• Toxicological Research
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• v.14 no.3
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• pp.459-463
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• 1998

The antigenicity of intralipidos was investigated in guinea pig, mice and rats. Antigenicity tests-active systemic anaphylaxis (ASA), passive systemic anaphylaxis (PSA), passive cutaneous anaphylaxix (PCA) of this materials were performed. The results were followed: 1. After sensitizaion with YPL, YPL+intralipidos, and intralipidos, emulsified with complete Freund's adjuvant (CFA), guinea pigs didn's show any anaphylatic shock symptom in the ASA test, 2. These materials didn't show any anaphylatic shock symptom in the PSA test, 3. After sensitization with antisera of YPL, YPL+intralipidos, and intralipidos sensitized mice, blue spots were not observed on the hypodermis of back of rats in the PCA test. From the results of this investigation, the antigenicity of YPL, intralipidos was negative under the present experimental condition.

• Toxicological Research
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• v.14 no.3
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• pp.453-457
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• 1998

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

• Toxicological Research
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• v.14 no.3
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• pp.443-452
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• 1998

This sutdy was carried out to investigate the acute toxicity and foru-week intravenous toxicity of the intralipidos in rats and rabbits. The acute toxicity study of Intralipidos was performed in Spragur-Dawley (SD) rats. Intralipidos was administered by intravenous to maximum dose 200 ml/kg. $LD_{50}$ of intralipidos was found 139.5ml/kg and 153.8ml/kg in male female SD rats. Four-week toxicity of intralipidos using New Zealand White Rabbit and SD rats. The Rabbit and Rats were administered by intravenous seven days per week for 28 days, with dosage of 15, 6, 2 ml/kg/day and 20, 6, 2ml/kg/day, respectively. Animals treated with intralipidos did not cause any death and show any clinical signs. They did not show any significant changes of body weight, feed uptake and water consumption. They were not significantly different from the control group in urinalysis, ocular examination hematological, serum biochemical value and histopathological examination. Therefore, Intralipidos was not indicated to have any toxic effect in the Rabbits and Rats, when it was administrated by intravenous below the dosage 15ml/kg/day and 20 ml/kg/day for four weeks.