• The Korean Journal of Pesticide Science
• /
• v.17 no.4
• /
• pp.411-418
• /
• 2013

The black pine bast scale, Matsucoccus thunbergianae is one of the most serious insect pest in Japanese black pine, Pinus thunbergii forest in Korea. Insecticidal activity of 10 folds hot water extracts from 7 herbal plants (Atractylodes lancea, Eugenia caryophyllata, Lonicera japonica, Melia azedarach, Quisqualis indica, Sophora flavescens and Taraxacum mongolicum) were tested against different stage of M. thunbergianae using spray method both in laboratory and field. Efficacies of herbal extracts were different depending on stage of M. thunbergianae. Q. indica sprayed with hand sprayer produced the highest corrected mortality of 95.7% on intermidiated nymph stage, however, A. lancea produced the highest corrected mortality (51.3%) on pupae of M. thunbergianae in laboratory. Q. indica and A. lancea were highly effective against female M. thunbergianae in laboratory, however, corrected mortality was lower than other stage (40.0%). Efficacy of Q. indica was similar to effective insecticide, fenitrothion 50% EC against M. thunbergianae in field trials. These results indicated that Q. indica could be an environmental friendly control agent of M. thunbergianae.

• Journal of Korean Society of Forest Science
• /
• v.96 no.5
• /
• pp.585-590
• /
• 2007

Rhizina undulata is the fungus, which causes Rhizina root rot on coniferous trees. Nested-PCR using ITS-specific primer was applied to detect R. undulata from the soils of Japanese black pine (Pinus thunbergil) forests infested with the disease in Seocheon, Chungnam Province, South Korea. Soil samples were collected from four different sites, both dead trees and fruit bodies of R. undulata were present, dead trees only present, fruit bodies only present, and both were absent. Nested-PCR products specific to R. undulata ITS-region were amplified. Positive reactions were found in some samples from the sites, where dead trees and fruit bodies of R. undulata were absent as well as where both of those were present. R. undulata was mainly detected in the soil samples from the depth of 5~20 cm under the soil surface. These results show that the nested-PCR could be used to diagnose the presence or potential infestation of R. undulata in the soils of pine forests.

• Weed & Turfgrass Science
• /
• v.2 no.1
• /
• pp.30-37
• /
• 2013

The objective of this research was to understand herbicidal activity of essential oils isolated from leaves of pine (Pinus densiflora), nut pine (Pinus koraiensis), larch (Larix kaempferi) and khingan fir (Abies nephrolepsis) in Korea. In a seed bioassay, essential oils of nut pine, larch and khingan fir inhibited the growth of rapeseed (Brassica napus) seedlings by 50% at 4,766, 1,865, $5,934{\mu}g\;ml^{-1}$, respectively, however, that of pine did not show any herbicidal effect. In a green house experiment, fall panicum, Southern crabgrass, sorghum, barnyardgrass, quackgrass, black nightshade, Indian jointvetch, velvet leaf, and Japanese morningglory were controlled in 24 hours by the foliar application of 10% essential oils from pine, nut pine, larch and khingan fir. The treated plant parts showed burndown effect, however, new shoots appeared 3 days after treatment. Results of GC-MS analysis showed that essential oils from pine, nut pine, larch and khingan fir contained 16, 25, 25, and 16 compounds, respectively, with hydrocarbons, alcohols, ketones, and esters. The major compounds of the essential oils were 3-carene, bornyl acetate, camphene, limonene, ${\alpha}$-pinene, ${\beta}$-pinene and ${\beta}$-phellandrene.

• Korean Journal of Organic Agriculture
• /
• v.21 no.4
• /
• pp.629-646
• /
• 2013

This study was conducted to investigate effects of terpenoids-rich plant extracts (TRPE) on the in vitro ruminal fermentation characteristics and methane production. The ruminal fluid was collected from a cannulated Hanwoo cow fed concentrate and timothy in the ratio of 6 to 4. The TRPE as Mint (Mentha arvensis var. piperascens), Pine (Pinus densiflora), Japan cedar (Cryptomeria japonica), Sichuan pepper (Zanthoxylum piperitum), Hinoki cypress (Chamaecyparis obtuse) and Japanese black pine (Pinus thunbergii) were used in this study. The 15 mL of mixture, contains McDougall buffer and rumen fluid in the ratio of 2 to 1. The mixture was dispensed anaerobically 50 mL serum bottles and it is contained 0.3 g timothy substrate and 5% TRPE. The bottles were incubated at $39^{\circ}C$ for 3, 6, 9, 12, 24, 48 and 72 hours. The pH value decrease by increased incubation times and the pH values at all times were significantly (p<0.05) higher in treatments than in control. The digestibility of dry matter at 3 hours was significantly (p<0.05) higher in mint treatment than in control. Productions of total gas and carbon dioxide at before 12 hours was significantly lower (p<0.05) in treatments than in control. The methane production at 24 hours was significantly (p<0.05) lower in treatments than in control. The concentrations of acetic acid and propionic acid at 24 hours were significantly higher (p<0.05) in mint and pine treatments than in control. In conclusion, the terpenoid-rich plant extracts were shown to decreased methane emission and without adversely affected ruminal fermentation. Therefore, the terpenoid-rich plant extracts as mint and pine were shown to decreased methane production and it has potential possibility for ruminal fermentations.

• The Korean Journal of Pesticide Science
• /
• v.13 no.4
• /
• pp.267-274
• /
• 2009

This study was carried out to select effective preventive pesticides against pine wilt disease caused by pinewood nematode (PWN), Bursaphelenchus xylophilus on trunk injection. 1,000 fold aquatic solution of abamectin 1.8% EC and emamectin benzoate 2.15% EC were lower mortality (7.3% and 8.3% respectively) against PWN on 1 day after treatment. However effects of abamectin 1.8% EC, emamectin benzoate 2.15% EC, fosthiazate 30% SL and fenitrothion 30% SL were inhibited the reproduction of PWN over 99.6% in Botrytis cineria media. Effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in diameter of breast height (DBH) on mortality of Japanese black pine, Pinus thungergii by inoculated PWN was 0% and 3.3%, respectively at the applied year however when injection of fosthiazate 30% SL were treated with the rate of $5\;m{\ell}$ per 10 cm tree DBH, mortality of tree was 63.3%. Abamectin 1.8% EC and emamectin benzoate 2.15% EC was showed high preventive efficacy representing >90% against PWN at the following year. PWN preventing efficacy of trunk injection was lower in naturally occurred area (mortality of pine tree in control was 11.7% at the first year) of PWN than artificially infected site (mortality of pine tree in control was >76.7% at the first year), PWN preventing efficacy of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in DBH was 91.5% and 82.9%, respectively, at the applied year and 89.5% and 82.6% respectively at the following year in PWN naturally occurred site. Control efficacy by trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC was more higher in 10 fold dilution with 10 fold high amount of aquatic solution than no dilution with 10 fold less amount of aquatic solution. The preventive effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $5\;m{\ell}$ per 10 cm in DBH was showed 100% at the applied year in PWN inoculated tree.

• The Korean Journal of Pesticide Science
• /
• v.20 no.1
• /
• pp.56-65
• /
• 2016

Pine wood nematode, Bursaphlenchus xylophilus is a fatal damaging pest, infecting pinewood trees and is one of the serious forest pest in Korea. Trunk injection is one of the most effective method to protect the live pine trees from nematode infection. The aim of this research was to reveal the efficacy of the nematicidal materials from 46 Kyrgyzstan, 88 Vietnam plant materials and 21 active materials (${\alpha}$-terpinene, ${\alpha}$-pinene, ${\beta}$-thujaplicin, cinnamaldehyde, eugenol, emodin, geraniol, limonone, methyl palmitate, matrine, myrcene, methyl gallate, nicotine, quassin, resveratrol, rotenone, thymol, thujaplicin, terthiophene, tuberstemonine, ${\gamma}$-terpinene) isolated from plants for using as trunk injection agents. Eclipta prostrata extract was highly effective against pine wood nematode in plant extracts and terthiophene has the best nematicidal activity from active materials isolated from plants. Foliar spray of terthiophene on Japanese black pine (Pinus thunbergii) tree reduced proliferation of inoculated pine wood nematode at 22 days after treatment, however no efficacy was found over 22 days. Eclipta prostrata extract and terthiophene can be used as nematicide for further nematicidal efficacy test against any other plant parasitic nematodes and trunk injection efficacy against pine wood nematode.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
• /
• v.2 no.2
• /
• pp.135-138
• /
• 1998

This area is plant-geographically located at the Temperate Subtropical Zone and Evergreen Broad Leaf Zone which is included in the sea of southern area of the Korean Peninsula. The Japanese black pine is the most common tree in this area. The sorts of trees that are living in this area Camellia, Alder, Oak, Acorn. It is reported that this area is located at the Evergreen Broad Leaf Zone of Korean Peninsula that is living in the southern sea. The construction place was originally a grassy place. About 40 familly 100 species different plants were living at this area. The most common animals were the Rodents and the most common birds were the Colombiformes.

• The Korean Journal of Pesticide Science
• /
• v.19 no.4
• /
• pp.440-449
• /
• 2015

Pine wood nematode (PWN), Bursaphelenchus xylophilus is one of the most serious pests of pine tree. Trunk injection of some nematicides in tree is well known as an effective control method. However there are some limitating factors which hindering the efficacy of trunk injection in field or potted tree. In this study we suggested easy and useful alternative screening methods of nematicides against PWN. Reproduction of PWN was influenced by tree twig moisture (high reproduction in high moisture trig) and paraffin coating was 78.6% reduced moisture loss in tested twig. There were no reproduction different in up and down site from infection site of twig at 1 month after inoculation of PWN and also distance (5 and 10 cm) from inoculation site of PWN did not influence the reproduction of PWN. Numbers of reproduced PWN were higher with decreasing diameter of twig. Numbers of reproduced PWN were similar to P. thunbergii and P. densiflora. However reproduction was increased depending on high inoculation density and longer propagation period. When inoculation of PWN on cut twig injected with emamectin benzoate 2.15% EC and morantel tartrate 8% SL in trunk of Pinus thunbergii in the field, PWN number were significantly reduced than untreated control. We suggest this screening method for PWN control agents.

• Korean Journal of Environment and Ecology
• /
• v.29 no.4
• /
• pp.599-604
• /
• 2015

This study was conducted the vegetation clustering analysis for the 3 mountains of Mt. Bulam, Mt. Daemo, Mt. Bonghwa which were the urban forests of Seoul. Based on the results of the analysis related to the vegetation clustering, it was found that the clustering of Mt. Bulam consisted of pine trees(Pinus densiflora)-Mongolian oak(Quercus mongolica), Hornb eam(Carpinus laxiflora)-Pitch pine(P. rigida), oriental oak(Q. variabilis) - a wild pear tree(Sorbus alnifolia) while the clustering of Mt. Daemo consisted of Pitch pine-Japanese larch(Larix leptolepis), Poplar(Populus tomentig landulosa)- black birch(Betula davurica pall). Meanwhile, the clustering of Mt. Bonghwa consisted of pine trees-a wild pear tree Community and Sawtooth oak(Q. acutissima)-Cherry Blossoms(Prunus serrulata). In relation to the similarity index by region in Mt. Bulam, Mt. Daemo, and Mt. Bonghwa, the similarity index of Mt. Bulam and Mt. Daemo stood at as high as 0.634, suggesting the distribution of similar vegetation, and the dominance index of the Mt. Daemo region was found to be 0.166 which suggests the dominance of many species compared to other regions. In addition, the results of species diversity showed that Mt. Daemo had the highest stability, and the species diversity, maximum species diversity, evenness indices were highest in Mt. Bulam, followed by Mt. Bonghwa and Mt. Daemo. The dominance index was the lowest in Mt. Bulam, followed by Mt. Bonghwa and Mt. Daemo.

• 보존과학연구
• /
• s.32
• /
• pp.53-60
• /
• 2011

The aim of this study is to investigate the traditional manufacturing technique of lacquering by comparing and analyzing materials used for wooden lacquerware artifacts with focus on the Joseon dynasty Eugye, and to apply the manufacturing technique to the conservation treatment of the lacquerware. artifacts. This study conducted the documentary survey focusing on "Yongjo Jeongsun Queen Consort Garye Dogam Eugye",and "Jeongjo Gukjang Dogam Eugye." The royal lacquering is roughly classified into three categories-scarlet lacquering, quality black lacquering, and terra rosa lacquering- according to materials used for lacquerware. The bean flour was mostly used for under lacquering of the articles which was offered to the king and in case of other articles, bean flour was used mixed with bone ashes. In some cases, burned pine soot was added to under lacquering. Japanese scarlet lacquering was applied by mixing maechil-main coat, in inverted commas- with Japenese scarlet after lacquering jeonchil-base coat, in inverted commas-. In case of Chinese scarlet lacquering, part of terra rosa was used mixed with red clay. For quality black lacquering, basically jeonchil and maechil were used, but sometimes part of burned pine soot was added. In addition, terra rosa lacquering was applied by basically using terra rosa mixed with red clay. Lastly, gloss was given by using perilla oil. It is expected that the traditional materials and technique examined through both documentary and relic research will contribute to advanced result of the conservation treatment of wooden lacqueringware artifacts.