• Proceedings of the Korean Society of Crop Science Conference
• /
• 1994.10a
• /
• pp.20-21
• /
• 1994

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.8 no.2
• /
• pp.123-127
• /
• 1988

Even though grass species are one of major importance in livestock industry, the response of intergeneric hybridization of Lolium and Festuca and interspecific hybridization of Lolium m.X Lolium p. to Korean climatic condition has not been adequately documented. This reasearch was conducted to examine the growth characteristics and dry matter yield of Festulolium, cv. Felopa and C-Stamm N 66 and hybrid ryegrass, cv. Maja and Tetilelite. 1. The early growth of Italian ryegrass was better than that of hybrid ryegrass and Festulolium. The plant length and height of Italian ryegrass were longer/taller than those of hybrid ryegrass and Festulolium, but the number of tillers of Italian ryegrass was less than that of hybrid ryegrass and Festulolium. 2. Festuloliu and hybrid ryegrass were more tolerant than Italian ryegrass to winter killing, 70-80% of tillers was survived. 3. After wintering, early spring growth of hybrid ryegrass and Festulolium were better than that of Italian ryegrass. Dry matter yield of hybrid ryegrass and Festulolium were more than that of Italian ryegrass. 4. Spikes of hybrid ryegrass and Festulolium (C-Stamm N66) were more than those of Italian ryegrass. Seed yield per $m^2$ of hybrid ryegrass and Italian ryegrass were almost same, but that of Festuloliu was lower.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.8 no.3
• /
• pp.123-127
• /
• 1988

Even though grass species are one of major importance in livestock industry, the response of intergeneric hybridization of Lolium and Festuca and interspecific hybridization of Lolium m.X Lolium p. to Korean climatic condition has not been adequately documented. This reasearch was conducted to examine the growth characteristics and dry matter yield of Festulolium, cv. Felopa and C-Stamm N 66 and hybrid ryegrass, cv. Maja and Tetilelite. 1. The early growth of Italian ryegrass was better than that of hybrid ryegrass and Festulolium. The plant length and helght of Italian ryegrass were longerltaller than those of hybrid ryegrass and Festulolium, but the number of tillers of Italian ryegrass was less than that of hybrid ryegrass and Festulolium. 2. Festulolium and hybrid ryegrass were more tolerant than Italian xyegrass to winter killing, 70-80% of tillers was survived. 3. After wintering, early spring growth of hybrid ryegrass and Festulolium were better than that of Italian ryegrass. Dry matter yield of hybrid ryegrass and Festulolium were more than that of Italian ryegrass. 4. Spikes of hybrid ryegrass and Festulolium (C-Stamm N66) were more than those of Italian ryegrass. Seed yield per $m^2$ of hybrid ryegrass and Italian ryegrass were almost same, but that of Festulolium was lower.

• Korean journal of applied entomology
• /
• v.58 no.4
• /
• pp.305-311
• /
• 2019

Artificial interspecific matings between Matsumuraeses phaseoli and M. falcana (Lepidoptera: Tortricidae) were conducted to know the possibility of hybridization of the two sympatric species. Reciprocal crossings successfully produced F1 hybrids. Most of F2 crosses yielded progenies except all mating trials with females of F1 hybrid obtained from M. phaseoli female. Inbreedings of the F2 hybrids produced F3 progenies. In backcrossings between F1 hybrids and parent lines, all the two mating trials with females of F1 hybrid obtained from M. phaseoli females did not produce any progeny, while other 6 backcrosses produced the next generations. Inbreedings of the backcross lines also produced subsequent progenies. These results indicated that F1 females produced from hybridization between M. phaseoli females and M. falcana males were sterile. Conclusively, it suggested that a partial reproductive isolation at a postzygotic stage can occur between the two sympatric species.

• Horticultural Science & Technology
• /
• v.33 no.3
• /
• pp.396-402
• /
• 2015

This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 ${\times}$ 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 ${\times}$ 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.

• Journal of Forest and Environmental Science
• /
• v.33 no.3
• /
• pp.197-201
• /
• 2017

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

• Journal of the Korean Society of Tobacco Science
• /
• v.17 no.2
• /
• pp.109-113
• /
• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• Plant Resources
• /
• v.7 no.2
• /
• pp.87-92
• /
• 2004

Five different poplar hybrids were tested for rescuing embryo to elongate in vitro plantiets after hybridization. Ovaries and ovules were cultured on Woody Plant Medium (WPM) supplemented with cytokinins, 6-benzylamine (BA) and zeatin. Multiple shoots were initiated from half section of capsule with immature embryos after 21 days from pollination and tiny shoots were formed after the expansion of cotyledons in ovule cultures. Germinating response was better in intraspecific hybrids $(6.53\pm1.66)$ than interspecific crosses $(0.93\pm0.54)$ from half section of capsules on WPM medium. In general, zeatin was better than BA in inducing multiple shoots from isolated ovules. The highest average number $(19.40\pm4.53)$ of shoots was produced from immature ovules of 21 days post-pollination of WPM medium supplemented with 5.0 mg/L zeatin. The highest percentage of germination was 93% from the half section of in vitro ovary cultures. Soil acclimation was successfully conducted in cell tray containing artificially mixed soil with 96% survival rate.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.1
• /
• pp.31-37
• /
• 2003

Identification of compatible parental line is of great importance in introduction of useful characters to onion breeding program, beyond the severe hybridization barrier. Phylogenic analysis of Allium section Cepa was conducted through PCR by URPs, repeated sequences of A. fistulosum, and microsatellite markers. Totally 76 accessions originated from 21 countries were clustered into five groups at a 0.84-similarity level: group I;A. cepa and its wild relatives and A. cepa ssp. ascalonicum, group II; A. cepa ssp. wakegii, A. cepa ssp. proliferum and Samcheung-pa group III; A. fistulosum and A. altaicum, group IV; A. galanthum, group V; Soeckkori-pa. Samcheung-pa and Soekkori-pa, Korean local varieties, shared band type of both Cepa group and Altaicum group, indicating that those are derived from interspecific hybridization between A. fistulosum and A. cepa.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.55 no.2
• /
• pp.137-145
• /
• 2022

This study was conducted to identify the taxonomic differences in the characteristics of Rhodeus ocellatus and Acheilognathus yamatsutae during their early life history via an interspecific hybridization experiment. Results showed that the hatching rates of the cross-bred eggs were 60% for cross OY (R. ocellatus ♀×A. yamatsutae ♂) and 70% for cross YO (A. yamatsutae ♀×R. ocellatus ♂). The dorsal fin rays of YO were similar to those of R. ocellatus. The hybridization test of A. yamatsutae and R. ocellatus living in the same water stream revealed that no interspecies reproductive isolation occurred. OY and YO showed similar maternal and paternal characteristic. The juvenile of OY did not resemble both paternal and maternal fin rays, indicating a difference. The hatching larvae of the hybrids showed the morphological characteristics of their parents but varied in terms of the shapes of their parts.