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http://dx.doi.org/10.14578/jkfs.2014.103.2.189

Isolation and Characterization of a Basic Leucine Zipper Gene in Poplar (Populus alba × P. glandulosa)  

Yoon, Seo-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute)
Lee, Hyoshin (Division of Forest Tree Improvement, Korea Forest Research Institute)
Bae, Eun-Kyung (Division of Forest Tree Improvement, Korea Forest Research Institute)
Choi, Young-Im (Division of Forest Biotechnology, Korea Forest Research Institute)
Kim, Joon-Hyeok (Division of Forest Biotechnology, Korea Forest Research Institute)
Noh, Seol Ah (Division of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.103, no.2, 2014 , pp. 189-195 More about this Journal
Abstract
Basic leucine zipper (bZIP) protein is a regulatory transcription factor that plays crucial roles in growth, development and stress response of plant. In this study, we isolated a PagbZIP1 gene that belonged to Group SE3 of bZIP from Populus alba ${\times}$ P. glandulosa, and investigated its expressional characteristics. The PagbZIP1 is 844 base pairs long and encodes a putative 144-amino-acid protein with an expected molecular mass of 16.6 kDa. The PagbZIP1 has two conserved domains including the basic and leucine zipper portions. Southern blot analysis revealed that two copies of the gene are presented in the poplar genome. PagbZIP1 was specifically expressed in the root and suspension cells. Moreover, the expression of PagbZIP1 was induced by drought, salt, cold and ABA. Therefore, our results indicated that PagbZIP1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in poplar.
Keywords
ABA; abiotic stress; PagbZIP1; Populus alba ${\times}$ P. glandulosa; transcription factor;
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