• 제목/요약/키워드: International Agricultural Research Institute

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Anthocyanin and proanthocyanidin contents, antioxidant activity, and in situ degradability of black and red rice grains

  • Hosoda, Kenji;Sasahara, Hideki;Matsushita, Kei;Tamura, Yasuaki;Miyaji, Makoto;Matsuyama, Hiroki
    • Asian-Australasian Journal of Animal Sciences
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    • 제31권8호
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    • pp.1213-1220
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    • 2018
  • Objective: An experiment was conducted to assess the antioxidant contents and activities of colored rice grains and to evaluate their nutritive characteristics in terms of chemical composition and in situ ruminal degradation. Methods: Ten cultivars of colored rice grains (Oryza sativa L.) collected from several areas of Japan were studied, and control rice without pigment, maize, barley, and wheat grains were used as control grains. Their chemical compositions, pigment, polyphenol contents, total antioxidant capacity (TAC), and degradation characteristics were determined. Results: The starch contents of the colored rice grains were in the range of 73.5% to 79.6%, similar to that of the control rice grain. The black and red rice grains contained anthocyanin (maximum: $5,045.6{\mu}g/g$) and proanthocyanidin (maximum: $3,060.6{\mu}g/g$) at high concentrations as their principal pigments, respectively. There were significantly (p<0.05) positive relationships among the pigment contents, polyphenol content, and TAC values in the colored and control rice grains, indicating that the increase in pigment contents also contributed to the increased polyphenol content and TAC values in the colored rice grains. The dry matter and starch degradation characteristics, as represented by c (fractional degradation rate of slowly degradable fraction) and by the effective degradability, of the colored rice grains and the control rice grain were ranked as follows among commonly used grains: wheat>barley${\geq}rice$>maize. The colored rice grains also included the most-digestible starch, since their potential degradable fraction and actual degradability at 48 h incubation were almost 100%. Conclusion: Colored rice grains have high potential to be used as antioxidant sources in addition to starch sources in ruminants.

Smallholder Pig Rearing Systems in Northern Lao PDR

  • Phengsavanh, P.;Ogle, B.;Stur, W.;Frankow-Lindberg, B.E.;Lindberg, J.E.
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권6호
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    • pp.867-874
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    • 2011
  • This paper describes the results of a survey aimed at characterizing smallholder pig production systems in northern Lao PDR. A total of 341 households from five Northern provinces were interviewed in the survey. Village meetings and individual interviews, using a semi-structured questionnaire, were used to collect information. Three main pig rearing systems, free-scavenging, semi-scavenging and confinement (enclosures and pens), were found in the survey areas. These systems were practiced differently by smallholders depending on the level of intensity of crop production, ethnicity and purpose of keeping pigs. The confinement system was mainly practiced by Lao-Tai and Tibeto-Burman groups, who mainly bought piglets and fattened these pigs for sale. In contrast, the Mon-Khmer and Hmong-Mien reared pigs in free-scavenging and semi-scavenging systems, and usually keep sows for piglet production. The main factors that affected the changes in rearing systems were found to be level of intensity of crop production, local regulations and outbreaks of disease. The main constraints found in smallholder systems were outbreaks of disease, high mortality of piglets and the slow growth rate of fattening pigs.

Food plants suitable for mass rearing of the coconut hispine beetle Brontispa longissima

  • Yamashita, Ai;Winotai, Amporn;Nakamura, Satoshi;Takasu, Keiji
    • Korean Journal of Agricultural Science
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    • 제36권1호
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    • pp.57-61
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    • 2009
  • The invasive pest Brontispa longissima(Coleoptera: Chrysomelidae), native to Indonesia and Papua New Guinea, has extended its distribution to Australia, Asia and Pacific islands and caused serious leaf damages of the coconut palm Cocos nuciferain the invaded regions. Although biological control using parasitic wasps has successfully reduced population density and leaf damage levels, this pest and its natural enemies have not been efficiently producedin conventional methods using young leaves of C. nucifera. In the present study, we examined suitability of plants easily available in Thailand and Japan for mass rearing of this pest to develop effective mass rearing system of this pest. Mature, green leaves of the palms were also suitable for immature development and adult reproduction of this pest. Since mature leaves of C. nucifera are more abundant and less contaminated with fungus than the unopened leaf buds, mature leaves could be a promising plant diet for mass rearing of B. longissima. Ornamental palms such as Hyophorbe lagenicaulis and Washingtonia filifera were also suitable for immature development and reproduction of B. longissima. Away from palms, the cattail Typha spp. can sustain immature development and adult reproduction of B. longissima. In the area where C. nucifera is rare or not available, W. filifera or Typha spp. would be good food plants for mass rearing of this pest.

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Complete Genome Sequence of the RNAs 3 and 4 Segments of Rice stripe virus Isolates in Korea and their Phylogenetic Relationships with Japan and China Isolates

  • Jonson, Miranda Gilda;Choi, Hong-Soo;Kim, Jeong-Soo;Choi, Il-Ryong;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • 제25권2호
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    • pp.142-150
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    • 2009
  • The complete genome sequences of RNA3 and RNA4 of the 13 different Rice stripe virus (RSV) isolates were determined and characterized in this study to address the possible causes of the recent re-emergence of RSV that affected many rice fields in Korea. The genome size of each RNA segment varied among isolates and significant differences were observed in the intergenic region. There was up to 4% average divergence in the RNA4 nucleotide sequence among 13 Korean isolates and only 1.4% in the RNA3. Phylogenetic relationships among different Korean isolates revealed that there were at least 2 types of RNA3 and 4 distinct types of RNA4 genomes present in Korea. However, Korean isolates with one type of RNA3 predominate over the other while the occurrences of the RSV Korean isolates with the 4 types of RNA4 genome were not correlated to specific geographical areas. Results further indicate that RNA4 had diverged more than RNA3 and these differences in accumulation of mutations in the individual RNA segments indicate that genetic reassortment were likely to contribute to the genetic divergence in the 13 Korean isolates. All of the Korean-RNA3 sequences except for one isolate grouped with Chinese isolates (JY and Z). In contrast, the RNA 4 sequences segregated together with either Chinese (JY and Z) and Japanese (M and T) isolates but genetic relationships of Korean isolates- RNAs 3 and 4 segments to Chinese-Y isolate were low. Altogether, these results suggest that the occurrence of mixtures of RNAs 3 and 4 genotypes in the natural population of RSV may have contributed to the sudden outbreak in Korea.

Evaluation of Phytochemical econtents and antioxidant activity of Korean common bean (Phaseolus vulgaris L) landraces (한국 재래종 강낭콩 유전자원의 phytochemical 및 항산화 활성 평가)

  • Lee, Kyung Jun;Shin, Myoung-Jae;Cho, Gyu-Taek;Lee, Gi-An;Ma, Kyung-Ho;Chung, Jong-Wook;Lee, Jung-Ro
    • Journal of the Korean Society of International Agriculture
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    • 제30권4호
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    • pp.357-369
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    • 2018
  • The Korean common bean (Phaseolus vulgaris L.) has been receiving increased attention as a functional food. The objective of this study was to reveal the phytochemicals genetic variation and antioxidant activity of 209 Korean common bean landraces. Antioxidant activity was evaluated with the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power (FRAP), and superoxide dismutase (SOD) assay. Antioxidant activities among common bean accessions showed wide variation. Four flavonoids (kaempferol, myricetin, quercetin, and naringenin) of the 209 Korean common bean landraces were measured using HPLC. Among them, kaempferol had the highest phytochemicals compared to the other three flavonoids. Using the relative antioxidant capacity index (RACI), it was found out that the IT104587 had the highest antioxidant activity. Meanwhile, in clustering analysis, the Korean common bean landraces were classified into three clusters. Among them, cluster II contained 64 landraces with higher antioxidant activities and phytochemicals than the other clusters, except DPPH. The results could provide information on the valuable Korean common bean landraces for the development of new common bean varieties.

Effects of feeding different varieties of faba bean (Vicia faba L.) straws with concentrate supplement on feed intake, digestibility, body weight gain and carcass characteristics of Arsi-Bale sheep

  • Wegi, Teklu;Tolera, Adugna;Wamatu, Jane;Animut, Getachew;Rischkowsky, Barbara
    • Asian-Australasian Journal of Animal Sciences
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    • 제31권8호
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    • pp.1221-1229
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    • 2018
  • Objective: A study was conducted to evaluate the varietal differences among faba bean straws and also to assess the potentials of faba bean straws supplemented with concentrate fed at the rate 70% straws and 30% concentrate mixture on feed intake, digestibility, body weight gain and carcass characteristics of the animals. Methods: Forty yearling Arsi-Bale sheep with initial body weight of $19.85{\pm}0.29kg$ (mean${\pm}$standard deviation) were grouped in a randomized complete block design into eight blocks of five animals each based on their initial body weight. Straws included in the study were from Mosisa (T1M), Walki (T2W), Degaga (T3D), Shallo (T4S), and local (T5L) varieties of faba bean and concentrate (2:1 ratio of wheat bran to "noug" seed cake). The experiment consisted of seven days of digestibility and 90 days of feeding trials followed by evaluation of carcass parameters at the end. Results: Local variety had lower (p<0.05) in grain and straw yield compared to improved varieties but higher in crude protein, metabolizable energy contents and in vitro organic matter digestibility. The apparent digestibility of dry matter and crude protein of sheep fed Walki and Mosisa straws were higher than (p<0.05) straws from Shallo varieties. Sheep fed Walki straw had greater (p<0.05) dry matter intake, average daily gain and feed conversion efficiency than sheep fed local and Shallo straws. Slaughter body weight and empty body weight were higher (p<0.05) for sheep fed Mosisa and Walki straws as compared to sheep fed Shallo straws. Apart from this, the other carcass components were not affected (p>0.05) by variety of the faba bean straws. Conclusion: There is significant varietal differences between faba bean straws both in quality and quantity. Similarly, significant variation was observed in feed intake, digestibility, body weight gain and feed conversion efficiency among sheep fed different straws of faba bean varieties with concentrate supplement. Based on these results, Walki and Mosisa varieties could be recommended as pulse crop rotation with cereals in the study area.

Lycopene Content and Fruit Morphology of Red, Pink, Orange, and Yellow Fleshed Watermelon (Citrullus lanatus) Germplasm Collections

  • Noh, Jae-Jong;Hur, On-Sook;Ro, Na-Young;Lee, Jae-Eun;Hwang, Ae-Jin;Kim, Bit-Sam;Rhee, Ju-hee;Yi, Jung Yoon;Kim, Ji Hyun;Lee, Ho-Sun;Sung, Jung-Sook;Kim, Myung-Kon;Assefa, Awraris Derbie
    • Korean Journal of Plant Resources
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    • 제33권6호
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    • pp.624-637
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    • 2020
  • High-quality and high-phytonutrient watermelon fruits have strong market opportunities besides their health related benefits. Hence, investigating quality and nutritional related traits of watermelon genetic resources could provide important baseline data in breeding for increased lycopene content thereby increasing the marketability of watermelon. To this end, we have examined some fruit morphological traits and lycopene content of 105 genetic resources. Seeds, originally obtained from 22+ countries, were obtained from the National Agrobiodiversity Center, Jeonju, South Korea, grown in an experimental field and harvested at a fully mature stage. The size of pistil scar (SPS), the width of stripes (WS), weight of fruit (WF), length of fruit (LF), width of fruit (WIF), the thickness of pericarp (TP), soluble solids content (SSC), fruit shape in longitudinal section, ground color of skin, the intensity of the green color of skin, fruit shape at the apical part, grooving distribution, conspicuousness of stripes, and main color of the flesh were recorded on the field and inside laboratory and the lycopene was measured using spectrophotometric and HPLC methods. Watermelon fruits have shown a diverse morphological characters. Red and pink fleshed fruits dominated in the entire collections. Fruits with higher thickness of rind were found to exhibit less soluble solid content (SSC). Korean origin fruits were characterized by intermediate SSC while the United States of America (USA), Russia (RUS), Tajikistan (TJK), Turkmenistan (TKM), Taiwan (TWN), and Uruguay (URY) originated fruits had the highest SSC. The lycopene content varied between 41.37 and 182.82 ㎍/g, 2.81 and 163.72 ㎍/g, and 3.54 and 255.47 ㎍/g using HPLC, UV-Vis spectrophotometer, and microplate reader spectrophotometer, respectively. Red- and pink-fleshed fruits had the highest levels of lycopene content compared to the yellow- and orange-fleshed. Lycopene content had a significant positive correlation with SSC, however, no correlations were detected between lycopene and other quantitative fruit morphological characters. Our study demonstrated high diversity exists in fruit morphological traits and lycopene content of the germplasm collections which provide beneficial baseline data for a future breeding program and utilization of watermelon germplasm collections in gene banks for the maintenance and improvement of the current levels of production, marketability, and health-related benefit of watermelon fruits.

Bacteriological profiles of dressed broilers at different conditions and frozen storage periods

  • Ehsan, M.A.;Rahman, M.S.;Chae, Joon-Seok;Eo, Seong-Kug;Lee, Ki-Won;Kim, In-Shik;Yoon, Hyun-A;Lee, John-Hwa
    • Korean Journal of Veterinary Research
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    • 제44권2호
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    • pp.225-231
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    • 2004
  • This study was conducted to determine the incidence of microorganisms associated with dressed broiler with intact skin and without skin at different frozen storage periods such as 0, 10, 20, 30 days and to demonstrate the role of packaging and pretreatment chilling on the changes of carcass quality. The values of total viable count (TVC), total coliform count (TCC), total streptococcal count (TStC) and total staphylococcal count (TSC) were determined for meat samples of thigh and breast and swab samples of visceral surfaces of the broilers with intact skin and without skin. It was observed that the values of TVC, TCC, TStC and TSC in both cases of dressed broiler with intact skin and without skin exceeded the International Commission on Microbiological Specification for Foods. However, numbers of microorganisms were considerably decreased during the frozen storage. Packing and prechilled conditions were generally better effective in decrease of the loads of microorganisms than without packing and prechilled conditions, and lower bacterial numbers were also found in dressed broiler with intact skin than that without skin. The highest sensory panel score was obtained at 10 days of frozen storage. These results, thus, indicate that usages of appropriate periods and conditions of frozen storage and packaging systems can minimize the potential health hazards associated with contaminants gaining access to the dressed or processed broilers and improve the quality and shelf life of dressed broilers.

Influence of Weather Factors on the Incidence of the Mulberry Aleyrodid, Dialeuropora decempuncta (Quaintance and Baker) and Their Relation to Yield Loss

  • Bandyopadhyay U. K.;Santhakumar M. V.;Sahu P. K.;Saratchandra B.
    • International Journal of Industrial Entomology and Biomaterials
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    • 제11권2호
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    • pp.129-133
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    • 2005
  • The seasonal occurrence and influence of abiotic factors viz., maximum and minimum temperature, maximum and minimum humidity and rainfall on population fluctuation of aleyrodid, Dialeuropora decempuncta on a evolved mulberry (Morus alba L.) variety known to be susceptible to aleyrodid infestation was assessed during the period from 1999 - 2001 in twenty-five villages under nine blocks of Malda district of West Bengal. The results indicate that the aleyrodid population is practically very low or absent during January to June and thereafter increases gradually. The increase in population of various stages of aleyrodid is significantly correlated with increase in previous 7 days of average maximum relative humidity.