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Fusarium Wilt Caused by Fusarium oxysporum on Passionfruit in Korea (Fusarium oxysporum에 의한 패션프루트 시들음병)

  • Joa, Jae-Ho;Choi, In-Young;Choi, Min-Kyung;Heo, Byong-Soo;Jang, Jong-Ok;Shin, Hyeon-Dong
    • Research in Plant Disease
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    • v.24 no.1
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    • pp.75-80
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    • 2018
  • From 2014 to 2016, Fusarium wilt disease was found on fassionfruit in Iksan and Jeju, Korea. Symptoms included wilting of foliage, drying and withering of leaves, and stunting of the plants. The infected plants eventually died during growth. Colonies on potato dextrose agar were pinkish white, and felted with cottony and aerial mycelia with 35 mm after one week. Macroconidia were falcate to almost straight, thin-walled and usually 2-3 septate. Microconidia were usually formed on monophialides of the hyphae and were hyaline, smooth, oval to ellipsoidal, aseptate or medianly 1-septate, very occasionally 2-septate, slightly constricted at the septa, $3-12{\times}2.5-6{\mu}m$. On the basis of the morphological characteristics and phylogenetic analyses of two molecular markers, internal transcribed spacer rDNA and translation elongation factor $1{\alpha}$, the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was proved by artificial inoculation, fulfilling Koch's postulates. To our knowledge, this is the first report on the occurrence of F. oxysporum on fassionfruit in Korea.

Measuring restraints stress responses using active biotelemetry in cattle (Active biotelemetry를 이용한 젖소의 보정 스트레스 반응 측정)

  • Lee, Dong-Hee;Lee, Byeong-Han;Lim, Joa-Jin;Kim, Jin-Young;Park, Hee-Myung;Chung, Byung-Hyun
    • Korean Journal of Veterinary Research
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    • v.42 no.2
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    • pp.277-282
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    • 2002
  • This study was conducted to investigate the influences of the rope and the tipping chute restraints on body temperature (BT) and heart rate (HR) as acute response for stress caused by restraining for diagnasis and treatment in cattle. Both parameters were recorded by active biotelemetry. In addition cortisol concentration in blood was analyzed as a indicator for stress response. Twelve cattle were divided into two groups based on hydraulic power, the rope restraint group and the tipping chute restraint group. BT and HR were measured at -30 (base), 0, 10, 20, 30, 40, 50, 60, 90, 120, and 180 minutes, including restraint period from 0 to 30 minutes during the experiment. The results obtained in this study was summarized as follows: 1. BT of the rope restraint group was increased ($39.8{\pm}0.3^{\circ}C$) until 20 min after restraint stress for 30 min, and then maintained with high values to the end of experiment. In the tipping chute restraint group, the BT was increased ($39.6{\pm}0.3^{\circ}C$) until the end of the restraint period, but then showed decrese until the end of experiment. 2. HR of both groups was maximized at the beginning of the restraint stress (P<0.05), and then it was decreased gradually but in the tipping chute restraint group showed increase again at the end of the reatraint stress (P<0.05). 3. The cortisol level of the rope restraint group was increased significantly ($9.72{\pm}5.09{\mu}g/d{\ell}$) until 30 min after the end of the restraint stress (P<0.05) and then decreased, but in the tipping chute restraint group showed great increase ($4.68{\pm}1.56{\mu}g/d{\ell}$) at the end of the restraint stress (P<0.05) and then decreased while the tipping chute restraint group was significantly lower than the rope restraint group 30 min after the restraint stress (P<0.05). In conclusion, this study suggests that the tipping chute restraint produces less response to physical stress than the rope restraint but the time for diagnasis and treatment should be shortened when using the tipping chute restraint.

Confidence Bounds following Adaptive Group Sequential Tests with Repeated Measures in Clinical Trials (반복측정자료를 가지는 적응적 집단축차검정에서의 신뢰구간 추정)

  • Joa, Sook Jung;Lee, Jae Won
    • The Korean Journal of Applied Statistics
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    • v.26 no.4
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    • pp.581-594
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    • 2013
  • A group sequential design can end a clinical trial early if a confirmed efficacy or a futility of study medication is found during clinical trials. Adaptation can adjust the design of clinical trials based on accumulated data. The key to this methodology is considered to control the overall type 1 error rate while maintaining the integrity of clinical trials. The estimation would be more complex and the sample size calculation will be more difficult if the clinical trials have repeated measurement data. Lee et al. (2002) suggested a repeated observation case by using the independent increments properties of the interim test statistics and investigated the properties of the proposed confidence interval based on the stage-wise ordering. This study extend Lee et al. (2002) to adaptive group sequential design. We suggest test statistics for the adaptation as redesigning the second stage of clinical trials and induce the stage-wise confidence interval of parameter of interests. The simulation will help to confirm the suggested method.

The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus

  • Chung, Bong Nam;Canto, Tomas;Tenllado, Francisco;Choi, Kyung San;Joa, Jae Ho;Ahn, Jeong Joon;Kim, Chun Hwan;Do, Ki Seck
    • The Plant Pathology Journal
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    • v.32 no.4
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    • pp.321-328
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    • 2016
  • We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of $10-20^{\circ}C$. However, infection rates subsequently decreased. Direct qRT-PCR of RNA extracted from a single aphid showed that PLRV infection increased in the $10-20^{\circ}C$ range, but this trend also declined shortly thereafter. We examined the effect of temperature on establishment of virus infection. The greatest number of plants became infected when N. benthamiana was held at $20^{\circ}C$ after inoculation with PVY-O or PVA. The largest number of P. floridana plants became infected with PLRV when the plants were maintained at $25^{\circ}C$. PLRV levels were highest in P. floridana kept at $20-25^{\circ}C$. These results indicate that the optimum temperatures for proliferation of PVY-O/PVA and PLRV differed. Western blot analysis showed that accumulations of PVY-O and PVA coat proteins (CPs) were lower at $10^{\circ}C$ or $15^{\circ}C$ than at $20^{\circ}C$ during early infection. However, accumulation increased over time. At $25^{\circ}C$ or $30^{\circ}C$, the CPs of both viruses accumulated during early infection but disappeared as time passed. Our results suggest that symptom attenuation and reduction of PVY-O and PVA CP accumulation at higher temperatures appear to be attributable to increased RNA silencing.

A Forecast Model for Estimating the Infection Risk of Bacterial Canker on Kiwifruit Leaves in Korea (참다래 잎에서의 궤양병 감염 위험도 모형)

  • Do, Ki Seok;Chung, Bong Nam;Joa, Jae Ho
    • Research in Plant Disease
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    • v.22 no.3
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    • pp.168-177
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    • 2016
  • A forecast model for estimating the infection risk of bacterial canker caused by Pseudomonas syringae pv. actinidiae on kiwifruit leaves in Korea was developed using the generic infection model of Magarey et al. (2005). Two-way contingency table analysis was carried out to evaluate accuracy of forecast models including the model developed in this study for estimating the infection of bacterial canker on kiwifruit using the weather and disease data collected from three kiwifruit orchards at Seogwipo in 2015. All the tested models had more than 80% of probability of detection indicating that all the tested models could be effective to manage the disease. The model developed in this study showed the highest values in proportion of correct (51.1%), probability of detection (90.9%), and critical success index (47.6%). It indicated that the model developed in this study would be the best model for estimating the infection of bacterial wilt on kiwifruit leaves in Korea. The model developed in this study could be used for a part of decision support system for managing bacterial wilt on kiwifruit leaves and help growers to reduce the loss caused by the disease in Korea.

Characteristics of Groundwater and Soil Contamination in Hallim Area of Jeju Island (제주도 한림지역의 지하수와 토양의 오염특성)

  • Hyun, Geun-Tag;Song, Sang-Tak;Joa, Dal-Hee;Ko, Yong-Hwan
    • Journal of Soil and Groundwater Environment
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    • v.15 no.3
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    • pp.44-51
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    • 2010
  • Contamination of groundwater from point and non-point sources is one of major problems of water resource manangement in Jeju island. This study characterized groundwater and soil contamination in Hallim area which is one of the areas of significantly contaminated soil and groundwater in Jeju Island. The amount of loaded contaminant (ALC) of Jeju area was estimated as 13,212 ton N/yr and 3,210 ton P/yr, The ALC of Hallim area was amounted to 2,895 ton N/yr and 1,102 ton P/yr, which accounted for 21.9% and 34.3% of the Jeju's ALC, respectively. The soil pH values (5.6-5.9) were not much different in land use areas. By contrat, average cation exchange capacity (CEC) of 14.1 $cmol^+/kg$ was high comparing to the nationwide range of 7.7-10.9 $cmol^+/kg$. Further, Sodium adsorption ratios (SARs) of horse ranch, pasture, and cultivating land for livestock were as high as 0.19, 0.17, and 0.16 respectively, comparing to the other landuse areas. Nitrate nitrogen at 22.2% of total groundwater wells exceeded 10 mg/L (the criteria of nitrate nitrogen for drinking water), averaginged 6.62 mg/L with maximum 28.95 mg/L. Groundwater types belonged to Mg-$HCO_3$, Na-$HCO_3$, Ca-$HCO_3$, and Na-Cl, among which Mg-$HCO_3$ type occupied more than 70% of the total samples, indicating the presence of anthropogenic sources. The concentration of nitrate nitrogen was negatively related to altitude and well depth, and positively related to the concentration of Ca, Mg, and $SO_4$ which might originate from chemical fertilizer. The ratio of nitrogen isotopes was estimated as an average of 8.10$^{\circ}/_{\circ\circ}$, and the maximum value of 17.9$^{\circ}/_{\circ\circ}$. According to the nitrogen isotope ratio, the most important nitrogen source was assessed as chemical fertilizer (52.6%) followed by sewage (26.3%) and livestock manures (21.1%).

Impact of Climate Change on Yield Loss Caused by Bacterial Canker on Kiwifruit in Korea (기후변화 시나리오에 따른 미래 참다래 궤양병 피해 예측)

  • Do, Ki Seok;Chung, Bong Nam;Choi, Kyung San;Ahn, Jeong Joon;Joa, Jae Ho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.2
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    • pp.65-73
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    • 2016
  • We estimated the averaged maximum incidences of bacterial canker at suitable sites for kiwifruit cultivation in 2020s and 2050s using D-PSA-K model with RCP4.5 and RCP8.5 climate change scenarios. Though there was a little difference between the estimation using RCP4.5 and that using RCP8.5, the estimated maximum disease incidences were more than 75% at all the suitable sites in Korea except for some southern coastal areas and Jeju island under the assumption that there are a plenty of infections to cause the symptoms. We also analyzed the intermediate and final outputs of D-PSA-K model to find out the trends on the change in disease incidence affected by climate change. Whereas increase of damage to kiwifruit canes in a non-frozen environment caused by bacterial canker was estimated at almost all the suitable sites in both the climate change scenarios, rate of necrosis increase caused by the bacterial canker pathogen in a frozen environment during the last overwintering season was predicted to be reduced at almost all the suitable sites in both the climate change scenarios. Directions of change in estimated maximum incidence varied with sites and scenarios. Whereas the maximum disease incidence at 3.14% of suitable sites for kiwifruit cultivation in 2020s under RCP4.5 scenario was estimated to increase by 10% or more in 2050s, the maximum disease incidence at 25.41% of the suitable sites under RCP8.5 scenario was estimated so.

Preference-Based Segment Buffer Replacement in Cluster VOD Servers (클러스터 VOD서버에서 선호도 기반 세그먼트 버퍼 대체 기법)

  • Seo, Dong-Mahn;Lee, Joa-Hyoung;Bang, Cheol-Seok;Lim, Dong-Sun;Jung, In-Bum;Kim, Yoon
    • Journal of KIISE:Computer Systems and Theory
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    • v.33 no.11
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    • pp.797-809
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    • 2006
  • To support the QoS streams for large scale clients, the internal resources of VOD servers should be utilized based on the characteristics of the streaming media service. Among the various resources in the server, the main memory is used for the buffer space to the media data loaded from the disks and the buffer hit ratio has a great impact upon the server performance. However, if the buffer data with high hit ratio are replaced for the new media data as a result of the number of clients and the required movie titles are increased, the negative impact on the scalability of server performance is occurred. To address this problem, the buffer replacement policy considers the intrinsic characteristics of the streaming media such as the sequential access to large volume data and the highly disproportionate preference to specific movies. In this paper, the preference-based segment buffer replacement policy is proposed in the cluster-based VOD server to exploit the characteristics of the streaming media. Since the proposed method reflects both the temporal locality by the clients' preference and the spatial locality by the sequential access to media data, the buffer hit ratio would be improved as compared to the existing buffer replacement policy. The enhanced buffer hit ratio causes the fact that the performance scalability of the cluster-based VOD server is linearly improved as the number of cluster nodes is increased.

On the Characteristics of Form Factors -Series 60, $C_B=0.60$- (Series 60, $C_B=0.60$ 선형의 형상계수의 특성고찰)

  • Kim, Hun-Chol;Yang, Seung-Il;Kim, Eun-Chan;Kang, Kuk-Jin;Van, Suak-Ho;Lee, Young-Gill;Kim, Yoon-Ho;Lee, Kwi-Joo;Kwak, Young-Ki;Joa, Soon-Won;Kim, Hyo-Chul;Kim, Wu-Joan;Song, Mu-Seok;Cho, Kyu-Jong;Hong, Sung-Wan;Lee, Seung-Hee;Sin, Yeong-Kiun
    • Bulletin of the Society of Naval Architects of Korea
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    • v.25 no.4
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    • pp.7-12
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    • 1988
  • The Resistance Committee of Korea Towing Tank Conference extended the Cooperative Experimental Study Program(1985)[1] to perform the geosim tests by exchanging the five different scaled Series 60, $C_B=0.60$ models between the participating organizations and 13 sets of resistance data have been obtained. The test results are compared among the participating towing tanks and also with the results given in the report of the Resistance and Flow Com-identify the mittee of 18th ITTC. The form factor of each model is derived by Prohaska's method to investigate its dependency on $R_n$. On the other hand, at each $F_n$, form factors are also derived by Telfer's method to relation between $F_n$ and form factor. For this hull form, form factors show relatively weak dependency on $R_n$ and strong dependency on $F_n$. And it is also found that dependencies on both have a cross relation. It seems that further study should be continued to understand more clearly the physical phenomena involved in this problem.

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Estimation of Duration of Low-temperature in Winter Season Using Minimum Air Temperature on January (1월 최저기온을 이용한 겨울철 저온발생일수 추정)

  • Moon, Kyung-Hwan;Son, In-Chang;Seo, Hyeong-Ho;Choi, Kyung-San;Joa, Jae-Ho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.3
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    • pp.119-123
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    • 2012
  • The duration of low temperature in winter season is one of the important agrometeorological characteristics in crop growing fields. This study was conducted to develop a method to estimate the duration of low-temperature with monthly meteorological data. Using daily meteorological data from 61 observation sites from 1981 to 2010, we analyzed the relationships between the averages of monthly temperature minima and the durations of low-temperature ranging from -15 to $5^{\circ}C$, The monthly mean of the January minimum air temperature was appropriate for theestimation of the durations of lowtemperature below $0^{\circ}C$. We tested a simple second order equation to predict durations of low-temperature. To apply the equation to various temperature ranges, we suggested two different equations for the estimation of coefficients a and b, which are dependent on the base temperatures from -15 to $0^{\circ}C$. Thevalidation of the equations using other daily meteorological datasets from 1971 to 2000 showed that they were appropriate for the range from -10 to $0^{\circ}C$, but underestimated at $-15^{\circ}C$.