한국국제농업개발학회지 (Journal of the Korean Society of International Agriculture)

  • 제30권4호
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  • Pages.357-369
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  • 2018
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  • 1225-8504(pISSN)
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  • 2287-8165(eISSN)
한국국제농업개발학회 (The Korean Society of International Agriculture)
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한국 재래종 강낭콩 유전자원의 phytochemical 및 항산화 활성 평가

Evaluation of Phytochemical econtents and antioxidant activity of Korean common bean (Phaseolus vulgaris L) landraces

  • 이경준 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 신명재 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 조규택 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이기안 (농촌진흥청 연구정책과) ;
  • 마경호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정종욱 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 이정로 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Lee, Kyung Jun (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Shin, Myoung-Jae (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Cho, Gyu-Taek (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) ;
  • Lee, Gi-An (Research Policy Bureau, RDA) ;
  • Ma, Kyung-Ho (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), RDA) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Lee, Jung-Ro (National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA)
  • 투고 : 2018.08.17
  • 심사 : 2018.12.14
  • 발행 : 2018.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

1. 본 연구는 한국 재래종 강낭콩 209자원의 phytochemical 및 항산화활성을 평가하였다. 2. 항산화활성은 DPPH, ABTS, FRAP, SOD를 분석하였으며 phytochemical은 kaempferol, myricetin, quercetin, naringenin 함량을 각각 분석하였다. 3. 항산화활성은 강낭콩 자원 간 다양한 분포를 보였으며 DPPH의 경우 62.3~643.9 (IC50), ABTS의 경우 0.28~1.49 mgAAE/g, FRAP의 경우 0.41~5.44 mgAAE/g, SOD의 경우 50.4 ~ 299.8 (IC50)로 나타났다. 4. Relative antioxidant capacity index (RACI)로 강낭콩 자원의 항산화활성을 비교한 결과 IT104587이 가장 높은 항산화활성을 보였으며 IT189598이 가장 낮은 항산화활성을 보였다. 5. 분석된 Phytochemical 중에서 한국 재래종 강낭콩에서는 Kaempferol이 가장 높은 함량을 나타냈다. 6. PCA 분석 결과 209자원은 3개의 그룹으로 나뉘었으며 이중 그룹 III에 속한 46자원의 강낭콩이 낮은 항산화활성 및 phytochemical 함량을 보였다. 7. 본 연구 결과는 한국 재래종 강낭콩의 항산화활성 및 phytochemical 정보를 제공하며 이 정보는 강낭콩 품종 개발을 위한 기초 정보로 사용될 수 있을 것이다.

The Korean common bean (Phaseolus vulgaris L.) has been receiving increased attention as a functional food. The objective of this study was to reveal the phytochemicals genetic variation and antioxidant activity of 209 Korean common bean landraces. Antioxidant activity was evaluated with the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power (FRAP), and superoxide dismutase (SOD) assay. Antioxidant activities among common bean accessions showed wide variation. Four flavonoids (kaempferol, myricetin, quercetin, and naringenin) of the 209 Korean common bean landraces were measured using HPLC. Among them, kaempferol had the highest phytochemicals compared to the other three flavonoids. Using the relative antioxidant capacity index (RACI), it was found out that the IT104587 had the highest antioxidant activity. Meanwhile, in clustering analysis, the Korean common bean landraces were classified into three clusters. Among them, cluster II contained 64 landraces with higher antioxidant activities and phytochemicals than the other clusters, except DPPH. The results could provide information on the valuable Korean common bean landraces for the development of new common bean varieties.

키워드

과제정보

연구 과제번호 : Research Program for Agricultural Science & Technology Development

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