• 대한의용생체공학회:의공학회지
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• 제19권1호
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• pp.81-90
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• 1998

전자파는 열적 또는 비열적 작용에 의해 신경조직에 다양한 영향을 미친다. 본 논문에서는 미세한 전자파에 노출되기 전에 칼슘통로차단제인 nimodipine 처리를 한 토끼의 EEG를 측정함으로써 전자파 조사시 중추신경계의 반응 및 그것에 대한 nimodipine의 영향을 평가하려고 하였다. 10마리씩 이루어진 두 집단의 토끼에 각기 10dBm, 20dBm의 전력밀도를 가진 2,450 MHz의 전자파를 10분간 조사한 후 EEG 신호를 측정하여 전자파에 노출되지 않은 다른 10 마리의 토끼의 EEG 신호와 비교하였다. 그다음 20 dBm의 전자파에 노출시키기 전에 nimodipine을 정맥주사한 토끼의 뇌파를 생리식염수만 주사한 토끼의 뇌파와 비교하였다. 10dBm의 전자파 조사시에는 뇌파의 변화가 없었으나, 20 dBm의 전자파 조사시 EEG 전압의 감소 및 delta bank의 현저한 감소와 alpha 및 beta band 의 현저한 감소와 alpha 및 beta band의 증가를 알아낼 수 있었다. 그러나 국소적인 혈관 주위 염증반응 이외의 뇌세포의 이상소견은 관찰되지 않았다. 그리고 이러한 변화는 nimodipine 처리에 따른 차이를 보이지 않았다. 전자파조사시 nimodipine처리한 토끼의 뇌파에서 처리하지 않은 토끼와의 유의한 차이가 관찰되지 못했기 때문에 이러한 효과는 열적 효과로 보여진다.

• Journal of Oral Medicine and Pain
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• 제19권1호
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• pp.73-91
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• 1994

The author used rabbits in order to examine the effect of Ga-As low power generating semiconductor laser on artificially produced injuries of experimental animals. Artificially produced injuries include surgical wound of 3mm length, 2mm depth in size on ventral skin surface of rabbit and buccal mucosa, and electrical injury formed on opposite side of skin and buccal mucosa by electrical cauterization of same length and depth, and chemical injury formed by FC(Formocresol) solution applied on the anterior dorsal part of tongue. And then, on the experimental group, Ga-As laser was irradiated beginning on the day after the wound formation and continued to irradiate every each other day for five minutes. After1, 3, 6, 9, 13th day, certain number of animals of control and experimental group were sacrified, and wound site tissue was excised to make samples and was observed under light microscope. The following is the conclusions after comparing the healing procedure of experimental and control group. The following results were obtained : 1. Inflammation was decreased more rapidly in the experimental group than the control group. 2. In the surgical, the electrical and the chemical injuries in the oral mucosa, re-epithelialization was completed more rapidly in the experimental group than the control group. In the electrical injury on the skin, re-epithelialization was completed about 6 days after wound formation on both groups. 3. In the electrical and the surgical injuries on the oral mucosa, granulation tissue formation started at 3 days after injury on both groups, but in the chemical injury, it was completed about 3 days faster in the control group than the experimental group. In the surgical wound on the skin, it was completed about 9 days after injury, but faster in the experimental group. In the electrical injury on the skin, it was faster in the control group than the experimental group. 4. In the electrical and the surgical injuries on the oral mucosa, fibrosis was started at 6~9 days after injury on both groups, but regeneration of connective tissue in the experimental group was observed much more than the control group. 5. When comparing the effect of wound healing on skin and oral mucosa of control and experimental group, granulation tissue formation and re-epithelialization in the oral mucosa was more vigorous. In conclusion, the difference of timing and the sequence of wound healing process(inflammation, re-epithelialization, granulation tissue formation, fibrosis) following Laser irradiation between control and experimental group was not observed, but the healing tissue was observed much more in the Laser irradiated group.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.262-269
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• 2005

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.524-528
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• 2001

The measurement of radiation response using simple and informative techniques would be of great value in studying the genetic risk following occupational, therapeutic, or accidental exposure to radiation. When patients receive radiation therapy, many suffer from side effects. Since each patient receives a different dose due to different physical conditions, it is important to measure the exact dose of radiation received by each patient to lessen the side effects. Even though several biological dosimetric systems have already been developed, there is no ideal system that can satisfy all the criteria for an idean dosimetric system, especially for low-dose radiation as used in radiation therapy. In this study, an SOS Chromotest of E. coli PQ37 was evaluated as a novel dosimeter for low-dose gamma-rays. E. coli PQ37 was originally developed to screen chemical mutagens using the SOS Chromotest-a colorimtric assay, based on the induction of ${\beta}$-galactosidase ue to DNA damage. The survival fraction of E. coli PQ37 decreased dose-dependently with an increasing dose of cobalt-60 gamma-rays. Also, a good linear correlation was found between the biological damage revealed by the ${\beta}$-galactosidase expression and the doses of gamma-rays. The expression of ${\beta}$-galactosidase activity that responded to low-dose radiation under 1 Gy was $Y=0.404+(0.089{\pm}0.3)D+(-0.018{\pm}0.16)D^2$ (Y, absorbance at 420 nm; D, Dose of irradiation) as calculated using Graph Pad In Plot and Excel. When a rabbit was fed with capsules containing an agar block embdded with E. coli PQ37 showed a linear response to the radiation doses. Accordingly, the results confirm that E. coli PQ37 can be used as a sensitive biological dosimeter fro cobalt-60 gamma-rays. To the best of our knowledge, this is the first time that a bacterium has been used as a biological dosimeter, especially for low-dose radiation.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 춘계학술발표대회
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• pp.53.1-53.1
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• 2011

Bioactive glass powders were synthesized by hydrothermal chemical route by the use of ultrasonic energy irradiation. We used sodalime, calcium nitrate tetra hydrate and di ammonium hydrogen phosphate as the precursor material to synthesize $SiO_2$ rich bio-active glass materials. The $SiO_2$ content was varied in the precursor mixture to 60, 52 and 45 mole%. Dense compacts were obtained by microwave sintering at $1,100^{\circ}C$. Mechanical properties were characterized for the fabricated dense bioactive glasses and were found to be comparable with conventional CaO-$SiO_2$-$Na_2O$-$P_2O_5$ bioactive glass. Detailed biocompatibility evaluation of the glass composition was investigated by in-vitro culture of MG-63 cell and mesenchyme stem cell. Cell adhesion behavior was investigated for both of the cell by one cell morphology for 30, 60 and 90 minutes. Cell proliferation behavior was investigated by culturing both of the cells for 1, 3 and 7 days and was found to be excellent. Both SEM and confocal laser scanning microscopy were used for the investigation. Western blot analysis was performed to evaluate the bimolecular level interaction and extent and rate of specific protein expression. The ability to form biological apatite in physiological condition was observed with simulated body fluid (SBF). In-vivo bone formation behavior was investigated after implanting the materials inside rabbit femur for 1 and 3 month. The bone formation behavior was excellent in all the bioglass compositions, specially the composition with 60% $SiO_2$ content showed most promising trend.

• 한국식품과학회지
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• 제32권3호
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• pp.720-725
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• 2000

여러 가지 처리가 난백의 항원성을 감소시키는데 미치는 영향을 조사하기 위하여, 토끼 항난백항체로 난백 처리물들의 간접 경합 ELISA(ciELISA)를 실시하였다. 식물성, 동물성, 미생물성의 9가지 효소를 이용한 가수분해처리는 난백의 항원성에 영향을 주지 못했다. 보다 효율적인 효소 가수분해를 위해 방사선 조사 후, 효소처리를 실시했는데, 이것 역시 항원성을 감소시키지 못했다. Trifluoromethanesulfonic acid 처리는 난백의 항원성을 거의 제거시키는 것으로 나타났다. 열처리는 $121^{\circ}C$ 고온처리의 경우에서만 반응성이 1/10,000이하로 감소하여 항원성이 거의 사라졌다. NaOH 단독 처리는 0.3%(w/v) 이상의 농도에서 반응성이 감소되기 시작하여, 1%(w/v) 이상에서는 반응성이 1/10,000이하로 감소하였다. NaOH 처리에 $70^{\circ}C$, 15분간의 열처리를 더해 주면, NaOH 단독 처리시보다 효과적으로 항원성이 감소되는 것으로 나타났다.

• Current Optics and Photonics
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• 제5권3호
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• pp.311-321
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• 2021

Indocyanine green (ICG) is a dye approved for use in clinical diagnostics. ICG remains in the intravascular space following intravenous administration, due to its ability to rapidly bind to the plasma proteins, and its therapeutic potential has been studied in well-vascularized cutaneous tumors. Here we have evaluated the clinical response of a subconjunctival tumor to photothermal therapy (PTT) using an ICG-enhanced near-infrared diode laser and its adverse effects, in a rabbit. 22 male New Zealand white rabbits with subconjunctival tumors were enrolled (control group 6, laser-only group 8, laser-with-ICG group 8). Rabbits in the laser-with-ICG group received ICG (twice, 2 mg/kg each time, intravenously) directly followed by irradiation with a diode laser (λ = 810 nm). Rabbits in the laser-only group were irradiated with the diode laser. ICG angiography, ultrasonography, and pathologic examination were performed to evaluate PTT response at specific time points (0, 2, and 4 weeks after PTT). Two weeks after initial treatment, the eight rabbits treated by laser with ICG showed a 100% response rate. There was no clinical response in both laser-only and control groups. ICG-PTT is a potential and effective palliative therapeutic modality for subconjunctival tumors.

• 한국임상수의학회지
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• 제32권4호
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• pp.295-300
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• 2015

본 연구의 목적은 알지네이트 비드에서 배양한 토끼의 관절 연골세포의 증식과 황산화 글리코스아미노글리칸 합성에 대한 808-nm InGaAs 다이오드 레이저의 효과를 확인하는 것이다. 이전의 연구들에서 서로 다른 종류의 세포에서 레이저의 양성 또는 음성 자극 효과가 알려졌다. 알지네이트 비드 내의 토끼 연골세포에 1.0W 세기의 808-nm InGaAs 다이오드 레이저가 $31J/cm^2$ (1 그룹), $62J/cm^2$ (2 그룹)의 에너지 밀도로 상응하는 그룹에 10초, 20초 동안 24, 48, 72, 96시간째에 각각 조사되었다. 대조군은 처리하지 않았다. 1차 레이저 조사 1주, 2주 후에 MTT 분석이 실시되었다. 황산화 글리코스아미노글리칸 합성은 DMMB 분석을 통해 평가되었다. 조직학적 평가를 위한 세포의 분포와 세포 주변의 황산화 글리코스아미노글리칸 침착은 알시안 블루 염색을 통해 평가되었다. MTT 분석을 통해 알지네이트 비드에서 세포 증식에는 양성 자극 효과가 없음을 알 수 있었다. DMMB 분석을 통해서 2 그룹의 2주차 연골세포에서 황산화 글리코스아미노글리칸 생성이 특이적으로 증가했음을 알 수 있었다. 알시안 블루 염색상에서도 2 그룹 연골세포에서 양성 염색상이 특이적으로 많은 비율을 차지함을 알 수 있었다. 본 연구를 통해 1.0 W 세기의 808-nm InGaAs 다이오드 레이저가 연골세포 증식에 영향이 없으나 알지네이트 비드에서 세포 분비 활동을 자극하여 황산화 글리코스아미노글리칸 침착을 증가시킬 수 있음을 확인하였다.

• 한국식품과학회지
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• 제40권3호
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• pp.343-348
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• 2008

여러가지 방법으로 난백을 처리하고 난백 중 주요한 알레르겐인 OM의 알레르기성 변화를 계란 알레르기 환자의 IgE 항체를 이용한 간접경합 ELISA로 조사하였다. 12종의 효소를 이용한 가수분해, 방사선조사, succinic anhydride 처리는 OM의 알레르기성을 효과적으로 감소시키지 못 하였다. OM의 당쇄를 제거하는 TFMS의 처리는 OM의 알레르기성을 1/20 정도로 감소시켰다. $100^{\circ}C$에서 10분간의 열처리는 알레르기성을 감소시키지 못하였으나 $121^{\circ}C$(10분)의 처리는 난백 중 OM의 알레르기성을 1/100정도로 감소시켰다. NaOH 단독 처리는 3%(w/v) 이상에서 알레르기성이 대부분 사라졌고, NaOH(0.3%(w/v) 이상)와 열($70^{\circ}C$,15 min)의 복합처리는 알레르기성을 1/10,000 이하로 낮춤으로써 가장 효과적인 감소를 보였다. 본 연구에서 환자의 IgE 항체를 이용한 ELISA 분석결과와 동일 시료에 대한 토끼 IgG 항체의 ELISA 결과는 대체적으로 비슷한 결과를 보였지만, TFMS 처리물에 대한 반응성은 후자에서 훨씬 많이 감소되어(1/10,000) 대조적이었다. 이는 식품의 항원성과 알레르기성 간에는 약간의 차이가 있음을 시사하였다.

• 대한핵의학회지
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• 제5권2호
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• pp.19-40
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• 1971

Since the iron balance is maintained by regulated intestinal absorption rather than regulated excretion, there have been many reports concerning the factors which may influence the intestinal iron absorption. As the liver is the largest iron storage organ of the body, any hepatocellular damage may result in disturbances in iron metabolism, e,g., frequent co-existence of hemochromatosis and liver cirrhosis, or elevated serum iron level and increased iron absorption rate in patients with infectious hepatitis or cirrhosis. In one effort to demonstrate the influence of hepatocellular damage on intestinal iron absortion, the iron absorption rate was measured in the rabbits whose livers were injured by a single subcutaneous injection of carbon tetrachloride (doses ranging from 0.15 to 0.5cc per kg of body weight) or by a single irradiation of 2,000 to 16,000 rads with $^{60}Co$ on the liver locally. A single oral dose of $1{\mu}Ci\;of\;^{59}Fe$-citrate with 0.5mg of ferrous citrate was fed in the fasting state, 24 hours after hepatic damage had been induced, without any reducing or chelating agents, and stool was collected for one week thereafter. Serum iron levels, together with conventional liver function tests, were measured at 24, 48, 72, 120 and 168 hours after liver damage had been induced. All animals were sacrificed upon the completing of the one week's test period and tissue specimens were prepared for H-E and Gomori's iron stain. Following are the results. 1. Normal iron absorption rate of the rabbit was $41.72{\pm}3.61%$ when 0.5mg of iron was given in the fasting state, as measured by subtracting the amount recovered in stool collected for 7 days from the amount given. The test period of 7 days is adequate, for only 1% of the iron given was excreted thereafter. 2. The intestinal iron absorption rate and serum iron level were significantly increased when the animal was poisoned by a single subcutaneous injection of 0.15cc. per kg. of body weight of carbon tetrachloride or more, or the liver was irradiated with a single dose of 12,000 rads or more. The results of liver function tests which were done simultaneously remained within normal limit except SGOT and SGPT which were somewhat increased. 3. In each case, there has been good correlation between the extent of liver cell damage and degree of increased iron absorption rate or serum iron level. 4. The method of liver damage appeared to make no obvious difference in the pattern of iron deposit in liver. This may be partly due to the fact that tissue specimens were obtained too late, for by this time the elevated serum iron level had returned within normal range and the pathological changes were almost healed. 5. The possible factors and relationship between intestinal iron absorption and hepatic parenchymal cell damage has been discussed.