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Detection of Rhizina undulata in Soil by Nested-PCR Using rDNA ITS-specific Primer  

Lee, Sun Keun (Department of Forest Resources Protection, College of Forest and Environmental Sciences, Kangwon National University)
Lee, Jong Kyu (Department of Forest Resources Protection, College of Forest and Environmental Sciences, Kangwon National University)
Lee, Seung Kyu (Division of Forest Insect Pests and Disease, Korea Forest Research Institute)
Kim, Kyung Hee (Division of Forest Insect Pests and Disease, Korea Forest Research Institute)
Lee, Sang Yong (Department of Forest Resources Protection, College of Forest and Environmental Sciences, Kangwon National University)
Publication Information
Journal of Korean Society of Forest Science / v.96, no.5, 2007 , pp. 585-590 More about this Journal
Abstract
Rhizina undulata is the fungus, which causes Rhizina root rot on coniferous trees. Nested-PCR using ITS-specific primer was applied to detect R. undulata from the soils of Japanese black pine (Pinus thunbergil) forests infested with the disease in Seocheon, Chungnam Province, South Korea. Soil samples were collected from four different sites, both dead trees and fruit bodies of R. undulata were present, dead trees only present, fruit bodies only present, and both were absent. Nested-PCR products specific to R. undulata ITS-region were amplified. Positive reactions were found in some samples from the sites, where dead trees and fruit bodies of R. undulata were absent as well as where both of those were present. R. undulata was mainly detected in the soil samples from the depth of 5~20 cm under the soil surface. These results show that the nested-PCR could be used to diagnose the presence or potential infestation of R. undulata in the soils of pine forests.
Keywords
Rhizina undulata; ITS-specific primer; nested-PCR;
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