• 한국임상수의학회지
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• 제28권4호
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• pp.427-430
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• 2011

6개월령의 잡종 수컷 고양이가 갑작스런 호흡곤란과 식욕부진 증상으로 내원 하였다. 신체검사상 심음의 감소와 함께 확연한 호흡곤란이 확인 되었다. 혈액학적 검사에서 고글로블린 혈증을 동반한 고단백혈증이 관찰되었으며, 다클론성 감마글로블린병증이 확인 되었다. 환축은 흉부 방사선 검사에서 흉수가 확인 되었으며, 흉수는 비감염성 삼출물로, 흉수 역시 다클론성 감마글로블린병증을 동반한 고글로블린증과 감소한 알브민 대 글로블린 비율이 관찰되었다. 또한 흉강 삼출물을 통한 역전사 중합효소연쇄반응에서 고양이 코로나바이러스에 양성반응을 확인하였다. 따라서, 환축은 고양이 전염성 복막염이 강하게 의심되었으며, 재조합 인간 인터페론 알파, pentoxifylline 과 스테로이드제를 이용한 적극적인 치료가 시작 되었다. 환축은 초기 치료에 반응이 좋았으며, 치료 이후 5개월간 재발 증상이 관찰되지 않았다. 결론적으로 본 증례는 재조합 인간 인터페론 알파와 pentoxifylline을 이용한 고양이 전염성 복막염의 치료 반응에 대한 국내 첫 증례보고이다.

• Tuberculosis and Respiratory Diseases
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• 제54권3호
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• pp.346-352
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• 2003

저자들은 호흡곤란, 객담, 기침을 주소로 내원한 23세 남자환자에서 기관지 내시경 및 VATS로 시행한 조직검사상 후두와 함께 기관, 기관지 및 폐실질을 침범한 유두종증으로 진단받고 interferon-${\alpha}$ 치료 후 호전을 보인 재발성 호흡기계 유두종증 1예를 경험하였기에 보고하는 바이다.

• 대한수의학회지
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• 제40권1호
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• pp.166-172
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• 2000

Prior to a clinical trial, the in vitro and in vivo antitumor effects of a new recombinant human interferon ${\alpha}-2a$ (rHu/IFN ${\alpha}-2a$) with/without hydroxyurea (HU) were investigated using chronic myelogenous leukemia (CML)-derived cell lines (K562 and KU812F) and BALB/c nude. mice transplanted with KU812F cells. The rHu/IFN ${\alpha}-2a$ ($10^4-10^6IU/ml$) strongly inhibited proliferation of both cell lines and the combined treatments with HU ($10{\mu}g/ml$) were more effective. In nude mice transplanted with KU812F cells. rHu/IFN ${\alpha}-2a(1{\times}10^6IU$) inhibited tumor growth by 42-65% at 15-21 days post-transplantation (DPT). The combined treatment of rHu/IFN ${\alpha}-2a (5{\times}10^5IU$) with HU (0.25mg/g b.w.) inhibited the tumor growth by 48-67% at 12-21 DPT. In addition, the treatment of rHu/IFN ${\alpha}-2a$ ($5{\times}10^6IU\;or\;1{\times}10^7IU$) rejected tumor transplantation by 40%. These results suggest that the new rHU/IFN ${\alpha}-2a$ alone or with HU is effective on CML cell lines.

• 한국미생물·생명공학회지
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• 제35권3호
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• pp.226-230
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• 2007

산소를 많이 소비하는 발효공정일수록 배양액중의 용존산소의 농도가 목적생산물의 생산성에 많은 영향을 주는 경우가 많다. 때문에 고농도 발효에 앞서, 발효조의 sparging hole로부터 임펠러 높이에 따른 산소전달 능력을 알아본 결과 공기공급이 1 vvm, 교반속도가 600 rpm에서 산소전달계수($K_La$)는 2.67($min^{-1}$)으로 가장 높았다. 배양 시 용존산소 농도를 20% 이상 유지시켰을 때 온도에 따른 k6ub/IFN-${\alpha}1$ 생성은 $30^{\circ}C$에서 세포증식을 하고 $25^{\circ}C$에서 IPTG로 Induction 하였을 때 발현율이 6.43mg/ml로 total protein의 37%로 가장 많은 양이 발현되는 것을 알 수 있었다. 용존산소 농도에 따른 k6ub/IFN-${\alpha}1$의 발현양은 용존산소 농도가 35%일 때 가장 높은 수율을 나타냈다. 용존산소량은 산소소비 속도를 측정함으로써 정확한 임계점을 찾을 수 있었는데 용존산소량이 35% 유지될 때 산소 전달 속도와 비교하여 가장 적당한 산소공급량임을 확인할 수 있었다.

• 대한한의학회지
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• 제17권2호
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• pp.227-236
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• 1996

The effect of Ligustrum Lucidum(LL) on the production of nitric oxide (NO) and superoxide by murine peritoneal macrophages were investigated. Stimulation of the cells with LL in the presence or absence of interferon-r(IFN-r) resulted in the increased accumulation of nitrite in the medium. To further examine the mechanism of LL induced. NO Synthesis, we evaluated the secretion of tumor necrosis $factor-{\alpha}(TNF-{\alpha})$ by LL in murine macrophages. Treatment of LL increased the secretion of bioactive $TNF-{\alpha}$ in cultured medium. In addition, LL induced NO production was decreased by the treatment of anti-murine $TNF-{\alpha}$. neutralizing antibodies, indicating that LL induced superoxide production was decreased by the treatment of anti-murine $TNF-{\alpha}$ neutralizing antibodies. These data suggested that LL induced superoxide production was related to $TNF-{\alpha}$ secretion. In conclusion, our results indicates that LL may enhance innate immune response and be applied as a immunoregulating drug improving phagocytosis.

• 대한한방부인과학회지
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• 제21권1호
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• pp.39-54
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• 2008

Purpose: The purpose of this study was to investigate the anti-inflammatory effects of the water extract of Omisodokeum (OMSDE) on peritoneal macrophages, Methods: To verify the anti-inflammatory mechanism of OMSDE, the activation of nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ and the phosphorylation of MAPK were examined. Results: The extract of OMSDE suppressed the production of LPS-induced nitric oxide (NO), tumor necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$, IL-6 and IL-12 in the macrophages. OMSDE inhibited the degradation of inhibitory ${\kappa}B-{\alpha}$ $(I{\kappa}B-{\alpha})$ and it suppressed the activation of extracellular signal-regulated kinase (ERK 1/2) but didn't inhibit c-Jun N-terminal kinase (JNK) and p38, indicating that OMSDE may inhibit the pro-inflammatory cytokine production process by inhibiting the activation of $NF-{\kappa}B$ and ERK 1/2. Furthermore, OMSDE inhibited the production of interferon $(IFN)-{\beta}$ but didn't inhibit of $IFN-{\alpha}$ in the LPS-stimulated macrophages through the down-regulation of interferon regulatory factor (IRF)-1 and IRF-7. The Oral administration of OMSDE inhibited LPS-induced endotoxin shock and the production of $TNF-{\alpha}$ in serum but didn't inhibit of $IL-1{\beta}$ and IL-6. Conclusion: These results suggest that OMSDE may be effective in the prevention and treatment of inflammatory diseases.

• Journal of Animal Science and Technology
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• 제65권1호
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• pp.183-196
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• 2023

Interferon-alpha inducible protein 6 (IFI6) is an interferon-stimulated gene (ISG), belonging to the FAM14 family of proteins and is localized in the mitochondrial membrane, where it plays a role in apoptosis. Transcriptional regulation of this gene is poorly understood in the context of inflammation by intracellular nucleic acid-sensing receptors and pathological conditions caused by viral infection. In this study, chicken IFI6 (chIFI6) was identified and studied for its molecular features and transcriptional regulation in chicken cells and tissues, i.e., lungs, spleens, and tracheas from highly pathogenic avian influenza virus (HPAIV)-infected chickens. The chIFI6-coding sequences contained 1638 nucleotides encoding 107 amino acids in three exons, whereas the duck IFI6-coding sequences contained 495 nucleotides encoding 107 amino acids. IFI6 proteins from chickens, ducks, and quail contain an IF6/IF27-like superfamily domain. Expression of chIFI6 was higher in HPAIV-infected White Leghorn chicken lungs, spleens, and tracheas than in mock-infected controls. TLR3 signals regulate the transcription of chIFI6 in chicken DF-1 cells via the NF-κB and JNK signaling pathways, indicating that multiple signaling pathways differentially contribute to the transcription of chIFI6. Further research is needed to unravel the molecular mechanisms underlying IFI6 transcription, as well as the involvement of chIFI6 in the pathogenesis of HPAIV in chickens.

• 한국미생물·생명공학회지
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• 제36권4호
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• pp.353-359
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• 2008

Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.

• BMB Reports
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• 제52권5호
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• pp.336-341
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• 2019

The cGAS-STING pathway plays an important role in pathogen-induced activation of the innate immune response. The 29-kDa amino-terminal fibronectin fragment (29-kDa FN-f) found predominantly in the synovial fluid of osteoarthritis (OA) patients increases the expression of catabolic factors via the toll-like receptor-2 (TLR-2) signaling pathway. In this study, we investigated whether 29-kDa FN-f induces inflammatory responses via the cyclic GMP-AMP synthase (cGAS)/stimulator of interferon gene (STING) pathway in human primary chondrocytes. The levels of cGAS and STING were elevated in OA cartilage compared with normal cartilage. Long-term treatment of chondrocytes with 29-kDa FN-f activated the cGAS/STING pathway together with the increased level of gamma-H2AX, a marker of DNA breaks. In addition, the expression of pro-inflammatory cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF/CSF-2), granulocyte colony-stimulating factor (G-CSF/CSF-3), and type I interferon ($IFN-{\alpha}$), was increased more than 100-fold in 29-kDa FN-f-treated chondrocytes. However, knockdown of cGAS and STING suppressed 29-kDa FN-f-induced expression of GM-CSF, G-CSF, and $IFN-{\alpha}$ together with the decreased activation of TANK-binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3), and inhibitor protein ${\kappa}B{\alpha}$ ($I{\kappa}B{\alpha}$). Furthermore, NOD2 or TLR-2 knockdown suppressed the expression of GM-CSF, G-CSF, and $IFN-{\alpha}$ as well as decreased the activation of the cGAS/STING pathway in 29-kDa FN-f-treated chondrocytes. These data demonstrate that the cGAS/STING/TBK1/IRF3 pathway plays a critical role in 29-kDa FN-f-induced expression of pro-inflammatory cytokines.

• 농업과학연구
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• 제12권1호
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• pp.153-161
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• 1985

고단위(高單位)의 porcine leucocyte interferon(For IFN-${\alpha}$)을 대량생산(大量生産)하기 위한 기초연구(基礎硏究)로써 백혈구(白血球) 분리법(分離法), 백혈구(白血球)의 성상(性狀) 및 inducer에 따른 IFN 생산효과(生産效果) 시험(試驗)을 하였고 Por IFN-${\alpha}$의 역가측정(力價測定)을 micro-system에 확립(確立)하기 위해 시험(試驗)을 수행(遂行)하였던 바 다음과 같은 결과(結果)를 얻었다. 1. 돼지 말초백혈구(末梢稍白血球)를 분리(分離)하기 위해서 4 가지 방법(方法)을 응용(應用)하던 바 백혈구(白血球) 분리법(分離法)은 buffy coat 方法에서 가장 높았으나 적혈구(赤血球) 혼입율(混入率)이 높았고 Ficoll-paque 방법(方法)에서는 백혈구(白血球) 분리율(分離率)은 중등도(中等度)였으나 적혈구(赤血球) 혼입율(混入率)은 가장 낮았다. 2. 말초백혈구(末梢白血球)와 비장세포(脾臟細胞)의 활성도(活性度)는 IFN 생산(生産)에 영향(影響)을 주었으며 세포활성도(細胞活性度)가 높을수록 IFN 생산효능(生産效能)이 높았으며, 적혈구(赤血球)의 혼입도(混入度)가 높을수록 IFN 생산효능(生産效能)은 낮았다. 말초백혈구(末梢白血球)는 비장세포(脾臟細胞)에 비해 1.7배(倍)의 높은 IFN 생산효과(生産效果)가 인정(認定)되었다. 3. Inducer로 사용(使用)된 Sendai virus(Cantell 주(株))는 Newcastle disease virus(Lasota 주(株)) 보다 IFN 생산효과(生産效果)가 5.5 배(倍) 높았다. 4. 본(本) 시험(試驗)에서 확립(確立)된 micro-IFN assay 법(法)의 재현성(再現性)을 검사(檢査)한 바 4회반복시험(回反復試驗)에서 편차(偏差)는 0.05~0.21로 나타났다. 5. 시험생산(試驗生産) 9롯트의 IFN 역가(力價)는 330 내지 4,700 unit/ml로써 평균(平均) 1,200 unit/ml이었다.