• Toxicological Research
• /
• 제34권4호
• /
• pp.325-332
• /
• 2018

The mechanism of the previously reported antidiabetic effect of Basella alba is unknown. This study investigated the role of B. alba aqueous leaf extract in the modulation of inflammatory cytokines and islet morphology in streptozotocin-induced diabetic rats. Forty male Wistar rats, between 8 and 10 weeks old, were randomly divided into four groups (n = 10) and administered the following treatments: Healthy control (H-c) and Diabetic control (D-c) animals received normal saline 0.5 mL/100 g body weight daily, while Healthy Treatment (H-Ba) and Diabetic Treatment (D-Ba) rats received the plant extract 200 mg/kg body weight daily. All treatments were administered by oral gavage. Diabetes was induced in D-c and D-Ba rats by a single intraperitoneal injection of streptozotocin (55 mg/kg body). The body weight and fasting blood sugar (FBS) levels were recorded every week for 4 weeks, after which the rats were euthanized and samples collected for further analysis. After the experiment, FBS level was significantly reduced (p < 0.0001) in rats in the D-Ba group, but increased (p < 0.001) in rats in the D-c group. The absolute (H-c and H-Ba vs D-c, p < 0.05) and relative (D-Ba vs H-c, p < 0.05; D-Ba vs H-Ba, p < 0.005) weights of the pancreases were significantly higher after the experiment. The rats in the D-c group had significantly higher levels of serum interleukin-$1{\beta}$ (p < 0.001 vs H-c; p < 0.05 vs H-Ba and D-Ba) and monocyte chemotactic protein-1 (p < 0.0001), but lower levels of interleukin-10 (p < 0.05) in comparison with the other groups. Histopathological examination revealed severe interstitial congestion, reduced islet area (p < 0.0001), and increased islet cell density in the D-c group compared with those in the D-Ba group. From these findings, it was concluded that the aqueous extract of B. alba stimulates the recovery of beta-islet morphology in streptozotocininduced diabetic rats by modulating the peripheral production of inflammatory cytokines.

• 한국임상수의학회지
• /
• 제28권4호
• /
• pp.403-407
• /
• 2011

A form of polycystic ovary (PCO) resembling some aspects of the human PCO syndrome (PCOS) can be induced in rats by a single injection of estradiol valerate (EV). An increase in sympathetic outflow to the ovary precedes, by several weeks, the appearance of cysts, suggesting the involvement of a neurogenic component in the pathology of this ovarian dysfunction. To test the hypotheses that the change in sympathetic tone is related to an augmented production of hippocampal and/or ovarian nerve growth factor (NGF), and that this abnormally elevated production of NGF contributes to the induction of PCOS induced by EV. The animals were sacrificed after PCOS induction and the ovaries and hippocampus were sectioned and compared to the normal control. The expression of NGF was measured by immunohistochemical staining and Western blot analysis in the ovaries and hippocampus. EV-induced PCOS showed significant increase of ovarian NGF expression. Immunohistochemical expression of NGF was confined to the follicular cells and interstitial cells. Hippocampal NGF expression was not significantly changed. In conclusion EV-induced PCOS was related to the ovarian sympathetic activation which was mediated by NGF.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제31권5호
• /
• pp.379-389
• /
• 2005

Obstructive adenitis of the salivary gland following salivary stone or infectious disease of the gland and surrounding tissues is a common disease. It is often difficult to decide whether to perform total excision of the gland or to consider conservative treatment. The present study was designed to investigate histological, histochemical changes of submandibular gland after ligating the excretory duct for identifying the results of gland duct obstruction. A group of 40 rat of Sprague-Dawley weighing about $200{\sim}220gm$ were used in the present study. 30 rats had ligation of the main excretory ducts of submandibular glands just at the exit from the glands. For controls, 10 rats had a sham operation without duct ligation. They were inducted into euthanagia state by intracardial Ketamine injection in 1, 2, 3, 5, 7, 14, 21, 28, 35 and 42 days after the ligation. In each ligation period, 3 animals were used for ligation and one animal was for control. The submandibular glands were dissected out at sacrifice and stained with H&E, PAS, mucicarmine stain and histological examination were carried out under the light microscope. After examination and comparison of all specimens, the results were as follows: 1. In the features of H&E stain, acini disappeared by degrees after the ligation of the excretary duct and interstitial cells were displaced into fibrous connective tissue. Salivary gland had been atrophied with enlarging ducts and proliferating ductal cells. 2. Through total experimental period, a lot of vessels were observed and the atrophy of serous gland was severer than that of mucous gland. 3. The deep portion of submandibular glands showed severe degeneration rather than superficial portion of them after the ligation. 4. The changes which had enlarged ducts and proliferating ductal cells were observed in entire gland and more prominent in serous gland than mucous gland after the ligation. 5. Although PAS and mucicarmine reactions were decreased gradually after the ligation with the lapse of time, since 2 to 3 weeks they were strong positive reactions on entire gland, especially on duct-like structure. So, we can suggest that salivary gland will be atrophied but, survived acini will be redistributed around the ducts.

• 대한치과교정학회지
• /
• 제40권4호
• /
• pp.227-238
• /
• 2010

저신장이란 같은 연령 및 성별 소아들의 표준 신장 평균치에서 -2.0 SD 이하인 경우를 말한다. 본 연구의 목적은 저신장 소아의 두개안면골격의 특성을 분석하고, 성장호르몬 치료가 신장을 성장시킴과 동시에 두개안면골격에 어떤 영향을 주는지 알아보고자 함이다. 영남대학교 의과대학 부속병원 소아청소년과에서 저신장으로 진단받은 소아를 대상으로 성장 호르몬 치료 전 그리고 치료 후 1년, 2년 후 총 3회 측모 두부 방사선 규격 사진의 촬영을 시행하였다. 대조군은 경북대학교 치의학전문대학원 교정과에 소장된 한국인 평균 신장의 2 표준오차 내의 아동들을 대상으로 측모 두부 방사선 규격사진을 2년마다 10년간 촬영한 자료를 이용하여 저신장 소아의 연령과 성별을 기준으로 짝진 표집(paired sampling)을 하였다. 성장 호르몬 치료 전 저신장 소아는 작고 후퇴된 하악골과 편평한 두개저를 가지는 것으로 나타났다. 성장 호르몬 치료 후 anterior, posterior cranial base length, upper posterior facial height, lower anterior facial height, posterior total facial height, mandibular ramus length, mandibular corpus length와 overall mandibular length 성장량이 정상군에서의 2년 성장량과 비교했을 때 유의하게 큰 것으로 나타났으며 각도 계측에서는 saddle angle, mandibular plane angle과 ANB변화량이 정상군에서의 2년 성장량과 비교했을 때 큰 변화를 보이며 정상군의 평균치를 따라잡는 경향을 보였다. 성장 호르몬 치료는 불균형적인 성장의 징후 없이 정상화를 향한 성장(따라잡기 성장)을 나타내었으며 이는 특히 하악과두의 성장과 후안면고경의 성장을 촉진시켜 저신장 소아의 convex한 profile을 완화하는 것으로 판단된다.

• Nuclear Engineering and Technology
• /
• 제31권3호
• /
• pp.335-343
• /
• 1999

The effects of N $b_2$ $O_{5}$ and oxygen potential on the densification and grain growth of U $O_2$ fuel have been investigated.0.3 wt% N $b_2$ $O_{5}$ -doped U $O_2$fuel pellets were sintered at 1$700^{\circ}C$ for 4 hours in sintering atmospheres which have various ratios of $H_2O$ to $H_2$ gas. Compared with those of undoped U $O_2$ pellets, the sintered density and grain size of the 0.3 wt% N $b_2$ $O_{5}$ -doped U $O_2$ pellet increase under the $H_2O$/ $H_2$ gas ratio of 5.0$\times$10$^{-3}$ to 1.0$\times$10$^{-2}$ and under the $H_2O$/ $H_2$gas ratio of 5.0$\times$10$^{-3}$ to $1.5\times$10$^{-2}$ , respectively. The sintering of U $O_2$fuel pellets containing 0.1 wt% to 0.5 wt% N $b_2$ $O_{5}$ was carried out at 168$0^{\circ}C$ for 4 hours. The enhancing effect of N $b_2$ $O_{5}$ on the sintered density and grain size becomes larger as the N $b_2$ $O_{5}$ content increases. The solubility limit of N $b_2$ $O_{5}$ in U $O_{2}$ seems to be between 0.3 wt% and 0.5 wt%, and beyond the solubility limit the second phase whose composition corresponds near to N $b_2$U $O_{6}$ is precipitated on grain boundary. The enhancement of densification and grain growth in U $O_2$ is attributed to the increased concentration of a uranium vacancy which is formed by the interstitial N $b^{4+}$ ion in the U $O_2$ lattice.

• 한국건설순환자원학회논문집
• /
• 제5권1호
• /
• pp.21-28
• /
• 2017

최근 원자력 발전소 및 LNG 탱크의 외벽을 제작하는데 있어 강판 콘크리트(steel-plate concrete, SC)와 같은 합성형 구조모듈(steel-plate concrete panel, SCP)의 사용이 증가하고 있다. SCP는 공장에서 생산 한 후 이송되므로, 이송에 드는 비용을 줄이기 위해 경량화가 필수적이다. 이때 경량골재 콘크리트를 사용해 SCP의 내부를 채워 밀도를 낮출 수 있지만, 현재 콘크리트 배합법은 콘크리트의 품질을 확보하기 위해 경량골재의 사용량이 제한되어 경량화에 한계가 있다. 본 연구에서 제안하는 프리플레이스트 경량 콘크리트 배합법은 거푸집 내 경량골재를 먼저 채운 후(pre-packing) 그라우트를 주입하는 공법으로, 콘크리트의 품질확보 및 밀도를 낮출 수 있다. 다양한 종류의 경량골재 및 그라우트 배합을 사용해 실험을 수행하였고, 밀도 $1,600kg/m^3$, 압축강도 30MPa급 프리플레이스트 경량 콘크리트 배합비를 선정하여 SCP 시편을 제작하였다. 제작된 SCP 시편의 구조성능 시험을 위해 3점 휨 실험을 수행하였다. 고유동 콘크리트를 사용해 SCP의 내부를 채운 시편과 비교해 프리플레이스트 경량 콘크리트를 사용해 제작된 SCP 시편은 비슷한 수준의 휨 저항 성능을 보였으며 밀도가 30% 이상 낮았다. 따라서 프리플레이스트 경량 콘크리트를 사용해한 SCP 제작 시, 기존의 SCP와 비슷한 수준의 구조성능을 가지며 밀도를 낮춰 이송 비용을 감축할 수 있을 것으로 기대된다.

• BMB Reports
• /
• 제38권6호
• /
• pp.739-747
• /
• 2005

Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

• 한국해양학회지
• /
• 제22권1호
• /
• pp.1-8
• /
• 1987

신양리층은 제주도 성산반도 주변에 발달된 층으로 주로 화산쇄설물, 현무암 역, 패각편으로 이루어져 있으며, 퇴적구조, 주직 및 퇴적물의 성분을 중심으로 암상 을 분류한 결과위로 가면서 평행층리 사암상(I), 역암상(II), 사층리 사암상(III)으 로 구분되었다. (암상 I)은 조립질 박층과 세립질 박층이 규칙적으로 반복 교호하 는 쇄기모양 및 평행층리를 보여주며 이는 foreshore 퇴적환경의 특징적인 퇴적구조 이다. 본 암상의 입자들을 다른 두 암상에 비해 양호한 원마도와 분급도를 보이며 부분적으로 역분급층리를 보인다. (암상 II)는 clast-supported하고 매우 불량한 분 급도의 현무암 역으로 이루어져 있으며 신앙리해변을 따라 연속되어 노출되어 있 다. 역 사이의 공간을 주로 화산쇄설물과 원마도가 양호한 소량의 패각쇄설물로 채 워져 있다. 현무암 역의 크기 감소뿐만 아니라 최대 층후도 남쪽(신양리)으로 가면 서 줄어든다. 이 암상 하부에 는 직 하부의 (암상 I)로부터 기원한 커다란 판상의 역이층리면에 평행하게 누워 있으며 역의 크기는 다양하고 불규칙하며 어떤 지역적 인 방향성을 보이지 않는다. 이는 원지(in-situ) 침식잔류 역으로 판단된다. 따라서 현무암역은 해수면의 상대적인 상승으로 인한 해빈잔류역으로 이역들을 부분적으로 연안류와 파랑에너지의 영향을 받았을 것으로 판단된다. (암상 II)의 상부에 발달한 남쪽으로 경사하는 사층리는 연안류의 존재와 방향을 알려준다. (암상 III)은 세립내 지 중립사 크기의 주로 화산쇄설물로 이루어진 암상으로 상부 내해안(upper shoref ace)의 특징인 육지와 바다쪽으로 경사하는 bidirectional trough cross-stratification 과 파랑물결구조가 나타난다. 전반적으로 신양리층은 foreshore에서 상부내해안에 이르는 환경에서 퇴적되었으며 (암상 I)과 (암상 II)사이에는 소규모의 부정합이 존재한다.

• 한국발생생물학회지:발생과생식
• /
• 제15권3호
• /
• pp.249-256
• /
• 2011

Nesfatin-1/NUCB2, which is secreted from the brain, is known to control appetite and energy metabolism. Recent studies have been shown that nesfatin-1/NUCB2 was expressed not only in the brain, but it was also expressed in the gastric organs and adipose tissue. However, little is known about the expression of nesfatin-1/NUCB2 in the male reproductive system. Therefore, we examined whether the nesfatin-1/NUCB2 and its binding site exists in the male reproductive organs. Nesfatin-1/NUCB2 mRNA and protein were detected in the mouse testis and epididymis by PCR and Western blot analysis. As a result of the immunohistochemistry staining, the nesfatin-1 protein was localized at the interstitial cells and Leydig cells in the testis. Nesfatin-1 binding sites were also displayed at boundary cells in the tunica albuginea. Furthermore, in order to examine if the expression of nesfatin-1/NUCB2 mRNA in the testis and epididymis were affected by gonadotropin, its mRNA expression was analyzed after PMSG administration into mice. NUCB2 mRNA expression levels were increased in both of the testis and epididymis after PMSG administration. These results demonstrated for the first time that nesfatin-1 and its binding site were expressed in the mouse testis and epididymis. In addition, nesfatin-1/NUCB2 mRNA expression was controlled by gonadotropin, suggesting a possible role of nesfatin-1 in the male reproductive organs as a local regulator. Due to this, further study is needed to elucidate the functions of nesfatin-1 on the male reproductive system.

• 한국가축번식학회지
• /
• 제9권2호
• /
• pp.124-132
• /
• 1985

The work was conducted to clarify the development of testes and histological changes of the Seminiferous tubules that is the Surface area, diameter, length of the tubules and the process of Spermatic cells growth in Seminal epithelium in relation to Sexual maturity of the meat-type cockerels. In addition the present experiments were planned to gain some basic data helpful for the study of reproductive physiology in meat-type cockerels and also to compare the histological changes Seminiferous tubules between egg and general purpose type cockerels. 1. The growth pattern of body weight, testes and comb was classified into four phase according to age. Body weight increased up to about 2kg at 2nd stage and to 4.3kg at 4th stage while at 3rd stage the growth rate of body weight was slower. The tests developed slowly until 22 weeks of age with no correlation to the growth of body weight. However, it grew rapidly afterwards, reaching 27.79$\pm$9.26gm and 42.46$\pm$5.33 gm at 24 and 28 weeks of age, respectively. The spermatozoa was seen first at 24 weeks of age. On the other hand, comb grew at similar rate to the testes, weighing on the average 21.78$\pm$6.48gm and 41.42$\pm$1.25gm at 24 and 28 weeks of age, respectively. 2. The areal rate of the Seminiferous tubules to interstitial tissues was 66%:34% at 2 weeks of age and the rate of the former increased in comparison to that of the latter as they grew older, becoming 94%:7% between at 24 weeks of age, when the testes weight increased rapidly too. 3. Diameter of Seminiferous tubules at 2 weeks of age was 42.76$\pm$1.04$\mu$m and then it gradually increased as the testes grew until 22 weeks of age, when it grew rapidly, increasing about 2.9 times of those of 2 weeks and at last increased up to 5 times at 24 weeks. 4. Length of Seminiferous tubules was 4.5cm at 2 weeks and then it was gradually increased. At 22 weeks old the length of tubules was icnreased rapidly being 321 meter and increased more than double, being 658 meter at 24 weeks old. 5. The cells of Seminal epithelium developed poorly and the number of cells were relatively few until 16 weeks old. Afterwards the cells increased rapidly in number and at 18 weeks, the number became twice of those of 16 weeks, reaching 140.43$\pm$43.6. At 24 weeks the cells increased more rapidly, reaching to 10 layers and Spermatozoa were first time seen.