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http://dx.doi.org/10.17555/jvc.2015.08.32.4.295

In Vitro Effect of 808-nm Diode Laser on Proliferation and Glycosaminoglycan Synthesis of Rabbit Articular Chondrocytes  

Minar, Maruf (College of Veterinary Medicine, Chungbuk National University)
Hwang, Ya-won (College of Veterinary Medicine, Chungbuk National University)
Choi, Seok-hwa (College of Veterinary Medicine, Chungbuk National University)
Kim, Gonhyung (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.32, no.4, 2015 , pp. 295-300 More about this Journal
Abstract
The aim of the study was to assess the in vitro effect of 808-nm InGaAs diode laser on rabbit articular chondrocyte proliferation and sulphated glycosaminoglycan (sGAG) synthesis in alginate bead. Previous studies revealed either positive or negative stimulatory effects of laser on different types of cells. A 808-nm InGaAs diode laser at 1.0W power output was used to irradiate the rabbit chondrocytes in alginate beads with energy densities of $31J/cm^2$ (G 1) and $62J/cm^2$ (G 2) corresponding to the experimental groups for 10 seconds and 20 seconds, respectively at 24, 48, 72 and 96 hours after seeding. Control group was left untreated. MTT assay was performed at 1 week and 2 weeks after the $1^{st}$ laser irradiation in alginate beads. sGAG synthesis in alginate beads at 1 week and 2 weeks were determined by DMMB assay. Histological evaluation for cellular distribution and sGAG deposition around the cells were performed by alcian blue stain. MTT assay revealed no positive stimulatory effect in cell proliferation in alginate bead. DMMB assay results showed significantly increased sGAG production in G 2 chondrocytes at 2 weeks. Image analysis of alcian blue stained slides also showed significantly higher percentage of positive alcian blue stain in G 2 chondrocytes. This result suggests that 808-nm InGaAs diode laser with 1.0 W power output although cannot stimulate cell proliferation it can increase the cell secretion activity and sGAG deposition in alginate beads.
Keywords
Diode/diode laser; Orthopaedic; Tissue regeneration and healing;
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1 AlGhamdi KM, Kumar A, Moussa NA. Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 2012; 27: 237-249.   DOI
2 Arzi B, Wisner ER, Huey DJ, Kass PH, Hu J, Athanasiou KA. A proposed model of naturally occurring osteoarthritis in the domestic rabbit. Lab Anim (NY) 2012; 41: 20-25.   DOI
3 Brosseau L, Welch V, Wells G, Tugwell P, de Bie R, Gam A, Harman K, Shea B, Morin M. Low level laser therapy for osteoarthritis and rheumatoid arthritis: a metaanalysis. J Rheumatol 2000; 27: 1961-1969.
4 Conlan MJ, Rapley JW, Cobb CM. Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 1996; 23: 492-496.   DOI
5 Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC, Yum LW. The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 2001; 4: 3-14.   DOI
6 Eduardo Fde P, Bueno DF, de Freitas PM, Marques MM, Passos-Bueno MR, Eduardo Cde P, Zatz M. Stem cell proliferation under low intensity laser irradiation: a preliminary study. Lasers Surg Med 2008; 40: 433-438.   DOI
7 Gasparyan VC. Method of determination of aortic valve parameters for its reconstruction with autopericardium: An experimental study. J Thorac Cardiovasc Surg 2000; 119: 386-387.   DOI
8 Haas AF, Isseroff RR, Wheeland RG, Rood PA, Graves PJ. Low-energy helium-neon laser irradiation increases the motility of cultured human keratinocytes. J Invest Dermatol 1990; 94: 822-826.   DOI
9 Huang YY, Chen AC, Carroll JD, Hamblin MR. Biphasic dose response in low level light therapy. Dose Response 2009; 7: 358-383.   DOI
10 Impellizeri JA, Tetrick MA, Muir P. Effect of weight reduction on clinical signs of lameness in dogs with hip osteoarthritis. J Am Vet Med Assoc 2000; 216: 1089-1091.   DOI
11 Karu TI, Pyatibrat LV, Kalendo GS, Esenaliev RO. Effects of monochromatic low-intensity light and laser irradiation on adhesion of HeLa cells in vitro. Lasers Surg Med 1996; 18: 171-177.   DOI
12 Liu H, Lee YW, Dean MF. Re-expression of differentiated proteoglycan phenotype by dedifferentiated human chondrocytes during culture in alginate beads. Biochim Biophys Acta 1998; 1425: 505-515.   DOI
13 Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S. Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 1998; 22: 97-102.   DOI
14 Marijnissen WJ, van Osch GJ, Aigner J, van der Veen SW, Hollander AP, Verwoerd-Verhoef HL, Verhaar JA. Alginate as a chondrocyte-delivery substance in combination with a non-woven scaffold for cartilage tissue engineering. Biomaterials 2002; 23: 1511-1517.   DOI
15 Pinheiro AL, Carneiro NS, Vieira AL, Brugnera A Jr, Zanin FA, Barros RA, Silva PS. Effects of low-level laser therapy on malignant cells: in vitro study. J Clin Laser Med Surg 2002; 20: 23-26.   DOI
16 Mester E, Mester AF, Mester, A. The biomedical effects of laser application. Lasers Surg Med 1985; 5: 31-39.   DOI
17 Ohgushi H, Goldberg VM, Caplan AI. Repair of bone defects with marrow cells and porous ceramic. Experiments in rats. Acta Orthop Scand 1989; 60: 334-339.   DOI
18 Pelletier JP, Lajeunesse D, Hilal G, Jovanovic D, Fernandes JC, Martel-Pelletier J. Carprofen reduces the structural changes and the abnormal subchondral bone metabolism of experimental osteoarthritis. Osteoarthritis Cartilage 1999; 7: 327-328.   DOI
19 Pogrel MA, Chen JW, Zhang K. Effects of low-energy gallium-aluminum-arsenide laser irradiation on cultured fibroblasts and keratinocytes. Lasers Surg Med 1997; 20: 426-432.   DOI
20 Saito S, Shimizu N. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofacial Orthop 1997; 111: 525-532.   DOI
21 Smith K. Light and life: the photobiological basis of the therapeutic use of radiation from lasers, in: ILTA Congress: Progress in laser therapy. Ohshiro T, Calderhead RG. (eds.). Okinawa: Wiley 1990.
22 Soffa AJ, Markel MD, Converse LJ, Massa KL, Bogdanske JJ, Dillingham MF. Treatment of inflammatory arthritis by synovial ablation: a comparison of the holmium: YAG laser, electrocautery, and mechanical ablation in a rabbit model. Lasers Surg Med 1996; 19: 143-151.   DOI
23 Son J, Kim YB, Ge Z, Choi SH, Kim G. Bone healing effects of diode laser (808 nm) on a rat tibial fracture model. In Vivo 2012; 26: 703-709.
24 Yu HS, Chang KL, Yu CL, Chen JW, Chen GS. Lowenergy helium-neon laser irradiation stimulates interleukin-1 alpha and interleukin-8 release from cultured human keratinocytes. J Invest Dermatol 1996; 107: 593-596.   DOI
25 Stein A, Benayahu D, Maltz L, Oron U. Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 2005; 23: 161-166.   DOI
26 Torricelli P, Giavaresi G, Fini M, et al. Laser biostimulation of cartilage: in vitro evaluation. Biomed Pharmacother 2001; 55: 117-120.   DOI
27 Wu JY, Wang YH, Wang GJ, Ho ML, Wang CZ, Yeh ML, Chen CH. Low-power GaAlAs laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via IGF1 and BMP2. PLoS One 2012; 7: e44027.   DOI
28 Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD. Effect of wavelength on low-intensity laser irradiationstimulated cell proliferation in vitro. Lasers Surg Med 2005; 36: 8-12.   DOI
29 Bibikova A, Oron U. Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 1993; 235: 374-380.   DOI
30 Jia YL, Guo ZY. Effect of low-power He-Ne laser irradiation on rabbit articular chondrocytes in vitro. Lasers Surg Med 2004; 34: 323-328.   DOI
31 Shefer G, Partridge TA, Heslop L, Gross JG, Oron U, Halevy O. Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells. J Cell Sci 2002; 115: 1461-1469.