• The Korean Journal of Zoology
• /
• v.24 no.4
• /
• pp.201-215
• /
• 1981

In vitro intra- and interspecific molecular hybridizations of lactate dehydrogenase isozymes from six species were performed by freezing and thawing the isozymes in sodium chloride, resulting each of the interspecific hybridization of Fluta alba $H_4 \\times$ Ophicephalus argus $M_4$ and that of O. argus $M_4 \\times$ Parasilurus asotus isozymes showed one hybrid isozyme considered to be an $M_2H_2$ isozyme. The results fit with the suggestion that the isozyme of lower vertebrates is a dimer of homodimer as far as te assemblies of subunit M and subunit H are concerned.

• Journal of Life Science
• /
• v.10 no.2
• /
• pp.169-176
• /
• 2000

At Koppl area, undamaged natural habitat of sundew, I have analysed interspecific affinities and community composition through mathematical method and important environmental factors. Interspecific affinities of the species with frequency of more than 5% in vegetation table were analysed through chi-square test and showed obvious group of Drosera rotundifolia, Vaccinium uliginosum, Calluna vulgaris, Eriophorum angustifolium and 21 species. The result of ordination anlysis using DECORANA of VESPAN II showed eigenvalue of 0.6047 for axis I, 0.2024 for axis II and 0.0763 for axis Ⅲ. And it divided into 4 groups of quadrat number 1-5 for Sphagnum squarrosum-community, 6-10 for Crepis paludosa-community. 11-25 for Carex panicea-community and 26-35 for Scorpidium scorpioides-community. By the classification using TWINSPAN, the 7 areas divided into 2 groups of 1-10 and 11-35 at first level of division with high eigenvalue of 0.588 and indicator was Sphagnum squarrosum. At second level of division it divided into 4 groups as the results of DECORANA with eigenvalues of 0.268 and 0.423 and indicators were Pinguicula vulgaris and Scorpidium scorpioides. Microclimatic environment of studied area was low in temperature and high in humidity and soil environment showed high in field moisture capacity, acid, high organic matter content, low NO3 and K2O content, compare to normal soil, and high ground water level.

• Journal of Plant Biotechnology
• /
• v.29 no.3
• /
• pp.199-207
• /
• 2002

The tropane alkaloids hyoscyamine (its racemic form being atropine) and scopolamine are used medicinally as anticholinergic agents that act on the parasympathetic nerve system. Because they differ in their actions on the central nervous system, currently there is a 10-fold higher commercial demand for scopolamine, in the N-butylbromide form, than there is for hyoscyamine and atropine combined. Several solanaceous species have been used as the commercial sources of these alkaloids, but the scopolamine contents in these plants often are much lower than those of hyoscyamine. For this reason there has been long-standing interest in increasing the scopolamine contents of cultivated medicinal plants. Naturally occurring and artificial interspecific hybrids of Duboisia have high scopolamine contents and are cultivated as a commercial source of scopolamine in Australia and other countries. Anther culture combined with conventional interspecific hybridization also has been used to breed high scopolamine-containing plants in the genera Datura and Hyoscyamus, but without much success. The use of recombinant DNA technology for the manipulation of metabolic processes in cells promises to provide important contributions to basic science, agriculture, and medicine. In this review, I introduce on the enzymes and genes involved in tropane alkaloid biosynthesis and current progress in metabolic engineering approaches for tropane alkaloid, especially scopolamine, production.

• Horticultural Science & Technology
• /
• v.29 no.1
• /
• pp.74-76
• /
• 2011

An interspecific hybrid lily cultivar 'Green Star' was bred in 2005 at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The crossing and in vitro embryo rescue was conducted between Lilium FA97-2 (L. ${\times}$ formolongi 'Silky White' ${\times}$ L. Asiatic 'Sunray') and L. Asiatic 'Bomi (Byeongga ${\times}$ Connecticut King)' by cut style pollination method (CSM) at Suwon in 2000. The first selection was done and was tentatively named as 'FA03-5' in 2003. After in vitro multiplication and bulbing production of 'FA03-5' line, growth and flowering characteristic tests were conducted from 2003 to 2005. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS in 2005. 'Green Star' flowered in the middle of June and grew more than 120 cm stem in length. Flowers bloomed facing upward, unspotted in petals and greenish yellow (RHS, Y6D). 'Green Star' was male sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It was needed to control the Botrytis disease in wet season.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.26 no.6
• /
• pp.1237-1245
• /
• 1997

Interspecific fusants were made from the cells of two strains of Lactobacillus genus, a streptomycin resistant Lactobacillus sp. JC-7 and a kanamycin resistant L. acidophilus 88. The morphological and physiological properties of the fusants were examined by determining bacteriocin productivity, acid-producing activity, ability of carbohydrates utilization and three important enzyme activities. The fusants produced a bacteriocin against indicator strains and fusant No. 1, 4 exhibited a larger inhibition zone compared to that of L. acidophilus 88. $\beta$-Galactosidase, phospho-$\beta$-galactosidase, lipase activities and resistance to NaCl of Lactobacillus sp. JC-7 were better than those of L. acidophilus 88. Fusant No. 3 and 7 exhibited excellent lipase activities. Protease activity and acid productivity of L. acidophilus 88 were better than those of Lactobacillus sp. JC-7. Proteasse activities of all fusants were higher than those of parental strains, and expecially fusant No. 5 and 7 exhibited excellent proteolysis ability.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.52 no.4
• /
• pp.408-417
• /
• 2019

This study was conducted to identify taxonomic differences in the characteristics of Rhodeus uyekii and Rhodeus ocellatus during their initial life history via an interspecific hybridization experiment. Hybrids were compared to their parent species, and the findings were used to inform developmental research in commercially useful aquarium fish. The hatching rates of the cross-bred eggs were 75.9% for cross UO (R. uyekii ♀${\times}$R. ocellatus♂) and 71.9% for cross OU (R. ocellatus♀${\times}$R. uyekii♂), which did not differ greatly from the hatching rates of the normal cross-bred group. Backcross experiments resulted in 100% egg mortality during development. Newly hatched larvae of the original hybrid crosses were similar to those of the maternal line, and the color of the egg yolk was similar to that of the paternal line; therefore, the respective traits of the interspecific parents were identifiable within the cross-bred offspring.

• Animal Systematics, Evolution and Diversity
• /
• v.37 no.3
• /
• pp.235-241
• /
• 2021

To provide better taxonomic information of the genus Nectoneanthes, the two DNA barcode regions of mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA (rDNA) sequences of Nectoneanthes oxypoda and N. uchiwa were determined. In addition, the respective sequences of four nereidid species closely related to Nectoneanthes were retrieved from GenBank for comparison and to estimate intra- and inter-specific genetic distances. The aligned sequence lengths of COI and 16S rDNA were 570 bp and 419 bp long, respectively. The mean intraspecific variation in both markers was less than 1% in all species except for that in COI of H. diadroma (1.87%). The mean interspecific variation between N. oxypoda and N. uchiwa was 12.02% regarding COI and 1.85% regarding 16S rDNA. In contrast, the mean interspecific variation between species of other genera was comparably higher(i.e., genus Perinereis: 20.5% in COI and 8.3% in 16S rDNA; genus Hediste: 13.18% in COI and 2.64% in 16S rDNA), compared with that between the two Nectoneanthes species. This result indicated that these Nectoneanthes species are genetically more closely related than other congeneric species of different genera. The DNA barcoding information on Nectoneanthes species generated in this study provides valuable insights for further biodiversity studies on nereidid species.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.33 no.4
• /
• pp.392-399
• /
• 1988

The experiments were conducted to establish the effective barley haploid production system using interspecific crosses. Three spring barley cultivars. Bruce. Klages and Rodeo were used for this experiment. 1, 687 florets of three barley cultivars were crossed with bulbosum pollens. 1, 079 seeds were harvested and obtained 834 embryos so that seed set rate and embryo production rate were 64% and 77%, respectively. IAA effect was superior to NAA for root development and 1 ppm concentration of IAA gave the best result among five concentrations; 0ppm, 1ppm., 5ppm, 10ppm and 30ppm.

• Korean Journal of Plant Resources
• /
• v.31 no.1
• /
• pp.77-85
• /
• 2018

The cultivation area of rapeseed (Brassica napus L.) has been increased for oil production and landscaping purpose in Korea. However, as the color of rapeseed flower is very simple, diversified flower color is necessary to improve landscape effect. Interspecific and intergeneric crosses between rapeseed (Brassica napus) and three Cruciferae crops were performed in order to grow diverse flower color of rapeseed. The silique formation rate of interspecific cross rapeseed with cabbage (B. oleracea L) was relatively high (65.8%) and higher than intergeneric cross with rapeseed and radish (Raphanus sativus L.), rapeseed and Orychophragmus, respectively. During silique developing period after artificial pollination, there were many siliques without seeds due to the failure of fertilization. The average number of seed per silique obtained from cross rapeseed and cabbage, rapeseed and radish, rapeseed and O. violaceus were 0.12, 0.4 and 0.12, respectively. The phenotypes of $F_1$ hybrid plants from cross rapeseed and Cruciferae crops were mostly similar to maternal line, but leaf length and leaf width were increased. The interspecific cross of rapeseed and cabbage generated ivory color of flower which is the medium color of parents, and intergeneric cross of rapeseed and O. violaceus created entities with larger flowers which seems to enhance landscape effect. The fatty acid composition of most hybrid seeds intermediated of the two parents for oleic acid, linoleic acid and linolenic acid, content. Whereas hybrid of rapeseed and radish produced less erucic acid than radish parent.

• Journal of Korean Society of Forest Science
• /
• v.98 no.4
• /
• pp.379-391
• /
• 2009

This study was carried out to classify forest vegetation structure of Mt. Munsu and Mt. Okseok from May to September in 2008 using phytosociological analysis methodology of Z-M school under the purpose of furnishing basic materials for rational and scientific forest management. Total of one hundred five study sites (quadrats) was investigated in the area. Quercus mongolica community group was divided into 5 communities such as Pinus densiflora for. erecta community, Hydrangea serrata for. acuminata community, Salix gracilistyla community, Pinus koraiensis community and Larix kaempferi community. Pinus densiflora for. erecta community was subdivided into Rhododendron micranthum group, Quercus variabilis group and Pinus densiflora for. erecta typical group. Hydrangea serrata for. acuminata community also into Ligularia fischeri group, Deutzia glabrata group (into Juglans mandshurica subgroup, Prunus maackii subgroup and Callicarpa japonica subgroup). Accordingly, the vegetation of the study areas could be described as 1 community group, 5 communities, 5 groups, and 3 subgroups and as 10 units in total. Classified vegetation units were highly correlated with altitude and topography. According the analysis of interspecific association, it was shown as three types which were coincident with differential species and character species on the constancy table.