• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.36-36
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• 2023

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.37-37
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• 2023

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.1
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• pp.31-35
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• 2016

The influence of commonly used steeping times and temperatures about mulberry tea on a-glucosidase inhibitory activity were studied. The effects of product and preparation variables on the in-cup chemical composition of mulberry tea extracts is of interest because the appearance and taste characteristics and the possible health effects of a tea liquor arise from the chemical components extracted from the leaf during mulberry tea preparation. A comprehensive study was therefore undertaken to determine the contributions of product and provide a basic data for development of high quality mulberry tea products against diabetes. Specific eight mulberry tea were collected from each region of Korea (A-I) and when it was extracted on 85℃ for 2min, a-glucosidase activity was best for 98-102%. According to various temperature and time extraction methods, this study was carried out to optimize teamaking conditions for maximal DNJ extraction from Korean mulberry tea and and it would be of particular interest for people who drink mulberry tea to control blood glucose levels.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.137-141
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• 2011

A previous data was provided information for tissuespecific expression genes by means of whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the hemocyte tissue on 5 days of 5th instar larvae during the development of $B.$ $mori$. Total 5 candidates pick out from the $Bombyx$ $mori$ Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray). To verify the hemocyte-specific expression, we analyzed by semi-quantitative and real-time quantitative RT-PCR using the highly expressed endogenous $Actin$ RNA as an intrinsic reference. In this study, we confirmed that one gene-sw17255- out of 5 candidates expressed in the hemocyte tissue, which was consistent with the previous data. Circulating hemocytes in the body fluid of the $B.$ $mori$ are most powerful target organ for producing biomaterials. We need further studies to find hemocyte-specific promoter region from sw17255 gene. Finally, this result can be applied in creating transgenic silkworms as a biomedical insect.

• Journal of The Korean Society of Agricultural Engineers
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• v.51 no.2
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• pp.35-41
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• 2009

The impacts of climate change on paddy irrigation requirements for Nakdong river basin in Korea have been analyzed. The HadCM3 model outputs for SRES A2 and B2 scenarios and International Water Management Institute $10'{\times}10'$ pixels observed data were used with kriging method. Maps showing the predicted spatial variations of changes in climate parameters and paddy irrigation requirements have been produced using the GIS. The results showed that the average growing season temperature was projected to increase by $2.2^{\circ}C$ (2050s A2), $0.0^{\circ}C$ (2050s B2), $3.7^{\circ}C$ (2080s A2) and $2.9^{\circ}C$ (2080s B2) from the baseline (1961-1990) value of $21{\circ}C$. The average growing season rainfall was projected to increase by 15.2% (2050s A2), 24.2% (2050s B2), 41.4% (2080s A2) and 16.7% (2080s B2) from the baseline value of 900 mm. Average volumetric irrigation demands were projected to decrease by 3.7% (2050s A2), 7.0% (2050s B2), 10.2% (2080s A2) and 1.4% (2080s B2) from the baseline value of $1.25{\times}10^9\;m^3$. These results can be used for the agricultural water resources development planning in the Nakdong river basin for the future.

• Journal of Environmental Science International
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• v.24 no.4
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• pp.515-524
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• 2015

The estimation of groundwater usage in Jeju island is important to understand hydrologic cycle system and to plan management of water resource because large amounts of groundwater have been used for agricultural and domestic purpose. The model has been developed to estimate agricultural groundwater usage for garlic at uplands and citrus at orchards raising outdoors using the soil water balance model from FAO 56, respectively. The total amount of water supplied for the crop evapotranspiration and the multipurpose function such as sprout promotion can be simulated by the model. However, due to the discrepancy of water use in initial stage between calculated and observed, the model was calibrated and verified using actual groundwater usage monitoring data for 3.5 years (2011.6 to 2014.12) at three uplands for garlic and three orchards for citrus. Consequently, it would be concluded that the model simulated efficiently actual water usage in that root mean square (RMS) and normalized RMS of the validation stage were less than 8.99 mm and 2.43%, respectively, in two different conditions.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.118-122
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• 2017

Porcine pseudorabies virus (PRV) causes the Aujeszky's disease (AD) which is economically important disease in the swine industry worldwide. Killed or live vaccines have been used to control this disease, but their efficacy and side effects remain problems to be solved. To solve these problems, in this study, production of recombinant PRV glycoprotein gB, gC and gD was investigated in insect expression system. Glycoprotein gB, gC and gD are regarded as the major immunogenic antigens in PRV. Abundant production and immunogenicity of glycoprotein gB, gC and gD were confirmed by SDS-PAGE and Western blot analysis, respectively. Optimal infection dose and time were also determined for the production of each recombinant PRV glycoprotein. Confirmation of glycosylation of recombinant gB, gC and gD suggested their usefulness as antigens for the development of diagnosis kit or vaccines for Aujeszky's disease.

• Research in Plant Disease
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• v.29 no.2
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• pp.108-117
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• 2023

With the development of advanced virus diagnostic technologies, numerous viruses, including novel viruses, have been identified explosively from various biological samples around the world over the last decade. For plant viruses, approximately 376 novel viruses have been reported in the last three years. Information on the occurrence and host ranges of plant viral diseases in a particular country or region is very important for diagnosis, quarantine, and control of the viral diseases. Recently, based on active research on the diagnosis and identification of plant viruses, a significant number of newly occurring viruses and new viral host plants have been reported in South Korea. This review paper provides integrated information on plant viruses and viral host plants newly reported in South Korea between 2020 and 2022 to help diagnose, control, and quarantine plant virus diseases in crop fields.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.87-91
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• 2005

We have first isolated and characterized a Plutella xylostella granulovirus ($P_xGV$) from dead larvae of P. xylostella in Korea. The granule of $P_xGV$ was ovoidal shape with an approximate measure of $350-400nm{\times}150-200nm$, and each granule contained one single rod-shape virion with a mean size of $150-180nm{\times}20-30nm$. The major granule protein, granulin, had a molecular weight of approximately 29kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported $P_xGV$, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported $P_xGV$. These results suggested that the $P_xGV$ isolated in this study was a novel isolate containing different genomic information.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.2
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• pp.101-105
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• 2011

Transparency and insolubility of eardrum patch against exudates are important for otolaryngological surgery. The author prepared silk fibroin (SF) films with various concentrations and temperature and then examined solubility and conformation of SF films. SF films were transparent regardless of the various preparation conditions. Although most SF films are soluble in 1X PBS solution at $37^{\circ}C$ for 1 h, the SF film with 3.4% with $60^{\circ}C$ was insoluble. Scanning electron microscopy (SEM) showed that the SF films have solid and smooth surface. Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) results showed that the conformation of SF films was influenced by the preparation conditions including SF concentration and casting temperature. In conclusion, SF membrane with transparence and insolubility against exudates could be considered as eardrum patch resources.