• The Korean Journal of Physiology and Pharmacology
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• v.21 no.2
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• pp.233-239
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• 2017

Intracellular calcium ($Ca^{2+}$) oscillation is an initial event in digestive enzyme secretion of pancreatic acinar cells. Reactive oxygen species are known to be associated with a variety of oxidative stress-induced cellular disorders including pancreatitis. In this study, we investigated the effect of hydrogen peroxide ($H_2O_2$) on intracellular $Ca^{2+}$ accumulation in mouse pancreatic acinar cells. Perfusion of $H_2O_2$ at $300{\mu}M$ resulted in additional elevation of intracellular $Ca^{2+}$ levels and termination of oscillatory $Ca^{2+}$ signals induced by carbamylcholine (CCh) in the presence of normal extracellular $Ca^{2+}$. Antioxidants, catalase or DTT, completely prevented $H_2O_2$-induced additional $Ca^{2+}$ increase and termination of $Ca^{2+}$ oscillation. In $Ca^{2+}$-free medium, $H_2O_2$ still enhanced CCh-induced intracellular $Ca^{2+}$ levels and thapsigargin (TG) mimicked $H_2O_2$-induced cytosolic $Ca^{2+}$ increase. Furthermore, $H_2O_2$-induced elevation of intracellular $Ca^{2+}$ levels was abolished under sarco/endoplasmic reticulum $Ca^{2+}$ ATPase-inactivated condition by TG pretreatment with CCh. $H_2O_2$ at $300{\mu}M$ failed to affect store-operated $Ca^{2+}$ entry or $Ca^{2+}$ extrusion through plasma membrane. Additionally, ruthenium red, a mitochondrial $Ca^{2+}$ uniporter blocker, failed to attenuate $H_2O_2$-induced intracellular $Ca^{2+}$ elevation. These results provide evidence that excessive generation of $H_2O_2$ in pathological conditions could accumulate intracellular $Ca^{2+}$ by attenuating refilling of internal $Ca^{2+}$ stores rather than by inhibiting $Ca^{2+}$ extrusion to extracellular fluid or enhancing $Ca^{2+}$ mobilization from extracellular medium in mouse pancreatic acinar cells.

• Journal of Bio-Environment Control
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• v.27 no.2
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• pp.147-157
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• 2018

With the rapid development of the 4th industrial revolution, the large-sized facilities of agriculture have been developed with high-technologies. However, it is difficult to maintain the optimum environment in large-sized facilities. Although it is essential to control micro-climate properly in large-sized facilities, there are a lot of problems to utilize high-technologies and equipment because of insufficient education for farmers. Most farms have limitations to access to their farm because of prevention of epidemics, exposure of management know-how, and so on. Especially, it is difficult to understand internal environmental factors (airflow, temperature, humidity, etc.) for farmers because these factors are invisible. Recently, Virtual reality technology which allows users to experience various phenomena directly is attracting attention. Virtual reality is very useful technology to visualize airflow and temperature distribution and so on. However, there is no cases applied this technology to agricultural facilities. In this study, research trends of virtual reality in various fields were investigated. In particular, the limitation and possibility of virtual reality technology were analyzed for educating farmers. Finally, the development of virtual reality contents for smart-farm facility were suggested.

• Tuberculosis and Respiratory Diseases
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• v.79 no.4
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• pp.257-266
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• 2016

Background: Idiopathic pulmonary fibrosis is a common interstitial lung disease; it is a chronic, progressive, and fatal lung disease of unknown etiology. Over the last two decades, knowledge about the underlying mechanisms of pulmonary fibrosis has improved markedly and facilitated the identification of potential targets for novel therapies. However, despite the large number of antifibrotic drugs being described in experimental pre-clinical studies, the translation of these findings into clinical practices has not been accomplished yet. NADH:quinone oxidoreductase 1 (NQO1) is a homodimeric enzyme that catalyzes the oxidation of NADH to $NAD^+$ by various quinones and thereby elevates the intracellular $NAD^+$ levels. In this study, we examined the effect of increase in cellular $NAD^+$ levels on bleomycin-induced lung fibrosis in mice. Methods: C57BL/6 mice were treated with intratracheal instillation of bleomycin. The mice were orally administered with ${\beta}$-lapachone from 3 days before exposure to bleomycin to 1-3 weeks after exposure to bleomycin. Bronchoalveolar lavage fluid (BALF) was collected for analyzing the infiltration of immune cells. In vitro, A549 cells were treated with transforming growth factor ${\beta}1$ (TGF-${\beta}1$) and ${\beta}$-lapachone to analyze the extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT). Results: ${\beta}$-Lapachone strongly attenuated bleomycin-induced lung inflammation and fibrosis, characterized by histological staining, infiltrated immune cells in BALF, inflammatory cytokines, fibrotic score, and TGF-${\beta}1$, ${\alpha}$-smooth muscle actin accumulation. In addition, ${\beta}$-lapachone showed a protective role in TGF-${\beta}1$-induced ECM expression and EMT in A549 cells. Conclusion: Our results suggest that ${\beta}$-lapachone can protect against bleomycin-induced lung inflammation and fibrosis in mice and TGF-${\beta}1$-induced EMT in vitro, by elevating the $NAD^+$/NADH ratio through NQO1 activation.

• Clinics in Shoulder and Elbow
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• v.23 no.1
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• pp.3-10
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• 2020

Background: The aim of this study was to evaluate clinical experience with arthroscopic debridement for septic arthritis of the shoulder joint and to report on our patient outcomes. Methods: The retrospective analysis included 36 shoulders (male:female, 15:21), contributed by 35 patients (mean age, 63.8 years) treated by arthroscopy for septic arthritis of the shoulder between November 2003 and February 2016. The mean follow-up period was 14.3 months (range, 12-33 months). An additional posterolateral portal and a 70° arthroscope was used to access the posteroinferior glenohumeral (GH) joint and posteroinferior subacromial (SA) space, respectively. Irrigation was performed with a large volume of fluid (25.1±8.1 L). Multiple suction drains (average, 3.3 drains) were inserted into the GH joint and SA space and removed 8.9±4.3 days after surgery. Intravenous antibiotics were administered for 3.9±1.8 weeks after surgery, followed by oral antibiotic treatment for another 3.6±1.9 weeks. Results: Among the 36 shoulders, reoperation was required in two cases (5.6%). The average range of motion achieved was 150.0° for forward flexion and T9 for internal rotation. The mean simple shoulder test score was 7.9±3.6 points. Nineteen shoulders (52.8%) had acupuncture or injection history prior to the infection. Pathogens were identified in 15 shoulders, with Staphylococcus aureus being the most commonly identified pathogen (10/15). Both the GH joint and the SA space were involved in 21 shoulders, while 14 cases involved only the GH joint and one case involved only the SA space. Conclusions: Complete debridement using an additional posterolateral portal and 70° arthroscope, a large volume of irrigation with >20 L of saline, and multiple suction drains may reduce the reoperation rate.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.740-746
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• 2017

Infrared signatures emitted from hot exhaust gases generated by the internal combustion engine and generator of naval ships and from the metal surfaces of the funnel have become the targets of infrared homing missiles, which is the main cause of a reduced survivability of naval ships. The infrared signatures from the exhaust gas and the metal surface of a funnel can be reduced by installing an infrared signature suppression (IRSS) system on a ship. The IRSS system consists of three parts: an eductor that generates turbulent flow of the exhaust gas, a mixing tube that mixes the exhaust gas with ambient air, and a diffuser that forms an air film using the pressure difference between the inside and outside air. As a basic study to develop an IRSS system using domestic technology, this study analyzed the model test conditions of an IRSS system developed by an overseas engineering company and installed on a domestic naval ship, and a numerical heat-flow analysis was conducted based on the results of the aforementioned analysis. Numerical heat-flow analysis was performed using a commercial numerical-analysis application, and various turbulence models were considered. As a result, the temperature and velocity of the exhaust gas at the educator inlet and diffuser outlet and that of the metal surface of the diffuser were measured, and found to agree well with the measurement results of the model test.

• Journal of Korean Neurosurgical Society
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• v.29 no.6
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• pp.786-793
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• 2000

Objectives : A clinical analysis was performed to provide management strategy and to improve management outcome of elderly patients with intracranial aneurysm. Patients and Methods : We reviewed medical records of 746 consecutive patients with intracranial aneurysm who were admitted from July 1991 to December 1996. They were divided into two age groups : elderly(120 patients aged 65 years or older) and non-elderly(626 patients aged 64 years or younger). We investigated the differences between the two groups in clinical characteristics, management outcome and surgical results. Results : Female(80.0%), internal carotid artery aneurysm(48.9%), poor clinical grade(Hunt and Hess Grade IV, V : 39.8%), postoperative subdural fluid collection(38.2%), and postoperative hydrocephalus(39.7%) were more frequent in the elderly patients. There were no significant differences in the incidence of hypertension, multiple aneurysm, unruptured aneurysm, rebleeding, delayed ischemic neurological deficits, postoperative hemorrhage, and low density on the postoperative brain CT scan. In some cases, surgical clipping of ruptured aneurysm could not be performed due to moribund state or refusal of surgery by the elderly patient's family. Both management outcome and surgical results in elderly aneurysm patients at 3 months after rupture were worse than those of the non-elderly group. The most common reason of unfavorable outcome was poor clinical grade in both groups, while serious medical illness causing unfavorable outcome was more common in the elderly group. Conclusion : Surgical treatment of a ruptured aneurysm should not be avoided in elderly patient solely on the basis of advanced age. If the patients are in good clinical grade, early aneurysm surgery followed by early ambulation should be recommended. Further improvements in outcome may be achieved by thorough knowledge of poor resilience of brain, CSF flow dynamics, and diminished cardiopulmonary reserve in elderly patients with intracranial aneurysm.

• Clean Technology
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• v.26 no.1
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• pp.1-6
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• 2020

Rapid expansion of supercritical solution (RESS) process was used to make molsidomine (MOL) loaded peracetyl-β-cyclodextrin (PAc-β-CD) nanoparticles, which were collected into the air. The effect of the concentration of the drug PAc-β-CD (0.5 and 1 wt%), extraction temperature (45 ~ 60 ℃), nozzle length (5 ~ 20 mm) and internal diameter (ID) (50 ~ 150 μm) of a capillary, and spray distance on the particle size and morphology of the resulting particles were investigated. The interaction of a drug and PAc-β-CD was confirmed by ¹H-NMR spectroscopy while the particle size was measured by means of a scanning electron microscope. It was found that increasing the temperature from 45 ℃ to 60 ℃ and decreasing the nozzle diameter from 150 μm to 50 μm had an increasing effect on the average particle size, while increasing the spray distance led to a decrease in the average particle size at a constant pressure of 34.5 MPa and temperature of 45 ℃. With 0.5 wt% of PAc-β-CD, the capillary nozzle of short length (5 mm) and small ID (50 μm) gave the smallest size (165 nm). The obtained nanoparticles showed increased dispersity and solubility in oil. The oil suspension of the inclusion complex showed increased sustainability, which can increase the in-vitro controlled release time of the drug.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.83-89
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• 2008

A wind tunnel test was conducted at Protected Horticulture Experiment Station of National Horticultural Research Institute in Busan to find the aerodynamic resistance and quadratic resistance coefficient of chrysanthemum in greenhouse. The internal plants of the CFD model has been designed as a porous media because of the complexity of its physical shapes. Then the aerodynamic resistance value should be input for analyzing CFD model that crop is considered while the value varies by crops. In this study, the aerodynamic resistance value of chrysanthemum canopy was preliminarily found through wind tunnel test. The static pressure at windward increased as wind velocity and planting density increased. The static pressure at leeward decreased as wind velocity increased but was not significantly affected by planting density. The difference of static pressure between windward and leeward increased as wind velocity and planting density increased. The aerodynamic resistance value of chrysanthemum canopy was found to be 0.22 which will be used later as the input data of Fluent CFD model. When the planting distances were $9{\times}9\;cm$, $11{\times}11\;cm$, and $13{\times}13\;cm$, the quadratic resistance coefficients of porous media were found to be 2.22, 1.81, and 1.07, respectively. These values will be used later as the input data of CFX CFD model.

• Journal of Oriental Neuropsychiatry
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• v.17 no.2
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• pp.61-73
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• 2006

Objective : This study was to investigate the effects of Banhabaekchulchunma-Tang(BCT) on Dementia induced by focal ischemic injury in the rats. Method : Experimental groups were divided into 4 groups ; Normal group, Control group, BCT1 group and BCT2 group. Control group were no treated after focal brain ischemic injury. BCT1 group were administered BCT 0.3 ml/kg to focal brain ischemic injuried rats for 21 days, BCT2 group were administered BCT 1.2 ml/kg to focal brain ischemic injuried rats for 21 days. The present author observed the number of errors on the eight-arm radial maze task , the rate of correct choice on the eight-arm radial maze task, the values of density of Cresyl Violet-stained sections in the hippocampal CA1 and the values of density of Acetlycholin Esterase(AchE) stained nuclei in the hippocampal CA1. Result : 1. The number of errors in the eight-arm radial maze task was significantly decreased in BCT1 group on 1-6th days. And it was significantly decreased in BCT2 group on 1st, 2nd, 3rd and 6th day compared with control group. 2. The rate of correct choice on the eight-arm radial maze task was increased in BCT1 group and BCT2 group compared with control group, but the value was not significant. 3. The values of density of Cresyl Violet-stained sections in the hippocampal CA1 were significantly increased in BCT1 group and BCT2 group compared with control group. 4. The values of density of AchE stained nuclei in the hippocampal CA1 were significantly increased in BCT1 group compared with control group. Conclusion : The present author thought that Banhabaekchulchunma-Tang could be used to cure dementia derived by the phlegm retention of fluid.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.427-431
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• 2018

We report a case of a patient with necrotizing fasciitis followed by liver abscess. A 51-year-old man was admitted to our hospital with a 5-day history of fever and chills with painful swelling of the right thigh. A magnetic resonance imaging (MRI) scan showed fluid collection with numerous dark signal intensities considered as air-bubbles between the posteromedial and posterolateral groups of the right thigh, resulting in presumptive diagnosis of necrotizing fasciitis. At the time of admission, an ultrasonograph of the abdomen showed increased parenchymal echogenicity of both kidneys and no liver abscess. Ten days after fasciotomy, an abdominal computed tomography (CT) scan showed intrahepatic abscess. Sonography-guided percutaneous drainage was performed. Both cultures of pus specimens from the liver abscess and right thigh yielded Klebsiella pneumoniae (K. pneumoniae). The patient was treated with fasciotomy several times and parenteral antibiotics, after which he began to improve. After 5 weeks, liver abscess size was reduced, and after 10 weeks, liver abscess disappeared. To the best of our knowledge, this is the first case of K. pneumoniae-necrotizing fasciitis followed by liver abscess.