Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Expression of Nesfatin-1/NUCB2 and Its Binding Site in Mouse Testis and Epididymis

  • Kim, Jin-Hee (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Kim, Hyun-Sook (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Kim, Su-Min (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Yang, Hye-Jin (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Cho, Hyun-Hae (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Hwang, Sup-Yong (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University) ;
  • Moon, Chan-Il (Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Yang, Hyun-Won (Dept. of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
  • Received : 2011.07.29
  • Accepted : 2011.08.14
  • Published : 2011.09.30
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Abstract

Nesfatin-1/NUCB2, which is secreted from the brain, is known to control appetite and energy metabolism. Recent studies have been shown that nesfatin-1/NUCB2 was expressed not only in the brain, but it was also expressed in the gastric organs and adipose tissue. However, little is known about the expression of nesfatin-1/NUCB2 in the male reproductive system. Therefore, we examined whether the nesfatin-1/NUCB2 and its binding site exists in the male reproductive organs. Nesfatin-1/NUCB2 mRNA and protein were detected in the mouse testis and epididymis by PCR and Western blot analysis. As a result of the immunohistochemistry staining, the nesfatin-1 protein was localized at the interstitial cells and Leydig cells in the testis. Nesfatin-1 binding sites were also displayed at boundary cells in the tunica albuginea. Furthermore, in order to examine if the expression of nesfatin-1/NUCB2 mRNA in the testis and epididymis were affected by gonadotropin, its mRNA expression was analyzed after PMSG administration into mice. NUCB2 mRNA expression levels were increased in both of the testis and epididymis after PMSG administration. These results demonstrated for the first time that nesfatin-1 and its binding site were expressed in the mouse testis and epididymis. In addition, nesfatin-1/NUCB2 mRNA expression was controlled by gonadotropin, suggesting a possible role of nesfatin-1 in the male reproductive organs as a local regulator. Due to this, further study is needed to elucidate the functions of nesfatin-1 on the male reproductive system.

Keywords

References

  1. Akingbemi BT, Sottas CM, Koulova AI, Klinefelter GR, Hardy MP (2004) Inhibition of testicular steroidogenesis by the xenoestrogen bisphenol A is associated with reduced pituitary luteinizing hormone secretion and decreased steroidogenic enzyme gene expression in rat Leydig cells. Endocrinology 145:592-603. https://doi.org/10.1210/en.2003-1174
  2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (1994) Human protein NEFA, a novel DNA binding/ EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. Biol Chem Hoppe Seyler 375:497-512. https://doi.org/10.1515/bchm3.1994.375.8.497
  3. Brailoiu GC, Dun SL, Brailoiu E, Inan S, Yang J, Chang JK, Dun NJ (2007) Nesfatin-1: distribution and interaction with a G protein coupled receptor in the rat brain. Endocrinology 148:5088-5094. https://doi.org/10.1210/en.2007-0701
  4. Dufau ML (1988) Endocrine regulation and communicating functions of the Leydig cell. Annu Rev Physiol 50:483-508. https://doi.org/10.1146/annurev.ph.50.030188.002411
  5. Foo KS, Brauner H, Ostenson CG, Broberger C (2010) Nucleobindin-2/nesfatin in the endocrine pancreas: distribution and relationship to glycaemic state. J Endocrinol 204: 255-263. https://doi.org/10.1677/JOE-09-0254
  6. Foo KS, Brismar H, Broberger C (2008) Distribution and neuropeptide coexistence of nucleobindin-2 mRNA/nesfatinlike immunoreactivityin the rat CNS. Neuroscience 156:563-579. https://doi.org/10.1016/j.neuroscience.2008.07.054
  7. Fort P, Salvert D, Hanriot L, Jego S, Shimizu H, Hashimoto K, Mori M, Luppi PH (2008) The satiety molecule nesfatin-1 is co-expressed with melanin concentrating hormone in tuberal hypothalamic neurons of the rat. Neuroscience 155: 174-181. https://doi.org/10.1016/j.neuroscience.2008.05.035
  8. Garcia-Galiano D, Navarro VM, Gaytan F, Tena-Sempere M (2010) Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J Mol Endocrinol 45:281-290. https://doi.org/10.1677/JME-10-0059
  9. Goebel M, Stengel A, Wang L, Lambrecht NW, Taché Y (2009) Nesfatin-1 immunoreactivity in rat brain and spinal cord autonomic nuclei. Neurosci Lett 452:241-246. https://doi.org/10.1016/j.neulet.2009.01.064
  10. Gonzalez R, Tiwari A, Unniappan S (2009) Pancreatic beta cells colocalize insulin and pronesfatin immunoreactivity in rodents. Biochem Biophys Res Commun 381:643-648. https://doi.org/10.1016/j.bbrc.2009.02.104
  11. Kanai Y, Tanuma S (1992) Purification of a novel B cell growth and differentiation factor associated with lupus syndrome. Immunol Lett 32:43-48. https://doi.org/10.1016/0165-2478(92)90197-V
  12. Kohno D, Nakata M, Maejima Y, Shimizu H, Sedbazar U, Yoshida N, Dezaki K, Onaka T, Mori M, Yada T (2008) Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding. Endocrinology 149:1295-1301. https://doi.org/10.1210/en.2007-1276
  13. Kukucka Mark A, Misra Hara P (1992) HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate. Arch Androl 29: 185-90. https://doi.org/10.3109/01485019208987723
  14. Miura K, Titani K, Kurosawa Y, Kanai Y (1992) Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure. Biochem Biophys Res Commun 187:375-380. https://doi.org/10.1016/S0006-291X(05)81503-7
  15. Oh-I S, Shimizu H, Satoh T, Okada S, Adachi S, Inoue K, Eguchi H, Yamamoto M, Imaki T, Hashimoto K, Tsuchiya T, Monden T, Horiguchi K, Yamada M, Mori M (2006) Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 443:709-712. https://doi.org/10.1038/nature05162
  16. Ramanjaneya M, Chen J, Brown JE, Tripathi G, Hallschmid M, Patel S, Kern W, Hillhouse EW, Lehnert H, Tan BK et al. (2010) Identification of nesfatin-1 in human and murine adipose tissue: a novel depot specific adipokine with increased levels in obesity. Endocrinology 151:3169-3180. https://doi.org/10.1210/en.2009-1358
  17. Shimizu H, Oh-I S, Hashimoto K, Nakata M, Yamamoto S, Yoshida N, Eguchi H, Kato I, Inoue K, Satoh T, Okada S, Yamada M, Yada T, Mori M (2009) Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism. Endocrinology 150:662-671. https://doi.org/10.1210/en.2008-0598
  18. Stengel A, Goebel M, Wang L, Tache Y (2009a) Ghrelin, des-acyl ghrelin and nesfatin-1 in gastric X/A-like cells: Role as regulators of food intake and body weight. Peptides 31:357-369.
  19. Stengel A, Goebel M, Yakubov I, Wang L, Witcher D, Coskun T, Tache Y, Sachs G, Lambrecht NW (2009b) Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa. Endocrinology 150:232-238. https://doi.org/10.1210/en.2008-0747
  20. Stengel A, Tache Y (2011) Minireview: Nesfatin-1-An Emerging New Player in the Brain-Gut, Endocrine, and Metabolic Axis. Endocrinology 152:Epub ahead of print.
  21. Zhang AQ, Li XL, Jiang CY, Lin L, Shi RH, Chen JD, Oomura Y (2010) Expression of nesfatin-1/NUCB2 in rodent digestive system. World J Gastroenterol 16:1735-1741. https://doi.org/10.3748/wjg.v16.i14.1735