• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Journal of KIISE:Software and Applications
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• v.33 no.1
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• pp.44-57
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• 2006

With the rapid growth of network technology, two or more products from different vendors are integrated and interact with each other to perform a certain function in the latest systems. Thus. interoperability testing is considered as an essential aspect of correctness of integrated systems. Interoperability testing is to test the ability of software and hardware on different machines from different vendors to share data. Most of the researches model communication system behavior using EFSM(Extended Finite State Machines) and use EFSM as an input of test scenario generation algorithm. Actually, there are many studies on systematic and optimal test case generation algorithms using EFSM. But in these researches, the study for generating EFSM model which is a foundation of test scenario generation isn't sufficient. This study proposes an EFSM generating technique from informal requirement analysis document for more complete interoperability testing. and implements prototype of Test Case Generation Tool generating test cases semi-automatically. Also we describe theoretical base and algorithms applied to prototype implementation.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.367-371
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• 2004

This paper describes development of automatic flight control system for an unmanned target drone which is operated by Korean army as for anti-air gun shooting training. Current target drone is operated by pilot control of on-board servo motor via remote control system. Automatic flight control system for the target drone greatly reduces work load of ground pilot and can increase application area of the drone. Most UAVs being operated now days use high-priced sensors as AHRS and IMU to measure the attitude, but those are costly. This paper introduces the development of low-cost automatic flight control system with low-cost sensors. The integrated automatic flight control system has been developed by integrating combining power module, switching module, monitoring module and RC receiver as an one module. The performance of automatic flight control system is verified by flight test.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.846-851
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• 1995

A motor-integrated high-speed spindle system with .psi. 65*25, 000rpm is modeled for analytical and experimental studies related to the dynamic characteristics. And the systematic and rational identification processes for evaluating the material properties of spindle and built-in motor is introduced. The impulse excitation method is applied for the experimental model testing, and the dynamic characteristics of test model is theoretically analysed by using the finite element method based on Timoshenko theory. Especially, the experimental and theoetical results reveal that the test model under the required operational conditions has no critical problem for dynamic characteristics.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.139-142
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• 2001

As microeletronics technology continues to progress, there is also a continuous demand on highly integration and miniaturization of systems. For example, it is desirable to package several integrated circuits together in multilayer structure, such as multichip modules, to achieve higher levels of compactness and higher performance. Passive components (i.e., capacitors, resistors, and inductors) are very important for many MCM applications. In addition, the low-temperature co-fired ceramic (LTCC) process has considerable potential for embedding passive components in a small area at a low cost. In this paper, we investigate a method of statistically modeling integrated passive devices from just a small number of test structures. A set of LTCC inductors is fabricated and their scattering parameters (5-parameters) are measured for a range of frequencies from 50MHz to 5GHz. An accurate model for each test structure is obtained by using a building block based modeling methodology and circuit parameter optimization using the HSPICE circuit simulator.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2003.05a
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• pp.171-176
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• 2003

A Test Facility was established to carry out the integrated propulsion performance tests(IPPT). To perform IPPT's with maximum safety, an emergency blockage system was investigated. An emergency blockage system using combustion chamber pressure and acceleration signals was set up to monitor ignition delay and fail, flame out, propellant feeding status, unstable combustion and excessive structural vibration. With such system, the maximum safety could be secured by rapid judgement and follow-up measures, which made IPPT's be safely completed.

• Proceedings of the KSR Conference
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• 2007.05a
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• pp.680-685
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• 2007

Power semiconductor which is adapted in the rolling stock has a high practicality for capacity of high voltage and high speed switching. but it has a trouble of fever cause of high speed On, Off switching loss and the operating junction temperature is limited to $150^{/circ}C$ because is made from the silicon for the foundation material. Therefore, it is important to find a way out of this trouble and must make the countermeasure. In this research, the caloric value of the integrated PEM is calculated to adapt the optimized heat pipe and the reliability of the heat pipe is demonstrated through the cooling performance test and vibration test.

• Proceedings of the Korea Society for Simulation Conference
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• 2004.05a
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• pp.101-108
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• 2004

KEPRI has developed an Nuclear Steam Supply System(NSSS) thermal-hydraulics simulation program (called ARTS-KORI), based on the best-estimate system code, RETRAN, as a part of the development project for the KORI unit 1 Nuclear Power Plant Simulator. A number of code modifications, such as simplifications and removing of discontinuities of the physical correlations, were made in order to change the RETRAN code as an nuclear Steam Supply System thermal-hydraulics engine in the simulator. Some simplified models and a backup system were also developed. This paper briefly presents the results of non-integrated standalone test of ARTS-KORI.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.10a
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• pp.117-122
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• 1995

An Integrated Test Facility(ITF) is a human factors experimental environment to evaluate an advanced man machine interface(MMI) design. The ITF includes a human machine simulator(HMS) comprised of a nuclear power plant function simulator, man-machine interface, experiment control station for the experiment control and design, human behavioural data measurement system, and data analysis and experiment evaluation supporting system(DAEXESS). The most important features of ITF is to secure the flexibility and expandibility of Man Machine Interlace(MMI) design to change easily the environment of experiments to accomplish the experiment's objects In this paper, we describe a development scope and characteristics of the ITF such as, hardware and software development scope and characteristics, system thermohydraulic modelling characteristics, and experiment station characteristics for the experiment variables design and control, to be used as an experiment environment for the evaluation of VDU-based control room.

• IE interfaces
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• v.4 no.2
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• pp.53-66
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• 1991

For the strategic management of the small and/or mid sized clinics, the introduction of the clinic information system is necessary. Currently there are not many information systems for the clinics, yet. In this study, we have developed a clinic information system (CLINSYS) as a guiding prototype and suggested the development directions of the total integrated clinic decision support system. For the better user interface and easy communication. the object-oriented language Macintosh Hyper-Card and Excel are used as the developing tools. And as a part of the integrated decision system, an expert system to consult about the urine test and the blood test interpretation has been developed using the Macintosh spreadsheet Excel.