Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Development of dielectrophoresis chips and an electrode passivation technique for isolation/separation of nanoparticles

나노 입자 분리/분류를 위한 유전영동 칩 및 전극 패시베이션 기술 개발

  • Park, Minsu (School of Electronic and Electrical Engineering, Kyungpook National University) ;
  • Noh, Hyowoong (School of Electronic and Electrical Engineering, Kyungpook National University) ;
  • Kang, Jaewoon (School of Electronic and Electrical Engineering, Kyungpook National University) ;
  • Lee, Junyeong (School of Electronic and Electrical Engineering, Kyungpook National University) ;
  • Park, Hongsik (School of Electronic and Electrical Engineering, Kyungpook National University)
  • 박민수 (경북대학교 대학원 전자전기공학부) ;
  • 노효웅 (경북대학교 대학원 전자전기공학부) ;
  • 강재운 (경북대학교 대학원 전자전기공학부) ;
  • 이준영 (경북대학교 대학원 전자전기공학부) ;
  • 박홍식 (경북대학교 대학원 전자전기공학부)
  • Received : 2021.03.19
  • Accepted : 2021.03.30
  • Published : 2021.03.31
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Abstract

Isolation and separation of biological nanoparticles, such as cells and extracellular vesicles, are important techniques for their characterization. Dielectrophoresis (DEP) based on microfluidic chips is an effective method to isolate and separate the nanoparticles. However, the electrodes of the DEP chips are electrolyzed by the electrical signals applied to the nanoparticles. Thus, the isolation/separation efficiency of the nanoparticles is reduced considerably. Through this study, we developed a microfluidic DEP chip for reliable isolation/ separation of nanoparticles and developed a passivation technique for the protection of the DEP chip electrodes. The electrode passivation process was designed using a hydrogel and the stability of the hydrogel passivation layer was verified. The fabricated DEP chip and the proposed passivation technique were used for the collection and dispersion of the fluorescent polystyrene nanoparticles. The proposed chip and the technique for isolation and separation of nanoparticles can be leveraged in various bioelectronic applications.

Keywords

References

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