Browse > Article

Molecular Identification of Asian Isolates of Medicinal Mushroom Hericium erinaceum by Phylogenetic Analysis of Nuclear ITS rDNA  

Park, Hyuk-Gu (School of Life Sciences and Biotechnology, Korea University)
Ko, Han-Gyu (School of Life Sciences and Biotechnology, Korea University)
Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Science, Dankook University)
Park, Won-Mok (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Journal of Microbiology and Biotechnology / v.14, no.4, 2004 , pp. 816-821 More about this Journal
Abstract
A reliable molecular phylogenetic method to identify Hericium erinaceum, the most industrially valuable species in the Hericium genus, was established. Sequencing and phylogenetic analyses of the PCR-amplified ITS and 5.8S rDNA from Hericium fungi, including 6 species and 23 isolates, showed that variation in nucleotide sequences and size exists in both ITS1 and ITS2 regions, but not in the 5.8S region. These two ITS regions provided different levels of information on the relationship of H. erinaceum to other Hericium species. Based on the ITS1 sequence, both the parsimony and neighbor joining trees clearly distinguished Asian H. erinaceum isolates from other Hericium species and isolates. The intraspecific divergence of the ITS2 region was suitable to dissect the Asian H. erinaceum isolates into a few groups.
Keywords
Hericium erinaceum; medicinal mushroom; nuclear ribosomal DNA; ITS1-5.8S-ITS2; intraspecific variation; phylogenetic identification;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
연도 인용수 순위
1 Ampliication and diect sequencing of fungal ribosomal RNA genes for phylogenetics /
[ White, T. J.;T. Bruns;S. Lee;J. Taylor;M. A. Innis(ed.);D. H. Gelfand(ed.);J. J. Sninsky(ed.);T. J. White(ed.) ] / PCR Protocols: A Guide to Methods and Applications
2 ITS2 is a double-edged tool for eukaryotic evolutionary comparisons /
[ Coleman, A. W. ] / Trends Genet   DOI   ScienceOn
3 /
[ Arora, D. ] / Mushrooms Demystified
4 Hericium coralloides and Hericium alpestre basidiomycetes /
[ Hallenberg, N. ] / Mycotaxon
5 Isolation and characteristics of Tichoderma harzianum FJ1 producing cellulases and xylanase /
[ Kim, K. C.;S.-S.Yoo;Y.-A. Oh;S. J. Kim ] / J. Microbiol. Biotechnol.
6 Survey in medicinal value of Hericium erinaceus /
[ Liu, M.;H. Cheng;H. Sun ] / Edible Fungi of China
7 Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR /
[ Kim, S. H.;A. Uzunovic;C. Breuil ] / Appl. Environ. Microbiol.
8 Phylogeographic divergences of nuclear ITS sequences in Coprinus species sensu lato /
[ Ko, K. S.;Y. M. Lim;Y. H. Kim;H. S. Jung ] / Mycol. Res.   DOI   ScienceOn
9 Development of rapid molecular detection marker for Colletrotrichum spp. in leaf and fruit tissues of sweet persimmon /
[ Lee, S.-P.;Y.-S.Lee ] / J. Microbiol. Biotechnol.
10 PCR-based sensitive detection of medicinal fungi Hericium species from ribosomal internal transcribed spacer (ITS) sequences /
[ Lu, L.;J. Li;Y. Cang ] / Biol. Pharm. Bull.   DOI   ScienceOn
11 Bioactive substances in Hericium erinaceus (Bull.; fr.) Per. (Yamabushitake), and its medicinal utilization /
[ Mizuno, D. ] / Int. J. Med. Mush   DOI
12 Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, and edible and medicinal mushroom called yamabushitake or houtou /
[ Mizuno, T.;T. Wasa;H. Ito;C. Suzuki;N. Ukai ] / Biosci. Biotech. Biochem.   DOI   ScienceOn
13 Sequence or structure? A short review on the application of nucleic acid sequence information to fungal taxonomy /
[ Mitchell, J. I.;P. J. Roberts;S. T. Moss ] / Mycologist   DOI   ScienceOn
14 Secondary structure and phylogenetic implications of ITS2 in the genus Tricholoma /
[ Suh, S. J.;J. G. Kim ] / J. Microbiol. Biotechnol.
15 /
[ Swofford, D. L. ] / PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods)
16 Hypolipideic effect of exo-polymer produced in submerged mycelial culture of five diferent mushrooms /
[ Yang, B. K.;J. B. Park;C. H. Song ] / J. Microbiol. Biotechnol.
17 The evolutionary biology and population genetics underlying fungal strain typing /
[ Taylor, J. W.;D. M. Geiser;A. Burt;V. Koufopanou ] / Clin. Microbiol. Rev.
18 CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice /
[ Thompson, J. D.;D. G. Higgins;T. J. Gibson ] / Nucleic Acids Res.   DOI   ScienceOn
19 Antitumor and immunoenhancing activities of polysaccharide from broth of Hericium spp. /
[ Wang, J.-C.;S.-H. Hu;C.-H. Su;T.-M. Lee ] / Kaohsiung J. Med. Sci.