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Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea  

Wang Yong (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Choi Jae Young (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Kang Joong Nam (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Choi Heekyu (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Roh Jong Yul (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Li Ming Shun (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University)
Im Dae Joon (National Institute of Agricultural Science and Technology, Rural Development Administration)
Je Yeon Ho (School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.11, no.2, 2005 , pp. 87-91 More about this Journal
Abstract
We have first isolated and characterized a Plutella xylostella granulovirus ($P_xGV$) from dead larvae of P. xylostella in Korea. The granule of $P_xGV$ was ovoidal shape with an approximate measure of $350-400nm{\times}150-200nm$, and each granule contained one single rod-shape virion with a mean size of $150-180nm{\times}20-30nm$. The major granule protein, granulin, had a molecular weight of approximately 29kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported $P_xGV$, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported $P_xGV$. These results suggested that the $P_xGV$ isolated in this study was a novel isolate containing different genomic information.
Keywords
Bculovirus; Plutella xylostella granulovirus; Granulin gene; p10 gene;
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