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http://dx.doi.org/10.5713/ajas.2005.716

Expression of Serum and Muscle Endocrine Factors at Antemortem and Postmortem Periods and Their Relationship with Pig Carcass Grade  

Kim, W.K. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Kim, M.H. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Ryu, Y.H. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Ryu, Y.C. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Rhee, M.S. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Seo, D.S. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Lee, C.Y. (Regional Animal Industry Research Center)
Kim, B.C. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Ko, Y. (Department of Animal Science, College of Life and Environmental Sciences, Korea University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.18, no.5, 2005 , pp. 716-722 More about this Journal
Abstract
Carcass weight and backfat thickness are primary yield grading factors. Insulin-like growth factor (IGF)-I/-II, transforming growth factor $\beta$1 (TGF-$\beta$1), and epidermal growth factor (EGF) regulate the proliferation and differentiation of cells including adipocytes. Also, interleukin (IL)-2/-6, cortisol, and dehydroepiandrosterone-sulfate (DHEA-S) are known to be related to muscle growth and fat depth. However, the relationships between endocrine factors and carcass grade have not been studied. Therefore, this study aimed to measure the concentrations of endocrine factors in serum and muscle, and to investigate the relationship of endocrine factors with carcass grade. A total of 60 crossbred gilts (Duroc${\times}$Yorkshire${\times}$Landrace) were used. Blood from the jugular vein was collected at antemortem (7 days before slaughter) and postmortem periods, and M. Longissimus was collected at 45 min and 24 h after slaughter. The concentrations of IGF-I/-II, EGF, TGF-$\beta$1, IL-2/-6, cortisol and DHEA-S were analyzed by radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA). In general, IGF and EGF concentrations in serum and muscle of grade A carcasses were found to be higher than those of grade C carcasses at antemortem and postmortem periods, whereas the pattern of TGF-$\beta$1 concentration was reversed. In particular, the concentrations of muscle IGF-I (24 h postmortem) and serum TGF-$\beta$1 (antemortem) were significantly different between grades A and C (p<0.05). The present results indicate that serum and muscle growth factors affect carcass weight and backfat thickness, and indirectly suggest the possibility that carcass grade could be predicted by expression of serum and/or muscle growth factors.
Keywords
Endocrine Factor; rowth Factor; Carcass Grade; Pig;
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1 Barton-Davis, E. R., D. I. Shoturma, A. Musaro, N. Rosenthal and H. L. Sweeney. 1998. Viral mediated expression of insulin-like growth factor I blocks the aging-related loss of skeletal muscle function. Proc. Natl. Acad. Sci. USA. 95:15603-15607.
2 Boudarene, M., J. J. Legros and M. Timsit-Berthier. 2002. Study of the stress response: role of anxiety, cortisol and DHEAs. Encephale (Paris). 28:139-146.
3 Clarke, L., D. S. Buss, D. T. Juniper, M. A. Lomax and M. E. Symonds. 1997. Adipose tissue development during early postnatal life in ewe-reared lambs. Exp. Physiol. 82:1015-1027.
4 Ignotz, R. A. and J. Massague. 1985. Type beta transforming growth factor controls the adipogenic differentiation of 3T3 fibroblasts. Proc. Natl. Acad. USA. 82: 8530-8534.
5 Jones, J. I. and D. R. Clemmons. 1995. Insulin-like growth fators and their binding proteins: biological actions. Endocr. Rev. 16:3-34.
6 Khouw, I. M., P. B. van Wachem, J. A. Plantinga, Z. Vajaskovic, M. J. Wissink, L. F. de Leij and M. J. van Luyn. 1999. TGF-beta and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro. Biomaterials 20: 1815-1822.
7 Lamberson, W. R., J. A. Sterle and R. L. Matteri. 1996. Relationship of serum insulin-like growth factor II concentrations to growth, compositional and reproductive traits of swine. J. Anim. Sci. 74:1753-1756.
8 Lea-Currie, Y. R., P. Wen and M. K. McIntosh. 1998. Dehydroepiandrosterone reduces proliferation and differentiation of 3T3-L1 preadipocytes. Biochem. Biophys. Res. Commun. 248:497-504.
9 McNamara, J. P. and J. E. Pettigrew. 2002. Protein and fat utilization in lactating sows: I. Effects on milk production and body composition. J. Anim. Sci. 80:2442-2451.
10 Pampusch, M. S., J. R. Hembree, M. R. Hathaway and W. R. Dayton. 1990. Effect of transforming growth factor beta on proliferation of L6 and embryonic porcine myogenic cells. J. Cell Physiol. 143:524-528.
11 Pampusch, M. S., E. Kamanga-Sollo, M. E. White, M. R. Hathaway and W. R. Dayton. 2003. Effect of recombinant porcine IGF-binding protein-3 on proliferation of embryonic porcine myogenic cell cultures in the presence and absence of IGF-I. J. Endocrinol. 176:227-235.   DOI   ScienceOn
12 Richardson, R. L., G. J. Hausman and H. R. Gaskins. 1992. Effect of transforming growth factor-beta on insulin-like growth factor 1 and dexamethasone-induced proliferation and differentiation in primary cultures of pig preadipocytes. Acta. Anat (Basel). 145:321-326.
13 Rotwein, P. 1991. Structure, evolution, expression and regulation of insulin-like growth factors I and II. Growth Factors 5:3-18.
14 Sutton, D. S., M. Ellis, Y. Lan, F. K. McKeith and E. R. Wilson. 1997. Influence of slaughter weight and stress gene genotype on the water-holding capacity and protein gel characteristics of three porcine muscles. Meat Sci. 46:173-180.
15 Wise, T., J. Klindt and F. C. Buonomo. 1995. Obesity and dehydroepiandrosterone/dehydroepiandrosterone sulfate relationships in lean, obese, and meat-type cross-bred boars: responses to porcine growth hormone. Endocrinology 136:3310-3317.
16 Yoon, J., M. S. Rhee, D. S. Seo, B. C. Kim and Y. Ko. 2001. Monitoring of blood cytokines by PIT-1 genotype in day 150 male pigs. Asian-Aust. J. Anim. Sci. 14:1659-1664.
17 Burr, J. G., G. Dreyfuss, S. Penman and J. M. Buchanan. 1980. Association of the Src gene product of Rous sarcoma virus with cytoskeletal structures of chicken embryo fibroblasts. Proc. Natl. Acad. Sci. USA. 77:3484-3488.
18 Ma, X., M. Labinaz, J. Goldstein, H. Miller, W. J. Keon, M. Letarte and E. O'Brien. 2000. Endoglin is overexpressed after aterial injury and is required for transforming growth factorbeta-induced inhibition of smooth muscle cell migration. Arterioscler. Thromb. Vasc. Biol. 20:2546-2552.
19 Peng, M., M. F. Palin, S. Veronneau, D. LeBel and G. Pelletier. 1997. Ontogeny of epidermal growth factor (EGF), EGF receptor (EGFR) and basic fibroblast growth factor (bFGF) mRNA levels in pancreas, liver, kidney and skeletal muscle of pig. Domest. Anim. Endocrinol. 14:286-94.   DOI   ScienceOn
20 Clore, J. N. 1995. Dehydroepiandrosterone and body fat. Obes. Res. 3:613S-616S.   DOI
21 Sarbassov, D. D., R. Stefanova, V. G. Grigoriev and C. A. Peterson. 1995. Role of insulin-like growth factors and myogenin in the altered program of proliferation and differentiation in the NFB4 mutant muscle cell line. Proc. Natl. Acad. Sci. USA. 92:10874-10878.
22 Ebisu, C., T. Tsujinaka, T. Morimoto, K. Kan, S. Iijima, M. Yano, E. Konimani, K. Tanaka and M. Monden. 1995. Interleukin-6 induces proeolysis by activating intracellular proteases (cathepsin B and L, proteasome) in C2C12. Clin. Sci. 89:431-439.
23 Brun, J. F. 2002. Hormones, metabolism and body composition as major determinants of blood rheology: potential pathophysiological meaning. Clin. Hemorheol. Microcirc. 26:63-79.
24 Buonomo, F. C. and J. Klindt. 1993. Ontogeny of growth hormone (GH), insulin-like growth factors (IGF-I and IGF-II) and IGF binding protein-2 (IGFBP-2) in genetically lean and obese swine. Domest. Anim. Endocrinol. 10:257-265.
25 Choy, L. and R. Derynck. 2003. Transforming growth factor-beta inhibits adipocyte differentiation by Smad3 interacting with CCAAT/enhancer-binding protein (C/EBP) and repressing C/EBP transactivation function. J. Biol. Chem. 278:9609-9619.
26 Dwyer, C. M. and N. C. Stickland. 1992. The effects of maternal undernutrition on maternal and fetal serum insulin-like growth factors, thyroid hormones and cortisol in the guinea pigs. J. Dev. Physiol. 18:303-313.
27 Ryu, H., H. S. Lee, Y. S. Shin, S. M. Chung, M. S. Lee, H. M. Kim and H. T. Chung. 1996. Acute effect of qigong training on stress hormonal levels in man. Am. J. Chin. Med. 24:193-198.
28 Sparks, R. L., B. J. Allen and E. E. Strauss. 1992. TGF-beta blocks early but not late differentiation-specific gene expression and morphologic differentiation of 3T3T proadipocytes. J. Cell Physiol. 150:568-567.
29 Adachi, H., H. Kurachi, H. Homma, K. Adachi, T. Imai, M. Sakata, Y. Matsuzawa and A. Miyake. 1995. Involvement of epidermal growth factor in inducing adiposity of age female mice. Endocrinology 146:381-393.
30 Abraham, E. 1991. Effects of stress on cytokine production. Methods Achiev. Exp. Pathol. 14:45-62.
31 Kanaoka, Y., T. Yagi, H. Sadamori, H. Matsukawa, H. Matsuda, M. Inagaki, T. Ishikawa, S. Saito, H. Iwagaki and N. Tanaka. 2002. Analysis of host response to hepatectomy by simultaneous measurement of cytokines in the portal vein, caval vein and radial artery. J. Int. Med. Res. 30:496-505.
32 Goodman, M. N. 1994. Interleukin-6 induces skeletal muscle protein breakdown in rats. Proc. Soc. Exp. Biol. Med. 205:182-185.   DOI
33 Kunesova, M., V. Hainer, J. Obenberger, R. Mikulova, J. Parizkova, S. Slaba, D. Bezdickova and Z. Seidl. 2002. Adipose tissue distribution in obese females: relationship to androgens, cortisol, growth hormone and leptin. Sb. Lek. 103:477-485.
34 Yun, J. S., D. S. Seo, M. S. Rhee, S. Oh, B. C. Kim and Y. Ko. 2003a. Relationships of concnetrations of endocrine Factors at antemortem and postmortem periods to carcass weight and backfat Thickness in pigs. Asian-Aust. J. Anim. Sci. 16:335-341.
35 Bony, C. M., B. M. Moats-Staats, A. D. Stiles and A. J. D’Ercole. 1994. Expression of insulin-like growth factor-I (IGF-I) and IGF-binding proteins during adipogenesis. Endocrinology 135:1863-1868.
36 Rahimi, N., E. Tremblay, L. McAdam, A. Roberts and B. Elliott. 1998. Autocrine secretion of TGF-beta 1 and TGF-beta 2 by pre-adipocytes and adipocytes: a potent negative regulator of adipocyte differentiation and proliferation of mammary carcinoma cells. In vitro Cell Dev. Biol. Anim. 34:412-420.
37 O’Quinn, P. R., B. S. Andrews, R. D. Goodband, J. A. Unruh, J. L. Nelssen, J. C. Woodworth, M. D. Tokach and K. Q. Owen. 2000. Effects of modified tall oil and creatine monohydrate on growth performance, carcass characteristics, and meat quality of growing-finishing pigs. J. Anim. Sci. 78:2098-2103.
38 Sterin-Borda, L., C. PerezLeiros, E. S. Borda and M. M. Bracco. 1996. Effect of IL-2 on the myocardium. Participation of the sympathetic system. J. Mol. Cell Cardiol. 28:2457-2465.
39 Yun, J. S., W. J. Kang, D. S. Seo, C. Y. Lee, S. Oh and Y. Ko. 2003b. Relationships of circulating of insuline-like growth factor (IGF)-I and –II to egg production and growth rate in the Korean Native Ogol Chicken. Asian-Aust. J. Anim. Sci. 16:481-488.
40 Butterwith, S. C. 1997. Regulators of adipocyte precursor cells. Poult. Sci. 76:118-123.   DOI
41 Lee, C. Y. and D. M. Henricks. 1990. Comparisons of various acidic treatments of bovine serum on insuli-like growth factor-I immunoreactivity and binding activity. J. Endocrinol. 127:139-148.
42 Marques, B. G., D. B. Hausman, A. M. Latimer, K. M. Kras, B. M. Grossman and R. Y. Martin. 2000. Insulin-like growth factor I mediates high-fat diet-induced adipogenesis in Osbornmendetl rat. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278:654-662.
43 te Pas, M. F., A. Soumillion, F. L. Harders, F. J. Verburg, T. J. van den Bosch, P. Galesloot and T. H. Meuwissen. 1999. Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness and lean weight of pigs. J. Anim. Sci. 77:2352-2356.
44 Underwood, L. E., A. J. D'Ercole, D. R. Clemmons and J. J. Van Wyk. 1986. Paracrine functions of somatomedins. Clin. Endocrinol. Metab.15:59-77.
45 Berk, B. C. 2001. Vascular smooth muscle growth: autocrine growth mechanisms. Physiol. Rev. 81:999-1030.   DOI
46 Schoknecht, P. A., S. Ebner, A. Skottner, D. G. Burrin, T. A. Davis, K. Ellis and W. G. Pond. 1997. Exogenous insulin-like growth factor-I increases weight gain in intrauterine growth-retarded neonatal pigs. Pediatr. Res. 42:201-207.
47 Fraser, R., M. C. Ingram, N. H. Anderson, C. Morrison, E. Davies and J. M. Connell. 1999. Cortisol effects on body mass, blood pressure, and cholesterol in the general population. Hypertension 33:1364-1368.
48 Parker, L. N., E. R. Levin and E. T. Lifrak. 1985. Evidence for adrenocortical adaptation to severe illness. J. Clin. Endocrinol. Metab. 60:947-952.
49 Yun, J. S., W. J. Kang, D. S. Seo, S. S. Park, K. C. Hong, C. Y. Lee and Y. Ko. 2001. Association of endocrine factors (insulin-like growth factor-II and binding protein-3) with litter size in pigs. Asian-Aust. J. Anim. Sci. 14:307-315.
50 Cowherd, R. M., R. E. Lyle and R. E. Jr. McGehee. 1999. Molecular regulation of adipocyte differentiation. Semin. Cell Dev. Biol. 10:3-10.
51 Szabo, C., A. J. Jansman, L. Babinszky, E. Kanis and M. W. Verstegen. 2001. Effect of dietary protein source and lysine:DE ratio in growth performance, meat quality, and body composition of growing-finishing pigs. J. Anim. Sci. 79:2857- 2865.
52 Corino, C., G. Oriani, L. Pantaleo, G. Pastorelli and G. Salvatori. 1999. Influence of dietary vitamin E supplementation on 'heavy' pig carcass characteristics, meat quality, and vitamin E status. J. Anim. Sci. 77:1755-1761.
53 Bass, J., J. Oldham, M. Sharma and P. Kambadur. 1999. Growth factors controlling muscle development. Domest. Anim. Endocrinology 17:191-197.
54 Florini, J. R., D. Z. Ewton and S. A. Coolican. 1996. Growth hormone and the insulin-like growth factor system in myogenesis. Endocr. Rev. 17:481-517.
55 Shaw, F. D. and G. R. Trout. 1995. Plasma and muscle cortisol measurement as indicators of meat quality and stress in pigs. Meat Sci. 39:237-246.
56 Kurach, H., H. Adachi, S. Ohtsuka, K. Morishige, K. Amemiya, Y. Keno, I. Shimomura K. Tokunaga and Y. Matsuzwa. 1993. Involvement of incducing obesity in ovariectomize mice. Am. J. Physiol. 265 (2 pt 1):E323-E331.
57 Roemmich, J. N. and A. D. Rogol. 1999. Hormonal changes during puberty and their relationship to fat distribution. Am. J. Human. Biol. 11:209-224.