• Title/Summary/Keyword: Isolated starch

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Screening of Giberella sp. from the Korean Paddy Field for the roduction of Gibberellic Acid and its Cultural Properties (Gibberellic acid를 생산하는 분리주 Gibberella sp. 의 배양학적 특성)

  • 오영준
    • Microbiology and Biotechnology Letters
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    • v.23 no.2
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    • pp.119-122
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    • 1995
  • A different form from Gibberella fujikuroi was isolated from the paddy field of Naju area. The strain, designated as Y107, was identified as Gibberrella sp. based on its morphological, physiological, and biochemical characteristics. The highest production of Gibberellic acid by the strain was achieved in a fermentation medium containing corn starch, glucose, soybean oil, soybean meal, NH$_{4}$NO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$, and trace elements.

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Isolation and Quantitative Determination of Allatoin from Dioscoreae Rhizoma (산약(山藥)(Dioscoreae Rhizoma)으로부터 Allantoin의 분리 및 함량분석)

  • Hwang Gwi-Seo
    • Journal of Society of Preventive Korean Medicine
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    • v.7 no.1
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    • pp.133-138
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    • 2003
  • It is well known that Dioscoreae Rhizoma tonifies and benefits the Spleen and Stomach, benefits the Lung and nourishes the Kidney. A major component isolated from this herb consist of dioscin, diosgenin, allantoin, starch, alanin, mucilage, choline, amylase, and glycoprotein. In this study, we aimed to measure the content of allantoin, one of major component, to determine the quality of Dioscoreae Rhizoma.

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Production of Protease from Thermophilic Actinomyces (고온성 방선균이 생산하는 단백질 분해효소의 생산)

  • 김중배
    • The Korean Journal of Food And Nutrition
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    • v.13 no.2
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    • pp.171-175
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    • 2000
  • Microbial proteases have certain unique characteristics, and are now widely used in food, leather, detergent, and pharmaceutical industries. Thermophilic Actinomyces producing the protease was isolated from soil in Wonju city. This strain was able to grow and produce protease at the culture temperature of 50$^{\circ}C$. The maximum protease production was obtained when 0.5% soluble starch and 0.4% yeast extract were used as carbon and nitrogen source, respectively. The other culture condition for the maximal productivity of the protease was 0.1% K2HPO4, and 0.05% CaCl2 at initial pH 8.0 for 48 hours.

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Composition and Partial Structure Characterization of Tremella Polysaccharides

  • Khondkar, Proma
    • Mycobiology
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    • v.37 no.4
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    • pp.286-294
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    • 2009
  • Heteropolysaccharides isolated from liquid cultures of nine Tremella species contained 0.3 to 1.2% protein, 2.7 to 5% ash, 0.9 to 3.4% acetyl groups, 76.5 to 84.2% carbohydrates and trace amounts of starch. The polysaccharides in aqueous solution were slightly acidic (pH 5.1 to 5.6). They consisted of the following monomeric sugars: fucose, ribose, xylose, arabinose, mannose, galactose, glucose and glucuronic acid. The backbones of the polysaccharide structures consisted of $\alpha$-(1$\rightarrow$3)-links while the side chains were $\beta$-linked.

Physicochemical and Organoleptic Properties of Starch Isolated from Gamma-Irradiated Acorn (감마선 조사 도토리로부터 분리한 전분의 이화학적 및 관능적 특성)

  • Kwon, Joong-Ho;Kim, Soo-Jin;Lee, Jung-Eun;Lee, Soo-Jeong;Kim, Sung-Kon;Kim, Jeong-Sook;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1007-1012
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    • 2002
  • Physicochemical and organoleptic properties were investigated in starch extracted from acorn gamma-irradiated for insect control. Hunter's color L, a, and b values were unchanged upon irradiation at 0.25 to 10 kGy. Scanning electron microscopic observation revealed no changes with gamma irradiation at 1 kGy, that is effective for disinifestation, whereas 10 kGy resulted in some clefts on the starch surface. X-ray diffraction analysis showed patterns of both amorphous and crystalline regions were not different among the treatment groups. Water-binding property, swelling power, solubility, and gelatinization patterns of starch were influenced by irradiation dose, but 1 kGy dose was not detrimental to the physicochemical properties. Textural parameters of acorn gel were relatively stable, but significant reductions were found in hardness, adhesiveness, and chewiness in samples irradiated at 3 kGy or higher. Rrsults revealed that irradiation at 1 kGy or lower could be applied for insect control without causing apparent changes in physicochemical and organoleptic properties of acorn starch.

Production of Bleach-Stable and Halo-Tolerant Alkaline Protease by an Alkalophilic Bacillus pumilus JB05 Isolated from Cement Industry Effluents

  • Johnvesly, B.;Naik, Gajanan R.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.558-563
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    • 2001
  • A new alkalophilic strain of Bacillus pumilus JB¬05 producing bleach-stable and halo-tolerant alkaline protease was isolated from cement industry effluents in Karnataka, India. The effects of carbon and nitrogen sources on protease production by this alkalophilic strain were observed after a 30-h incubation. A high level of alkaline protease activity was obtained in the presence of starch as the carbon and peptone as the nitrogen sources. The partially purified enzyme showed an optimum temperature and pH activity at $58^{\circ}C$ and 10.5, respectively. The enzyme was completely inhibited by PMSF (95.0%) indicating it as a serine protease. It is bleach-stable as it retained 35% original activity in the presence of 10% (v/v) hydrogen peroxide at $30^{\circ}$C after 2 h and is halo-tolerant as it retained 70% original activity in the presence of 2.5 M sodium chloride at $30^{\circ}C$ after 2 h incubation.

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Mariannaea samuelsii Isolated from a Bark Beetle-Infested Elm Tree in Korea

  • Tang, Longqing;Hyun, Min-Woo;Yun, Yeo-Hong;Suh, Dong-Yeon;Kim, Seong-Hwan;Sung, Gi-Ho;Choi, Hyung-Kyoon
    • Mycobiology
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    • v.40 no.2
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    • pp.94-99
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    • 2012
  • During an investigation of fungi from an elm tree infested with bark beetles in Korea, one isolate, DUCC401, was isolated from elm wood. Based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer and 28S rDNA (large subunit) sequences, the isolate, DUCC401, was identified as Mariannaea samuelsii. Mycelia of the fungus grew faster on malt extract agar than on potato dextrose agar and oatmeal agar media. Temperature and pH for optimal growth of fungal mycelia were 25oC and pH 7.0, respectively. The fungus demonstrated the capacity to degrade cellobiose, starch, and xylan. This is the first report on isolation of Mariannaea samuelsii in Korea.

Isolation of Exopolysaccharide-Producing Bacillus polymyxa KS-1 and Some Properties of Exopolysaccharide (다당류를 생산하는 Bacillus polymyxa KS-1의 분리 및 생산 다당류의 특성)

  • 권기석;주현규;오태광
    • Microbiology and Biotechnology Letters
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    • v.20 no.1
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    • pp.34-39
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    • 1992
  • For the screening of new-functional and specific exopolysaccharide, a bacterium strain was isolated from soil through the two steps of screening. The isolated bacterium was identified as Bacillus polymyxa KS-1 according to the criteria of morphological, physiological, and chemical taxonomic analyses. The exopolysaccharide was composed of glucose:galactose: mannose and galactosamine in an approximate molar ratio of 1.00:0.36:1.02:1.10. The produced exopolysaccharide by Bacillus polymyxa KS-1 was found to be revealed new acidic polysaccharide which did not contain pentose, ketose, starch, and uronic acid.

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천일염으로부터 고호염균의 분리 및 동정

  • 박형숙;정명주
    • Microbiology and Biotechnology Letters
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    • v.24 no.6
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    • pp.671-677
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    • 1996
  • An Extremely halophilic bacterium was isolated from solar salts. The isolated strain was Gram-negative, facultatively anaerobic, and motile bacterium. The colony was circular, smooth, and red-orange color. The strain showed pleomorphism depending on magnesium ion concentrations. The range of temperature and pH for growth of the isolate were 35 -45$\circ$C and 7.0 - 9.0. NaCl concentration for growth of it was 4.3 - 5.0 M. The isolate was catalase and oxidase positive, and sensitive to bacitracin. It showed starch hydrolyzing and acid forming characteristics. DNA G+C content was 62.7 mol%. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Haloarcula vallismortis, therefore it was identified as Haloarcula sp. EH-1.

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Chemical Components and Physicochemical Properties of Precipitates in Red Ginseng Extracts Isolated by Centrifugation (홍삼 Ext중 원심력에 의하여 분리된 침전물의 화학조성과 물리화학적 특성)

  • 김나미;양재원
    • Journal of Ginseng Research
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    • v.18 no.2
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    • pp.122-127
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    • 1994
  • Red ginseng extracts accounted for the major precipitating material in red ginseng drink prepared with various components and pasteuralized at $80^{\circ}C$ for 30 min. Precipitates in red ginseng extracts isolated by centrifugation were investigated for its chemical components and physicochemical properties. Recovery of precipitates in red ginseng extracts was 1.59% on a dry weight basis. Precipitates were composed of starch (71.47%), crude protein (21.75%), pectin (1.70%), polyphenol (2.97%) and calcium (3.83 ppm) but ginsenosides were not present. Absorption peak at 285 nm which appeared in red ginseng extracts was absent in the precipitates. pH and Hunter L value of the precipitates decreased, while Hunter a and b values increased in contrast to red ginseng extracts. Precipitates were solubilized to some extent in polar solvent. The shape of precipitate particle was round, sciral or irregular square. Particle size of precipitates varied from 0.17 $\mu\textrm{m}$ to 41.3 $\mu\textrm{m}$ in length.

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