Genetic Distance of Allium Section Cepa by DNA Fingerprint

  • Kim, Haeng-Hoon (National Institute of Agricultural Biotechnology) ;
  • Cho, Eun-Gi (National Institute of Agricultural Biotechnology) ;
  • Baek, Hyung-Jin (National Institute of Agricultural Biotechnology) ;
  • Kim, Chang-Yung (National Institute of Agricultural Biotechnology) ;
  • Chae, Young-Am (Department of Agronomy and Research Center for New Biomaterials in Agriculture, Seoul National University)
  • Published : 2003.03.01

Abstract

Identification of compatible parental line is of great importance in introduction of useful characters to onion breeding program, beyond the severe hybridization barrier. Phylogenic analysis of Allium section Cepa was conducted through PCR by URPs, repeated sequences of A. fistulosum, and microsatellite markers. Totally 76 accessions originated from 21 countries were clustered into five groups at a 0.84-similarity level: group I;A. cepa and its wild relatives and A. cepa ssp. ascalonicum, group II; A. cepa ssp. wakegii, A. cepa ssp. proliferum and Samcheung-pa group III; A. fistulosum and A. altaicum, group IV; A. galanthum, group V; Soeckkori-pa. Samcheung-pa and Soekkori-pa, Korean local varieties, shared band type of both Cepa group and Altaicum group, indicating that those are derived from interspecific hybridization between A. fistulosum and A. cepa.

Keywords

References

  1. Bames, S. R., A. M. James, and G. Jamieson. 1985. The organization, nucleotide sequence, and chromosomal distribution of a satellite DNA from Allium cepa. Chromosoma 92 : 185-192 https://doi.org/10.1007/BF00348692
  2. Bradeen, J. M. and M. J. Havey. 1995. Restriction fragment length polymorphisms reveal considerable nuclear divergence within a well-supported maternal clade in Allium section Cepa (Alliaceae). American J. of Botany 82(11) : 1455-1462 https://doi.org/10.2307/2445873
  3. Buiteveld, J., Y. Suo, M. M. van Lookeren Campagne, and J.Creemers Molenaar. 1998. Production and characterization of somatic hybrid plants between leek (Allium ampeloprasum L.)and onion (Allium cepa L.). Theor. Appl. Genet. 96 : 765-775 https://doi.org/10.1007/s001220050800
  4. Dubouzet, J. G., K. Shinoda, and N. Murata. 1997. Phylogeny of Allium L. subgenus rhizirideum (G. Don ex Koch) Wendelbo according to dot blot hybridization with randomly amplified DNA probes. Theor. Appl. Genet. 95 : 1223-1228 https://doi.org/10.1007/s001220050685
  5. Fischer, D. and K. Bachmann. 2000. Onion microsatellites for germplasm analysis and their use in accessing intra- and interspecific relatedness within the subgenus Rhizirideum. Theor. Appl.Genet. 101 : 153-164 https://doi.org/10.1007/s001220051464
  6. Friesen, N. and M. Klaas. 1998. Origin of some minor vegetatively propagated Allium crops studied with RAPD and GISH.Genetic Resources and Crop Evolution 45 : 511-523 https://doi.org/10.1023/A:1008647700251
  7. Friesen, N. and S. Pollner. 1999. RAPD and noncoding chloroplast DNA reveal a single origin of the cultivated Allium fistulosum from A. altaicum (Alliaceae). American. J. Botany 86(4): 554-562 https://doi.org/10.2307/2656817
  8. Hanelt, P. 1990. Taxonomy, evolution and history. In: H. Rabinowitch and J. Brewster (ed). Onions and Allied Crops. vol. 1 Botany, Physiology and genetics. CRC Press, Boca Raton, Florida.pp: 2-26
  9. Hanelt P., J. Schultze-Montel, R. Fritsch, J. Kruse, H. Maab, H.Ohle, and K. Pistrick. 1992. Infrageneric grouping of Allium the Gatersleben approach. In: P. Hanelt, K. Hammer., and H.Knupffer (ed.). The genus Allium taxonomic problems andgenetic resources. Proceedings of an International Symposiumheld at Gatersleben, Germany, June 11-13, 1991. pp : 107-123