Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and identification of mosquito-borne zoonotic diseases in slaughterhouse in Daejeon

  • Youngju Kim (Daejeon Institute of Health and Environment Division of Animal Health) ;
  • Gyurae Kim (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Sunkyong Song (Daejeon Institute of Health and Environment Division of Animal Health) ;
  • Youngshik Jung (Daejeon Institute of Health and Environment Division of Animal Health) ;
  • Seojin Park (Daejeon Institute of Health and Environment Division of Animal Health) ;
  • Sang-Joon Lee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Ho-Seong Cho (College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University) ;
  • Yeonsu Oh (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • Received : 2023.05.28
  • Accepted : 2023.06.09
  • Published : 2023.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the distribution of mosquito vectors related to the zoonotic disease in Daejeon. Samples were taken using a blacklight trap once a month from March to November 2021 at the slaughterhouse in Daejeon. A total of 820 mosquitoes were captured and classified into 5 genera and 8 species. Among the collected mosquitoes, 319 (38.9%) and 295 (35.93%) were Aedes vexans nipponii and Culex pipiens pallens, respectively, making them the dominant species. The overall number of mosquitoes collected started to increase from May and reached the largest value of 329 (40.12%) in June. Trapped mosquitoes are created 72 pools by environmental condition and by species. The pools were tested by PCR methods for 7 zoonotic pathogens. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in 3 pools collected from cow lairage (Culex pipiens pallens) in May, cow by-product processing room (Aedes vexans nipponii) in June and cow lairage (Mansonia uniformis) in June. Culex flavivirus were detected in 4 pools. Based on the results of this study, it is considered that continous surveillence of mosquitoes in livestock assembly facilities (slaughterhouse) should be performed for controlling mosquito populations and mediating disease spread by mosquitoes.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Animal Disease Management Technology Advancement Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Project No. 122013-2).

References

  1. CDC. 2021. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Vector-Borne Diseases. https://www.cdc.gov/ncezid/dvbd/about.html.
  2. Genc A, Eroglu F, Koltas IS. 2010. Detection of plasmodium vivax by nested PCR and real-time PCR. Korean J Parasitol 48: 99-103. https://doi.org/10.3347/kjp.2010.48.2.99
  3. Gleiser RM, Zalazar LP. 2010. Distribution of mosquitoes in relation to urban landscape characteristics. Bull Entomol Res 100: 153-158. https://doi.org/10.1017/S0007485309006919
  4. Korea Centers for disease control and prevention. 2021. Infectious disease management guidelines for vaccination in 2021. 11-1790387-000353-01: 183-184.
  5. Kutsuna S, Kato Y, Moi ML, Kotaki A, Ota M, Shinohara K, Kobayashi T, Yamamoto K, Fujiya Y, Mawatari M, Sato T, Kunimatsu J, Takeshita N, Hayakawa K, Kanagawa S, Takasaki T, Ohmagari N. 2015. Autochthonous dengue fever, Tokyo, Japan, 2014. Emerg Infect Dis 21: 517.
  6. Mangold KA, Reynolds SL. 2013. A revies of dengue fever: a resuring tropical disease. Pediatr Emerg Care 29: 665-669. https://doi.org/10.1097/PEC.0b013e31828ed30e
  7. Park GH, Kim SI, Cho SW, Cho SR, Lee SJ, Kim HK, Koo HN, Lee WG, Cho SH, Kim GH. 2018. Seasonal distribution of mosquitoes according to habitat environment (2016-2018). Korean J Appl Entomol 57: 381-392.
  8. Patel P, Landt O, Kaiser M, Faye O, Koppe T, Lass U, Sall AA, Niedrig M. 2013. Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses. Virol J 10: 58.
  9. Patz JA, Campbell-Lendrum D, Holloway T, Foley JA. 2005. Impact of regional climate change on human health. Nature 438: 310-317. https://doi.org/10.1038/nature04188
  10. Pearce JC, Learoyd TP, Langendorf BJ, Logan JG. 2018. Japanese encephalitis: the vectors, ecology and potential for expansion. J Travel Med 25: 16-26. https://doi.org/10.1016/j.tmaid.2018.05.014
  11. Quam MB, Sessions O, Kamaraj US, Rocklov J, Wilder-Smith A. 2016. Dissecting Japan's dengue outbreak in 2014. Am J Trop Med Hyg 94: 409.
  12. Reiter P. 2001. Climate change and mosquito-borne disease. Environ Health Perspect 109: 141-161. https://doi.org/10.1289/ehp.01109s1141
  13. Rhee SH, Kim TP, Lee TH, Kim MB, Yoon CH. 2018. Monitoring for prevalence of mosquitoes collected and pathogen infection. Korean J Vet Serv 41: 165-169.
  14. Succo T, Leparc-Goffart I, Ferre JB, Roiz D, Broche B, Maquart M, Noel H, Catelinois O, Entezam F, Caire D, Jourdain F, Esteve-Moussion I, Cochet A, Paupy C, Rousseau C, Paty MC, Golliot F. 2016. Autochthonous dengue outbreak in Nimes, South of France, July to September 2015. Euro Surveill 21: 30240.
  15. Wilson AJ, Morgan ER, Booth M, Norman R, Perkins SE, Hauffe HC, Mideo N, Antonovics J, McCallum H, Fenton A. 2017. What is a vector? Philos Trans R Soc Lond B Biol Sci 372: 20160085.
  16. Yang CF, Chen CF, Su CL, Teng HJ, Lu LC, Lin C, Wang CY, Shu PY, Huang JH, Wu HS. 2010. Screening of mosquitoes using SYBR Green I-based real-time RT-PCR with group-specific primers for detection of Flaviviruses and Alphaviruses in Taiwan. J Virol Methods 168: 147-151. https://doi.org/10.1016/j.jviromet.2010.05.006
  17. Yeom JS. 2017. Current status and outlook of mosquito-borne diseases in Korea. J Korean Med Assoc 60: 468-474. https://doi.org/10.5124/jkma.2017.60.6.468
  18. Yi H, Devkota BR, Yu JS, Oh KC, Kim J, Kim HJ. 2014. Effects of global warming on mosquitoes & mosquito-borne diseases and the new strategies for mosquito control. Entomol Res 44: 215-235. https://doi.org/10.1111/1748-5967.12084