• Journal of Biomedical Engineering Research
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• v.44 no.6
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• pp.465-475
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• 2023

In this study, we demonstrated a silica nanofibrous membrane based on the electrospinning process and evaluated its DNA isolation and purification performance in PCR pretreatment. Generally, silica membranes made of non-woven fabric are used for PCR pretreatment, but this study aimed to improve the efficiency of the pretreatment process by developing a nanofiber-type silica membrane with high specific surface area and porosity. In order to manufacture a nanofiber-shaped silica film while maintaining the original physical properties of silica, nanofiber membranes produced by adding various concentrations of PEO (5 wt%, 8 wt%, and 10 wt%) to silica prepared by the sol-gel method were compared. In terms of nanofiber membrane production, the higher the PEO concentration, the more effective it was in producing nanofiber membranes. The produced silica nanofiber membrane was inserted to a pretreatment device used in commercial PCR equipment, and the pretreatment performance was compared and verified using Salmonella bacteria. When Salmonella was used, samples containing 5 wt% PEO showed superior PCR efficiency compared to samples containing 8 wt% and 10 wt% PEO. These results show that adding 5 wt% of PEO can effectively improve DNA purification and separation by producing a nanofiber-shaped silica film while maintaining the physical properties of silica. We expect that this study will contribute to the development of effective PCR pretreatment technology essential for various molecular biology applications.

• Journal of the Semiconductor & Display Technology
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• v.23 no.1
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• pp.12-18
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• 2024

The accelerated pace of climate crisis due to continuous industrialization and greenhouse gas emissions necessitates sustainable solutions that simultaneously address mitigation and adaptation to climate change. Naturebased Solutions (NbS) have gained prominence as viable approaches, with Green Infrastructure being a representative NbS. Green Infrastructure involves securing green spaces within urban areas, providing diverse climate adaptation functions such as removal of various air pollutants, carbon sequestration, and isolation. The proliferation of Green Infrastructure is influenced by the quantification of improvement effects related to various projects. To support decision-making by assessing the climate vulnerability of Green Infrastructure, the U.S. Department of Agriculture (USDA) has developed i-Tree Tools. This study proposes a comprehensive evaluation approach for climate change adaptation types by quantifying the climate adaptation performance of urban Green Infrastructure. Using i-Tree Canopy, the analysis focuses on five urban green spaces covering more than 30 hectares, considering the tree ratio relative to the total area. The evaluation encompasses aspects of thermal environment, aquatic environment, and atmospheric environment to assess the overall eco-friendliness in terms of climate change adaptation. The results indicate that an increase in the tree ratio correlates with improved eco-friendliness in terms of thermal, aquatic, and atmospheric environments. In particular, it is necessary to prioritize consideration of the water environment sector in order to realize climate change adaptive green infrastructure, such as increasing green space in urban areas, as it has been confirmed that four out of five target sites are specialized in improving the water environment.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.189-195
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• 2014

Basic leucine zipper (bZIP) protein is a regulatory transcription factor that plays crucial roles in growth, development and stress response of plant. In this study, we isolated a PagbZIP1 gene that belonged to Group SE3 of bZIP from Populus alba ${\times}$ P. glandulosa, and investigated its expressional characteristics. The PagbZIP1 is 844 base pairs long and encodes a putative 144-amino-acid protein with an expected molecular mass of 16.6 kDa. The PagbZIP1 has two conserved domains including the basic and leucine zipper portions. Southern blot analysis revealed that two copies of the gene are presented in the poplar genome. PagbZIP1 was specifically expressed in the root and suspension cells. Moreover, the expression of PagbZIP1 was induced by drought, salt, cold and ABA. Therefore, our results indicated that PagbZIP1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in poplar.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.425-432
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• 2017

The purpose of this study is to analyze mission statements of social welfare centers. For this, we collected mission statements of 385 social welfare centers nationwide. We analyzed data using the NVivo 10 program. Analysis results found the total number of collected words was 1,401. The number of words corresponding to the target of organizations was 448 (32.0%), the number of words corresponding to problems was 51 (3.6%), the number of words corresponding to the directionality 118 (8.4%), the number of words corresponding to strategy was 545 (38.9%), and the number of words corresponding to organization's image was 239 (17.1%). Second, the words of the intervention target were resident of community, local community and community. Intervention issues included community problems, poverty, and social isolation. The words of the organizational directivity included welfare community/village, social welfare, and empowerment. Words related to organizational strategy were formation/building, sharing/serving, and improvement/development/improvement. Words related to the organizational image included in the mission statements were the welfare center, we, the professional agency, and the neighbors. By analyzing the mission statements, we found that social welfare centers portrayed its identity based on 'locality'. The distribution of the words related to the community was high in both the target, target, and direction. The limitations of this study include the exclusion of analyzing the relationship between organizational mission and organizational performance. This should be considered in future studies.

• Journal of Plant Biotechnology
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• v.41 no.4
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• pp.194-200
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• 2014

Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba ${\times}$ P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.

• Korean Educational Research Journal
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• v.42 no.1
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• pp.35-72
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• 2021

This study is to form develops an effective teaching method centered on the Korean "symbol adverb + predicate" type, helping Chinese students to learn Korean to communicate more accurately when expressing detailed complex feelings and various emotions.Manyforeignlanguage learners try to memorize individual words when they acquire the new vocabulary, this may lead to a problematic in that they cannot use Korean vocabulary accurately and naturally because they do not value the combination of vocabulary words. Since symbolic adverbs are not used in isolation and being frequently used with certain vocabulary words, it is more effective to teach them in the form of instruct learners using "symbol adverb + predicate" forms rather than individual vocabulary words. Accordingly, this research considers a particular vocabulary following symbolic adverbs or vocabulary groups with common semantic qualities that could be frequently introduced. Seven Korean language textbooks used by university in domestic Korea and China are compared and analyzed to reveal the aspects of differences in the use of descriptive words after symbolic adverbs. Finally, based on the textbook analysis results, the government propose a plan to improve the Korean "symbol adverb + predicate" type for Chinese learners. However, this study was limit to being unable to present specific educational measures for Chinese learners in the form of "symbol adverb + predicate". This is expected to complement the limitations of this study in subsequent studies, and lead to more specific discussions.

• The Journal of the Korea Contents Association
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• v.22 no.4
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• pp.535-546
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• 2022

This study tried to explore factors for improving academic achievement in online non-face-to-face education based on the survey results of a University's online cooperative learning Q&A. Due to the Corona situation, both professors and learners can easily feel psychological isolation due to the implementation of all non-face-to-face online classes. As one of the methods, it was intended to suggest the direction of future education to various teachers and learners by sharing class cases in which the online cooperative learning methodology was applied. Previous studies on non-face-to-face online learning, online cooperative learning, and learning promotion method were reviewed, and the online Q&A method was adopted as a specific learning promotion method to conduct research. In the Q&A process, learners were given an opportunity to check their learning content, share knowledge and communicate, and performance evaluation-related factors such as guaranteeing anonymity of the questioner and answerer, improvement points system, and absolute evaluation were asked. As a result of the survey analysis, it was found that they are the success factors of online cooperative learning. It is a small change that can be applied in practice in the future where online non-face-to-face learning is likely to continue, but by sharing meaningful cases of application of teaching methodologies, both professors and learners being motivated and actively involved in. It is expected that we will be able to suggest methods and directions for improving skills together by changing and supplementing the learning field.

• Korean Chemical Engineering Research
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• v.47 no.2
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• pp.220-229
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• 2009

Strain improvement and morphology investigation in bioreactor cultures were undertaken in suspended cultures of Phellinus linteus mycelia for mass production of protein-bound polysaccharides(soluble ${\beta}$-D-glucan), a powerful immuno-stimulating agent. Phellineus sp. screened for this research was identified as Phellinus linteues through ITS rDNA sequencing method and blast search, demonstrating 99.7% similarity to other Phellinus linteus strains. Intensive strain improvement program was carried out by obtaining large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because large numbers of protoplasts ($1{\times}10^5{\sim}10^6\;protoplasts/ml$) formed using the banding filtration method with the cell wall-disrupting enzymes could be regenerated in relatively high regeneration frequency($10^{-2}{\sim}10^{-3}$) in the newly developed regeneration medium. It was demonstrated that the strains showing high performances in the protoplast regeneration and solid growth medium were able to produce 5.8~6.4%(w/w) of ${\beta}$-D-glucan and 13~15 g/L of biomass in stable manners in suspended shake-flask cultures of P. linteus mycelia. In addition, cell mass increase was observed to be the most important in order to enhance ${\beta}$-D-glucan productivity during the course of strain improvement program, since the amount of ${\beta}$-D-glucan extracted from the cell wall of P. linteus mycelia was almost constant on the unit biomass basis. Therefore we fully investigated the fungal cell morphology, generally known as one of the key factors affecting cell growth extent in the bioreactor cultures of mycelial fungal cells. It was found that, in order to obtain as high cell mass as possible in the final production bioreactor cultures, the producing cells should be proliferated in condensed filamentous forms in the growth cultures, and optimum amounts of these filamentous cells should be transferred as active inoculums to the production bioreactor. In this case, ideal morphologies consisting of compacted pellets less than 0.5mm in diameter were successfully induced in the production cultures, resulting in shorter period of lag phase, 1.5 fold higher specific cell growth rate and 3.3 fold increase in the final biomass production as compared to the parallel bioreactor cultures of different morphological forms. It was concluded that not only the high-yielding but also the good morphological characteristics led to the significantly higher biomass production and ${\beta}$-D-glucan productivity in the final production cultures.

• KSBB Journal
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• v.25 no.2
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• pp.155-166
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• 2010

Studies on the production of cell-wall bound innerpolysaccharides (IPS) (soluble ${\beta}$-D-glucan) have been performed by use of suspended myelial cultures of Inonotus obliquus. This product has promising potentials as an effective antidiabetic as well as an immunostimulating agents. As a first step to enhanced production of IPS, Intensive strain improvement programs were carried out by obtaining a large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because about fivefold higher amount of protoplasts ($2.3{\times}10^6$ protoplasts/mL) could be recovered with relatively high regeneration rates of $10^{-2}{\sim}10^{-3}$ by applying a modified filtration method, as compared to the previously used trapping method. A basic protocol necessary for UV-mutation of the protoplasts was also developed, resulting in several overproducing variants with good fermentation properties. Since the amount of IPS extracted from the mycelial cell walls of I. obliquus turned out to be almost constant per g DCW, increase in cell mass was considered the most important factor for the enhancement in IPS production. Therefore, attempts were made to screen mutant cells showing rapid mycelial growth rate in the final suspended cultures. Notably, the mutant strains showing an active cellgrowth in the preceding solid growth cultures were observed to produce higher amount of IPS in the suspended fermentations as well. A striking mutant, OBLQ756-15-5 strain, obtained from the survivors of a harsh UV-treated condition (97% death rate) was found to stably produce as high cell mass as 22 g DCW/L in the final fermentations. Currently, this strain is being tested for development of a scaled-up fermentation process for mass production of IPS.

• The Korean Journal of Mycology
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• v.25 no.2 s.81
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• pp.143-151
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• 1997

Interspecific hybrids between Aspergillus niger and Penicillium notatum (Tyr-), hyperlipolytic enzyme-producing fungi, were obtained by nuclear transfer technique. Optimal conditions for formation of intergeneric hybrids were investigated. Maximum production of protoplasts was obtained by 1% Novozyme 234 at $30^{\circ}C$ for 3 hrs and the most effective osmotic stabilizers for the isolation of protoplasts were 0.6 M KCl. Frequencies of hybrid formation by nuclear transfer were $3.8{\times}10^{-3}{\sim}1.3{\times}10^{-3}$. From the observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyotypes are aneuploid. The hybrids showed $1.2{\sim}1.7$ fold higher lipase activities than parental strains. It was strongly supported by results of this study that nuclear transfer technique is much more efficient in the formation of intergeneric hybrids than protoplast fusion and is very useful for the improvement of strains.