• 제목/요약/키워드: Irradiation intensity

검색결과 444건 처리시간 0.032초

NTacSam:SD 골수 세포의 증식에 100Hz PWM 저출력 광 조사가 미치는 효과 (Effect of the 100Hz PWM Low Power Light Irradiation in Proliferation of NTacSam:SD Bone-marrow Cell)

  • 천민우;김성환;이호식;박용필
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 춘계학술대회 논문집
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    • pp.10-11
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    • 2008
  • We developed the equipment palpating cell proliferation using a high brightness LED. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity, frequency and so on. Especially, to control the light irradiation frequency, FPGA was used, and to control the change of output value, TLC5941 was used. Control stage is divided into 30 levels by program. Consequently, the current value could be controlled by the change of level in Continue Wave(CW) and Pulse Width Modulation(PWM), and the output of a high brightness LED could be controlled stage by stage. And then, each experiment was performed to irradiation group and non-irradiation group for bone marrow cells. MIT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590nm transmittance of ELISA reader. As a result, the cell increase of bone marrow cells was verified in irradiation group as compared to non-irradiation group.

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이중 초음파 조사 시스템에서 진동부 사이의 거리가 초음파 화학 반응에 미치는 영향 (The Effect of Distance between Two Transducers on Sonochemical Reactions in Dual Irradiation Systems)

  • 김은경;손영규
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제18권5호
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    • pp.39-45
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    • 2013
  • Many researchers have studied the effectiveness of ultrasound in chemical and environmental engineering fields including material synthesis, pollutant removal, cleaning, extraction, and disinfection. Acoustic cavitation induced by ultrasound irradiation in aqueous phase can cause various sonophysical and sonochemical reactions without any chemicals. However most of the previous studies focused only on the relationships between ultrasonic conditions and the results of sonochemical reactions in lab-scale sonoreactors. As a results of this, only a few studies have been devoted to design and optimization of industrial scale sonoreactors. In this study, the effect of the distance between two opposite transducer modules on sonochemical reactions was investigated in single and dual irradiation systems (334 kHz) for four distances including 50, 100, 150, and 200 mm using KI dosimetry. It was found that the dual irradiation systems provided higher performance in terms of the zeroth reaction coefficient and the cavitation yield compared to the single irradiation systems. The sonochemiluminescence (SCL) images for the visualization of the cavitation field showed that cavitation active zone was larger and sonochemical reaction intensity was much higher in the dual irradiation system than in the single irradiation system.

광도가 서울 대기의 오존 생성 및 에어로졸 수 농도에 미치는 영향 (Effect of light intensity on the ozone formation and the aerosol number concentration of ambient air in Seoul)

  • 배귀남;박주연;김민철;이승복;문길주;김용표
    • 한국입자에어로졸학회지
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    • 제4권1호
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    • pp.9-20
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    • 2008
  • The effect of light intensity on the ozone formation and the aerosol number concentration during the photochemical reactions of ambient air was investigated in an indoor smog chamber. The smog chamber consists of a housing, 64 blacklights, and a $2.5-m^3$ reaction bag made of Teflon film. The bag was filled with the unfiltered ambient air in Seoul from January 10 to March 18, 2002. In this work, the photolysis rate of $NO_2$, $k_1$ was used as an index of light intensity. Three levels of light intensity were controlled by changing the number of blacklights turned on among 64 blacklights: $0.29min^{-1}$ (50%), $0.44min^{-1}$ (75%), $0.57min^{-1}$ (100%). The ozone concentration increased rapidly within 10 minutes after irradiation irrespective of light intensity, thereafter it increased linearly during the irradiation. The ozone production rate seems to be dependent on both the light intensity and the quality of ambient air introduced into the reaction bag. The change in aerosol number concentration also depended on both the light intensity and the ambient air quality, especially aerosol size distribution. Based on the initial ambient aerosol size distributions, the photochemical potential for aerosol formation and growth is classified into two cases. One is the case showing aerosol formation and growth processes, and the other is the case showing no apparent change in particle size distribution.

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자색고구마 캘러스배양에서 안토시아닌 생합성에 미치는 광의 영향 (Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato)

  • 박혜정;김정숙;박현용
    • Journal of Plant Biotechnology
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    • 제32권4호
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    • pp.307-311
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    • 2005
  • 자색고구마의 캘러스 배양에서 광의 조사가 안토시아닌 생합성에 미치는 효과를 실험하였다. 잎조직으로부터 자색캘러스는 $0.5\;{\mu}M$ 2,4-D가 첨가된 MS배지, 광조건에서 유도되었다. 광의 세기에 따른 효과는 암 조건에 비교하여 광조건에서 안토시아닌 생합성이 $2{\sim}3$배에 이르는 것으로 나타났으며, 약광 (3000 lux)에서 안토시아닌 생합성량이 높게 나타났다. 이러한 광 조사에 의한 안토시아닌 생합성의 촉진효과는 LED를 이용하며 실험한 결과에서 청색광을 2시간 처리한 경우 3000 lux의 백색광을 처리한 대구조에 비교하여 1.4배의 안토시아닌 생합성 향상효과를 나타냈다.

Effect of γ-Irradiation on the Molecular Properties of Bovine Serum Albumin and β-Lcatoglobulin

  • Cho, Yong-Sik;Song, Kyung-Bin
    • BMB Reports
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    • 제33권2호
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    • pp.133-137
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    • 2000
  • To elucidate the effect of oxygen radicals on the molecular properties of proteins, the secondary and tertiary structure and molecular weight size of BSA and ${\beta}$-lactoglobulin were examined after irradiation of proteins at various doses. Gamma-irradiation of protein solutions caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking, and aggregation of the polypeptide chains. As a model system, BSA and ${\beta}$-lactoglobulin were used as a typical ${\alpha}$-helical and a ${\beta}$-sheet structure protein, respectively. A circular dichroism study showed that the increase of radiation decreased the ordered structure of proteins with a concurrent increase of aperiodic structure content. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. SDS-PAGE and a gel permeation chromatography study indicated that radiation caused initial fragmentation of proteins resulting in a subsequent aggregation due to cross-linking of protein molecules.

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Stabilization of pitch-based carbon fibers accompanying electron beam irradiation and their mechanical properties

  • Park, Mi-Seon;Ko, Yoonyoung;Jung, Min-Jung;Lee, Young-Seak
    • Carbon letters
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    • 제16권2호
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    • pp.121-126
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    • 2015
  • Carbon fibers are prepared by stabilizing pitch fibers accompanying electron beam (E-beam) irradiation. The carbon fibers pretreated by E-beam irradiation achieve a higher stabilization index than the carbon fibers that are only heat-stabilized. In addition, the carbon fibers subjected to E-beam irradiation in the stabilization step exhibit a comparable tensile strength to that of general purpose carbon fibers. The carbon fibers pretreated with an absorbed dose of 3000 kGy have a tensile strength of 0.54 GPa for a similar fiber diameter. Elemental, Fourier-transform infrared spectroscopy, and thermogravimetric analyses indicate that E-beam irradiation is an efficient oxidation and dehydrogenation treatment for pitch fibers by showing that the intensity of the aliphatic C-H stretching and aromatic $CH_2$ bending (out-of-plane) bands significantly decrease and carbonyl and carboxylic groups form.

저강도 레이저 조사에 의한 가토 피부의 상처 치유에 관한 연구 (The Study on Wound Healing in Rabbit Skins by Low-intensity Laser Irradiation)

  • 김식현;전진석
    • 대한의생명과학회지
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    • 제6권2호
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    • pp.119-129
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    • 2000
  • 피부는 인체의 표면을 보호하는 중요한 기관으로 피부가 손상되었을 경우 상처 재생은 염증기, 상피화기, 수복기의 정상적인 재생 단계를 거치며 치유된다 최근 저강도 레이저의 생물학적 효과로서 상처 재생과의 밀접한 관련성이 알려지고 있다. 본 연구는 저강도 레이저가 상처 재생에 미치는 유의한 효과를 세포 형태학적으로 확인하기 위해 실험적으로 유도한 가토 피부 상처 (2$\times$2 cm)에 12일 동안 5 Hz, 830 nm, 1.6 J/$cm^2$의 자극강도 (10 min/day)로 상처면에 레이저를 적용한 결과, 다음과 같은 곁과를 얻었다. 레이저 조사군의 경우 결합조직의 수복과 상피의 재형성이 대조군과 비교했을 매우 빠르게 진행되는 것으로 관찰되었으며, 특히 섬유아세포의 활성과 육아조직 합성율이 유의하게 증가되는 것으로 확인되었다. 이상의 연구 곁과를 종합해 달 때 유효한 치료강도의 저강도 레이저 자극은 피부의 개방성 창상 및 욕창 등의 상처 치유를 촉진할 수 있는 것으로 사료된다.

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건마늘과 건양배추의 방사선 조사여부 확인을 위한 물리적 마커 분석 (Analyzing a Physical Marker to Identify Irradiated Dried Garlic and Cabbage)

  • 김동길;안재준;;이호천;권중호
    • 한국식품과학회지
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    • 제41권2호
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    • pp.136-140
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    • 2009
  • 건마늘과 건양배추에 대하여 감마선 조사(0, 2.5, 5, 10, 20 kGy)에 따른 검지특성을 PSL, ESR 및 TL 분석에 의해 확인하였다. PSL 분석결과, 건마늘과 건양배추의 비 조사시료는 287-337 photon counts로 negative값을 나타내었고, 조사시료는 7511-54063 photon counts의 positive값을 나타내어 비 조사시료와 조사시료간의 차이를 확인할 수 있었다. ESR 분석 결과, 건마늘의 경우 비 조사시료에서 나타나지 않은 cellulose radical이 고선량(20 kGy)에서 관찰되었고, 건양배추의 경우 조사시료에서 crystalline sugar 유래의 multi- component signal이 관찰되었다. 또한 조사선량이 증가할수록 ESR signal intensity도 유의적으로 증가하였다($R^2$= 0.9369-0.9926). TL 분석 결과, 건마늘과 건양배추 모두 비 조사시료와 조사시료의 glow curve가 나타나는 온도 범위(150-250$^{\circ}C$)와 TL intensity의 차이가 명확하였고, 조사선량의 증가에 따라 TL signal intensity가 증가하였다($R^2$= 0.9670-0.9768). 그리고 TL ratio($TL_1/TL_2$)의 측정결과는 비 조사구는 0.1 이하, 조사구는 0.1 이상의 값을 나타내어 TL 분석결과의 신뢰성을 높여주었다.

470nm LED 광이 RAT 피부결손에 미치는 효과 (The effect of 470nm LED Irradiation on skin injury of RAT)

  • 천민우;김성환;박용필;이호식;김영표;김태곤;이희갑
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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    • pp.416-417
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    • 2008
  • This paper performed the basic study for developing the Photodynamic Therapy Equipment for medical treatment. We developed the 4channel light medical therapy apparatus for external injury cure using a LED light source. This equipment was fabricated by using microcontroller, and designed to enable us to control light irradiation timer, intensity and reservation. In this paper, the designed device was used to find out how 470nm LEDs light source affects the skin wound of RAT. In the experiment, $1cm_2$ wounds on the External injury of RAT were made. Light irradiation RAT and none light irradiation RAT divided, each RAT was irradiated one hour a day for 15 days. In result, compared with none light irradiation RAT, the lower incidence of inflammation and faster recovery was shown in light irradiation RAT.

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전자빔 공정을 적용한 Triclosan의 제거특성 및 독성평가 (Toxicity Assessment and Decomposition Characteristics of Triclosan in an E-beam Irradiation Process)

  • 장태범;장순웅;이시진;조일형
    • 한국지반환경공학회 논문집
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    • 제13권3호
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    • pp.5-11
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    • 2012
  • 본 연구의 목표는 전자빔 공정을 적용하여 Triclosan의 제거효율 및 독성평가를 하는 것이다. 이 실험에서는 전자빔 선량과 Triclosan의 초기 농도에 따른 저감 효율 및 스캐벤저 가스에 따른 무기화의 정도를 알아보았다. 또한 녹조류 중 하나인 Pseudokirohneriella Subcapitata를 이용하여 생물독성과 위해성 평가를 통한 독성저감기술을 시험하였다. 그 결과 Triclosan은 전자빔 조사량이 증가할수록 분해 효율이 향상되었고, 라디칼 스캐벤저 가스에 의한 TOC 제거는 $N_2O$$O_2$를 용해시켰을 때 효율이 증가된 것으로 나타났다. 4일(96hrs) 후 독성평가 결과 전자빔 조사 강도의 증가에 따라 독성 영향이 저감되는 것을 알 수 있었다.