• Title/Summary/Keyword: International Agricultural Research Institute

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Enhanced Biosynthesis of ${\alpha}$-tocopherol in Transgenic Soybean by Introducing ${\gamma}$-TMT gene

  • Kim Young-Jin;Seo Hong-Yul;Park Tae-Il;Baek So-Hyeon;Shin Woon-Chul;Kim Hyun-Soon;Kim Jung-Gon;Choi Yong-Eui;Yun Song-Joong
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.203-209
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    • 2005
  • This study was conducted to improve tocopherol (vitamin E) composition in soybean (Glycine max) by introducing a gamma-tocopherol methyl transferase (${\gamma}$-TMT) gene via Agrobacterium tumefaciens-mediated transformation. Immature cotyledon explants were cocultivated with Agrobacterium tumefaciens. Putative transgenic embryos were selected from immature cotyledons on MS medium supplemented with 40 mg/L 2,4-D containing 100 mg/L kanamycin, 500 mg/L carbenicillin and 250 mg/L cefotaxime. Plantlets were developed from somatic embryos, and then transferred to soil. Nineteen regenerated plantlets obtained on the selection medium from 1,460 cotyledons. However, only 9 plantlets were confirmed as transformed plants. Integration of the transgene into the soybean genomic DNA was confirmed by PCR and Southern blot analysis. HPLC analysis showed that the content of ${\alpha}$-tocopherol in transgenic soybean seeds (AT-1) was approximately 4-fold higher than that of non-transgenic plants. Conclusively, we obtained the transgenic soybean having increased ${\alpha}$-tocopherol content by the overexpression of ${\gamma}$-TMT transgene.

Development of Evaluation Indicators for Web-based Agricultural Water Information System using Mandal-Art Method (만다라트(Mandal-Art) 기법을 이용한 웹기반 농업용수 정보시스템 평가지표 개발)

  • Kim, Solhee;Kim, Chanwoo;Jung, Chanhoon;Park, Jinseon;Kim, Jeongdae;Suh, Kyo
    • Journal of Korean Society of Rural Planning
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    • v.23 no.4
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    • pp.49-59
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    • 2017
  • The importance of information system evaluation has increased according to ICT development and this evaluation is required for objective verification. The aim of this study is to develop evaluation indicators for web-based information system and verify the availability of these evaluation indicators through applying to agricultural water integrated information system. This study finds and provides eight evaluation items and 64 indicators for a web-based information system using $3{\times}3$ Mandal-Art matrix that is a tool for creating creative ideas. These evaluation items are design, contents, navigation, stability, community, convenience, usefulness, and accuracy. Total 64 evaluation indicators were presented by deriving eight evaluation items for each using $9{\times}9$ Mandal-Art matrix. When evaluating information system using evaluation indicators, it can be identified the vulnerable items in the information system. Also, the comprehensive results of the information system could be understood when appearing a single score after weighting. In addition, it can also help to prepare a questionnaire for evaluation systematically.

Breeding ova New Silkworm Variety, Chunsujam, with a High Silk Yielding for Spring Rearing Season

  • Kang, Pil-Don;Kim, Kye-Myeong;Sohn, Bong-Hee;Lee, Sang-Uk;Woo, Soon-Ok;Hong, Sung-Jin
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.1
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    • pp.65-68
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    • 2001
  • A new silkworm variety, Chunsujam, for spring rearing season is F$_1$ hybrid between Japanese race Jam145 bred by 8459/8711 and Chinese race Jam146 by 8544/M8626. Jam145, Japanese parent of the Chunsujam, showed high GCA in pupation rate and Jam146, Chinese parent, showed high GCA in pupation rate and single cocoon weight. In the local adaptability test performed at 8 local areas in spring of 1999, Chunsujam was 5% higher in larval weight, 3% in single cocoon weight, and 4% in cocoon yield from 10,000 of the 3rd molted larvae, respectively, than the check variety Baegokjam.

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Utilization of snails as food and therapeutic agents by Baiga tribals of Baihar tehsil of Balaghat District, Madhya Pradesh, India

  • Baghele, Mahendra;Mishra, Shubhi;Meyer-Rochow, Victor Benno;Jung, Chuleui;Ghosh, Sampat
    • International Journal of Industrial Entomology and Biomaterials
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    • v.43 no.2
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    • pp.78-84
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    • 2021
  • We explored the indigenous local knowledge associated with the use of snails by Baiga people in Baihar tehsil of Balaghat district in central India through interview with a semi-structured questionnaire. Results revealed that Baiga people widely accept snails of 3-5 morpho-groups belonging to Ampullariidae, and Viviparidae family as food. Besides, use of snail as therapeutic agents for ailments like body ache, joint pain, digestion, weakness of bones etc. were found common. Hand-picking of snails from the wild environments as well as agriculture field was the primary way of harvesting edible snails. We advocate the development of a farming system for the continuous supply of snails as a food resource, advancement of scientific investigation of functional properties of them, economic progress of the region and therefore the overall sustainability.

Present and Future Agricultural Extension System and International Cooperation Systems for Agricultural Technologies in Ghana (가나의 농촌지도사업과 국제협력사업의 현재와 미래)

  • Isaac, Ansah;Cho, Gyoung-Rae
    • Journal of Agricultural Extension & Community Development
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    • v.21 no.2
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    • pp.143-184
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    • 2014
  • The purpose of this study is to explore the present and future agricultural extension system and international cooperation systems for agricultural technologies in Ghana. The role of agricultural extension in the next decade should be quite different from what it was 10 years ago or even now. Its role as a facilitator of agricultural knowledge system would only increase as more participants from private sector would get involved in extension. The public sector extension would still continue to be the major extension provider in most parts of the country as the private sector alone would not be able to meet even partially the varied needs of farmers. Internal reforms are thus going to be the greatest challenge for the Ghana Extension System. The cooperation systems work in partnership with other stakeholder to provide agricultural extension and advisory services to farmers. Innovations (information/knowledge) emerging from the seed industry may be seen as dynamic and systemic process which can emerge from many sources. KAFECI's interventions must be flexible to accommodate such changes if the need arises in subsequent years (year 2 & 3). The framework and tools for technology transfer and dissemination is developed on "innovation platform" where a group of stakeholders/collaborators with diverse social and economic backgrounds, work towards a common objective or challenge.

Investigation of mulberry farm's soil properties and mulberry leaf nutritive components in local areas of Korea

  • Ju, Wan-Taek;Jeong, Chan Young;Kim, Seong-Wan;Park, Jong Woo;Kim, Nam-Suk;Kang, Sang Kuk;Kim, Kee-Young;Kweon, Hae-Yong;Lee, Seul-Bi;Kim, Woong;Gwak, Byeong-Sam;Han, Bong-Tae;Choi, Moon-Tae;Lee, Yoo Beom;Seok, Young-Seek
    • International Journal of Industrial Entomology and Biomaterials
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    • v.45 no.1
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    • pp.35-41
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    • 2022
  • Mulberry is a hardy, perennial, deep-rooted plant capable of thriving under diverse agroclimatic conditions. The selection of suitable land and appropriate variety can help the sustainable mulberry field. However, no conclusive and comprehensive investigation has been conducted on the mulberry soil properties and nutritional composition of mulberry cultivars from Korea local areas in previous studies. In our study, soil properties and mulberry leaf components of Korea local mulberry farms were briefly investigated. In result, the soil organic matter (OM) content was significantly high in Buan (6.81%) and Jangseong (6.14%). In contrast, available phosphate (P2O5) was different in each local area. To investigate relationship between soil property and nutritive component of mulberry leaf, Cheongil leaf samples from 8 local areas were analyzed. Among the macrominerals (K+, Ca2+, Na+, and Mg2+), the concentration of K varied from (1884±9.36) mg/100 g to (2685±11.5) mg/100 g. The potassium (K+) of Cheongil leaf in Sangju was the highest at 2685 mg/100 g. Besides macrominerals, flavonoids, total dietary fiber contents and moisture of Cheongil leaf samples were studied in the 8 local areas. In terms of these contents, the variation was largely depending on the local areas. This study provides a possible industrial use of mulberry, and holds promise to enhance the overall profitability of sericulture.

Comparison of Forage Quality, Productivity and β-carotene Content according to Maturity of Forage Rye (Secale cereale L.)

  • Zhao, Guo Qiang;Wei, Sheng Nan;Li, Yan Fen;Jeong, Eun Chan;Kim, Hak Jin;Kim, Jong Geun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.3
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    • pp.123-130
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    • 2020
  • These experiments were to investigate the variations of rye on forage quality, productivity and β-carotene concentration affected by maturity in Pyeongchang region. Limited information are available about how forage quality and β-carotene content are affected by various factors. Samples were collected from rye harvested every 5 days, from April 25 to May 31 (April 25, April 30, May 4, May 9, May 15, May 21, May 25 and May 31). Dry matter (DM) content, plant height, DM yield and total digestible nutrient (TDN) yield increased continuously with the progressed maturity. However, crude protein (CP) content, in vitro dry matter digestibility (IVDMD) and relative feed value (RFV) decreased markedly with the delay of harvesting, while TDN content decreased from April 25 till May 15, then followed by a stable fluctuation. Conversely, acid detergent fiber (ADF) and neutral detergent fiber (NDF) value increased and then fluctuated slightly after blooming stage. For quality of plant parts, stem contained the lowest CP content and RFV value, and the highest ADF and NDF contents compared with other parts, while the grain showed the higher CP, IVDMD, RFV and lower fiber contents than others. With the plant matured, leaf proportion decreased while stem and grain proportion increased, and feed value of all the three parts decreased till blooming stage and followed by a stable phase. β-carotene concentration showed its highest on jointing stage, and then fell down sharply on the sequential stages. In conclusion, harvest around May 15 (blooming) is proper for forage rye if directly consumed by livestock as green chop in Pyeongchang under the consideration of both nutritive yield and forage quality.

Calculating virtual water for international water transactions : Korea focused international trade analysis (국제 물거래 대비 가상수 거래량 산정 : 한국 중심 국제교역량 분석)

  • Park, Sungje;Lee, Minhyeon;Park, Kyeyoung;Shin, Jihye
    • Journal of Korea Water Resources Association
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    • v.53 no.9
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    • pp.691-699
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    • 2020
  • South Korea's available water resources per capita is very small compared to global average as there is a high population on small land. Thus, it is imperative to secure water resources for the safe livelihood of the citizens. As an advanced, industrialized country, South Korean economic dependence on agricultural has greatly lowered. Unless South Korea utilizes foreign water resources, it is impossible to provide agricultural and livestock products the same as current levels. However, virtual water-related research in South Korea is still inadequate. When establishing the water resources plan, it does not consider the international trade of virtual water. This research aims to solve this issue by analyzing the international virtual water trends focusing on South Korea. Consequently, the export and import of virtual water trade was identified according to country and item for 248 countries and South Korea. According to the results, South Korea's agricultural and livestock virtual water has much higher imports than exports, which outputs with agricultural by-products being the main import. In 2018, South Korea imported 72.2 billion ㎥, which is 29 times the exported amount of 2.5 billion ㎥. The research results can be used as baseline data for establishing the national water resources plan in the future.

Evaluation of forage production, feed value, and ensilability of proso millet (Panicum miliaceum L.)

  • Wei, Sheng Nan;Jeong, Eun Chan;Li, Yan Fen;Kim, Hak Jin;Ahmadi, Farhad;Kim, Jong Geun
    • Journal of Animal Science and Technology
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    • v.64 no.1
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    • pp.38-51
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    • 2022
  • Whole-plant corn (Zea may L.) and sorghum-sudangrass hybrid [Sorghum bicolor (L.) Moench] are major summer crops that can be fed as direct-cut or silage. Proso millet is a short-season growing crop with distinct agronomic characteristics that can be productive in marginal lands. However, information is limited about the potential production, feed value, and ensilability of proso millet forage. We evaluated proso millet as a silage crop in comparison with conventional silage crops. Proso millet was sown on June 8 and harvested on September 5 at soft-dough stage. Corn and sorghum-sudangrass hybrid were planted on May 10 and harvested on September 10 at the half milk-line and soft-dough stages, respectively. The fermentation was evaluated at 1, 2, 3, 5, 10, 15, 20, 30, and 45 days after ensiling. Although forage yield of proso millet was lower than corn and sorghum-sudangrass hybrid, its relative feed value was greater than sorghum-sudangrass hybrid. Concentrations of dry matter (DM), crude protein, and water-soluble carbohydrate decreased commonly in the ensiling forage crops. The DM loss was greater in proso millet than those in corn and sorghum-sudangrass hybrid. The in vitro dry matter digestibility declined in the forage crops as fermentation progressed. In the early stages of fermentation, pH dropped rapidly, which was stabilized in the later stages. Compared to corn and sorghum-sudangrass hybrid, the concentration of ammonia-nitrogen was greater in proso millet. The count of lactic acid bacteria reached the maximum level on day 10, with the values of 6.96, 7.77, and 6.95 Log10 CFU/g fresh weight for proso millet, corn, and sorghum-sudangrass hybrid, respectively. As ensiling progressed, the concentrations of lactic acid and acetic acid of the three crops increased and lactic acid proportion became higher in the order of sorghum-sudangrass hybrid, corn, and proso millet. Overall, the shorter, fast-growing proso millet comparing with corn and sorghum-sudangrass hybrid makes this forage crop an alternative option, particularly in areas where agricultural inputs are limited. However, additional research is needed to evaluate the efficacy of viable strategies such as chemical additives or microbial inoculants to minimize ammonia-nitrogen formation and DM loss during ensiling.

Correlation Analysis between Meteorological Factors and Crop Products (농산물 생산량과 기상요소의 상관관계 분석)

  • Lee, Ki-Kwang;Ko, Kwang-Kun;Lee, Joong-Woo
    • Journal of Environmental Science International
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    • v.21 no.4
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    • pp.461-470
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    • 2012
  • Agriculture is more influenced by environmental factors rather than other industries. Among the environmental factors, the meteorological conditions mainly impact the output of agricultural products. Hence, the purpose of this study is to analyze the impact of meteorological factors on the output of elemental agricultural products. As a first step, we obtained the data of the meteorological factors (i.e., precipitation, humidity, temperature, insolation, snowdrifts, wind velocity) and the output of the various agricultural products (i.e., grain, fruits and vegetables, root crops, green vegetables, seasoned vegetables, fruits, special crops) from the year 1990 to 2009 (20 years) of Seoul and the six metropolitan cities in Korea. Then, the analysis of the correlation between the agricultural product with the largest output and the meteorological factors of the place where the corresponding agricultural product is most produced, was carried out in order to determine the core meteorological factor that most impacts the output of agricultural product. The correlation analysis revealed that humidity, insolation and wind velocity have been the crucial meteorological factors to influence the output of the agricultural products. From the result, we can induce that the meteorological forecast information about the vital meteorological factors, i.e., humidity, insolation and wind velocity, facilitates the optimized cultivation plan to maximize the output of agricultural products.