Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Enhanced Biosynthesis of ${\alpha}$-tocopherol in Transgenic Soybean by Introducing ${\gamma}$-TMT gene

  • Kim Young-Jin (Honam Agricultural Research Institute, NICS, RDA) ;
  • Seo Hong-Yul (Honam Agricultural Research Institute, NICS, RDA) ;
  • Park Tae-Il (Honam Agricultural Research Institute, NICS, RDA) ;
  • Baek So-Hyeon (Honam Agricultural Research Institute, NICS, RDA) ;
  • Shin Woon-Chul (Honam Agricultural Research Institute, NICS, RDA) ;
  • Kim Hyun-Soon (Multilateral Cooperation Team, International Technical Cooperation Center, RDA) ;
  • Kim Jung-Gon (Honam Agricultural Research Institute, NICS, RDA) ;
  • Choi Yong-Eui (Division of Forest Resources, College of Forest Sciences, Kangwon National University) ;
  • Yun Song-Joong (Faculty of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University)
  • Published : 2005.09.01
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Abstract

This study was conducted to improve tocopherol (vitamin E) composition in soybean (Glycine max) by introducing a gamma-tocopherol methyl transferase (${\gamma}$-TMT) gene via Agrobacterium tumefaciens-mediated transformation. Immature cotyledon explants were cocultivated with Agrobacterium tumefaciens. Putative transgenic embryos were selected from immature cotyledons on MS medium supplemented with 40 mg/L 2,4-D containing 100 mg/L kanamycin, 500 mg/L carbenicillin and 250 mg/L cefotaxime. Plantlets were developed from somatic embryos, and then transferred to soil. Nineteen regenerated plantlets obtained on the selection medium from 1,460 cotyledons. However, only 9 plantlets were confirmed as transformed plants. Integration of the transgene into the soybean genomic DNA was confirmed by PCR and Southern blot analysis. HPLC analysis showed that the content of ${\alpha}$-tocopherol in transgenic soybean seeds (AT-1) was approximately 4-fold higher than that of non-transgenic plants. Conclusively, we obtained the transgenic soybean having increased ${\alpha}$-tocopherol content by the overexpression of ${\gamma}$-TMT transgene.

Keywords

References

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