International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Expression of Pseudorabies Virus Glycoproteins gB, gC and gD using Insect Expression System

  • Yun, Bit Na Rae (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Gwak, Won Seok (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Lee, Ji Hoon (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Woo, Soo Dong (Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • Received : 2017.11.10
  • Accepted : 2017.12.12
  • Published : 2017.12.31
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Abstract

Porcine pseudorabies virus (PRV) causes the Aujeszky's disease (AD) which is economically important disease in the swine industry worldwide. Killed or live vaccines have been used to control this disease, but their efficacy and side effects remain problems to be solved. To solve these problems, in this study, production of recombinant PRV glycoprotein gB, gC and gD was investigated in insect expression system. Glycoprotein gB, gC and gD are regarded as the major immunogenic antigens in PRV. Abundant production and immunogenicity of glycoprotein gB, gC and gD were confirmed by SDS-PAGE and Western blot analysis, respectively. Optimal infection dose and time were also determined for the production of each recombinant PRV glycoprotein. Confirmation of glycosylation of recombinant gB, gC and gD suggested their usefulness as antigens for the development of diagnosis kit or vaccines for Aujeszky's disease.

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