• Title/Summary/Keyword: International Agricultural Research Institute

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Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

  • Zhang, Zhi-Fang;Shen, Xing-Jia;Yi, Yong-Zhu;Tang, Shun-Ming;Li, Yi-Ren;He, Jia-Lu;Wu, Xiang-Fu
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.169-174
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    • 2004
  • Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (lue) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 - 2.0 $\mu\textrm{g}$/$m\ell$ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5-2.0 ${\mu}g$/$m\ell$ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

Global Trade Networks of Agro-Food and the Status and Prospects in Korea (농식품 무역의 글로벌 네트워크와 한국의 위상)

  • Hyun, Kisoon;Lee, Junyeop
    • Journal of the Economic Geographical Society of Korea
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    • v.20 no.2
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    • pp.121-136
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    • 2017
  • In this paper, using trade data of 57 HS 4 digit based agricultural and food(agro-food) products among 47 countries during 2005 to 2014, the international competitiveness and trade structure have been analyzed from the context of global networks employing the methods of social network analysis. Firstly, the differences in the network structure by agricultural products have been revealed. The number of disconnected groups was significantly lager in order of vegetables, fruits and processed foods. Secondly, the differences in the community structure by agricultural and food products have been also revealed. That is to say, for some commodities, the community structure has been changed dynamically, on the other hand, there are some agricultural products that have not changed its community structure despite the increasing trends of trade volume. Thirdly, even though the international competitiveness of Korea's agricultural products was still very limited in the sense that only 26 items have been included in the top3 network of 57 agricultural products, there has been possibilities of the increasing patterns of the competitiveness.

Orientia tsutsugamushi Infection Induces $CD4^+$ T Cell Activation via Human Dendritic Cell Activity

  • Chu, Hyuk;Park, Sung-Moo;Cheon, In Su;Park, Mi-Yeoun;Shim, Byoung-Shik;Gil, Byoung-Cheol;Jeung, Woon Hee;Hwang, Kyu-Jam;Song, Ki-Duk;Hong, Kee-Jong;Song, Manki;Jeong, Hang-Jin;Han, Seung Hyun;Yun, Cheol-Heui
    • Journal of Microbiology and Biotechnology
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    • v.23 no.8
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    • pp.1159-1166
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    • 2013
  • Orientia tsutsugamushi, a gram-negative bacterium, causes severe acute febrile illness in humans. Despite this danger, the route of infection, infectivity, and protective mechanisms of the host's immune response to O. tsutsugamushi are unclear. Dendritic cells (DCs) are one of the most important cell types in bridging the innate and adaptive immune responses. In this study, we observed that O. tsutsugamushi infects and replicates in monocyte-derived DCs (MODCs). During infection and replication, the expressions of the cytokines IL-12 and TNF-${\alpha}$, as well as the co-stimulatory molecules CD80, CD83, CD86, and CD40, were increased in MODCs. When O. tsutsugamushi-treated MODCs were co-cultured with autologous $CD4^+$ T cells, they enhanced production of IFN-${\gamma}$, a major Th1 cytokine. Collectively, our results show that O. tsutsugamushi can replicate in MODCs and can simultaneously induce MODC maturation and increase proinflammatory cytokine levels in MODCs that subsequently activate $CD4^+$ T cells.

Studies on the Seasonal Incidence of the Whitefly (Dialeuropora decempuncta Quaintance and Baker) Causing Leaf Curl on Mulberry in Relation to Abiotic Factors

  • Bandyopadhyay, U.K.;Sahu, P.K.;Raina, S.K.;Santhakumar, M.V.;Chakraborty, N.;Sen, S.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.1 no.1
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    • pp.65-71
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    • 2000
  • A study was conducted to examine the relationship between abiotic factors and the population of white-fly (Dialeuropora decempuncta) in mulberry field. The study reveals that relationship between abiotic factors and the whitefly population is very much existent like other pests in other agricultural crops. Duration and time of distinct occurrence of whitefly in mulberry is influenced by the abiotic conditions of field. Abiotic parameters of previous month are more important in influencing the intensity of the pest than the current abiotic factors. Not all the abiotic factors are equally important but factors like minimum temperature, fluctuation in temperature during the days minimum relative humidity, fluctuation in relative humidity and rainfall are the major important lactors in influencing the intensity of the pest under consideration.

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Dynamic Masterplan of the Saemangeum Grain Complex for Progressive Development (점진적 개발 단계를 고려한 새만금 복합곡물단지의 동태적 마스터플랜 수립)

  • Jung, Chanhoon;Kim, Chanwoo;Kim, Solhee;Park, Jinseon;Seo, Donguk;Suh, Kyo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.60 no.4
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    • pp.1-13
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    • 2018
  • The grain complex of Saemangeum is created for promoting the foundation of agriculture combined the global competitiveness. However, the masterplan is being also revised with changing of local conditions and social needs. Thus, the dynamic masterplan is needed to consider the change of time for Saemangeum project. The present study was made to set up the dynamic masterplan of Saemangeum grain complex for handling the change such as project progress, local environment, and project conditions flexibly. In this study, the dynamic masterplan for the progressive development of water supply, farmland composition, and introduction facilities is presented to the 6-2 zone in three stages. We believed that the water supply would be possible through the pumping and desalination facilities with the development stages. The farmland composition proceeded for each complex with reclamation, soil preparation, and soft soil processing. And it is planned to carry out crop cultivation from the complex where the construction is completed first. The introduction facilities were analyzed focusing on the silos and forage loading facilities, and the optimal location of them was selected using road and accessibility. The concept of dynamic masterplan may provide the direction for the planning and progress of reclamation project.

Physicochemical characteristics and antioxidant potential of paprika (Capsicum annuum L.) wine

  • Kim, Chan Yong;Kwon, Oh Hun;Gun, Won Jong;Park, Yong-Sung;Dhungana, Sanjeev Kumar;Kim, Il-Doo
    • Korean Journal of Food Science and Technology
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    • v.51 no.6
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    • pp.592-595
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    • 2019
  • Paprika (Capsicum annuum L.) contains various phytochemicals, including carotenoids, ascorbic acid, tocopherol, flavonoids, and phenolic compounds, as well as natural food colorants. Very little information is available regarding wine produced from different colored paprikas. The objectives of this study were to prepare wines from red, orange, and yellow paprika and evaluate their physicochemical characteristics. The alcohol concentration, pH, titratable acidity, and reducing sugar content were not significantly affected by the type of paprika. Hunter's color values varied with the color of paprika. The total mineral content and 2,2-diphenyl-1-picrylhydrazyl radical scavenging potential of red paprika wine were significantly higher; however, the total polyphenol content of yellow paprika wine was significantly higher than that of the other wine samples. This study suggested that paprika could be used to prepare wine and red paprika might be appropriate for producing good-quality wine.

Hari Intensive Field Experiment (HIFE): Overview (하리 집중 야외 실험: 개요)

  • Kim, J.;Yun, J.I.;Ryoo, S.B.;Kim, J.K.;Yun, S.H.;Lim, J.H.;Lee, D.Y.;Kim, M.Y.;Kim, K.H.;Hong, K.H.
    • Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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    • 2001.06a
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    • pp.1-2
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    • 2001
  • 1. 연구목적 : 현재 한반도 내에서 관측을 수행하고 있거나 계획중인 지표 플럭스 관련 연구자들간의 관측 시스템의 상호 비교, 경계층 동시관측, 이산화탄소를 포함한 다양한 미량기체 플럭스 관측 및 지면과정 모형의 운용과 검정을 위한 실측 자료 확보, 분석 및 활용 2. 논의 : 하리 HIFE 집중실험에 참여한 연구팀들의 관측 시스템 및 연구 목적을 간략히 소개하고 이들간의 국내 공동 연구 및 네트워크 구축의 필요성에 대하여 소개한다.(중략)

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Development of Agricultural Water Circulation Rate Considering Agricultural Reservoir and Irrigation District (농업용 저수지 및 관개지구를 고려한 농업유역 물순환율 개발)

  • Kim, Seokhyeon;Song, Jung-Hun;Hwang, Soonho;Kim, Hak-Gwan;Kang, Moon Seong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.62 no.2
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    • pp.83-95
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    • 2020
  • The water circulation in agricultural watersheds changes with the operation of agricultural reservoirs, it is necessary to classify and evaluate them into upstream, agricultural reservoirs, irrigation districts, and downstream. Therefore, in this study, we developed the agricultural water circulation rate (AWCR) considering an agricultural reservoir and irrigation district by improving the water circulation rate of the Water environmental conservation Act. we applied it to Jinwi watershed using the module-based hydrologic analysis system to simulate the water circulation for agricultural reservoirs and irrigation areas. The model performance during the validation period was NSE of 0.762 for the downstream stream and 0.682 for the reservoir level. And the hydrograph separation model was applied to separate the direct and baseflow. As a result of this study, The AWCR of Jinwi watershed was 71.8% on average, which was higher than the water circulation rate estimated by the downstream hydrograph separation.

A Study on Japanese Export Promotion Strategy for agricultural products and our countermeasures (일본의 농산물 수출촉진 전략과 우리의 대응)

  • Lee, Won-Keun
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.47
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    • pp.357-377
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    • 2010
  • The Ministry of Agriculture, Forestry and Fisheries(MAFF) of Japan established the aim which exports the agriculture, forestry and Fisheries products amounting to a trillion Yen until 2013 in 2004, endeavoring to realize the aim in strengthening both overseas publicity activities and domestic product system. Such changes means that the Japanese agriculture is exchanging defence into attack and gives a important meaning to our agricultures which have a similar agriculture base as Japan. The countermeasures for agricultural products export promotion by MAFF is greeting a 5th year now and obtains a considerable result in agricultural products exports and the export item. Meantime, There are also opinions that the Japanese agriculture has to make efforts to cut the product cost by a large margin in order to prepares a competitive power. we have to consider the counter-measure which will be able to accommodate harmoniously both of two facts that first, Japanese Export Promotion Strategy for agricultural products is giving a hope in the Japanese rural society which have been considered that remaking will be impossible until now, and secondly, the agricultural reform which the cutting of cost and price of agricultural products will be possible in has to be promoted.

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