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Brown Felt on Persimmon (Diospyros kaki) Caused by Septobasidium sp. in Korea

Septobasidium sp.에 의한 감 갈색고약병 발생

  • Ha, Jeong-Seok (Gyeongsangbuk-do Agricultural Research and Extention Services Sangju Persimmon Research Institute) ;
  • Song, In-Kyu (Gyeongsangbuk-do Agricultural Research and Extention Services Sangju Persimmon Research Institute) ;
  • Kim, Seung-Han (Gyeongsangbuk-do Agricultural Research and Extention Services) ;
  • Kim, Ji-Won (Gyeongsangbuk-do Agricultural Research and Extention Services)
  • 하정석 (경상북도농업기술원 상주감연구소) ;
  • 송인규 (경상북도농업기술원 상주감연구소) ;
  • 김승한 (경상북도농업기술원) ;
  • 김지원 (경상북도농업기술원)
  • Received : 2021.02.08
  • Accepted : 2021.04.19
  • Published : 2021.06.30

Abstract

In 2020, a brown felt was observed on persimmon (Diospyros kaki) in persimmon orchards, Sangju, Korea. The symptom on persimmon was white to grey mycelial mats on some areas of the branches. Each mat progressively expanded until the mats coalesced to occupy larger areas and finally girdled the branches. The disease branches were covered with brown-colored mold, consisting of hyphal mats of the pathogen. Optimum temperature for mycelial growth was 30℃. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete internal transcribed spacer rDNA region, the causal fungus was identified as Septobasidium sp. This is the first report of brown felt caused by Septobasidium sp. on persimmon in Korea.

2020년 경상북도 상주시 감 과수원에서 갈색고약병이 발생하였다. 병징은 처음에 자갈색 내지 암갈색의 균사체가 가지 표면 일부분에 들러붙어 있다가 점차 균사체들이 자라면서 큰 덩어리로 합쳐져 결국엔 가지를 완전히 둘러싸였다. 균총의 색깔은 처음에 흰색이고 배양기간이 경과됨에 따라 배지 표면에 갈색의 균사가 많이 형성되었다. 균사생육 적온은 30℃이었다. rDNA의 complete internal transcribed spacer 영역의 염기서열을 분석하였고, 분석된 염기서열(564 bp)을 BLASTN 프로그램으로 확인한 결과, Septobasidium속(GenBank accession nos. HQ267951, HQ267959)과 99%의 상동성을 나타내었다. 또한, Septobasidium broussonetiae (HM209416)와 92%, Septobasidium bogoriense (HM209414)와 91%의 상동성을 나타내었다. 이와 같이 감에서 발생한 병징과 병원균의 균학적 특징을 기초로 하여 이 병을 Septobasidium속에 의한 감 갈색고약병으로 명명하고자 제안한다.

Keywords

References

  1. Choi, I.-Y., Lee, W.-H., Lee, J.-I., Park, M.-J., Ko, J.-A., Choi, J.-R. et al. 2016. Charaterization of a Septobasidium sp. associated with felt disease of Schisandra chinensis. Mycobioloby 44: 58-62. https://doi.org/10.5941/MYCO.2016.44.1.58
  2. Gomez, L. D. and Henk, D. A. 2004. Validation of the species of Septobasidium (Basidiomycetes) described by John N. Couch. Lankesteiana 4: 75-96.
  3. Tamura, K., Dudley, J., Nei, M. and Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599. https://doi.org/10.1093/molbev/msm092
  4. The Korean Society of Plant Pathology. 2020. List of plant diseases in Korea. URL http://genebank.rda.go.kr/plntDissInfoMain.do [8 February 2021].
  5. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.