• Research in Plant Disease
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• v.18 no.1
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• pp.45-48
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• 2012

In early August of 2011, following a prolonged period of cool and moist weather, several trees of boxelder maple (Acer negundo) planted in Mt. Soyo located in Dongducheon, Korea, were found to be heavily damaged by premature defoliation with zonate leaf spot symptoms. Numerous number of cone-shaped, white sporophores (conidia) were observed on lesions of the abaxial leaf surface. The morphological characteristics of conidia are of typical Cristulariella moricola, which was supported by analyzing sequences of internal transcribed spacer region of ribosomal DNA. Pathogenicity of the fungus was proved by artificial inoculation in the condition of relative humidity 100% and $18{\pm}2^{\circ}C$. This is the first report of the occurrence of zonate leaf spot caused by infection of C. moricola on A. negundo in Korea.

• Journal of Life Science
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• v.22 no.3
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• pp.285-292
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• 2012

Genus Spiraea is composed of many long-lived woody species that are primarily distributed throughout Asia and Europe. In this study, we evaluated a representative sample of the 38 taxa in the world, including 14 in Korea, with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. The molecular data allowed us to resolve well-supported clades in the taxa. In 47 world accessions (38 taxa: 14 Korean taxa, 33 world taxa, and 9 overlapping taxa), total alignment length was 689 positions, of which 452 were parsimony informative, 527 variable, 75 singleton, and 159 constant characters. Although the phylogenic tree showed that many taxa of genus Spiraea were well separated from each other, many branches were not congruent with the morphological characteristics and geographical distributions of the genus. There were 430 segregating sites and the nucleotide diversity (${\pi}$) value was 0.281. Under the neutral mutation hypothesis, the probability that the Tajima test statistic (D) is positive (2.325) is more than 0.5. Therefore, there may be a site at which natural selection, which increases genetic variation, is operating.

• Research in Plant Disease
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• v.27 no.2
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• pp.66-69
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• 2021

In 2020, a brown felt was observed on persimmon (Diospyros kaki) in persimmon orchards, Sangju, Korea. The symptom on persimmon was white to grey mycelial mats on some areas of the branches. Each mat progressively expanded until the mats coalesced to occupy larger areas and finally girdled the branches. The disease branches were covered with brown-colored mold, consisting of hyphal mats of the pathogen. Optimum temperature for mycelial growth was 30℃. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete internal transcribed spacer rDNA region, the causal fungus was identified as Septobasidium sp. This is the first report of brown felt caused by Septobasidium sp. on persimmon in Korea.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.26-33
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• 2021

Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.

• The Korean Journal of Mycology
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• v.51 no.1
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• pp.51-56
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• 2023

Dendropanax trifidus belonging to the family Araliaceae, is a warm-temperate evergreen tree distributed in Jeju Island, Bogil Island, Geomun Island, Geoje Island, Wando, and Haenam in Korea. In June 2021, a root rot disease in which branches of Dendropanax trifidus seedlings turned brown and shrunk was discovered at the seedling cultivation facility in Naju-si, Republic of Korea. To identify the root rot fungus, three strains were isolated from the diseased tissues of seedlings and their mycological characteristics were investigated on potato dextrose agar. In addition, a molecular phylogenetic analysis was performed using sequences of the internal transcribed spacer (ITS) region and translation elongation factor 1-α (EF1-α) gene. The fungus was identified as Fusarium oxysporum. For pathogenicity test, the roots of seedlings were immersed in the conidia suspension of the strains and planted. After 20 days inoculation, root rot and browning symptoms were confirmed in the inoculated plants. This is the first report of F. oxysporum on D. trifidus in Korea.

• Journal of the Korean Applied Science and Technology
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• v.34 no.1
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• pp.33-40
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• 2017

To isolate and identify the yeast strains associated with D. morbifera, homogenized D. morbifera root samples were spread onto GPY, DG18, SCG and DOB agar media containing antibiotics, Triton X-100, and l-sorbose. Total 81 yeast isolates were analyzed by sequencing of internal transcribed spacer (ITS) region of the ribosomal DNA. The results showed that the root-associated yeast species were composed of the genera Vanderwaltozyma (40 isolates), Cryptococcus (40 isolates), and Kluyveromyces (one isolate). Moreover, the Kluyveromyces isolate exhibited high bioethanol productivity. In addition, the Vanderwaltozyma and Cryptococcus were dominant in D. morbifera roots. The specific yeast community associated with D. morbifera roots was identified by phylogenetic sequence analyses. These yeast isolates may have industrial applications as biosurfactant and bioethanol.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.45-45
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• 2014

Individualities of precious health mushroom called Zhenghonggu@ from respective protections scattered among all main mountains of Fujian China were collected and recognized locally, then compared with Russula griseocarnosa. Their internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear rDNA were amplified, AMOVA analyzed, nested clade analyzed and then compared with the ITS sequences of relative Russula species from other regions of China to confirm the taxonomic status of Zhenghonggu$^@$ and its population structure. Total 23 haplotypes from different protections of Fujian can be clustered into three clades similar to the three lineages of Dahongjun$^@$ from southeastern China reported by Li et al. The geographic distribution characteristic of these three phylogeny clades may be closely coupled with the vegetation regionalization and/or the differences of coenosium construction of Fagaceae that is the host of Russula griseocarnosa. The correlation of taxonomy, phylogeny and geographical distribution of Russula are discussed.

• Mycobiology
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• v.48 no.4
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• pp.296-303
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• 2020

Three yeast strains (Hue-1, Hue-8, and Hue-19) with strong heavy metal tolerance were isolated from mangosteen from Hue city, Vietnam. They exhibited identical phenotype and phylogeny. Sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region demonstrated that the closest relative of these strains is Papiliotrema sp. with 2.12% and 3.55-3.7% divergence in the D1/D2 domain, and ITS domain, respectively. Based on the physiological, biochemical, and molecular data, the three strains belong to a novel species of Papiliotrema genus, for which the name Papiliotrema huenov sp. nov. is proposed. These strains are highly tolerant of heavy metals compared to other yeasts, being able to grow in the presence of 2 mM Pb (II), 2 mM Cd (II), and up to 5 mM Ni (II), but no growth was observed in the presence of 1 mM As (III).

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.63-67
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• 2017

Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

• Animal Systematics, Evolution and Diversity
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• v.34 no.4
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• pp.194-198
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• 2018

The genus Prionchulus Cobb, 1916 represents a group of predaceous nematodes belonging to the family Mononchidae Chitwood, 1937, and is found worldwide. However, only five species have been reported thus far from Korea. Prionchulus oleksandri Winiszewska and Susulovsky, 2003 is reported for the first time from Korea, from sediments collected from the Nakdong River. This species is distinguished from other Prionchulus species by its truncated lip region with small cephalic papillae and refringens vaginae. In this study, morphological characters(detailed morphometrics) of P. oleksandri are described and illustrated using optical microscopy. DNA barcode sequence information (the D2-D3 region of 28S rDNA, 18S rDNA, and internal transcribed spacer rDNA) is also provided for the molecular identification of the species.