• Title/Summary/Keyword: Instrumental analyses

검색결과 145건 처리시간 0.025초

기기적 평가와 패널 기반 평가를 이용한 된장 용액의 제시 온도에 따른 맛과 향미 특성 분석 및 비교 (A Comparison of Flavor and Taste of the Doenjang Solution by Instrumental Measurements and Sensory Evaluation based on Serving Temperature)

  • 허정애;곽한섭;김미정;김민정;김윤숙;장민선;김상숙
    • 한국식품영양학회지
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    • 제31권2호
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    • pp.264-271
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    • 2018
  • The objective of this study was to investigate taste profiles and the correlation of Doenjang soup solution prepared with three different temperatures (5, 25, $45^{\circ}C$) and using an electronic nose (e-nose), electronic tongue (e-tongue), and descriptive analyses by trained panelists. A total of 17sensory attributes were generated from the descriptive analyses for the Doenjang soup solution. There were significant difference among the samples in the cooked soybean flavor, the cooked soybean odor, the soy sauce odor, the sweet odor, the sweet taste, and the sweet aftertaste attributes. The intensities of these sensory attributes tended to increase as the serving temperature of the Doenjang soup solution increased. There were seven volatile compounds detected by the electronic nose: ethanol, propanal, 2-methylpropanal, ethyl acetate, 3-methylbutanal, and beta-pinene. The intensities of the volatile compounds increased as the temperature of the Doenjang soup solution increased. On the other hand, the intensities of the basic tastes by the e-tongue decreased as the temperature of the Doenjang soup solution increased. In conclusion, the e-nose, e-tongue, and descriptive analysis results showed different correlations depending on the temperature of the Doenjang soup solutions.

냉면 육수 조립법의 표준화 연구 (Development of a Standardized Recipe for Korean Cold Noodle Stock)

  • 김업식;최일숙;구성자
    • 한국식품조리과학회지
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    • 제17권6호
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    • pp.589-597
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    • 2001
  • This study was conducted to develop a standardized Korean cold noodle stock recipe which can be used in food service establishments. The qualities of three kinds of stock made of beef rib only(B group), beef rib added with chicken(BC group), and beef rib added with chicken and vegetable(BCV group) were investigated by using sensory evaluation and instrumental analyses for free amino acids and nucleotides during heating (2, 3, 4, and 6 hr) at 90$\pm$ 5$\^{C}$ The highest amino acid contained in B, BCV, and BCV groups was arginine followed by alanine, glycine, and glutamic acid. B and BC groups was extracted to the best contents of amino acids by heating for 4 hours but BCV group for 3 hours. Nucleotides were extracted from B and BC group between 3 and 4 hours of heating but in BCV group between 2 and 3 hours. In sensory evaluation, BCV group obtained the highest score for overall preference. In the measurement of color difference, BCV group was the lowest in L value(lightness) but the highest in b value(yellowness).

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영유아교사 인성교육지도역량 척도 개발 (The Development of the Teaching Competency Scale for Character Education in Early Childhood Teachers)

  • 고은경;전효정
    • 아동학회지
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    • 제37권5호
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    • pp.129-144
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    • 2016
  • Objective: This study's purpose was to develop a teaching competency scale for character education for early childhood teachers and to examine its reliability. Methods: The study procedure was as follows: First, scale items were generated by reviewing the literature as a deductive approach and focused interviews with 8 field experts in an inductive approach. Second, face validation was assessed by 7 academic experts, and statistical validation processes were done twice. The data used in the final statistical analyses included 206 questionnaires for early childhood teachers. Statistical validation included item response analysis, item discrimination analysis, exploratory factor analysis, confirmatory factor analysis, and reliability analysis. Results: The 3 factors identified were as follows: Communicate teaching competence, practical teaching competence, and instrumental teaching competence. The validity and reliability of the Teaching Competency Scale for Character Education in Early Childhood Teachers were acceptable. Conclusion: This new scale will be a useful tool to better support character education for early childhood teachers and contribute to providing a basis for developing more sophisticated tools.

Verlamelin, an Antifungal Compound Produced by a Mycoparasite, Acremonium strictum

  • Kim, Jin-Cheol;Park, Gyung-Ja;Kim, Hyun-Ju;Kim, Heung-Tae;Ahn, Jong-Woong;Cho, Kwang-Yun
    • The Plant Pathology Journal
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    • 제18권2호
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    • pp.102-105
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    • 2002
  • A strain of Acremonium strictum, the mycoparasite of Botrytis cinerea, showed strong antifungal activities both in vitro and in vivo against several phytopathogenic fungi. An antifungal substance was purified from the liquid cultures of A. strictum and identified as verlamelin by instrumental analyses. Verlamelin exhibited in vitro antifungal activity against some phytopathogenic fungi such as Magnaporthe grisea, Bipolaris maydis, and Botrytis cinerea, while it was net active against all the bacteria tested. In viva, verlamelin exhibited strong protective and curative activities, particularly against barley powdery mildew. At 100 μg/ml, it inhibited the development of barley powdery mildew with control values of more than 90% in 7-day protective and 2-day curative applications. This is the first report on the production of verlamelin by Acremonium species.

Canister와 GC/MSD를 이용한 대기 중 VOCs 분석시스템의 정도관리(QC) (Quality Control(QC) Analysis of Ambient VOCs in Canisters Using GC/MSD Method)

  • 전준민;허당;김동술
    • 한국대기환경학회지
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    • 제18권6호
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    • pp.527-538
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    • 2002
  • In this study, the performance of sampling and of analytical methodology was examined against a number of selected volatile organic compounds (VOCs) in the ambient air. The canister-based sampling method for VOCs has been a viable and widely used approach that is based on research and evaluation performed over the past several years. VOCs were determined using canisters by GCMSD analysis. A total of target analyses in this study included approximately 30 VOCs designated in TO-l4A categories including BTEX. The methodology for QC(quality control) was intensively investigated with a wide range of performance criteria such as; (1) cleanliness of canisters, (2) reproducibility of diluter, (3) instrumental detection limits of preconcentrator and GCMSD, and (4) precision and accuracy of GCMSD. For most of the compounds tested, precision was less than $\pm$5%, IDL was 0.2 ppbv, accuracy was $\pm$5%, respectively. This study demonstrated that the combination of canister sampling and GC/MSD analysis could be reliably applied to the measurements of BTEX in ppbv levels that are typically observed in/outdoor air environment.

Streptomyces endus YP-1이 생산하는 항암활성 물질의 분리 및 정제

  • 최성원;김병찬;최선진;김동섭;여익현;문순옥;오두환
    • 한국미생물·생명공학회지
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    • 제25권5호
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    • pp.483-489
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    • 1997
  • Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RNA dot blot and Northern hybridization analysis were performed to screen microorganisms for the production of anticancer agent. Among microorganisms tested, strain YP-1 was selected for its cytotoxicity and ability to reduce the level of c-myc RNA. Strain YP-1 was identified as Streptomyces endus. The anticancer material produced by Streptomyces endus YP-1 was sequentially purified by solvent extraction, silica gel column chromatograpby, preparative TLC and preparative HPLC. The cancer material identified as azalomycin B by the instrumental analyses such as $^{1}$H-NMR, $^{13}$C-NMR, Mass, IR and UV absorption. It was colorless amorphous powder and its molecular weight was 1025.278. Azalomycin B, produced by Streptomyces endus YP-1, showed anticancer activity against several human cancer cell lines and reduction of c-Myc protein level in Colo320 DM cells which was determined by Western blot analysis.

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식물병원균 Bipolaris sorokiniana로부터 제초활성 물질의 분리 및 구조결정 (Isolation and Structure Determination of Phytotoxins from a Phytopathogenic Fungus Bipolaris sorokiniana)

  • 임치환
    • 농업과학연구
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    • 제29권2호
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    • pp.91-97
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    • 2002
  • 식물병원균 Bipolaris sorokiniana 배양 추출물로부터 이탈리안 라이그라스 및 논피의 유근신장을 억제하는 활성물질을 분리 정제하여 각종 기기분석을 통하여 화학구조를 결정하였다. 이들 화합물들은 분자식이 $C_8H_8O_3$인 benzenoid 화합물인 3-methoxybenzoic acid와 3-hydroxy benzoic acid methyl ester로 숙주식물의 유근신장을 1000ppm에서 90% 이상 저해하였다. 이들 화합물은 식물체의 잎과 줄기에서 분리되어 항균활성 및 항류마티스 활성이 있음이 보고된바 있으나 B. sorokiniana로부터 분리되어 제초활성에 관하여 보고하기는 본 논문이 처음이다.

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Exploring Reasoning Patterns of Students' Scientific Thinking, Inquiry Activities in Textbook, and Examination Items

  • Kim, Young-Shin;Kwon, Yong-Ju;Yang, Il-Ho;Chung, Wan-Ho
    • 한국과학교육학회지
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    • 제23권4호
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    • pp.309-318
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    • 2003
  • Scientific reasoning is one of the main concerns in current science education. This study have tried to answer on the question whether Korean science education has the potential to help improve of students' ability to think scientifically. Therefore, the present study investigated the relationship between reasoning patterns evident in science textbook and science examination items, and students' scientific reasoning skills across grades in Korea. 1975 subjects (1022 females and 953 males) were administered in the Lawson's Test of Scientific Reasoning skills. Forty seven science textbooks and 240 assessment instrument were analyzed by several scientific reasoning keys. Scientific reasoning patterns were adopted from Lawson's classification which characterized the patterns as the empirical-inductive and the hypothetical-deductive. This study found that reasoning patterns evident in textbook analyses and assessment instrumental items do not evidentce the potential to stimulate the development of students' reasoning skill. In order to improve the students' abilities to think and achieve, higher levels of reasoning must be included in the science textbook and examination. Further, some of scientific reasoning processes, such as generating hypotheses, designing experiments, and logical prediction, were not found in science textbooks and test items in Korean secondary schools. This study also discussed the educational implication of these results and further studies about to develop student's reasoning ability.

산사 분획의 혈관이완 활성과 성분분석에 관한 연구 (Vasodilatory Effect of the Fractions from Crataegus pinnatifida and Isolation of the Active Component)

  • 최호정;신동훈;김길훤;신흥묵
    • 동의생리병리학회지
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    • 제17권5호
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    • pp.1235-1242
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    • 2003
  • This study was performed for the investigation of vasodilatory effects of Crataegus pinnatifida Bunge and for isolation and structure determination of the constituent from the active fraction. The fruits of this herbal drug were extracted with 80% methanol, then fractioned successively with methylene chloride, ethylacetate and n-butanol. Among the fractions, ethyl acetate fraction exhibited the most effective vascular relaxation against phenylephrine-induced arterial contraction. In order to isolate the active constituent by activity-guided fractionation, this fraction was chromatographed on silica gel to yield seven subfractions. Among the subfractions, the active one showing the most potent vascular relaxation activity was further separated by prep. HPLC with reversed phase Microsorb C-18 column using 1 % acetic acid and methanol gradient solvent system to afford one pure compound, which revealed a potent vasodilatory effect. Instrumental analyses (NMR and mass spectrometry) of the isolated constituent indicated this compound to be (-)-epicatechin. The vasodilatory action mechanism of this compound should be further investigated.

노인간병가족의 부담요인 및 부담완화를 위한 사회복지서비스와 실천방법의 효과에 관한 연구 (Factors of Caregiving Burden and the Effectiveness of Social Welfare Services for the Family Caregivers of the Impaired Older Persons)

  • 이인정
    • 한국사회복지학
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    • 제46권
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    • pp.319-348
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    • 2001
  • The primary purpose of this study is to examine the buffering effect of social welfare services on the relationship between the level of older persons' impairment and burden experienced by the family caregivers. It also assessed the level of caregiving burden as well as the effect of both older person's impairment and the characteristics of the caregivers on burden. The survey data collected from 150 community residing family caregivers was used for analyses. Findings are as follows. First, the caregivers are more burdened in the deterioration of mental health, scarce time for oneself, and the suffering of social life than in other areas of burden. Second, the more severe the physical and the mental impairment of the elder, the more burden the caregivers experience. The caregivers' characteristics such as the worse mental health, the lower level of attachment to the elder, the longer caregiving hours are also related to the higher level of caregiving burden. Third, the caregivers' use of adult day care and respite care services buffer the relationship between the level of impairment of older persons and the caregiving burden. However, social work counselling, visiting nurse, homemaker services do not have such buffering effects. Among informal support, instrumental support buffers the relationship between the elder's physical impairment and burden, while emotional support buffers the relationship between the elder's mental impairment and burden. According to the results, implication for social welfare services and practice methods for the family caregivers was discussed.

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