천일염으로부터 고호염균의 분리 및 동정

  • Published : 1996.12.01

Abstract

An Extremely halophilic bacterium was isolated from solar salts. The isolated strain was Gram-negative, facultatively anaerobic, and motile bacterium. The colony was circular, smooth, and red-orange color. The strain showed pleomorphism depending on magnesium ion concentrations. The range of temperature and pH for growth of the isolate were 35 -45$\circ$C and 7.0 - 9.0. NaCl concentration for growth of it was 4.3 - 5.0 M. The isolate was catalase and oxidase positive, and sensitive to bacitracin. It showed starch hydrolyzing and acid forming characteristics. DNA G+C content was 62.7 mol%. The morphological, physiological and biochemical characteristics of the isolate resembled those of the Haloarcula vallismortis, therefore it was identified as Haloarcula sp. EH-1.

Keywords

References

  1. Microbial Ecol. v.3 The microbial ecology of the Great salt lake Post,F.J.
  2. Appl. Microbiol. v.2 The reddening of salted hides and fish Aderson,H.
  3. Can. J. Res. v.10 Bacteriological studies on the red discoloration of salted hides Lochhead,A.G.
  4. Bergery's manual of systematic bacteriology v.3 Extremely halophilic archaebacteria. Order Halobacteriales ord. nov. Grant,W.D.;H.Larsen;N.Pfennig(ed.)
  5. Microbiology of Extreme Environments Halotolerant and halophilic microorganism Gilmour,D.;C.Edward(sed.)
  6. The Bacteria v.8 The halobacteriaceae Kushner,D.J
  7. Syst. Appl. Microbiol. v.5 Natronobacterium gen. nov. and Natronococcus gen. nov., two new genera of haloalkaliphilic archaebacteria Tendall,B.N.J.;H.N.M.Ross;W.D.Grant
  8. System. Appl. Microbiol. v.8 Classification of non-alkaliphilic halobacteria based on numerieal taxonomy and polar lipid composition, and description of Haloarecula gen. nov. and Haloferax gen. nov Torreblanca,M.;B.Rodriguez-Valera;G.Juez;A.Ventosa;M.Kamekura;M.Kates
  9. J. Gen. Appl. Microbiol. v.41 Taxonomy of the family halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba Kamekura,M.;M.L.Dyall-Smith
  10. Can. J. Microbiol. v.6 Effect of some metal ions growth of Halobacterium cutirubrum Sehgal,S.N.;N.E.Gibbons
  11. Can. J. Microbiol. v.15 Growth and nutrition of extremely halphilic bacteria Gochnauer,M.B.;D.J.Kushner
  12. J. Gen. Microbiol. v.55 Proteolytic enzymes from extremely halophilic bacteria Norberg,P.;B.V.Hofsten
  13. Appl. Environ. Microbiol. v.51 Accumulation of Poly(β-Hydroxybutyrate) by Halobacteria Fernandez-castillo,R.;F.Rodriguez-valera.;J.Gonzalezramos;F.Ruiz-berraquero
  14. The Prokaryotes The family Halobacteriaceae Tindall,BJ.;Balows,A.(ed.);Truper,H.G.(ed.);Dworkin,M.(ed.);Harder,W.(ed.);Schleier,K.H.(ed.)
  15. Can. J. Microbiol. v.18 Halobacterium saccharovorum sp. nov., a carbohydrate-metabolizing. extremely halophilic bacterium Tomlinson,G.A.;L.I.Hochstein
  16. Methods in microbiology v.3B Isolations, growth and requirements of halophilic bacteria Gibbons,N.E.;Norris(ed.);Gibbons(ed.)
  17. Can. J. Microbiol. v.24 Halobacterium vallismortis sp. nov., An amyloytic and carbohydrate-metabolizing, extremely halophilic bacterium Gonzalez,C.;C.Gutierrez;C.Ramirez
  18. Manual of methods for general bacteriology General characterization Smibert,R.M.;N.R.Krieg
  19. J. Bacteriol. v.70 An improved technique for staining halophilic bacteria Dussault,H.P.
  20. Microbiology, a laboratory manual(2nd ed.) Biochemical activities of microorganisms Cappuccino,J.G.;N.Sherman
  21. Can. J. Microbiol. v.18 Studies on acid production during carbohydrate metabolism by extremely halophilic bacteria Tomlinson,G.A.;L.I.Hochstein
  22. FEMS Microbiol. Letters. v.25 Determination of DNA base composition by reversed-phase high performance liquid chromatohraphy Tamaoka,J.;K.Komagata
  23. 미생물과 산업 v.21 호염성 및 내염성 미생물 이홍금
  24. J. Gen. Microbiol. v.139 Archaeal halophiles (halobacteria) from two British saltmines Norton,C.F.;T.J.McGenity;W.D.Grant
  25. J. Bacteriol. v.151 Boxshaped halophilic bacteria Javor,B.;C.Requadt;W.Stoeckenius
  26. Advances in microbial physiology v.15 Physiology of Halobacteriaceae Dundas,I.E.D.
  27. Can. J. Microbiol. v.1 The effect of magnesium, potassium and iron on the growth and morphology of red halophilic bacteria Brown,H.J.;N.E.Gibbons
  28. Can. J. Microbiol. v.18 Isolation of carbohydrate-metabolizing, extremely halophilic bacteria Tomlinson,G.A.;L.I.Hochstein
  29. Advabces in microbiol physiology v.1 Biochemical aspects of extreme halophilism Larsen,H.
  30. Korean J. Life Science v.6 Microbial DNA base composition (G+C mol%) and its taxonomic implications Shi,Y.K.;J.S.Lee;H.J.Kim;W.H.Joo;J.D.Lee;Y.K.Park