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Distribution of Electrochemically Active Bacteria in the Sediment  

Son, Hyeng-Sik (Department of Microbiology, Pusan National University)
Son, Hee-Jong (Water Authority, Busan)
Kim, Mi-A (Department of Microbiology, Pusan National University)
Lee, Sang-Joon (Department of Microbiology, Pusan National University)
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Abstract
Microbial fuel cells (MFC) were enriched using sediment Nakdong river, Hoidong river and protected water area in Gijang. The microbial community of sediment and enriched MFC was analyzed by FISH (fluorescent in situ hybridization) and 16S rDNA sequencing. ${\alpha}$-Proteobacteria, Acidobacter and Cyanobactia group were dominant in sediment by FISH. The coulombs of the final 10 peak of the 3 MFC (Nakdong, Hoidong, Gijang) were 0.64 C, 0.50 C, 0.61 C, respectively. When MFCs were enriched by sediment, ${\beta}$-, ${\gamma}$-Proteobacteria, Acidobacter and Firmicutes group increased 45~90%, 50~90%, 40~80% and 45~125%, respectively. In results of 16S rDNA sequencing, Roseomonas sp., Azospillium sp., Frateuria sp., Dyella sp., Enterobacter sp. and Deinocossus were isolated from Nakdong river and Azospillium sp., Delftia sp., Ralstonia sp., Klebsiella sp. and Deinococcus sp. were isolated from protected water area in Gijang and Pseudomonas sp., Klebsiella sp., Deinococcus sp., Leifsonia sp. and Bacillus sp. were isolated from Hoidong river.
Keywords
Microbial Fuel Cell; Sediment; Microbial Community(MFC); FISH; 16S rDNA;
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