• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1979.04a
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• pp.113.2-114
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• 1979

$\beta-Galactosidase$ is an enzyme which catalizes hydrolysis of lactose, a natural substrate, to glucose and galctose and transferring some monosac-charide units to active acceptors as sugar or alcohol. The occurence of $\beta-Galactosidase$ is known in various microorganisms, animals and higher plants and has been studied by many investigatigators. Especially, a great deal of articles for the enzyme of E. coli have been presented in genetic control mechanism and induction-repression effects of proteins, On the other hand, in the dairly products industry, it is important to hydrolyes lactosd which is the principal sugar of milk and milk products. During the last few years, the interest in enzymatic hydrolysis of milk lactose has teen increased, because of the lactose intolerence in large groups of the population. Microbial $\beta-Galactosidases$ are considered potentially most suitable for processing milk to hydrolyse lactose and, in recent years, the immobilized enzyme from yeast has been examined. Howev, most of the microbial $\beta-Gal$ actosidase are intracellular enzymes, except a few fungal $\beta-Gala-$ ctosidases, and extracellular $\beta-Galactosidase$ which may be favorable to industrial applieation is not so well investigated. On this studies, a mold producing a potent extracellular $\beta-Galactosidase$ was isolated from soil and identified as an imperfect fungus, Beauveria bassians. In this strain, both extracellular and intracellular $\beta-Galactosidases$ were produced simultaneously and a great increase of the extracellular production was acheved by improving the cultural conditions. The extracellular enzyme was purified more than 1, 000 times by procedures including Phosphocellulose and Sephadex G-200 chromatographies. Several characteristics of the enzymewas clarified with this preparation. The enzyme has a main subunit of molecular weight of 80, 000 which makes an active aggregate. And at neutral pH range, it has optimum pH for activity and stability. The Km value was determined to be 0.45$\times$10$^{-3}$ M for $o-Nitrophenyl-\beta-Galactoside.$ In any event, it is interesting to sttudy the $\beta-Galactosidase$ of B. bassiana for the mechanism of secretion and conformational structure of enzyme.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1216-1221
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• 2014

Poly(ethylene-co-isosorbide terephthalate) (PEIT) is interest in polymer which has isosorbide monomer that is renewable resources such as corn. However, there is important drawback which is low mechnical properties as increasing isosorbide contents. In this study, polycarbonate used to make up for drawback of mechanical properties of PEIT. In addition, PEIT used to improve the tensile elongation of polycarbonate because PEIT has good sheet proccessability. The effect of polycarbonate on morphology, thermal and mechanical properties were investigated using FE-SEM, DMA, TGA, UTM, and notched izod impact strength tester. As a result of this study, PEIT/PC blends were in compatible system and polycarbonate can act as an improvement of thermal stability and mechanical properties in the blends.

• The KIPS Transactions:PartC
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• v.9C no.5
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• pp.687-698
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• 2002

Together with the activation of wireless Internet, the interest for various integrated methods of the existing wired and wireless e-commerces is on the rise. For the integrated wired and wireless e-commerce, firstly, the optimized security and authentication under the radio communication environment should be decided. And also the user-friendly usage is important. In this paper, we designed and implemented wired and wireless interworking Mobile Wallet on terminal that can be used through both wired and wireless methods by developing electronic wallet module for electronic payment using MIDP (Mobile Information Device Profile) based on J2ME (Java 2 Micro Edition). The characteristics of mobile wallet implemented in this paper provide the stability through encoding/decoding payment information and on-line usage on wired/wireless environments through downloading electronic wallet. In addition, we also present safe and convenient payment method for e-commerce based on wired/wireless Internet.

• The Plant Pathology Journal
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• v.36 no.6
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• pp.515-535
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• 2020

Essential oils (EOs) have gained a renewed interest in many disciplines such as plant disease control and medicine. This review discusses the components of ginger EOs, their mode of action, and their potential nanotechnology applications in controlling tropical plant diseases. Gas chromatography-mass spectroscopy (GC-MS), high-performance liquid chromatography, and headspace procedures are commonly used to detect and profile their chemical compositions EOs in ginger. The ginger EOs are composed of monoterpenes (transcaryophyllene, camphene, geranial, eucalyptol, and neral) and sesquiterpene hydrocarbons (α-zingiberene, ar-curcumene, β-bisabolene, and β-sesquiphellandrene). GC-MS analysis of the EOs revealed many compounds but few compounds were revealed using the headspace approach. The EOs have a wide range of activities against many phytopathogens. EOs mode of action affects both the pathogen cell's external envelope and internal structures. The problems associated with solubility and stability of EOs had prompted the use nanotechnology such as nanoemulsions. The use of nanoemulsion to increase efficiency and supply of EOs to control plant diseases control was discussed in this present paper. The findings of this review paper may accelerate the effective use of ginger EOs in controlling tropical plant diseases.

• Journal of Korean Society of Transportation
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• v.27 no.5
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• pp.145-153
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• 2009

Although an interest in higher design speeds continues to increase to promote more efficient travel on expressways, the current Korean design guidelines do not provide criteria for design values. An arising issue associated with higher design speeds is how to effectively ensure traffic safety under such high speed traffic conditions. In particular the safety issue would become more significant in determining the interchange spacing. This study proposes a methodology for determining freeway interchange spacing under higher speed traffic conditions. A microscopic traffic simulator, VISSIM, was used to evaluate the effects of various interchange spacings on traffic conditions in terms of safety. In this study, the acceleration noise was used as an index to represent the stability of traffic conditions, which is a potential indicator to quantify the level of safety. It was found based on simulation evaluations that 5-km interchange spacing would be a feasible alternative under higher speed traffic conditions (around 160 km/h).

• Journal of Platform Technology
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• v.9 no.2
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• pp.46-54
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• 2021

Recently, interest in the safety of Self-driving has been increasing. Self-driving have been studied and developed by many universities, research centers, car companies, and companies of other industries around the world since the middle 1980s. In this study, we propose the automatic extraction method of the threatening obstacle on the Road for the Self-driving. A threatening obstacle is defined in this study as a comparatively large object at center of the image. First of all, an input image and its decreased resolution images are segmented. Segmented areas are classified as the outer or the inner area. The outer area is adjacent to boundaries of the image and the other is not. Each area is merged with its neighbors when adjacent areas are included by a same area in the decreased resolution image. The Obstacle area and Non Obstacle area are selected from the inner area and outer area respectively. Obstacle areas are the representative areas for the obstacle and are selected by using the information about the area size and location. The Obstacle area and Non Obstacle area consist of the threatening obstacle on the road. Through experiments, we expect that the proposed method will be able to reduce accidents and casualties in Self-driving.

• Journal of the Society of Naval Architects of Korea
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• v.56 no.1
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• pp.58-65
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• 2019

The stability, reliability and efficiency of a smart ship are important issues as the interest in an autonomous ship has recently been high. An automatic collision avoidance system is an essential function of an autonomous ship. This system detects the possibility of collision and automatically takes avoidance actions in consideration of economy and safety. In order to construct an automatic collision avoidance system using reinforcement learning, in this work, the sequential decision problem of ship collision is mathematically formulated through a Markov Decision Process (MDP). A reinforcement learning environment is constructed based on the ship maneuvering equations, and then the three key components (state, action, and reward) of MDP are defined. The state uses parameters of the relationship between own-ship and target-ship, the action is the vertical distance away from the target course, and the reward is defined as a function considering safety and economics. In order to solve the sequential decision problem, the Deep Deterministic Policy Gradient (DDPG) algorithm which can express continuous action space and search an optimal action policy is utilized. The collision avoidance system is then tested assuming the $90^{\circ}$intersection encounter situation and yields a satisfactory result.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.6
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• pp.1023-1037
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• 2018

The interest in the use of shield TBM (Tunnel Boring Machine) on the tunnel excavation has been increased rapidly in Korea. The shield TBM tunnel is generally designed as non-drainage tunnel. Consequently, if water leakage through the segment joints happens, big problems on the usage and stability of tunnel can be occurred. In this study, the variation of waterproof capacity of hydrophilic rubber waterstop and gasket, respectively by the construction error and excessive displacement of segment was studied. The test results show that hydrophilic rubber waterstop has favorable on the offset, however unfavorable on the gap. On the other hand, gasket has unfavorable on the offset, however favorable on the gap.

• Journal of the Korean GEO-environmental Society
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• v.20 no.10
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• pp.5-14
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• 2019

Recently, earthquakes have occurred in our country and seismic stability of high-rise buildings in large cities is being a growing interest and thus the related studies have been increased. Also the grounds are considered indirectly in most of seismic designs and analyses and seismic researches based on 3D dynamic analysis are insufficient. In this study, therefore, 2D and 3D dynamic analyses were performed based on the SSI complete model including grounds and the behavior was compared and analyzed. For dynamic modeling, linear time history analyses were performed by using MIDAS GTS NX. For this purpose, a high-rise building was assumed to be constructed on top of the bedrock and surrounded by a surface layer. A sensitivity analysis was performed with the selected parameters. The dynamic behavior was compared and analyzed in terms of horizontal displacements, drift ratios, bending stresses, and weak parts. In most cases, 2D dynamic behavior was calculated to be larger than 3D's and thus it shows more conservative results with increasing number and size of weak parts.

• Journal of Industrial Convergence
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• v.13 no.4
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• pp.11-28
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• 2015

A company have to grow constantly. If a company does not grow and stagnant, it will be finally out of the market. The contemporary companies fully make use of M&A to search for new growth engines. The reason of companies using M&A as a important tool of a business strategy is the fastest way to achieve technology power, market power, competitiveness. The form of M&A was that leading companies take over smaller companies or merger and acquisition between small companies in the middle of 2000. But now, Mega mergers between industry leading companies often occur and especially domestic of course M&A of foreign companies occurs actively. These days the boom of stock market and the big companies are pouring on sale by restructuring, privatization and the basis of low interest will make the M&A market continuously. In this study, I suggest a solution of actual human resources management by analyzing proven M&A cases and search for various problems of a gap in the leadership and communication in connection with integration of organization culture after M&A. First of all, I arrange the theoretical concept of the subject and analyze the key factors of the success M&A cases, lastly I suggested a HR strategy after M&A. After M&A, HR strategy is ; First, a company have to build a organization culture which is that merger company accommodate a excellent organizational characteristic of predecessor company with consideration of culture difference. Second, M&A must proceed to remove of anxiety about the future and employment stability by excellent leaderships. Third, organization integration after M&A is influenced by the level of integration for that reason it was verified that M&A have to make progress by communication of each 2 organizations.