• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.63-68
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• 2013

ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.11 no.2
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• pp.48-56
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• 2012

One kind of crowdsourcing techniques which allow drivers to collect and provide traffic information using smartphones and applications is being introduced to ITS system as well. The introduction of crowdsourcing techniques requires changes in the existing ITS based on Insourcing which provide traffic information generated by detectors through VMS. ITS Information have had two problems, one is the high cost and the other is an interrupted service. Experts expect crowdsourcing technique which is created SNS, will overcome problems of ITS. But, there are not many examples and research results. Crowdsourcing technique was utilized in Jeju ATMS project to install ITS on the coastal round roads around Jeju since ITS to install point detectors turned out to be non-economic method in case of the coastal round roads with low traffic volume. However, there existed links in which traffic information cannot be generated as there were no smartphone users (crowds) even in the cost-effective crowdsourcing techniques, which indicates the fact that the crowdsourcing method is suitable for urban roads with many smartphone users, but not for local minor roads. On the contrary, insourcing-based ITS is considered to be non-economic method in applying to all roads in the city, but it can be effectively utilized in the local minor roads. Accordingly, Inter-sourcing based ITS operating system in which insourcing is connected with crowdsourcing was suggested in this study.

• KIISE Transactions on Computing Practices
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• v.22 no.9
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• pp.426-440
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• 2016

Developed countries have already applied variable Intelligent Transportation System (ITS) technologies and have solved many transportation problems. Researchers also expect that ITS solutions can solve the transportation problems of Korean roads, when they are applied to Korean roads. However, since the existing ITS solutions applied in other countries are used for Korean roads without calibration, they will incur unexpected problems and high cost maintenance costs. In this paper, to solve the above problem, we propose the Korean Highway Traffic Analysis tool (KHTA). It not only analyzes all highway information in Korea, but also simulates the ITS algorithm implemented by traffic developers for the Korean highway environment. To test the stability, applicability and efficiency of the KHTA, we developed and analyzed the result of the ramp metering algorithm. The results show that the total travel time was reduced by 5 minutes compared to that without ramp metering, and traffic congestion was decreased. Thus, we confirmed that the KHTA can simulate ITS systems and can analyze the traffic environment in Korean highway. We expect that this tool would be very helpful to develop and analyze ITS systems in Korea.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.543-549
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• 2012

To gather the basic data for increasing the utilization of Isatis tinctoria, we examined the effects of both antioxidative enzyme activity and antimicrobial activity from the extract of Isatis tinctoria. Ascorbate Peroxidase activities reveal that there is an decrease in order; ethanol extract from its stem (1601.7 Unit/mg protein), methanol extract from its leaf (1133.7 Unit/mg protein) and distilled water extract from its leaf (524.3 Unit/mg protein). Catalase activities reveal that there is an decrease in order; ethanol extract from its flower petal (177.1 Unit/mg protein), methanol extract from its leaf (120.8 Unit/mg protein) and distilled water extract from its flower petal (55.4 Unit/mg protein). Peroxidase activities reveal that there is an decrease in order; ethanol extract from its flower petal (27.1 Unit/mg protein), methanol extract from its flower petal (14.6 Unit/mg protein) and distilled water extract from its stem (10.4 Unit/mg protein). Superoxide dismutase activities reveal that there is an increase in order; distilled water extract from its root (90.8%), methanol extract from its flower petal (80.1%) and ethanol extract from its root (75.5%). Its flower extract showed a antimicrobial activity only against Vibrio parahaemolyticus, its root extract had only against Staphylococcus aureus, and its stem extract had against Bacillus subtilis, Escherichia coli and Staphylococcus aureus, regardless of solvents. Especially, distilled water extract from its leaf showed a high antimicrobial activity against both Bacillus subtilis and Escherichia coli and inhibition diameters against those were 30.0 and 24.0 mm, respectively.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.96-104
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• 2012

Phylogenetic analyses were conducted to evaluate relationships of 7 taxa of Korean Carpesium including three outgroup (Inula britannica L., Inula germanica L., Rhanteriopsis lannginosa (DC.) Rauschert) by using ITS (internal transcribed spacer) sequences of nuclear ribosomal DNA. Phylogenetic studies used maximum parsimony, neighbor-joining and maximum likelihood methods analysis. The length of the ITS sequences was 731 bp, and the lengths of the ITS1, ITS2 and 5.8S regions were 284~297 bp, 264~266 bp and 164 bp, respectively. The total number of variable sites was 111 for the entire sequences, and a parsimony informative sites of 64 are valid. Base change appeared variously in ITS1 rather than in ITS2. As the result, Korean Carpesium were formed monophyletic group and C. abrotanoides situated as the most basal clade. The results show that C. macrocephalum is closely related with C. triste. C. rosulatum has the closest relationship with C. glossophyllum. C. cernuum is close to C. divaricatum. These results suggest that the ITS data used in this study could be useful for the phylogenetic analysis of Korean Carpesium.

• The Journal of the Korea Contents Association
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• v.22 no.10
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• pp.526-534
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• 2022

In August 2014, the Ministry of Land, Infrastructure, and Transport (MOLIT) devised an innovative ITS measure in which private and public sectors share roles to maximize investment efficiency and effectiveness in collecting and offering traffic information that had been separately implemented by the state and private sector. The main details of the innovative measure include the following: For communication information, the information collected by the private sector is used, and the state concentrates on safety-related information collection, such as unexpected situations, including construction, accidents, and deteriorating weather conditions. Consequently, safety-related information is offered in real-time through smartphones and navigation, in addition to electric road signs that have limitations in providing unexpected real-time situations due to installation at specific spots. This study presented a connected traffic information priority coordination plan to improve the accuracy of traffic information offering by analyzing problems of related traffic information, including a general national highway case study to enhance the efficiency of national highway ITS implementation, according to actual public-private traffic information sharing. In addition, this study reviewed whether to operate or demolish the information collection equipment by analyzing traffic volume level and availability of related traffic information in the existing ITS operation sections and presented ITS collection equipment installation judgment standards based on the cases concerned.

• Journal of Life Science
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• v.14 no.6 s.67
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• pp.963-966
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• 2004

The sequence of ITS (partial 16S ribosomal DNA, complete ITS1, 5.8S ribosomal DNA and ITS2, and partial 28S ribosomal DNA) was analysed by PCR and autosequencing in Sarcodon aspratus. The ITS lenght of S. aspratus was 716 base pair. As this sequence compared with other reports on S. aspratus (ace No AF335110), the sequence variation based on nucleotide deletion and substitution was $1.8\%$. This nucleotide variation rate in same species was very higher than in other species. Also, the sequence varitation rates between this S. aspratus and S. imbricatus, and S. squamus were $8\%\;and\;10\%$, respectively. This results suggested that the high sequence variation of S. aspratus might be caused specific host and inhabitat environment which limited gene flow.

• Journal of Navigation and Port Research
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• v.31 no.6
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• pp.491-496
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• 2007

Recently ITs(Information Technologies} have been as essential methods for port operation. However, despite of the importance of ITs, there has not been any research that evaluate and analyze current level of ITs performance and their utilizations. Thus, objective of this study is to develop integrated indicators to evaluate current level of ITs performance and their utilizations in container terminal. To confirm the effectiveness of the suggested indicators, current level of ITs performance in a container terminal was measured, and then some implications based on findings were presented.

• International Journal of Highway Engineering
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• v.16 no.6
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• pp.19-25
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• 2014

PURPOSES : This study was performed to evaluate the possibility of Indirect Tensile Strength (ITS) as a testing method that can predict cracking on pavement. METHODS : Three asphalt binders and one kind of aggregate were used in this study, and all asphalt mixtures were produced using Gyratory Compactor followed asphalt mix design. The ITS test was performed for the mixture which are artificially short-term aged using the oven. The ITS properties were analyzed by air void, compaction temperature, asphalt content, and asphalt binder. RESULTS : The results of this study indicated that (1) the compaction temperature did not show relationship with the ITS test; (2) there was no specific trend between the asphalt content and the ITS test; (3) the ITS could reveal the property of kinds of asphalt binders; (4) the asphalt mixture that were produced at optimum temperature suggested by manufacturer did not exhibit optimum result for all asphalt binder. CONCLUSIONS : The possibility of ITS was confirmed from this study for replacement of the Marshall Stability method. However, it needs to perform in further studies of aggregate and compaction property to suggest a new ITS standard value.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.816-821
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• 2004

A reliable molecular phylogenetic method to identify Hericium erinaceum, the most industrially valuable species in the Hericium genus, was established. Sequencing and phylogenetic analyses of the PCR-amplified ITS and 5.8S rDNA from Hericium fungi, including 6 species and 23 isolates, showed that variation in nucleotide sequences and size exists in both ITS1 and ITS2 regions, but not in the 5.8S region. These two ITS regions provided different levels of information on the relationship of H. erinaceum to other Hericium species. Based on the ITS1 sequence, both the parsimony and neighbor joining trees clearly distinguished Asian H. erinaceum isolates from other Hericium species and isolates. The intraspecific divergence of the ITS2 region was suitable to dissect the Asian H. erinaceum isolates into a few groups.