• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2005년도 창립 30주년 및 춘계학술대회
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• pp.47-54
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• 2005

수퇘지에서는 타 종의 수컷에서와는 달리 성선호르몬이 자발적인 사료섭취를 억제하다. 따라서 거세 돼지는 수퇘지나 암퇘지에 비해 사료섭취량이 과다하여 과비해지는 경향이 있다. 본 연구에서는 교잡종 거세비육돈에서 Revalor H[실험 I; 140mg trenbklone acetate(합성 androgen) + 14mg estradiol-$17\beta(E_{2}\beta)$] 및 Compudose[실험 II; 24mg $E_{2}\beta$]를 implantation 했을 때 성장효율, 도체특성 및 혈중 IGH-I 및 IGF-binding protein-3(IGFBP-3) 농도에 미치는 영향을 조사하고자 2회의 사양실헝(I & II)를 수행하였다. 실험 I에서는 생체중 약60kg의 교잡종 거세비육돈 64두를 8돈방에 완전임의배치하여 2[implant; 처리 대비 무처리] $\times$ 2[사양; 무제한급여 대비 $80\%$ 무제한급여] $\times$ 2[사료; 대조(NRC 권장 수준의 $103\%$) 대비 저에너지($87\%$ NRC 권장량) 사료] 'factorial' 사양시험을 수행하였고, 실험 II에서는 80두의 거세비육돈(10두/돈방)을 이용하여 implant의 효과 유무를 조사하였다. 실험 I과 II에서 모든 공시돈은 생체중 $100\~110kg$ 시 도축되었다. Revalor H와 Compudose implant 제제는 공히 사료섭취량과 등지방두께를 저하시키고, 도체의 주요 이화학적 특성에는 영향을 미치지 않고 혈중 IGF-I 농도를 증가시켰다. 또한 이들 측정항목에서 Revalor H implant는 제한사양, 저에너지 사료, 혹은 Compudose 이상의 효과를 나타내었고, 증체를 억제하였으나 사료효율은 증진시켰으며, 후자(사양, 사료)와의 상호작용은 나타나지 않았다. 이상의 결과는 거세비육돈에서 1) androgen과 estrogen은 공히 자발적인 사료섭취와 등지방 침적을 억제하고 IGF-I 분비를 증가시키며, 2) 성선스테로이드호르몬의 이 같은 성장에 미치는 효과의 일부는 IGF-I을 통해 매개될 수도 있을을 시사한다.

• Clinical and Experimental Pediatrics
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• 제48권8호
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• pp.865-870
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• 2005

목 적 : 최근까지 성장호르몬 결핍증으로 진단 받은 소아에서의 성장호르몬 치료는 그 효과를 인정받아 왔으나 성장호르몬 자극 유발 검사에서 유의한 결과를 보이지 않는 특발성 저신장증은 그 원인이 성장호르몬의 부분적 결핍 또는 감수성의 부족 때문이므로 성장호르몬의 치료 효과가 기대되나 성장호르몬 투여가 인정되어 오지 못한 현실이다. 특발성 저신장 소아에서의 성장호르몬의 효과에 대하여 몇몇 국외 연구 논문들이 발표되어 왔으나 국내에서는 거의 없는 실정으로 이에 저자들은 국내 소아를 대상으로 국내에서 생산된 성장호르몬을 투여하기 전과 후의 여러 성장 관련인자들 및 신장을 비교 분석하여 특발성 저신장증에서의 성장호르몬 치료효과에 대하여 검증하고자 하였다. 방 법 : 충북대학교병원에서 최소 1년 이상 성장호르몬 투여를 받은 특발성 저신장증 환아 15명을 대상으로 하여 1년 치료군과 2년 치료군으로 나누어 투여 전 및 후의 여러 성장 관련변수들 및 신장의 변화를 검토하여 성장호르몬의 효과를 비교 분석하였다. 결 과 : 15명의 소아 중 남아가 7명, 여아가 8명이었고 평균 연령은 $11.44{\pm}2.81$세(4.6-12.73세)이었다. 성장호르몬을 사용하기 전에 1년 치료군과 2년 치료군 사이의 역 연령, 골 연령, 신장 표준 편차치, 예측 성인 신장의 표준 편차치, 표적 신장의 표준 편차치, IGF-I, 그리고 IGFBP-3의 차이는 없었다. 또한 성장호르몬 투여 1년 후의 각종 성장 관련변수들의 비교 결과 1년 치료군과 2년 치료군 사이의 유의한 차이는 없었다. 그러나 성장호르몬 투여 전후의 신장의 변화를 비교하였을 때 1년 치료군과 2년 치료군 모두에서 의미 있는 증가를 보였다. 또한 각종 성장 관련 변수들도 두 군 모두에서 증가를 보였으나 2년 치료군에서는 IGF-I과 IGFBP-3가 통계학적으로 관련성이 미미한 것처럼 보였는데 이는 환아의 수가 적기 때문으로 보인다. 결 론 : 통상적으로 최대 자극 성장호르몬 치를 성장호르몬 치료의 기준으로 사용하여 왔다. 그러나 이 연구에서는 성장호르몬 자극 검사상 성장호르몬 결핍증의 범위에서 벗어나는 특발성 저신장의 치료에도 성장호르몬이 효과가 있으며 또한 치료의 지표로서 IGF-I과 IGFBP-3를 사용할 수 있다는 것을 보여주고 있다.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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• pp.40-46
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• 2001

The use of pluripotent stem cells has tremendous advantages for various purposes but these cell lines with proven germ-line transmission have been completely established only in the mouse. Embryonic germ (EG) cell lines are also pluripotent and undifferentiated stem cells established from primordial germ cells (PGCs). This study was conducted to establish and characterize the chicken EG cells derived from gonadal primordial germ cells. We isolated gonadal PGCs from 5.5-day-old (stage 28) White leghorn (WL) embryos and established chicken EG cells lines with EG culture medium supplemented with human stem cell factor (hSCF), murine leukemia inhibitory factor (mLIF), bovine basic fibroblast growth factor (bFGF), human interleukin-11 (hIL-11), and human insulin-like growth factor-I (hIGF-I). These cells grew continuously for 4 months (10 passages) on a feeder layer of mitotically active chicken embryonic fibroblasts. These cells were characterized by screening with the Periodic acid-Shiff＇s reaction, anti-SSEA-1 antibody, and a proliferation assay after several passages. As the results, the chicken EG cells maintained characteristics of undifferentiated stem cells as well as that of gonadal PGCs. When cultured in suspension, the chicken EG cells successfully formed an embryoid body and differentiated into a variety of cell types when re-seeded onto culture dish. The chicken EG cells were injected into blastodermal layer at stage X and dorsal aorta of recipient embryo at stage 14 (incubation of 53hrs) and produced chimeric chickens with various differentiated tissues derived from the EG cells. The germline chimeras were also successfully induced by using EG cells. Thus, Chicken EG cells will be useful for the production of transgenic chickena and for studies of germ cell differentiation and genomic imprinting.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1090-1094
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• 2006

Histone acetylation modification is one key mechanism in the regulation of gene activation. In this study, we investigated the global levels of histone H4 acetylation of insulin like growth factor I (IGF1) and growth hormone (GH) genes in the lungs of two somatic cell cloned calves. Data showed the levels of histone H4 acetylation of IGF1 and GH genes vary widely within different gene regions, and, in almost all regions of the two genes, acetylation levels are lower in the aberrant clone than in the normal clone. Thus we suggest that inefficient epigenetic reprogramming in the clone may affect the balance between acetylation and deacetylation, which will affect normal growth and development. These findings will also have implications for improvement of cloning success rates.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.716-722
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• 2005

Carcass weight and backfat thickness are primary yield grading factors. Insulin-like growth factor (IGF)-I/-II, transforming growth factor $\beta$1 (TGF-$\beta$1), and epidermal growth factor (EGF) regulate the proliferation and differentiation of cells including adipocytes. Also, interleukin (IL)-2/-6, cortisol, and dehydroepiandrosterone-sulfate (DHEA-S) are known to be related to muscle growth and fat depth. However, the relationships between endocrine factors and carcass grade have not been studied. Therefore, this study aimed to measure the concentrations of endocrine factors in serum and muscle, and to investigate the relationship of endocrine factors with carcass grade. A total of 60 crossbred gilts (Duroc${\times}$Yorkshire${\times}$Landrace) were used. Blood from the jugular vein was collected at antemortem (7 days before slaughter) and postmortem periods, and M. Longissimus was collected at 45 min and 24 h after slaughter. The concentrations of IGF-I/-II, EGF, TGF-$\beta$1, IL-2/-6, cortisol and DHEA-S were analyzed by radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA). In general, IGF and EGF concentrations in serum and muscle of grade A carcasses were found to be higher than those of grade C carcasses at antemortem and postmortem periods, whereas the pattern of TGF-$\beta$1 concentration was reversed. In particular, the concentrations of muscle IGF-I (24 h postmortem) and serum TGF-$\beta$1 (antemortem) were significantly different between grades A and C (p<0.05). The present results indicate that serum and muscle growth factors affect carcass weight and backfat thickness, and indirectly suggest the possibility that carcass grade could be predicted by expression of serum and/or muscle growth factors.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.595-606
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• 2018

Objective: The Fusarium mycotoxins of deoxynivalenol (DON) and zerolenone (ZEN) cause health hazards for both humans and farm animals. Therefore, the main intention of this study was to reveal DON and ZEN effects on the mRNA expression of pro-inflammatory cytokines and other immune related genes in the liver of piglets. Methods: In the present study, 15 six-week-old piglets were randomly assigned to the following three different dietary treatments for 4 weeks: control diet, diet containing 8 mg DON/kg feed, and diet containing 0.8 mg ZEN/kg feed. After 4 weeks, liver samples were collected and sequenced using RNA-Seq to investigate the effects of the mycotoxins on genes and gene networks associated with the immune systems of the piglets. Results: Our analysis identified a total of 249 differentially expressed genes (DEGs), which included 99 upregulated and 150 downregulated genes in both the DON and ZEN dietary treatment groups. After biological pathway analysis, the DEGs were determined to be significantly enriched in gene ontology terms associated with many biological pathways, including immune response and cellular and metabolic processes. Consistent with inflammatory stimulation due to the mycotoxin-contaminated diet, the following Kyoto encyclopedia of genes and genomes pathways, which were related to disease and immune responses, were found to be enriched in the DEGs: allograft rejection pathway, cell adhesion molecules, graft-versus-host disease, autoimmune thyroid disease (AITD), type I diabetes mellitus, human T-cell leukemia lymphoma virus infection, and viral carcinogenesis. Genome-wide expression analysis revealed that DON and ZEN treatments downregulated the expression of the majority of the DEGs that were associated with inflammatory cytokines (interleukin 10 receptor, beta, chemokine [C-X-C motif] ligand 9), proliferation (insulin-like growth factor 1, major facilitator superfamily domain containing 2A, insulin-like growth factor binding protein 2, lipase G, and salt inducible kinase 1), and other immune response networks (paired immunoglobulin-like type 2 receptor beta, Src-like-adaptor-1 [SLA1], SLA3, SLA5, SLA7, claudin 4, nicotinamide N-methyltransferase, thyrotropin-releasing hormone degrading enzyme, ubiquitin D, histone $H_2B$ type 1, and serum amyloid A). Conclusion: In summary, our results demonstrated that high concentrations DON and ZEN disrupt immune-related processes in the liver.

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.439-446
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• 2005

This study was conducted to elucidate the influence of recombinant bovine somatotropin (rBST) on the plasma IGF-I profiles in deer stags. Slow release rBST (250mg each) preparations were injected subcutaneously during the antler's non-growing period and growing period. It was observed that significant increases in the plasma IGF-I concentrations were occurred right after injections of the rBST irrespective of antler's non-growing period or growing period. When the rBST preparations were injected two times with one week interval during the antler's non-growing period, the mean level of IGF-I was found to be 2,920.7ng I mL, indicating that the IGF-I concentration increased up to 8.4-folds compared with a control. After two weeks from second shot, however, the IGF-I levels decreased to the level of the control. Similar results were also obtained with both a Sika and a Red deer during the antler's growing period. Increases in the IGF-I level of up to 5.6-folds with the Sika deer and 4.3-folds with the Red deer were found. Based on the present study, it is likely that the rBST may be used as a possible candidate for an antler's growth stimulating agent.

• Journal of Nutrition and Health
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• 제39권3호
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• pp.225-235
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• 2006

Although it has traditionally known that deer antler and medicinal herbs extract contain some functional components for health promotion, the nutritional significance remains to be elucidated. This study examined the efficacy of deer antler extract (DA) , medicinal herbs extract (MH) and their mixture (DAMH) on serum IGF-I, bone growth with growing rats in vivo and splenocyte proliferation with spleen cells in vitro. Three week-old young female rats (Sprague-Dawley) were divided into 4 groups and then fed basal diet (AIN-93G) or experimental diets containing DA, MH, DAMH, respectively, for 7 weeks. We collected blood, liver, kidney, spleen, femur and tibia from rats. There was no significant difference in weight gain, but food intake increased in DA- and MH-fed groups. There were no signs of liver and kidney damage in the DA, MH and DAMH-fed groups compared to basal diet group. In femur and tibia, wet weights: breaking forces and bone minerals (Ca, Mg and Zn) were significantly higher in the DA-fed group than in the other groups. Serum alkaline phosphatase (ALP) , bone-specific alkaline phosphatase (BALP) activities were significantly lower in the DA, MH, DAMH-fed groups than in basal diet group. Also, serum insulin-like growth factor-I (IGF-I) concentrations were significantly increased in DA-fed group compared to the other groups. Therefore DA was shown to have an activity of bone growth promotion by increasing the IGF-I, a major bone growth factor. The deer antler extract showed an enhanced immune action on the primary cultured-cells from spleen of rats, representing that splenocytes were proliferated by lipopolysaccharide (LPS), but not by concanavalin A (Con A). These results indicate that deer antler extract has beneficial effects on bone growth via IGF-I and on splenocyte activation.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.72-72
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• 2003

대부분의 동물에서 체내 또는 체외수정란의 체외 배양 시 일정한 발육 단계까지 발달한 후 발육지연이나 정지가 되는 체외 발육억제 현상이 나타나게 된다. 특히 돼지에서는 타 가축들과는 달리 4 세포기에서 체외 발육억제 현상이 일어나기 때문에 체외에서의 발육율이 매우 낮아 수정란 생산이 제한되고 있다. 따라서 본 연구는 이러한 돼지 체외발육 억제 현상을 극복하고 돼지 체외 수정란의 체외배양 체계의 기초 자료를 얻고자 돼지 미성숙 난자를 체외에서 성숙, 수정시킨 뒤, 체외 수정란의 배양 시 성장인자와 6탄당의 첨가에 따른 체외 발육율을 검토하였다. 난자의 핵 성숙과 세포질 성숙 및 세포 기능에 영향을 미치는 것으로 알려져 있는 성장인자로는 Insulin-like growth factor-I(IGF-I)과 Epidermal growth factor(EGF)를 사용하였고, 여러 종의 번식기관에 존재하여 배반포 형성을 촉진시키는 것으로 알려진 glucose, mannose, galactose 및 fructose가 6탄당으로 사용되었다. 체외수정란의 발육을 위한 기본 배양액인 NCSU-23에 각각 0, 1, 5, 10 및 20ng/ml의 IGF-I과 EGF를 각각 첨가하여 농도의 차이에 따른 발육율을 검토하였다. 또한 5.56mM의 glucose, mannose, galactose 및 fructose에 5ng/ml의 IGF-I 또는 10ng/ml의 EGF 첨가 유, 무에 따른 초기배 발육율을 검토하였다. 마지막으로, 각각의 6탄당에 위와 같은 농도의 IGF-I와 EGF 공동 첨가 유, 무에 따른 초기배 발육율을 검토하였다. 그 결과 돼지 체외 수정란의 체외 발육 시 배양액 내에 서로 다른 농도의 IGF-I과 EGF를 첨가하였을 때 IGF-I은 5ng/ml(12%)에서, EGF는 10ng/ml(10%)의 실험구에서 가장 높은 배반포기 배의 발육율을 나타냈다. 또한 각각의 6탄당과 IGF-I 또는 EGF 유, 무에 따른 초기배 발육율을 검토한 결과 IGF-I과 EGF 모두 glucose 첨가 시 타 첨가구에 비해 초기 발육 단계의 수정란 발육뿐만 아니라 배반포까지의 배발육(10～11%)이 타 첨가구(3～8%)에 비해 높게 나타났다. 한편, 각각의 6탄당이 첨가된 배양액 내에 IGF-I파 EGF 공동첨가 유, 무에 따른 초기배 발육율을 검토한 결과 모든 실험구에서 EGF와 IGF-I 첨가 시 무첨가보다 높은 초기 배 발육율을 나타냈으며 특히 초기 분열단계 수정란에서는 발육의 차이가 크게 나타났다. 본 연구 결과 성장인자와 6탄당의 첨가는 돼지 수정란의 체외배양 시 초기배 배발육에 효과적인 영향을 미치는 것으로 사료되며, 이는 체외 발육율이 타 가축에 비해 낮은 돼지의 수정란 생산에 있어 체외배양체계의 개선을 위한 기초자료가 될 수 있을 것이라 기대된다.

• 대한수의학회지
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• 제40권4호
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• pp.755-762
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• 2000

The aim of this investigation was to examine the effects of osteocalcin, bone-specific alkaline phophatase, estrogen, insulin-like growth factor-I (IGF-I), Ca, P and bone mineral density on osteoporosis induced by ovariectomy in rats. Female Sprague-Dawley 30 rats of three-forth's birth, weighing $215{\pm}10g$, were divided into two groups including the sham operation group(5 heads) and ovariectomy group(25 heads). They were fed normal diets for 2 weeks before the experimental operation and for 8 more weeks after operation. The level of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were increased in experimental group, but a little increased in sham operation group at same period. The change of rates of osteocalcin, TALP, BALP, estrogen, bone mineral density and IGF-I were significantly higher in experimental group than sham operation group. $Ca^{2+}$ was not changed between two groups and P was significantly decreased in experimental group and Ca/P ratio was higher in experimental group than sham operation group. Body weights were increased in all two groups and growth rate per day was higher in experimental group than sham operation group. However, femur weight I body weight ratio was lower in experimental group than sham operation group.