• Environmental Engineering Research
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• 제23권1호
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• pp.62-69
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• 2018

In the present study, long-term heavy metals (HMs) contaminated soil samples from a well-known Pb/Zn smelting area in the southwest of China were collected, and physicochemical and biological characteristics of these samples were evaluated. Soil samples contained different concentrations of HMs, namely Pb, Zn, Cu, and Cd. Enzyme activity analyses combined with microcalorimetric analysis were used for soil microbial activity evaluation. Results showed that two soil samples, containing almost the highest concentrations of HMs, also shared the greatest microbial activities. Based on correlation coefficient analysis, high microbial activity in heavily HMs contaminated soil might be due to the high contents of soil organic matter and available phosphorus in these samples. High-throughput sequencing technique was used for microbial community structure analysis. High abundance of genera Sphingomonas and Thiobacillus were also observed in these two heavily contaminated soils, suggesting that bacteria belonging to these two genera might be further isolated from these contaminated soils and applied for future studies of HMs remediation. Results of present study would contribute to the evaluation of microbial communities and isolation of microbial resources to remediate HMs pollution.

• 대한간호학회지
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• 제48권3호
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• pp.349-361
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• 2018

Purpose: This ethnography was performed to explore patterns and meanings of healthy life among aged women using senior centers. Methods: The informants were 21 individuals aged 65 years and older at 2 community-based senior centers. Data were collected from iterative fieldwork through in-depth interviews and participant observations and analyzed using text analysis and taxonomic methods developed by Spradley. Field notes were used with follow-up interviews and dialogue between authors to enhance interpretation. Results: Patterns of healthy life among aged women using senior centers were categorized by age groups within the context of the four cultural elements of taking care of the body, relationality, temporality, and spatiality: active and passive control, maintenance of interdependence and individuality, expansion and maintenance of the daily routine, unity of peer relations and sustenance of family relations, spending time productively and tediously, and complementary and alternative space of the family relations. Conclusion: The informants in this study demonstrated healthy life by maintaining and strengthening continuous relationships developed in the senior centers without being isolated from the family and society. Patterns of their healthy life differed across age groups within the socio-cultural context. Therefore, interventions should be tailored to address age groups and community needs.

• Journal of Microbiology
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• 제38권1호
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• pp.11-17
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• 2000

A new method was developed for the rapid analysis of diverse bacterial species in the natural environment. Our method is based on PCR-single-strands-conformation polymorphism (PCR-SSCP) and selective isolation technique of single-stranded DNA. Variable V3 fragments of 16S rDNA were amplified by PCR with bacterial 16S rDNA primers, where one of the primers was biotinylated at the 5'-end. The biotinylated strands of the PCR products were selectively isolated by using streptavidin paramagnetic particles and a magnetic stand, to prevent SSCP analysis producing heteroduplexes from heterogeneous DNA samples. The selected strands were separated by electrophoresis on a polyacrylamide gel, and detected by silver staining. Analysis of PCR products from 8 bacterial strains demonstrated their characteristic DNA band patterns. In addition, changes in the structure of the bacterial community and species diversity in the microcosm treated with phenol could be monitored. After 3 weeks of incubation, phenol and its intermediate, 2-hydroxy-muconic-semialdehyde, were degraded by indigenous bacteria. These dominating bacterial populations were identified as strong bands on an SSCP gel. Therefore, this study provides useful tools for microbial community analysis of natural habitats.

• 농촌의학ㆍ지역보건
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• 제17권2호
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• pp.123-127
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• 1992

Of 80 cases of Salmonellosis reported in a provincial area in 1992, 90% were related to outbreaks, which occurred from Apr. to Nov. And the serotypes include the following: typhimurium, hadar, and enteritidis etc. Among these outbreaks the authors carried out the epidemiological investigation for 'I-eup'(highest attack region) outbreak. The results obtained were as follows: 1. The S. typhimurium isolated from stools of 7 cases among 29 cases. 2. The common-source of exposure was boiled pork and/or skewered slices of seasoned meat. 3. The incidence rate was 65.1%(29 cases). 4. The incidence showed significant difference between males(32.1%) and females(80.0%). 5. The incidence in under 20 years of age and 40 years of age were much higher than other age groups but there was no significant difference among much age groups. 6. The mean and the median incubation period were 18.5 and 17.0 hours respectively. 7. The secondary attack was not appeared.

• 한국보건간호학회지
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• 제35권3호
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• pp.401-414
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• 2021

Purpose: The purpose of this study was to compare the practice of quarantine guidelines and psychological influence between the young (aged under 64 years) and the elderly (aged over 65 years) groups during the coronavirus disease 19 (COVID-19) pandemic. Method: The data of this study were obtained from the Community Health Survey, 2020, Korean Disease Control and Prevention Agency. A total of 229,296 (young group: 156,314, elderly group: 72,812) participants were included in this study. Independent t-test, chi-square test and descriptive analysis were used for analyzing the data. Result: Elderly group showed lower adherence to wearing face-mask, maintaining social distance when outgoing and practicing handwashing rules than the young group. The elderly group showed a higher level of psychological influence than the young group. In addition, the elderly group had fewer supporters when they were isolated than the young group. Also, the elderly had difficulties in following the quarantine guidelines related to COVID-19. Conclusion: To prevent infection and death from COVID-19 in the elderly, proper education and support programs are needed. Also, support groups should be prepared and applied to relieve their psychological impact related to COVID-19.

• 영어영문학
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• 제57권2호
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• pp.237-264
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• 2011

Based on its geophysical feature, the island has long been recognized as a separate and self-sustaining space independent of neighboring continent or other islands. Literary tradition has used the island as a metaphor for a utopian alternative to mundane human society with its various kinds of wrongdoings. Recent nature writings have taken up this island metaphor to emphasize the wholeness of the ecosystem in specifically designated natural community or landscapes such as national parks or wilderness preservation areas. Human-nature relations as border-divided area is also recognized as the island. Modern island biogeography, however, has disproved such a concept of islands as autonomous, revealing the contrasting fact that the richness of species on an undisturbed island is determined largely by species immigration from and emigration to a source of colonists. This scientific finding has posited the island as the interconnected nature, but the public and metaphoric use of it still resorts to the old concept of it as isolated and autonomous nature, because this image has been ingrained deeply in our consciousness and culture. Considering the negative consequences from the recognition of nature and nature-humans as isolated space, we need a new nature metaphor that embodies interconnectedness in nature and of human-nature relations. Such feature of interconnectedness is best embedded in the concept of ecotone. Some ecotones are created and maintained through human participation in nature, and this human induced nature of ecotone denotes the possibilities of a constructive relation between them. The substitution of the island with the ecotone as the concept of nature and the image of human-nature relations is expected to correct ecocritical practices of reading of nature writing, which has been predominantly interpreted within the orientation of nature itself and nature-human relations as an isolated and self-autonomous island. Adopting the ecotone in literary study enables ecocriticism to dig out cultural elements embedded in nature writing and reveal socio-political, ideological factors hidden behind the writers' portrayal of nature as islands.

• 미술이론과 현장
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• 제3호
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• pp.35-50
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• 2005

For us, any regional art exist? Almost all art events today take place mostly in Seoul. Concentrating on the reinforcement of its power, the regional art world remains isolated without any connection with the Seoul art community. It is completely closed off from the central art scene. The regional art world seems to consider that more helpful in consolidating its status. It is in real sense suffering the absence of art criticism and art media, lack of an understanding of art and backwardness of art education. Many regional artists are dreaming of moving out from their domain, aspiring to be a member of the central art world. They make an effort to assimilate into a refined, modern style of the central art circle, rather than striving to create works imbued with regionality and locality. As the artists living in the provinces, the characteristics of their district should be above all else reflected in their art. Although they are less informed about the latest trend of culture and art, their art has to be something passionately and obstinately embodying the intrinsic quality of their regions. They have to find a practical solution more positively, instead of merely complaining about the poor condition of the regional art community. There is the need to bring about a turnabout in our awareness that regional art is confined to any limit and cannot be in the center of the entire art world. What' s most significant is to dissolve factionalism and not to depend on school ties or regional networks. As a reaction against authoritarianism, regional art has to put emphasis on attaining its originality. For this, regional art should respect regional tradition, history and background and break away from an old convention of wielding power.

• 대한환경공학회지
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• 제32권12호
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• pp.1094-1101
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• 2010

낙동강, 회동 및 기장에서 채집한 sediment의 미생물 군집을 FISH 분석을 통하여 조사한 결과, ${\alpha}$ 그룹, Acidobacter 그룹 및 Cyanobacter 그룹의 분포비율이 가장 높았으며 전체적으로 서로 유사한 분포 특성을 나타내었다. 각각의 sediment를 접종한 MFC 농화배양 이후의 coulombic yield는 낙동강, 회동 및 기장의 경우 각각 0.64 C, 0.50 C, 0.61 C로 나타났으며, 농화배양 완료 후의 미생물 군집분포는 ${\beta}$-Proteobacteria, ${\gamma}$-Proteobacteria, Acidobacter 그룹 및 Firmicutes 그룹이 농화배양 전보다 각각 45~90%, 50~90%, 40~80% 및 45~125% 정도 생체량이 증가한 것으로 나타났다. 농화배양이 끝난 후 16S rDNA를 이용한 미생물 동정결과에서, 낙동강 sediment를 주입한 MFC의 경우는 ${\alpha}$-Proteobacteria의 속하는 Roseomonas sp., Azospillum sp.와 ${\gamma}$-Proteobacteria의 Frateuria sp., Dyella sp., Enterobacter sp.와 Deinococci 그룹의 Deinococcus sp.가 동정되었고, 기장 sediment는 ${\alpha}$-Proteobacteria의 Azospillum sp.와 ${\beta}$-Proteobacteria의 Delftia sp., Ralstonia sp.와 ${\gamma}$-Proteobacteria의 Klebsiella sp. 와 Deinococci 그룹의 Deinococcus sp.가 동정되었으며, 회동 sediment는 ${\gamma}$-Proteobacteria의 Pseudomonas sp., Klebsiella sp.와 Deinococci 그룹의 Deinococci sp.와 Actinobacteria 그룹의 Leifsonia sp.와 Bacilli 그룹의 Bacillus sp.가 동정되었다.

• The Korean Journal of Ecology
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• 제23권4호
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• pp.331-337
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• 2000

Fourteen different taxa of dictyostelid cellular slime molds were recovered from the alpine and subalpine zone in Mt. Paektu. In subalpine zone, six species were recovered : Dictyostelium minutum, D. brefeldianum, D. crassicaule, D. capitatum, Polysphondylium solitarium and P. pallidum. One of these species which were isolated from the soils of Larix olgensis community exhibited several distinctive features which differed from the published species. This species was designated as a new species, Polysphondylium solitarium Kang et Chang, sp. nov. When cultivated at 22~24$^{\circ}C$ on low-nutrient agar media with Escherichia coli, Polysphondylium solitarium is distinguishable from other species by the following combination of features: (ⅰ) the sorocarps with vinaceous pigmentation; (ⅱ) pseudoplasmodia radial. usually centralized and rarely subdivided; (ⅲ) the various number of whorls; (ⅳ) the spores with unconsolidated and nonconspicuous polar granules. Also, we confirmed this new species by analyzing ribosomal DNA (ITS1, ITS2 and 5.8s DNA) sequences of P. solitarium and P. violaceum.

• Mycobiology
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• 제42권4호
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• pp.401-404
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• 2014

A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.