Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
권호 표지
DOI QR코드

DOI QR Code

Screening of Resistance of Introduced Kimchi Cabbage (Brassica rapa subsp. pekinensis) Germplasm from Asian areas to Plasmodiophora brassicae Isolates Collected in Korea.

배추 아시아 도입 유전자원의 국내 재배포장에서 수집한 뿌리혹병 균주에 대한 저항성 반응

  • Jeon, Young-Ah (International Technology Cooperation Center, Rural Development Administration) ;
  • Lee, Ho-Sun (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Rhee, Ju-Hee (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lee, Jae-Eun (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Ko, Ho-Cheol (Client Service Division, Rural Development Administration) ;
  • Aseefa, Awraris Derbie (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sung, Jung-Sook (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hur, On-sook (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Ro, Na-young (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lee, Sok-Young (National Agrobiodiversity Center, National Institute of Agricultural Science, Rural Development Administration)
  • 전영아 (농촌진흥청 기술협력국 국제기술협력과) ;
  • 이호선 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이주희 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이재은 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 고호철 (농촌진흥청 기획조정관실 고객지원담당관실) ;
  • 아와리스 아세파 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 성정숙 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 허온숙 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 노나영 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이석영 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Received : 2018.09.21
  • Accepted : 2018.12.07
  • Published : 2018.12.31
⟨ Previous Next ⟩

Abstract

Clubroot, caused by Plasmodiophora brassicae, is one of the most crucial disease in Kimchi cabbage. Screening disease resistant genetic resources is necessary to develop disease resistant cultivars and conduct related research. We have evaluated the development of clubroot to the 120 genetic resources of Kimchi cabbage introduced from World Vegetable Center and five Asian countries using isolate of Plasmodiophora brassicae collected in Haenam fields in Jeollanam-do Province, Rep. of Korea. This isolate was determined race 2 using differential varieties reported by Kim et al., 2016. IT100384 and IT305623 showed strong resistance, lower than disease index (DI) 1.0. IT100385, 100439, and 135407 showed moderate resistance (1.0

배추의 뿌리혹병 저항성 품종 개발 및 관련 연구에 활용할 수 있는 자원 탐색을 목적으로 세계채소센터(World Vegetable Center, 대만 소재)와 아시아 지역 5개국에서 도입한 120 유전자원을 대상으로 전라남도 해남군 배추 재배포장에서 발병한 뿌리혹병균(레이스 2)을 접종하여 저항성 반응을 조사함 1. 발병정도가 1 이하로 저항성 반응을 보인 자원은 IT100384와 IT305623로 2개이고, 발병정도가 1 초과 2 미만으로 중도 저항성 반응을 보인 자원은 IT100385, 100439, 135407로 3개임. 그 외 자원은 발병정도가 2 이상으로 감수성 반응을 보임 2. 저항성 반응을 보인 IT100384는 발병정도는 0.3으로 거의 증상을 보이지 않았으며, IT305623은 발병한 식물체가 발견되지 않아 가장 저항성이 높은 자원으로 나왔음 3. 뿌리혹병균에 대해 감수성 반응을 보인 자원들 중 발병 정도가 4.0으로 병 증상이 아주 심한 자원이 IT100349 등 23개이고, 발병정도가 3.0에서 4.0 미만인 자원이 84개로 가장 많았고, 2.0에서 3.0 미만인 자원은 8개임 4. 본 연구결과 배추 뿌리혹병 발병정도가 낮아 저항성 및 중도 저항성 자원으로 예측되는 5개 자원에 대해서, 병 검정이 유묘 단계에서 이루어졌고 한 개의 균으로 검정하였기 때문에 향후 다양한 균주를 이용하여 재평가함으로 활용가치가 높은 유전자원을 제공할 수 있도록 계속 연구 필요

Keywords

References

  1. Akaba, M., Y. Kaneko, K. Hatakeyama, M. Ishida, S. W. Bang, and Y. Matsuzawa. 2009. Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line. Breeding Science 59:203-206. https://doi.org/10.1270/jsbbs.59.203
  2. Cheah, L. H. and Veerakone, S. and Kent, G. 2000. Biological control of clubroot on cauliflower with Trichoderma and Streptomyces spp. N. Z. Plant Protection. 53:18-21. https://doi.org/10.30843/nzpp.2000.53.3642
  3. Cho, K. S., Y. H. Han, J. T. Lee, E. J. Hur, T. J. Yang, and J. G. Woo. 2002. Pathogenic differentiation of Plasmodiophora brassicae and selection of Chinese cabbage cultivars resistant to clubroot disease in highland. Korean J. Breed. 34(3):168-173.
  4. Cho, W. D., W. G. Kim, and K. Takahashi. 2003. Occurrence of clubroot in cruciferous vegetable crops and races of the pathogen in Korea. Plant Pathol. J. 19:64-68. https://doi.org/10.5423/PPJ.2003.19.1.064
  5. Crute, I. R. and D. A. C. Pink. 1989. The characteristic and inheritance of resistance to clubroot in Brassica oleracea. Aspect. Appl. Biol. 23:57-60.
  6. Jo, E. J., K. S. Jang, Y. H. Choi, K. G. Ahn, G. J. Choi. 2016. Resistance of cabbage plants to isolates of Plasmodiophora brassicae. Korean J. Hortic. Sci. Technol. 34(3):442-452.
  7. Jo, S. J., K. S. Jang, Y. H. Choi, J. C. Kim, and G. J. Choi. 2010. Convenient screening method of Chinese cabbage for resistance to Plasmodiophora brassicae using soil-drenching inoculation. Res. Plant Dis. 16(3):279-284. https://doi.org/10.5423/RPD.2010.16.3.279
  8. Jo, S. J., K. S. Jang, Y. H. Choi, J. C. Kim, and G. J. Choi. 2011. Development of convenient screening method for resistant radish to Plasmodiophora brassicae. Res. Plant Dis. 17(2):161-168. https://doi.org/10.5423/RPD.2011.17.2.161
  9. Jo, S. J., S. A. Shim, K. S. Jang, Y. H. Choi, J. C. Kim, and G. J. Choi. 2011. Resistance of cultivars of Chinese cabbage to Plasmodiophora brassicae isolates of several races collected in Korea. Kor. J. Hort. Sci. Technol. 29(6):610-616.
  10. Karing, J. S. 1968. The plasmodiophorales. Hafner Publ. Co., New York, USA p. 239.
  11. Kim, C. H., W. D. Cho, and S. B. Lee. 2003. Review of researches on clubroot disease of Chinese cabbage in Korea and future tasks for its management. Res. Plant Dis. 9(2):57-63. https://doi.org/10.5423/RPD.2003.9.2.057
  12. Kim, H., E. J. Jo, Y. H. Choi, K. S. Jang, and G. J. Choi. 2016. Pathotype classification of Plasmodiophora brassicae isolates using clubroot-resistant cultivars of Chinese cabbage. Plant Pathol. J. 32(5):423-430. https://doi.org/10.5423/PPJ.OA.04.2016.0081
  13. Korea Seed & Variety Service. 2018. URL:http://seed.go.kr.Statistics.
  14. Kuginuki, Y., H. Yoshikawa, and M. Hirai. 1999. Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot resistance cultivars of Chinese cabbage(Brassica rapa L. ssp. pekinensis). Eur. J. Plnat Pathol. 105:327-332. https://doi.org/10.1023/A:1008705413127
  15. Lee, M.G. W.S. Park. D. H. Byun. 1995. A study on the English marking of Kimchi made of original chinese cabbage. Food Science and Industry.28:2-5.
  16. Ministry of Agriculture, Food and Rural Affairs. 2018. Current status of vegetables' production in 2017. p. 19.
  17. Mitani, S., K. Sugimoto, H. Hayashi, Y. Takii, T. Ohshima, and N. Matsuo. 2003. Effects of cyazofamid against Plasmodiophora brassicae Woronin on Chinese cabbage. Pest Mnag. Sci. 59:287-293. https://doi.org/10.1002/ps.627
  18. Murakami, H., S. Tsushima, T. Akimoto, K. Murakami, I. Goto, and Y. Shishido. 2000. Effects of growing leafy daikon(Raphanus sativus) on population of Plasmodiophora brassicae(Clubroot). Plant Pathol. 49:584-589. https://doi.org/10.1046/j.1365-3059.2000.00495.x
  19. Rural Development Administration, 2018. Statistics of Rural Development Business. pp 91-94.
  20. Soh, J. W., K.S. Han, S. C. Lee, and J. H. Park. 2014. Contamination of Plasmodiophora brassicae on soil in highland area of Chinese cabbage cultivation. Korean J. Int. Agric. 26:157-161. https://doi.org/10.12719/KSIA.2014.26.2.157
  21. Suwabe, K., H. Tsukada, H. Iketani, K. Hatakeyama, M. Fujimura, T. Nunome, H. Fukuoka, S. Matsumoto, and M. Hirai. 2003. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theor. Appl. Genet. 107:997-1002. https://doi.org/10.1007/s00122-003-1309-x
  22. Tanaka, S., S. Fujiyama, S. Shigemori, A. Nakayama, S. Ito, and I. M. Kameya. 1998. Pathogenesis of isolates of Plasmodiophora brassicae from Japan. (1) Race and pathogenesis in clubroot resistant cultivars. Proc. Assoc. Plant Protect. Kyushu 44:15-19. https://doi.org/10.4241/kyubyochu.44.15
  23. Voorrips, R. E. 1996. Production and characterization of single-spore isolates of Plasmodiophora brassicae. Eur. J. Plant Pathol. 102:377-383. https://doi.org/10.1007/BF01878132