• Title/Summary/Keyword: International Agricultural Research Institute

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Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae

  • Kim, Jae-Su;Roh, Jong-Yul;Choi, Jae-Young;Shin, Sang-Chul;Jeon, Mun-Jang;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.17 no.2
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    • pp.211-215
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    • 2008
  • To select entomopathogenic fungi controlling aphids effectively, several isolates were screened against second instars of Myzus persicae nymphs in the glasshouse using conidia suspension at $1.0{\times}10^5$ conidia/ml. Among these isolates, SFB-205 conidia showed the highest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface of M. persicae nymphs, and germination and penetration were observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana species through the comparison of 5.8 s rRNA genes. There were 24 polymorphisms between SFB-205 and the previously reported isolate, B. bassiana ATCC74040 using six kinds of primer combinations in amplified fragment length polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biological control agent for the green peach aphid, M. persicae in the field.

Deproteinized Mulberry Leaf Juice - A New Media for Growth of Microorganisms

  • Chowdary, N.B.;Naik, V.Nishitha;Sharma, D.D.;Govindaiah
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.217-220
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    • 2002
  • Mulberry being a foliage crop is grown extensively for feeding of silkworms and are also used for cattle feeding. These loaves are highly nutritious, which contain various mineral elements and bio-molecules such as carbohydrates, proteins, lipids and other essential amino acids, etc. In the present study, deproteinized mulberry leaf juice was used for preparation of the medium for cultivation of various types of microbes. Results revealed that deproteinized mulberry leaf juice medium is best for isolation of fungi, bacteria and actinomycetes and this medium can be substituted with synthetic media, which are haying the costly ingredients for isolation and identification of bacteria, fungi and actinomycetes. Further, this deproteinized mulberry juice medium can also be used in mass multiplication of useful/beneficial microbes to enhance soil microflora to improve soil fertility and to avoid root diseases. Perspective enterprises can take up the mass multiplication/large-scale production of useful microbes such as Trichoderma, Rhizobium, Pseudomonas and Bacillus to use in mulberry and in other agricultural crops using deproteinized mulberry leaf juice.

Food Ingestion, Assimilation and Conversion Efficiency of Mulberry Silk­worm, Bombyx mori L.

  • Rahmathulla V. K.;Haque Rufaiel S. Z.;Himantharaj M. T.;Vindya G S.;Rajan R. K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.1
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    • pp.1-12
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    • 2005
  • Feed conversion efficiency contributes directly or indirectly on the cost benefit ratio of silkworm rearing and is considered to be an important physiological criterion for evaluating the superiority of silkworm breeds/hybrids. Food intake, assimilation and conversion of indigenous as well as exotic silkworm races are well studied by many researchers. In this review, an attempt has been made to consolidate works on feed conversion aspects of indigenous and exotic silkworm races. The paper also deals with the effect of various factors viz., nutritional, environmental and feeding on food assimilation and conversion parameters of mulberry silkworm.

Genetic resources of sweetpotato for industrial use (산업용 고구마 개발을 위한 유전자원 현황 및 전망)

  • Mok, Il-Gin;Zhao, Donglan;Kwak, Sang-Soo
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.202-206
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    • 2009
  • In many countries including China and U.S., researchers are developing methods to use sweetpotato as raw material for biofuel. We consider the sweetpotato is not only a source of green fuel, it eventually will provide various material including paper, adhesives, biodegradable plastics, and secondary metabolites. Sweetpotato is one of the high efficiency crop because it yields more calories per unit area than either maize or potato, and it requires the shortest growing cycle of the root crops grown in the tropics. Sweetpotato is the most useful crop for the coming starchbased industry era. Sweetpotato genetic resources are collected, characterized, evaluated, and maintained by U.S., China, Japan, and the International Potato Center. New varieties of sweetpotato using the proper genetic resources and molecular breeding will be developed to cope with the global food and energy in 21st century.

Screening of Resistance of Introduced Kimchi Cabbage (Brassica rapa subsp. pekinensis) Germplasm from Asian areas to Plasmodiophora brassicae Isolates Collected in Korea. (배추 아시아 도입 유전자원의 국내 재배포장에서 수집한 뿌리혹병 균주에 대한 저항성 반응)

  • Jeon, Young-Ah;Lee, Ho-Sun;Rhee, Ju-Hee;Lee, Jae-Eun;Ko, Ho-Cheol;Aseefa, Awraris Derbie;Sung, Jung-Sook;Hur, On-sook;Ro, Na-young;Lee, Sok-Young
    • Journal of the Korean Society of International Agriculture
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    • v.30 no.4
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    • pp.305-312
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    • 2018
  • Clubroot, caused by Plasmodiophora brassicae, is one of the most crucial disease in Kimchi cabbage. Screening disease resistant genetic resources is necessary to develop disease resistant cultivars and conduct related research. We have evaluated the development of clubroot to the 120 genetic resources of Kimchi cabbage introduced from World Vegetable Center and five Asian countries using isolate of Plasmodiophora brassicae collected in Haenam fields in Jeollanam-do Province, Rep. of Korea. This isolate was determined race 2 using differential varieties reported by Kim et al., 2016. IT100384 and IT305623 showed strong resistance, lower than disease index (DI) 1.0. IT100385, 100439, and 135407 showed moderate resistance (1.0

Algebraic Analysis for Partitioning Root and Stem Lodging in Rice Plant

  • Chang, Jae-Ki;Yeo, Un-Sang;Lee, Jeom-Sig;Oh, Byong-Geun;Kim, Jeong-Il;Yang, Sae-Jun;Ku, Yeon-Chung;Kim, Ho-Yeong;Sohn, Jae-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.539-543
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    • 2006
  • Lodging is classified as root lodging caused by the loss of supporting force in the root, bending caused by the deformation of the stem and breaking where the stem breaks down as loads exceeding critical elasticity were applied. This research excluded breaking which is not in a state of equilibrium and tried to partition the level of lodging using an algebraic model in root lodging and stem lodging, or bending. When a vertical load was applied, the deformation of the stem of rice plant showed the form of a quadratic equation. The trace of the panicle neck in the process of lodging was an ellipse-shape. When loading was pure root lodging, the trace of the panicle neck became a circle of which culm length is the radius. When it was a pure stem lodging, the trace of the panicle neck is an ellipse of which major axis is culm length and minor axis is 0.64* culm length. When both stem lodging and root lodging occurred in a natural setting, the partitioning of lodging can be calculated by a formula using eccentricity of an ellipse, S=e*100/0.768(S is the ratio of stem lodging in the whole lodging, e is eccentricity of the ellipse). This method is expected to be useful in simple lodging partitioning. We could also calculate the partitioning of stem lodging and root lodging as units of angles as an accuracy method, by using a straight line calculated by differentiating a quadratic equation of stem deformation at the origin of the coordinates. These two methods for dividing root and stem lodging showed different values. However, each of them showed almost same values with different lodging degree in one plant.

An Effect of Information System Quality of BRIS on Perceived Usefulness and User's Continuous Use Intention

  • Kim, Sang-tae;Lee, Seung-hyeon;Lee, Kang-o
    • Agribusiness and Information Management
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    • v.11 no.2
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    • pp.16-24
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    • 2019
  • BRIS has been established by the Ministry of Agriculture, Food and Rural Affairs since 2011 under Article 21 of the Act on the Preservation, Management and Utilization of Agricultural Life Resources. It is an information service that provides information related to agricultural life resources in connection with the Rural Development Administration, the Forest Service, and Korea Seed & Variety Service, Agricultural Resources and Agricultural and Livestock Quarantine Headquarters. The purpose of this study is to evaluate how the users assess the information system quality (information quality, system quality) for the current Bio Resource Information Service (BRIS). Ultimately, the structural equation modeling analyzes the causal relationship between each variable and how it responds to the user's continuous use intention. As a result, information quality among information system quality and information quality among system quality used as the main variables for the evaluation of BRIS were statistically positively influenced by users' perceived usefulness, while system quality It has been analyzed to have a positive effect on the sustained use intention. The user's continuous use intention is positively influenced by the information system quality and perceived usefulness at a statistically significant level. Especially, the perceived usefulness plays a role in mediating the user's continuous use intention.

Development of GASS2 through Improving Inter-component Connection and Communication Modules (연결성과 소통구조 모듈을 통한 차세대 범용 농업시스템 시뮬레이터 (GASS2)의 개발)

  • Kim, Tae Gon;Lee, Sung Yong;Yi, Ho Jae;Lee, Jeong Jae;Suh, Kyo
    • Journal of The Korean Society of Agricultural Engineers
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    • v.56 no.5
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    • pp.29-36
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    • 2014
  • The purpose of this study is to improve the Generic Agricultural Systems Simulator (GASS) which can simulate various rural systems based on object-oriented model. GASS provides the configuration platform of various system components to simplify integrated agricultural system models such as irrigation systems for rice farming. The new connection and communication modules of GASS improve applicability for modelling diverse systems. The geometric connection of GASS replaces topological connection, and communication protocols expand to analyze not only homogeneous system but also heterogeneous system. In this paper, we applied GASS2 to simulate the water heights of linked tanks and the simulation outputs were verified through comparing with analytical solutions of differential equations. The two new modules make it possible to analyze the 4-tank problem which includes topological and heterogenous issues with GASS2.

Survey of Microbial Contamination of Tomatoes at Farms in Korea (국내 생산단계 토마토의 위생지표세균 및 유해미생물 오염도 조사)

  • Kim, Won-Il;Jo, A-Ra;Lee, Ju-Han;Kim, Se-Ri;Park, Kyeong-Hun;Nam, Ki-Woong;Yoon, Yohan;Yoon, Deok-Hoon;Oh, So-Yong;Lee, Mun Haeng;Ryu, Jae-Gee;Kim, Hwang-Yong
    • Journal of Food Hygiene and Safety
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    • v.28 no.4
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    • pp.324-329
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    • 2013
  • This study investigated and evaluated contamination levels of bacteria on tomatoes at farms stage to evaluate potential hazards associated with fresh tomatoes. A total of 170 samples, 90 samples from 5 sampling sites from 18 farms and 80 samples from 1 sampling site from 4 farms every month for four months, were analyzed to enumerate aerobic bacterial counts, coliforms, E. coli, Bacillus cereus, Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes. Aerobic bacterial counts ranged from 0.48 to 6.15 Log CFU/g, with the lowest and the highest bacterial cell counts recorded for A site and E site, respectively. Thirty five percent of the samples from the E site contained more than 2 Log CFU/g. Six samples (6.6%) of 90 samples contained B. cereus less than 1 Log CFU/g. In addition, the contamination level of indicator bacteria and B. cereus in tomatoes were higher on March than on April, May and June (P < 0.05). S. aureus, E. coli, E. coli O157:H7, Salmonella spp. and L. monocytogenes were not detected in the tomato samples. The microbial contamination levels of tomatoes determined in this study may be used as the data for microbiological risk assessment.

Determination of Optimized Growth Medium and Cryoprotective Additives to Enhance the Growth and Survival of Lactobacillus salivarius

  • Yeo, Soyoung;Shin, Hee Sung;Lee, Hye Won;Hong, Doseon;Park, Hyunjoon;Holzapfel, Wilhelm;Kim, Eun Bae;Huh, Chul Sung
    • Journal of Microbiology and Biotechnology
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    • v.28 no.5
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    • pp.718-731
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    • 2018
  • The beneficial effects of lactic acid bacteria (LAB) have been intensively investigated in recent decades with special focus on modulation of the host intestinal microbiota. Numerous discoveries of effective probiotics are driven by a significantly increasing demand for dietary supplements. Consequently, technological advances in the large-scale production and lyophilization are needed by probiotic-related industries for producing probiotic LAB for commercial use. Our study had a dual objective, to determine the optimum growth medium composition and to investigate appropriate cryoprotective additives (CPAs) for Lactobacillus salivarius, and compare its responses with other Lactobacillus species. The one-factor-at-a-time method and central composite design were applied to determine the optimal medium composition for L. salivarius cultivation. The following composition of the medium was established (per liter): 21.64 g maltose, 85 g yeast extract, 1.21 ml Tween 80, 6 g sodium acetate, $0.2g\;MgSO_4{\cdot}7H_2O$, $0.02g\;MnSO_4{\cdot}H_2O$, $1g\;K_2HPO_4$, $1.5g\;KH_2PO_4$, $0.01g\;FeSO_4{\cdot}7H_2O$, and 1 g sodium citrate. A cryoprotective additive combination comprising 10% (w/v) skim milk and 10% (w/v) sucrose supplemented with 2.5% (w/v) sodium glutamate was selected for L. salivarius, and its effectiveness was confirmed using culture-independent methods in the freeze-dried cells of the Lactobacillus strains. In conclusion, the optimized medium enhanced the species-specific cultivation of L. salivarius. On the other hand, the cryoprotective effects of the selected CPA mixture may also be dependent on the bacterial strain. This study highlights the necessity for precise and advanced processing techniques for large-scale production of probiotics in the food and feed industries.