• Biomolecules & Therapeutics
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• 제10권4호
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• pp.293-302
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• 2002

The classical concept for iron uptake into mammalian cells has been the endocytosis of transferrin( $T_{f}$ )-bound F $e^{3+}$ via the $T_{f}$ - $T_{f}$ receptor cycle. In this case, we could not explain the uptake of F $e^{2＋}$ ion and the export of iron from endosome. Studies on iron transport revealed that other transport system exists in epithelial cells of the intestine. One of non- $T_{f}$ -receptor-mediated transport systems is Nramp2/DMT1/DCT1 which transports M $n^{＋＋}$, $Mg^{＋＋}$, Z $n^{＋＋}$, $Co^{＋＋}$, N $i^{＋＋}$ or C $u^{＋＋}$ ion as well as F $e^{+2}$ ion. DMT1 was cloned from intestines of iron-deficient rats and shown to be a hydrogen ion-coupled iron transporter and a protein regulated by absorbed dietary iron. DMT1 is founded in other cells such as cortical and hippocampal glial cells as well as endothelial cells in duodenum. Two F $e^{3+}$ ion bound to transferrin( $T_{f}$ ) are taken up via the $T_{f}$ - $T_{f}$ receptor cycle in the intestinal epithelial cell. F $e^{3+}$ in endosome was converted to F $e^{2＋}$ ion, and then exported to cytosol via DMT1. F $e^{2＋}$ ion is taken up into cytosol via DMT1. Several other transporters such as FET, FRE, CCC2, AFT1, SMF, FTR, ZER, ZIP, ZnT and CTR have been reported recently and dysfunction of the transporters are related with diseases containing Wilson's disease, Menkes disease and hemochromatosis. Evidences from several studies strongly suggest that DMT1 is the major transporter of iron in the intestine and functions critically in transport of other metal ions.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.39-39
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• 2001

Many physiological processes of plant cells, such as nutrient uptake, salt tolerance, and cell enlargement, are mediated by ion transports across the plasma membrane. H$^{＋}$-ATPases on both plasma and vacuolar membranes play major roles on active transports and ion channels mediate passive transports of various ions. It has been known that these proteins involved in cellular osmotic regulation and salt tolerance in the salt-accumulated soils.(omitted)

• The Korean Journal of Ecology
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• 제6권4호
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• pp.243-249
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• 1983

Absorption pattern of potassium, calcium, phosphate and nitrate ions, and the pH change during ion-absorption at pH 3.0-11.0 by Oryza sativa L. were studied to investigate indirectly the evidence of H^+-efflux by ATPase. The rice seedlings which were grown either in $L^{\circ} -dark or in L^+-sunlight$, were used both in each ion-absorption to compare with one another. The uptake rate of these ions appeared to favor more in $L^+than in L^{\circ}$, over all range in pH, nearly with the same pattern. The absorption of potassium resulted in bell shape and that of calcium increased linearly to the alkali range. The shape of phosphate-absorption showed nearly the t-distribution curve with high value in acid range and the uptake of nitrate resulted in the dual peaks, but higher in acid range. The pH of the external solution changed from the range of 3.5-11.0 to 3.5-7.0 after lhr-absorptioin, and further acidified after 3hr-absorption. It is suggested that the pH change of the external solution be affected by $ H^+-efflux$ which may be caused by the ATP-hydrolysis.

• 폴리머
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• 제26권5호
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• pp.575-581
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• 2002

본 연구는 폴리에틸렌 기재에 비드와 섬유상 이온교환체의 배합비를 달리하여 불균질 이온 교환막을 제조하였다. PE 불균질 이온교환막의 이온교환능은 섬유강 이온교환체의 양이 증가할수록 선형적으로 증가하였으며 30 wt%에서 최대 1.86 meq/g 이었다. 불균질 이온교환막의 함수율, 고정이온농도 및 이온운반율도 섬유상 이온교환체의 양이 증가할수록 증가하였나. 반면 불균질 이온교환막의 전기저항은 섬유상 이온교환체의 양이 증가할수록 감소하였으며, IXF (ion exchange fiber)의 혼합량이 30 wt%에서 5$\Omega$/$\textrm{cm}^2$으로 전기저항 값이 가장낮았다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.109-114
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• 2005

Ion fluxes across the plasma membrane activated by 1 mM $Ce^{4+}$, cell apoptosis and taxol biosynthesis in suspension cultures of Taxus cusp/data were studied. The extracellular pH sharply decreased upon the addition of 1 mM $Ce^{4+}$, then increased gradually and exceeded the initial pH value over a time period of 12 h. The extracellular $Ca^{2+}$ concentration decreased within the first 3 h after the addition of $Ce^{4+}$, then gradually decreased to one third of initial value in control at about 72 h and remained unchanged afterwards. Experiments with an ion channel blocker and a $Ca^{2+}$-channel blocker indicated that the dynamic changes in extracellular pH and the $Ca^{2+}$ concentration resulted from the $Ce^{4+}$-induced activation of W uptake and $Ca^{2+}$ influx across the plasma membrane via ion channels. A pretreatment of the ion channel blocker initiated $Ce^{4+}$-treated cells to undergo necrosis, and the prior addition of the $Ca^{2+}$-channel blocker inhibited $Ce^{4+}$-induced taxol biosynthesis and apoptosis. It is thus inferred that W uptake is necessary for cells to survive a $Ce^{4+}$-caused acidic environment and is one of the mechanisms of $Ce^{4+}$-induced apoptosis. Furthermore, the $Ca^{2+}$ influx across the plasma membrane mediated both the $Ce^{4+}$-induced apoptosis and taxol biosynthesis.

• 한국환경과학회지
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• 제20권11호
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• pp.1437-1445
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• 2011

The adsorption performance of lead ion was studied using five zeolites (Na-P1, sodalite (SOD), analcime (ANA), nepheline hydrate (JBW), cancrinite (CAN)) synthesized from Jeju scoria. The adsorption performances of lead ion decreased in the order of Na-P1 > SOD > ANA > JBW > CAN. These results showed that the synthetic zeolite with a higher cationic exchange capacity showed a higher adsorption performance. The uptake of lead ion by synthetic zeolites were described by Freundlich model better than Langmuir model. The adsorption kinetics of lead ion by synthetic zeolites fitted the pseudo 2nd order kinetics better than pseudo 1st order kinetics. The effective diffusion coefficients of lead ion by synthetic zeolites were ten times higher than the zeolite A synthesized from coal fly ash.

• Nuclear Engineering and Technology
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• 제55권10호
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• pp.3665-3676
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• 2023

The use of ion exchange resins to remove ionic impurities from solution is prevalent in industrial process systems, including in the primary heat transport system (PHTS) purification circuit of nuclear power plants. Despite its extensive use in the nuclear industry, our general understanding of ion exchange cannot fully explain the complex chemistry in ion exchange beds, particularly when operated at or near their saturation limit. This work investigates the behaviour of mixed-bed ion exchange resin, saturated with species representative of corrosion products in a CANDU (Canadian Deuterium Uranium) reactor PHTS, particularly with respect to iron chemistry in the resin bed and the removal of lithium ions from solution. Experiments were performed under deaerated conditions, analogous to normal PHTS operation. The results show interesting iron chemistry, suggesting the hydrolysis of cation resin bound ferrous species and the subsequent formation of either a solid hydrolysis product or the soluble, anionic Fe(OH)₃^-.

• 한국수소및신에너지학회논문집
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• 제28권2호
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• pp.144-149
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• 2017

A proton-conducting bisphenylpropaned sulfonated fluorinated blockcopolymer (BPSFBC) was synthesized. Five kinds of polymer electrolyted composite membranes were preparated by incorporating silica ($SiO_2$) with various weight ratio. And their characteristics were investigated by FT-IR (fourier transform infrared), $^1H-NMR$ ($^1H$ nuclear magnetic resonance), TGA (thermogravimetric analysis), water uptake, FE-SEM (field emission scanning electron microscopes), and ion conductivity properties. The water uptake and ion conductivity were increased until 9 wt% $SiO_2$, and then decreased. The maximum proton conductivity equal to $52mScm^{-1}$ was measured for the BPSFBC/$SiO_2$-9 composite membrane at $90^{\circ}C$ and 100% relative humidity. From the measured results, it is distinct that the manufactured composite membrane BPSFBC/$SiO_2$-9 can be considered as a polymer membrane suitable for a fuel cell electrolyte.

• Korean Chemical Engineering Research
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• 제49권1호
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• pp.69-74
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• 2011

본 연구에서는 전기방사를 이용하여 PS의 취성을 개선하고자 내충격성이 우수한 HIPS와 가교제 DVB를 혼합하여 방사한 후 가교와 술폰화 반응을 통해 HIPS 이온교환 나노섬유를 제조하였다. 또한, 이들을 FT-IR, XPS, 함수율, 이온교환 용량, SEM 및 접촉각으로 나노섬유의 기본특성을 확인하였다. FT-IR 및 XPS 구조분석 결과, HIPS 나노섬유는 술폰화 시간이 지남에 따라 $-SO_3H$기의 도입이 증가하는 것을 확인할 수 있었다. 또한 술폰화 시간이 지남에 따라 섬유의 친수성이 증가하여 DVB 함량이 7.5 wt%, 술폰화 시간이 200분일 때 함수율과 이온교환용량은 최대 75.6%, 2.67 meq/g으로 나타났다.

• Biomolecules & Therapeutics
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• 제16권3호
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• pp.219-225
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• 2008

Taurine is the most abundant amino acid in many tissues and is found to be enhancing the bone tissue formation or inhibits the bone loss. Although it is reported that taurine reduces the alveolar bone loss through inhibiting the bone resorption, its functions of taurine and expression of taurine transporter (TauT) in bone have not been identified yet. The purpose of this study is to clarify the uptake mechanism of taurine in osteoblast using mouse osteoblast cell lines. In this study, mouse stromal ST2 cells and mouse osteoblast-like MC3T3-E1 cells as osteoblast cell lines were used. The activity of taurine uptake was assessed by measuring the uptake of [$^3H$]taurine in the presence or absence of inhibitors. TauT mRNA was detected in ST2 and MC3T3-E1 cells. [$^3H$]Taurine uptake by these cells was dependent on the presence of extracellular calcium ion. The [$^3H$]taurine uptake in ST2 cells treated with 4 mM calcium was increased by 1.7-fold of the control which was a significant change. In contrast, in $Ca^{++}$-free condition and L-type calcium channel blockers (CCBs), taurine transport to osteocyte was significantly inhibited. In oxidative stress conditions, [$^3H$]taurine uptake was decreased by TNF-$\alpha$ and $H_2O_2$. Under the hyperosmotic conditions, taurine uptake was increased, but inhibited by CCBs in hyperosmotic condition. These results suggest that, in mouse osteoblast cell lines, taurine uptake by TauT was increased by the presence of extracellular calcium, whereas decreased by CCBs and oxidative stresses, such as TNF-$\alpha$ and $H_2O_2$.