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Prevalence of virulence-associated genes and antimicrobial resistance of Campylobacter jejuni from ducks in Gyeongnam Province, Korea

  • Yang, Jung-Wong;Kim, Sang-Hyun;Lee, Woo-Won;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.37 no.2
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    • pp.85-96
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    • 2014
  • Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck's fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were detected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was performed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kanamycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between $4.21{\pm}1.27{\times}10^4$ CFU/well and $1.053{\pm}0.451{\times}10^6$ CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between $1.00{\pm}1.73{\times}10^3$ CFU/well and $8.47{\pm}5.16{\times}10^4$ CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were $2.646{\pm}2.886{\times}10^5$ and $3.03{\pm}2.7{\times}10^4$ respectively, and that was $1.3230{\pm}1.2139%$ and $0.1516{\pm}0.1343%$ of the starting viable inoculum. There was considerable correlation ($R^2$=0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.

Studies on the Varietal Resistance of Rice to the Smaller Brown Planthopper, Laodelphax striatellus Fallen(IV) (애멸구에 대한 벼의 품종저항성에 관한 연구(VI))

  • Choi S. Y.;Song Y. H.;Park J. S.;Choi K. Y.
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.11-16
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    • 1974
  • Experiment was conducted to select the rice varieties and lines resistant to the smaller brown planthopper, Laodelphax striatellus Fallen, and study the cause of varietal resistance to the insect. The nature of varietal resistance was evaluated from the viewpoints of feeding and ovipositional preferences and antibiosis. Among the IRRI sources the varieties H 105, Muthumanikam, Vellailangalayan, Karsamba Red ASD-7. Manavari Co 22, Mudgo, PTB-18, IR 8 and IR 20 were selected as resistant sources. Among the domestic sources. only Suweon 213-1, Suweon 214 and Suweon 215 originated from the IR667 lines were resistant, and other leading varieties ail susceptible. The cause of resistance of rice to the insect seemed to be highly related with the non-feeding preference, not non-ovipositional preference. Nymphal mortality and rate of adult emergence were significantly different from the resistant and susceptible varieties. The rate of adult emergence was significantly lower in the resistant than In the susceptible varieties. The primary cause of lower adult emergence seemed to be due to the fact that the infects were suffered higher nymphal mortality in the resistant than in the susceptible varieties.

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The Effects of Father's Play Beliefs on Play Flow and Peer Play Behavior of Young Children, with Particular Focus on the Mediating Effects of Father's Play Participation (아버지의 놀이신념이 유아의 놀이몰입, 또래놀이행동에 미치는 영향:아버지의 놀이참여의 매개효과를 중심으로)

  • Kim, jeong Ju;Park, Hyoung Shin
    • Korean Journal of Child Education & Care
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    • v.17 no.3
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    • pp.29-48
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    • 2017
  • The purpose of this study was to analyze how father's play beliefs affect play flow and peer play behavior of young children, particularly as mediated by the father's play participation. For this purpose, 4 to 5-year-old children attending child care centers or kindergartens and their fathers were selected to participate in this study. Data on father's play beliefs and play participation were collected from the fathers, and data on the young children's play flow and peer play behavior from their teachers. Based on the collected data, the significance of the mediation model using Hayes's (2013) PROCESS macro was tested. According to the results of these analyses, the significant relationships between father's play beliefs and young children's play flow, reciprocal action of play and interruption of peer play were found mediated by father's play participation. This finding implies that father's play beliefs and his play participation may affect directly and indirectly the children's play flow and peer play behavior, underscoring the importance of father's play beliefs. In addition, the study's indicate that it is desirable to positively reinforce father's play beliefs by influencing father's play participation, in light of the likely positive influence of father's play beliefs over young children's social and emotional development.

Effects of Temperature and Mechanical Deformation on the Microhardness of Lead free and Composite Solders (무연 복합 솔더의 미소경도에 미치는 기계적 변형과 온도의 영향)

  • Lee Joo Won;Kang Sung K.;Lee Hyuck Mo
    • Journal of the Microelectronics and Packaging Society
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    • v.12 no.2 s.35
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    • pp.121-128
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    • 2005
  • Solder joints in microelectronic devices are frequently operated at an elevated temperature in service. They also experience plastic deformation caused by temperature excursion and difference in thermal expansion coefficients. Deformed solders can go through a recovery and recrystallization process at an elevated temperature, which would alter their microstructure and mechanical properties. In this study, to predict the changes in mechanical properties of Pb-free solder joints at high temperatures, the high temperature microhardness of several Pb-free and composite solders was measured as a function of temperature, deformation, and annealing condition. Solder alleys investigated include pure Sn, Sn-0.7Cu, Sn-3.5Ag, Sn-3.8Ag-0.7Cu, Sn-2.8Ag-7.0Cu (composite), and Sn-2.7Ag-4.9Cu-2.9Ni (composite). Numbers are all in wt.$\%$ unless specified otherwise. Solder pellets were cast at two cooling rates (0.4 and $7^{\circ}C$/s). The pellets were compressively deformed by $30\%$ and $50\%$ and annealed at $150^{\circ}C$ for 2 days. The microhardness was measured as a function of indentation temperature from 25 to $130^{\circ}C$. Their microstructure was also evaluated to correlate with the changes in microhardness.

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Design and Implementation of Security System Based on Intrusion Tolerance Technology : Focus on Wargame System (침입감내기술 기반의 보안시스템 설계 및 구현 : 워게임체계를 중심으로)

  • Lee, Gang-Tack;Lee, Dong-Hwi;J. Kim, Kui-Nam
    • Convergence Security Journal
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    • v.5 no.4
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    • pp.41-48
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    • 2005
  • Objective of this study is to design and implement security system based on intrusion tolerance technology for the improvement of dependability in defense system. In order to do so, I identify and extract core technologies through the research and analysis into characteristics, structures, main functions, and technologies of intrusion tolerance architecture. And I accomplish a design of security system through the redundant system based on these core technologies. To implement and verify intrusion tolerance system, I chose 'wargame system' as a subjected system, and accomplished 'Wargame Intrusion Tolerance System' and verified security required functions through a performance test. By applying showed security system into the development of application software based on intrusion tolerance, systematic and efficient system could be developed. Also applying 'WITDS' can solve the current security problems, and this will be basic model for design of security architecture in the federation system after.

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Applications of Panax ginseng leaves-mediated gold nanoparticles in cosmetics relation to antioxidant, moisture retention, and whitening effect on B16BL6 cells

  • Jimenez-Perez, Zuly Elizabeth;Singh, Priyanka;Kim, Yeon-Ju;Mathiyalagan, Ramya;Kim, Dong-Hyun;Lee, Myoung Hee;Yang, Deok Chun
    • Journal of Ginseng Research
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    • v.42 no.3
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    • pp.327-333
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    • 2018
  • Background: Bioactive compounds in plant extracts are able to reduce metal ions to nanoparticles through the process of green synthesis. Panax ginseng is an oriental medicinal herb and an adaptogen which has been historically used to cure various diseases. In addition, the P. ginseng leaves-mediated gold nanoparticles are the value-added novel materials. Its potential as a cosmetic ingredient is still unexplored. The aim of this study was to evaluate the antioxidant, moisture retention and whitening properties of gold nanoparticles (PgAuNPs) in cosmetic applications. Methods: Cell-free experiments were performed to evaluate PgAuNP's antioxidant and moisture retention properties and inhibition activity on mushroom tyrosinase. Furthermore, in vitro cell cytotoxicity was evaluated using normal human dermal fibroblast and murine B16BL6 melanoma cells (B16) after treatment with increasing concentrations of PgAuNPs for 24 h, 48 h, and 72 h. Finally, in vitro cell assays on B16 cells were performed to evaluate the whitening effect of PgAuNPs through reduction of cellular melanin content and tyrosinase activity. Results: In vitro DPPH radical scavenging assay results revealed that PgAuNPs exhibited antioxidant activity in a dose-dependent manner. PgAuNPs exhibited moisture retention capacity and effectively inhibited mushroom tyrosinase. In addition, 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide results revealed that PgAuNPs were not toxic to human dermal fibroblast and B16 cells; in addition, they significantly reduced melanin content, tyrosinase activity, and mRNA expression of melanogenesis-associated transcription factor and tyrosinase in B16 cells. Conclusion: Our study is the first report to provide evidence supporting that P. ginseng leaves-capped gold nanoparticles could be used as multifunctional ingredients in cosmetics.

Study on precision improvement in weight measurement of an egg for the automatic egg sorting system (계란(鷄卵) 자동(自動) 선별기(選別機)의 난중측정(卵重測定) 정밀도(精密度) 향상(向上)에 관(關)한 기초연구(基礎硏究))

  • Kim, K.D.;Bok, J.S.
    • Korean Journal of Agricultural Science
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    • v.27 no.1
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    • pp.48-53
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    • 2000
  • The various errors existing in a weight measurement system in most automatic egg sorting system available in Korean poultry farms have caused a large amount of economic losses to the egg producers. The object of this study was an importance of egg measurement system by changing both the number and the arrangements of load cells to reduce measuring errors. The results obtained were summarized as follow : 1. Four arrangements of load cells were selected as follows : layout I : Conventional one load cell method layout II : One load cell located as egg moving direction layout III : Two load cells located facing each other layout IV : Two load cells located as parallel with egg moving direction 2. The results of egg weight measurement according to four arrangements (Layout I, II, III, IV) showed that the average errors were 1.1218g, 0.5953g, 0.7786g, 0.2793g respectively. This indicated that the Layout IV (measuring by 2 load cells located parallel with the egg moving direction) caused the lowest average error and the best in precision. 3. The average vibration of axis X, y, Z were resulted as $5.1937{\times}10^{-3}G$, $9.3604{\times}10^{-3}G$, and $16.8657{\times}10^{-3}G$ respectively when sorting large sized egg. This indicated that the vibration of axis-Z was relatively higher than those of axis-X, and axis-Y.

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Evaluation of Tightness of Proximal tooth Contact on Implant Prostheses (임프란트 보철수복물에서의 인접치간 접촉강도의 평가)

  • Kim, Sang-Pil;Jung, J-Hyun;Kang, Dong-Wan;Oh, Sang-Ho
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.4
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    • pp.371-379
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    • 2008
  • The aim of this study was to evaluate the tightness of proximal tooth contact(TPTC) using a novel device at rest state on implant prostheses. Ten healthy young adults with class I normal occlusion consented to participate in the study and twenty patients were restored with a total 20 single-implant crowns in the left maxillary and mandibular second molars for 10 single-implant crowns, respectively. Test area were divided by 4 groups. UM describes the contact between the upper natural left first molar and natural second molar; LM the contact between the lower natural left first molar and natural second molar; IUM the contact between the upper natural left first molar and implant second molar and ILM the contact between the lower natural left first molar and implant second molar. The TPTC was measured at rest state in each area. The mean TPTC of the UM, LM, IUM and ILM was 1.48(${\pm}0.44$) N, 1.78(${\pm}0.40$) N, 1.14(${\pm}0.37$) N and 1.30(${\pm}0.32$) N respectively. These results indicate that the TPTC was less between natural tooth and implant prosthesis than between natural teeth.

Effects of Injection Configuration on Mixing in Supersonic Combustor

  • Sakamoto, Hayato;Matsuo, Akiko;Mitani, Tohru
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2004.03a
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    • pp.48-54
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    • 2004
  • The effects of injector spacing s and injector diameter d on mixing are numerically investigated in supersonic combustor with perpendicular injection behind a backward-facing step. Simulations are reported for airstream Mach number of 2.4. Parameters are changed on following 4 cases to investigate the effects of injector configuration on mixing efficiency $\eta_m$. In the case of varying d or s, dynamic pressure ratio $Rq(=(pu^2)_j/(pu^2)_a)$ is also varied to keep bulk equivalence ratio $\Phi({\oe})Rq.d^2/s)$ constant. (l) Injector spacing s is varied at constant $\Phi$=0.5, 1, 2 for injector diameter d=6mm. In the case of $\Phi$=1, $\eta_m$ has its maximum value at s=24mm. The reason is that increase of $\eta_m$. , by widening spacing at Rq=constant competes with decrease of $\eta_m$ by increasing Rq at s=constant. When spacing is narrow, the flow field of vicinity of injector becomes two-dimensional because adjacent jets interferes each other. By widening spacing, air is easily entrained by three-dimensional effect. This mechanism also appears in the case of $\Phi$=0.5, 2 for d=6mm, and $\eta_m$. reaches its maximum value at s=24mm for $\Phi$=0.5 and at s=42mm for $\Phi$=2. (2) In the case of injector diameter d varied at $\Phi$=1 for s=30mm, $\eta_m$. has its maximum value at d=3mm. The reason is that decrease of $\eta_m$ by increasing injector diameter competes with increase of $\eta_m$ by decreasing Rq at d=constant.(3) In the case of s varied at $\Phi$=0.5, 1,2 for d=3mm, the injector spacing at which mixing efficiency has its maximum value is s= 18mm for $\Phi$=0.5, s=24mm for $\Phi$=1, s=24mm for $\Phi$=2. Therefore it is found that d=3mm and s=24mm can be optimum configuration over a range of $\Phi$=0.5~2.(4) The effect of h on the optimum spacing is investigated. s is varied for d=6mm at step height h=4, 6, 8mm. The simulation results do not show significant change on the step height.

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Spectrometry Analysis of Fumes of Mixed Nuclear Fuel (U0.8Pu0.2)O2 Samples Heated up to 2,000℃ and Evaluation of Accidental Irradiation of Living Organisms by Plutonium as the Most Radiotoxic Fission Product of Mixed Nuclear Fuel

  • Kim, Dmitriy;Zhumagulova, Roza;Tazhigulova, Bibinur;Zharaspayeva, Gulzhanar;Azhiyeva, Galiya
    • Nuclear Engineering and Technology
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    • v.48 no.1
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    • pp.274-284
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    • 2016
  • Purpose: The purpose of this work is to describe the spectrometric analysis of gaseous cloud formation over reactor mixed uranium-and-plutonium (UP) fuel $(U_{0.8}Pu_{0.2})O_2$ samples heated to a temperature $>2,000^{\circ}C$, and thus forecast and evaluate radiation hazards threatening humans who cope with the consequences of any accident at a fission reactor loaded by UP mixed oxide $(U_{0.8}Pu_{0.2})O_2$, such as a mixture of 80% U and 20% Pu in weight. Materials and methods: The UP nuclear fuel samples were heated up to a temperature of over $2,000^{\circ}C$ in a suitable assembly (apparatus) at out-of-pile experiments' implementation, the experimental in-depth study of metabolism of active materials in living organisms by means of artificial irradiation of pigs by plutonium. Spectrometric measurements were carried out on the different exposed organs and tissues of pigs for the further estimation of human internal exposure by nuclear materials released from the core of a fission reactor fueled with UP mixed oxide. Results: The main results of the research described are the following: (1) following the research on the influence of mixed fuel fission products (radioactive isotopes being formed during reactor operation as a result of nuclear decay of elements included into the fuel composition) on living organisms, the authors determined the quantities of plutonium dioxide ($PuO_2$) that penetrated into blood and lay in the pulmonary region, liver, skeleton and other tissues; and (2) experiments confirmed that the output speed of plutonium out of the basic precipitation locations is very small. On the strength of the experimental evidence, the authors suggest that the biological output of plutonium can be disregarded in the process of evaluation of the internal irradiation doses.