• Biomedical Science Letters
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• v.20 no.3
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• pp.180-184
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• 2014

Mitochondria play a crucial role in many essential biological events by way of the electron transport chains and intermembrane proteins that they contain. Abnormalities in the mitochondria are strongly correlated with the development of diseases such as atherosclerosis, cancer, and diabetes. However, the study of mitochondria has been referred to as 'labor-intensive' because of the difficulty in isolating the organelles from their various sources, which can include cultured cells and tissues. Multiple companies provide mitochondria isolation kits, and it is possible for investigators to use different kits and apply different protocols depending on the source of the mitochondria. Therefore, we focused on producing an isolation buffer that could be applied to both cultured cells and tissues, and optimized an isolation protocol that could be used with both. Specifically, we adjusted the buffer condition that can be applied to human cervical cancer cells, fibroblasts, and tissues such as mouse liver and spleen. We also optimized the protocol to improve the efficacy and efficiency of the steps involved in the isolation of mitochondria. These methodological improvements may contribute to advanced research by allowing investigators to overcome the difficulties involved in isolation of mitochondria from biological samples.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.65 no.6
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• pp.1032-1037
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• 2016

In this paper, the method of isolation enhancement for the mobile phone MIMO antenna of LTE class 40(2300~2400MHz) was studied. Design of MIMO antenna was based on the hybrid antenna that operates both a monopole and an IFA(Inverted F Antenna). A structure for the isolation enhancement which controls induced electric field on the ground plane is located between MIMO antenna, and was not connected with the ground but apart 0.3mm. A MIMO antenna that operates on class 3~40(1710~2400MHz) of LTE service bands and a structure for the isolation enhancement at the class 10 band were designed. VSWR measurement of implemented antenna on the FR4 board showed within 3:1 at entire design bands, and isolation between antennas at the class 40 band was less than -30dB. Isolation was enhanced more than 20dB by the studied structure. ECC(Envelope Correlation Coefficient) for MIMO performance was under 0.1, and antenna average gain and efficiency measured in the anechoic chamber were -4.28~-1.40dBi and 37.32~72.36% respectively.

• Smart Structures and Systems
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• v.8 no.5
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• pp.501-524
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• 2011

Recent studies have discovered that a conventional passive isolation system may suffer from an excessive isolator displacement when subjected to a near-fault earthquake that usually has a long-period velocity pulse waveform. Semi-active isolation using variable friction dampers (VFD), which requires a suitable control law, may provide a solution to this problem. To control the VFD in a semi-active isolation system more efficiently, this paper investigates experimentally the possible use of a control law whose control logic is similar to that of the anti-lock braking systems (ABS) widely used in the automobile industry. This ABS-type controller has the advantages of being simple and easily implemented, because it only requires the measurement of the isolation-layer velocity and does not require system modeling for gain design. Most importantly, it does not interfere with the isolation period, which usually decides the isolation efficiency. In order to verify its feasibility and effectiveness, the ABS-type controller was implemented on a variable-friction isolation system whose slip force is regulated by an embedded piezoelectric actuator, and a seismic simulation test was conducted for this isolation system. The experimental results demonstrate that, as compared to a passive isolation system with various levels of added damping, the semi-active isolation system using the ABS-type controller has the better overall performance when both the far-field and the near-fault earthquakes with different PGA levels are considered.

• Smart Structures and Systems
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• v.26 no.2
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• pp.195-211
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• 2020

Among anti-seismic technologies, base isolation is a very effective means of mitigating damage to structural and nonstructural components, such as equipment. However, most seismic isolation systems are designed for mitigating only horizontal seismic responses because the realization of a vertical isolation system (VIS) is difficult. The difficulty is primarily due to conflicting isolation stiffness demands in the static and dynamic states for a VIS, which requires sufficient rigidity to support the self-weight of the isolated object in the static state, but sufficient flexibility to lengthen the isolation period and uncouple the ground motion in the dynamic state. To overcome this problem, a semi-active VIS, called the piezoelectric inertia-type vertical isolation system (PIVIS), is proposed in this study. PIVIS is composed of a piezoelectric friction damper (PFD) and a leverage mechanism with a counterweight. The counterweight provides an uplifting force in the static state and an extra inertial force in the dynamic state; therefore, the effective vertical stiffness of PIVIS is higher in the static state and lower in the dynamic state. The PFD provides a controllable friction force for PIVIS to further prevent its excessive displacement. For experimental verification, a shaking table test was conducted on a prototype PIVIS controlled by a simple controller. The experimental results well agree with the theoretical results. To further investigate the isolation performance of PIVIS, the seismic responses of PIVIS were simulated numerically by considering 14 vertical ground motions with different characteristics. The responses of PIVIS were compared with those of a traditional VIS and a passive system (PIVIS without control). The numerical results demonstrate that compared with the traditional and passive systems, PIVIS can effectively suppress isolation displacement in all kinds of earthquake with various peak ground accelerations and frequency content while maintaining its isolation efficiency. The proposed system is particularly effective for near-fault earthquakes with long-period components, for which it prevents resonant-like motion.

• Journal of Korean Association for Spatial Structures
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• v.18 no.4
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• pp.81-88
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• 2018

Seismic isolation systems have typically been used in the form of base seams in mid-rise and low-rise buildings. In the case of high-rise buildings, it is difficult to apply the base isolation. In this study, the seismic response was analyzed by changing the installation position of the seismic isolation device in 3D high - rise model. To do this, we used 30-story and 40-story 3D buildings as example structures. Historic earthquakes such as Mexico (1985), Northridge (1994) and Rome Frieta (1989) were applied as earthquake loads. The installation position of the isolation device was changed from floor to floor to floor. The maximum deformation of the seismic isolation system was analyzed and the maximum interlaminar strain and maximum absolute acceleration were analyzed by comparing the LB model with seismic isolation device and the Fixed model, which is the base model without seismic isolation device. If an isolation device is installed on the lower layer, it is most effective in response reduction, but since the structure may become unstable, it is effective to apply it to an effective high-level part. Therefore, engineers must consider both structural efficiency and safety when designing a mid-level isolation system for high-rise buildings.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1993.04a
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• pp.147-147
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• 1993

All types of dynamic excitation, periodical, pulse or transient in vertical, horizontal or all three directions can be effectively reduced by vibration isolation systems. Typical elements for vibration isolation control are spring units consisting of a group of helical compression springs. In all cases of shock, transient or random excitation energy absorbing dampers have to be added to the spring units in order to reduce system response in the frequency range near the natural frequency of the isolation system. The same isolation system of spring units and viscos-dampers has been used since 1979 for passive protection of buildings and structures has been proved to by very advantageous for vibration and structure borne noise control. Not only because of high vertical flexibility of the spring units, compared for example with typical rubber or neoprene mounts out also because of the horizontal of flexibility, which can be adapted by modifying the spring dimensions to nearly every requirement. It is just normal to use the same basic elements for passive isolation as for active isolation.

• Journal of the Optical Society of Korea
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• v.17 no.1
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• pp.103-108
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• 2013

The operation of a Doppler wind LIDAR in a mobile environment is very sensitive to shocks and vibrations, which can cause critical failures such as misalignment of the optical path and damage to optical components. To be able to stabilize the LIDAR and to perform wind field measurements in motion, a shock absorption and vibration isolation system was designed and implemented. The performance of the vehicle-mounted Doppler wind LIDAR was tested in motion, first in a circular test route with a diameter of about 30 m and later in regular expressway traffic. The vibration isolation efficiency of the system was found to be higher than 82% in the main vibration area and shock dynamic deflection was smaller than maximal deflection of the isolator. The stability of the laser locking frequency in the same mobile environment before and after the vibration isolation system installation was also found to be greatly improved. The reliability of the vibration isolation system was confirmed by good results of the analysis of the LIDAR data, in particular the plane position indicator of the line of sight velocity and the wind profile.

• Smart Structures and Systems
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• v.24 no.3
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• pp.345-360
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• 2019

The shear keys are set in a seismic isolation system to resist the long-term service loadings, and are cut off to isolate the earthquakes. This paper investigated the influence of shear keys on the seismic performance of a vertical spring-viscous damper-concave Coulomb friction isolation system by an incremental dynamic analysis (IDA) and a performance-based assessment. Results show that the cutting off process of shear keys should be simulated in a numerical analysis to accurately predict the seismic responses of isolation system. Ignoring the cutting off process of shear keys usually leads to untrue seismic responses in a numerical analysis, and many of them are unsafe for the design of isolated structure. And those errors will be increased by increasing the cutting off force of shear keys and decreasing the spring constant of shear keys, especially under a feeble earthquake. The viscous damping action postpones the cutting off time of shear keys during earthquakes, and reduces the seismic isolation efficiency. However, this point can be improved by increasing the spring constant of shear keys.

• Journal of Sensor Science and Technology
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• v.30 no.2
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• pp.119-124
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• 2021

Isolation and separation of biological nanoparticles, such as cells and extracellular vesicles, are important techniques for their characterization. Dielectrophoresis (DEP) based on microfluidic chips is an effective method to isolate and separate the nanoparticles. However, the electrodes of the DEP chips are electrolyzed by the electrical signals applied to the nanoparticles. Thus, the isolation/separation efficiency of the nanoparticles is reduced considerably. Through this study, we developed a microfluidic DEP chip for reliable isolation/ separation of nanoparticles and developed a passivation technique for the protection of the DEP chip electrodes. The electrode passivation process was designed using a hydrogel and the stability of the hydrogel passivation layer was verified. The fabricated DEP chip and the proposed passivation technique were used for the collection and dispersion of the fluorescent polystyrene nanoparticles. The proposed chip and the technique for isolation and separation of nanoparticles can be leveraged in various bioelectronic applications.

• Journal of Microbiology and Biotechnology
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• v.19 no.7
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• pp.647-650
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• 2009

Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter isolates were obtained from 178 samples (89%) by direct streaking of colon mucosa, 142 samples (71%) by direct streaking of a swab, and 94 samples (47%) by pre-enrichment of intestinal contents in Preston broth. Direct streaking of colon mucosa was superior to the other two isolation methods, in terms of rapidity and higher efficiency. When isolates were identified with various biochemical tests and PCRs specific to 16s rRNA, mapA, and ceuE, C. coli was the predominant species (87%) in porcine, whereas the rest of the isolates were identified as C. lanienae.