The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus

  • Chung, Bong Nam (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Canto, Tomas (Centro de Investigaciones Biologicas, Centro de Investigaciones BiolOgicas-Spanish National Research Council) ;
  • Tenllado, Francisco (Centro de Investigaciones Biologicas, Centro de Investigaciones BiolOgicas-Spanish National Research Council) ;
  • Choi, Kyung San (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Joa, Jae Ho (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Ahn, Jeong Joon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Chun Hwan (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Do, Ki Seck (National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • Received : 2015.12.11
  • Accepted : 2016.03.15
  • Published : 2016.08.01
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Abstract

We examined the effects of temperature on acquisition of Potato virus Y-O (PVY-O), Potato virus A (PVA), and Potato leafroll virus (PLRV) by Myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. Infection by PVY-O/PVA and PLRV increased with increasing plant temperature in Nicotiana benthamiana and Physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of $10-20^{\circ}C$. However, infection rates subsequently decreased. Direct qRT-PCR of RNA extracted from a single aphid showed that PLRV infection increased in the $10-20^{\circ}C$ range, but this trend also declined shortly thereafter. We examined the effect of temperature on establishment of virus infection. The greatest number of plants became infected when N. benthamiana was held at $20^{\circ}C$ after inoculation with PVY-O or PVA. The largest number of P. floridana plants became infected with PLRV when the plants were maintained at $25^{\circ}C$. PLRV levels were highest in P. floridana kept at $20-25^{\circ}C$. These results indicate that the optimum temperatures for proliferation of PVY-O/PVA and PLRV differed. Western blot analysis showed that accumulations of PVY-O and PVA coat proteins (CPs) were lower at $10^{\circ}C$ or $15^{\circ}C$ than at $20^{\circ}C$ during early infection. However, accumulation increased over time. At $25^{\circ}C$ or $30^{\circ}C$, the CPs of both viruses accumulated during early infection but disappeared as time passed. Our results suggest that symptom attenuation and reduction of PVY-O and PVA CP accumulation at higher temperatures appear to be attributable to increased RNA silencing.

Keywords

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