• Parasites, Hosts and Diseases
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• 제29권1호
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• pp.67-86
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• 1991

경북지역에서의 뇌염매개모기, 작은빨간집모기 (Culex tritaeniorhynchus)의 계절적 출현 소장과 군집변동이 일본뇌염 발생과 유행에 미치는 영향을 규명하기 위해서 1984년부터 1990년까지 경산군 1 개소에 유문등으로 1주일에 한번씩 성충을, 1990년 4월부터 9월까지 경북농촌진흥원 부속농장에서 모기유충을 채집하였다. 1984년부터 1990연까지 유문등에 처음으로 작은빨간집모기가 채집되는 날짜는 연도별로 큰 차이를 나타내었으며, 6월 4일부터 28일 사이에 채집되었고, 가장 높은 군집밀도를 보인 것은 8월 중순에서 9월초였고, 그 후 점점 감소하여 10월 말에는 채집되지 않았다. 작은빨간집모기의 채집되는 수는 1985년 이후 급격히 감소하여, 1989년에는 가장 적은 수가 채집되었다. 첫 3년 동안은 최고 군집밀도가 8월에 관찰되었고, 그 이후해에서 모기수의 감소와 함께 늦어지는 것을 알았다. 야간활동성은 저녁 8∼9시 사이에 가장 왕성하며, 그 후 점차 감소하다가 새벽 4∼5시 다시 약간 증가하였다. 작은빨간집모기 유충은 7월 중순에 논에서 처음으로 발견되었고, 그 밀도는 8월 중순에 가장 높았으며, 최고 군집밀도는 1m2당 평균수는 14,900마리였다. 9월 하구 이후부터는 그 밀도가 현저히 감소하였다. 작은빨간집모기 유충은 5종의 유기린제에 대하여 높은 저항성을 나타내었다. 곤충 사육실내에서 작은빨간집모기, 경산 종의 생명표 특성에서 평균수명은 암컷은 59.8일이었고, 수컷은 28.3일이 었으며, Net reproductive rate는 7.8, Generation time은 15.5일였다.

• Pediatric Infection and Vaccine
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• 제25권3호
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• pp.132-140
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• 2018

목적: 뎅기바이러스 감염 환자 발생은 해외 여행이 증가함에 따라 증가하고 있다. 최근 감염 경험이 없는 사람에게 백신을 접종하면 이후 야생 뎅기바이러스 감염 시 중증 뎅기열 증상을 보일 수 있다고 연구된 바 있다. 따라서, 본 연구는 한국의 다양한 연령군에서 뎅기바이러스에 대한 혈청역학을 연구하였다. 방법: 건강한 영아 98명(2개월-1세), 청소년 152명(13-19세), 성인 90명(20-50세) 및 노인 106명(65세 이상)에서 수집한 혈청 총 446명을 대상으로 하였다. 각 연령군의 뎅기바이러스 immunoglobulin G (IgG) 항체 검사를 ELISA을 통해 측정하였다. 또한 뎅기바이러스 IgG 항체 검사에서 양성 또는 equivocal을 보이는 혈청에 한하여 일본뇌염 바이러스의 IgG 항체를 검사하였다. 결과: 총 446명 검체 중, 청소년군에서 1명(0.2%)만 뎅기바이러스 항체 검사에서 양성으로 나왔다. Equivocal은 14명(3.1%)으로, 청소년군 10명과 노인군 4명이 해당하였다. 뎅기바이러스 IgG 양성이 나온 1명에서 일본뇌염 바이러스 또한 IgG 양성으로 나왔다. 뎅기바이러스 IgG equivocal이 나온 14명에서는 일본뇌염 바이러스 IgG 양성이 6명, equivocal이 3명이었고 음성은 5명이었다. 결론: 한국인에서 뎅기바이러스에 대한 항체 보유율은 매우 낮았다. 본 연구는 향후 뎅기열 예방을 위한 보건 정책 수립에 중요한 자료가 될 수 있을 것이며 향후 지속적인 혈청면역 평가도 필요할 것이다.

• 대한수의학회지
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• 제10권2호
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• pp.59-66
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• 1970

Studies were conducted on the survey of HI antibody abortion and stillbirth from J.E. virus in swine for 1968-1969 in Korea HI antibody against J.E. virus showed postive in June or July and reached 80~100 percent of postive in August or September and J.E. virus strains were isolated from the brain material of the aborted piglets.

• 한국동물위생학회지
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• 제27권3호
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• pp.281-288
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• 2004

As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.83-86
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• 2003

Major advances in positive-sense RNA virus research have been facilitated by the development of reverse genetics systems. These systems consist of an infectious cDNA clone that encompasses the genome of the virus in question. This clone is then used as a template for the subsequent synthesis of infectious RNA for the generation of synthetic viruses. However, the construction of infectious cDNA for the Japanese encephalitis virus (JEV) has been repeatedly thwarted by the instability of its cDNA. As JEV is an important human pathogen that causes permanent neuropsychiatric sequelae and even fatal disease, a reliable reverse genetics system for this virus is highly desirable. The availability of this tool would greatly and the development of effective vaccines as well as facilitate studies into the basic biology of the virus, including the molecular mechanisms of viral replication, neurovirulence, and pathogenesis. We have successfully constructed a genetically stable infectious JEV cDNA containing full-length viral RNA genome. Synthetic RNA transcripts generated in vitro from the cDNA were highly infectious upon transfection into susceptible cells, and the cDNA remained stable after it had been propagated in E. coli for 180 generations. Using this infectious JEV cDNA, we have successfully expressed a variety of reporter genes from the full-length genomic and various subgenomic RNAs in vitro transcribed from functional JEV cDNAS. In summary, we have developed a reverse genetics system for JEV that will greatly facilitate the research on this virus in a variety of different fields. It will also be useful as a heterologous gene expression vector and aid the development of a vaccine against JEV.

• 대한바이러스학회지
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• 제27권2호
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• pp.197-207
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• 1997

To investigate the NS4 region of JEV, NS4 cDNA of K94P05 (JEV strain isolated from Korea in 1994) was amplified by RT-PCR and analyzed by sequencing PCR product. Genomic size of NS4 was 1212bp and nucleotide sequence was compared with that of other JEV strains. Nucleotide homology between JaOAr582 and K94P05 was 91.1% and that between Beijing and K94P05 was 89.8%, respectively. But the nucleotide sequence of E region of JaOAr582 and K94P05 showed 97.0% homology and that of Beijing and K94P05 did 95.8% homology. NS4 protein was expressed as a form of fusion protein by a prokaryotic expression system. The induced fusion product showed a lower molecular weight than predicted size and remained insoluble. The NS4 protein might be cleavaged by E. coli protease. Concluding above results, high hydrophobicity of the NS4 protein supported the fact that this protein played a role as a membrane component and the poor nucleotide sequence conservativity among JEV strains suggested that this region might be important to adapt each viral growth environment.

• 한국동물위생학회지
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• 제46권2호
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• pp.115-122
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• 2023

This study was performed to investigate the distribution of mosquito vectors related to the zoonotic disease in Daejeon. Samples were taken using a blacklight trap once a month from March to November 2021 at the slaughterhouse in Daejeon. A total of 820 mosquitoes were captured and classified into 5 genera and 8 species. Among the collected mosquitoes, 319 (38.9%) and 295 (35.93%) were Aedes vexans nipponii and Culex pipiens pallens, respectively, making them the dominant species. The overall number of mosquitoes collected started to increase from May and reached the largest value of 329 (40.12%) in June. Trapped mosquitoes are created 72 pools by environmental condition and by species. The pools were tested by PCR methods for 7 zoonotic pathogens. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in 3 pools collected from cow lairage (Culex pipiens pallens) in May, cow by-product processing room (Aedes vexans nipponii) in June and cow lairage (Mansonia uniformis) in June. Culex flavivirus were detected in 4 pools. Based on the results of this study, it is considered that continous surveillence of mosquitoes in livestock assembly facilities (slaughterhouse) should be performed for controlling mosquito populations and mediating disease spread by mosquitoes.

• Pediatric Infection and Vaccine
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• 제23권1호
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• pp.1-9
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• 2016

The Zika virus, a flavivirus related to dengue and Japanese encephalitis was discovered in the Zika forest in Uganda, 1947. Since Zika virus was first reported in Brazil in May 2015, infections have occurred in at least 40 countries, especially in the Americas. Zika virus infection usually is asymptomatic or causes mild illness, but may be related to severe clinical manifestations, particularly microcephaly and Guillain-$Barr{\acute{e}}$ syndrome. Although the possibility of autochthonous Zika virus transmission in South Korea is low, the imported cases and Zika virus-transmitting mosquito should be adequately monitored and promptly managed. In addition, enhancing preparedness for Zika virus infection are needed.

• 대한수의학회지
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• 제32권3호
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• pp.365-368
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• 1992

1988년부터 1990년 6월까지 전국의 양돈장에서 수집된 돼지 유사산 태아 74복에서 바이러스성 원인체 분리 및 혈청학적 진단을 수행하였던 바 다음과 같은 결과를 얻었다. 공시한 74복의 유사산 태아중 44복의 태아 흉강액에서 면역 globulin이 검출되어 전염성 질병감염에 의한 유사산으로 추정되었다. 이중 37%가 바이러스성 유사산으로 나타났으며 유사산의 원인체별 분포를 살펴보면 돼지 파보바이러스가 21%로 가장 높았으며, 뇌심근염 바이러스가 11%, 일본뇌염 바이러스가 9% 등의 순으로 나타났다. 한편 돼지 콜레라바이러스 및 오제스키병 바이러스에 의한 유사산이 각각 1건씩 검출되었으며 동일 유사산 태아에서 2가지 병원체가 중복감염된 예도 관찰되었다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.230.4-230
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• 2005