phosphorylation of ERK, p38 and JNK. In addition, Lonicera japonica extract at 20 mg/kg increased survival rate from LPS-induced endotoxin shock by 3 fold.

• The Korea Journal of Herbology
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• v.26 no.2
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• pp.1-10
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• 2011

Objective ： Lonicera japonica contains anti complementary polysaccharides and polyphenolic compound. Among these polyphenolic substances, chlorogenic acid is the major active component of this plant. However, the immunological mechanisms for these activities, have not been elucidated, nor the active components. To clarify immunomodulatory effects of those we examined the relationship between the activity of CD8+ T cell-mediated lysis and the frequency of cytokine profiles in spleen, thymus (especially IFN-${\gamma}$, IL-4, GM-CSF etc.) expressing CD8+ T cells activated by IL-2. Methods : To study immunomodulatory effects ethyl acetate fraction from Lonicera japonica, chlorogenic acid on cytokine gene expression from spleen, thymus cells, RT-PCR was performed after quantitative normalization for each gene by a densitometry using ${\beta}$-actin gene expression. A modified standard $^{51}Cr$-release assay was used to measure cytotoxic activities of cytotoxic T cells. Spleen, thymus cells from NOD mice were stained with CD3, CD4, CD44, CD69 in staining buffer and analyzed by two color flow cytometry. Results : We showed that ethyl acetate fraction from Lonicera japonica in combination with IL-2 resulted in a significant enhancement of PCR products for IFN-${\gamma}$, IL-4, IL-10, GM-CSF, IL-6 and cytotoxtic CD8+ T cell proportion in spleen and thymus T cells in NOD mice. This suggests that IFN-${\gamma}$, IL-6 like IL-4 may be acting as a regulatory rather than proinflammatory cytokine. Conclusions : In conclusion, based on the results of the present study which showed that ethyl acetate fraction from Lonicera japonica and chlorogenic acid upregulating cytokine gene expression in spleen and thymus, we are tempted to speculate that some of the therapeutic efficacies such as anti-diabetic activity of Lonicera japonica are due to the immunomodulatory its ethylacetate fraction and chlorogenic acid.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.316-321
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• 2003

Analysis of breeding gains in grain yield has been intensively conducted in wheat, barley, oat, maize, and soybean. Such information is limited in rice. The objective of this study was to compare the breeding gains and cultural gains contributed to yield gains of Korean rice varieties since early 1900s. Two sets of yield data were used for analysis; the historical yield data of 1908 for old japonica cultivars, and present yield data in the years from 1996 to 1998 for the six cultivars, consisting of previous two old cultivars and four contemporary cultivars. The old cultivars were two native cultivars, Jodongi and Damageum, while contemporary cultivars were two premium quality japonica cultivars, Hwaseongbyeo and Dongjinbyeo, and two Tongil-type cultivars, high yielding cultivars developed from indica/japonica hybridization, Milyang23 and Dasanbyeo. The yield differences of old cultivars between the experiments in 1908 and the experiments from 1996 to 1998 were estimated as cultural gains (1.84 tons $\textrm{ha}^{-1}$) due to the improvement of cultivation technology. Yield differences between the old cultivars and contemporary cultivars were considered total yield gains during the periods. These were 2.51 tons $\textrm{ha}^{-1}$ for japonica cultivars and 3.81 tons $\textrm{ha}^{-1}$ for Tongil-type cultivars. From these data, the genetic gain of 0.67 tons $\textrm{ha}^{-1}$ and 1.97 tons $\textrm{ha}^{-1}$ were estimated for japonica cultivars and Tongil-type cultivars respectively. The ratio between cultural gain and genetic gain appeared to be 2.7:1 for japonica cultivars and 1:1 for Tongil-type cultivars. This analysis clearly showed the higher genetic contribution in Tongil-type cultivars than in japonica cultivars, suggesting a guideline to be used when planning new yield improvement programs. Additional implication has emerged when a better yield response to modem cultivation technology was found in one of the old cultivars, suggesting the combined improvement between breeding and cultural improvement is necessary for attaining the maximum yield capacity of a crop.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.147-153
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• 1991

In order to clarify the varietal differences of grain shedding in 86 rice varieties with the diverse origin, a bundle of 10 panicles was fallen down on the bottom above 2m height, and several characters related to shattering were investigated. The ratio of grain shedding in the varieties from the Tongil types was the greatest (11. 29%), followed by the native and J aponica types (2.56, 0.17%). The breaking tensile and breaking bending strengths of the primary rachis-branch in the Tongil types were weaker than in the native and Japonica types with mean values of 217g and 20.5g, respectively. The fiber cells length of the primary rachis- branch in the Tongil types were shorter in the native and Japonica types. The ratio of grain shedding was significantly correlated with the breaking tensile and bending strengths of the primary rachis- branch, and fiber cell length.