Browse > Article

Vasodilatory Effect of the Fractions from Crataegus pinnatifida and Isolation of the Active Component  

Choi Ho Jeong (Department of Physiology, College of Oriental Medicine, Dongguk University)
Shin Dong Hoon (Department of Physiology, College of Oriental Medicine, Dongguk University)
Kim Gil Whon (Department of Physiology, College of Oriental Medicine, Dongguk University)
Shin Heung Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.5, 2003 , pp. 1235-1242 More about this Journal
Abstract
This study was performed for the investigation of vasodilatory effects of Crataegus pinnatifida Bunge and for isolation and structure determination of the constituent from the active fraction. The fruits of this herbal drug were extracted with 80% methanol, then fractioned successively with methylene chloride, ethylacetate and n-butanol. Among the fractions, ethyl acetate fraction exhibited the most effective vascular relaxation against phenylephrine-induced arterial contraction. In order to isolate the active constituent by activity-guided fractionation, this fraction was chromatographed on silica gel to yield seven subfractions. Among the subfractions, the active one showing the most potent vascular relaxation activity was further separated by prep. HPLC with reversed phase Microsorb C-18 column using 1 % acetic acid and methanol gradient solvent system to afford one pure compound, which revealed a potent vasodilatory effect. Instrumental analyses (NMR and mass spectrometry) of the isolated constituent indicated this compound to be (-)-epicatechin. The vasodilatory action mechanism of this compound should be further investigated.
Keywords
Crataegi pinnatifida Bung; vascular relaxation; HPLC; (-)-epicatechin;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 The treatment f mild stble forms of angina pectoris using Ctataegutt novo /
[ Hanack, T.;Bruckel, M.H. ] / Therapiewoche
2 산사山楂의 혈관이완 효능과 항산화작용 /
[ 손창우;채종구;김길훤;신흥묵 ] / 동의생리병리학회지
3 내피세포 Nitric Oxide 유리를 통한 山 의 혈관이완 작용 /
[ 채종구;김길훤;신흥묵 ] / 동의생리병리학회지
4 산사 Butanol 분획이 PGF2a-유도 혈관평활근 수축의 억제에 미치는 신호전달 연구 /
[ 유가량;최호정;김길훤;신흥묵 ] / 동의생리병리학회지
5 Effect of Butanol Fraction of Crataegi Fructus on the translocation of PKCa and myosin phosphotase subnits and LC20 phosphorylation in vascular smooth muscle /
[ 이헌제 ] / 동국대학교 대학원 한의학과 석사학위논문
6 Crataegus, toxicology and pharmacology. II. Pharmacodynamics /
[ Ammon, H. P. T.;Handel, M. ] / Plant medica
7 Crataegus, toxicology and pharmacology. III. Pharmacodynamics and Pharmacokinetics /
[ Ammon, H. P. T.;Handel, M. ] / Plant medica
8 Effect of hawthorn human vascular endothelial cell /
[ Chang, C.;Chen, J. ] / Yingyang Xuebao
9 Identification of phenolic antioxidative coponents in crataegus pinnatifida bunge /
[ Kim, J.S.;Lee, G.D.;Kwon, J.H.;Yoon, H.S. ] / Han'guk Nonghwa Hakhoechi
10 Characterization of antioxidants present in hawthorn fruits /
[ Zhang, Z.;Chang, Q.;Zhu, M.;huang, Y.;Walter, K.K. Ho;Chen, Z.Y. ] / Journal of Nutritional Biochemistry   ScienceOn
11 Analysis of the chemical constituents of hawthorn fruits and their quality evaluation /
[ Cao, G.Y.;Feng, Y.X.;Qin, X.Q. ] / Acta. Pharm. Sin.
12 Dierary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study /
[ Hertog, M.G.L.;Feskens, E.J.M., Hollman, P.C.H., Katan, M.B., Kromhout, D. ] / Lancet   ScienceOn
13 Cardoactive composition comprising L-carnitine and its derivatives and craataegus extracts /
[ Cavazza, C. ] / PCt int. Appl.
14 /
[ 康秉秀;金永坂 ] / 臨床配合本草
15 /
[ 李時珍 ] / 本草綱目
16 Hawthorn/Passionflower extract and impro- vement in physical capacity of patients with dyspnoea Class II of the NYHM functional classification /
[ M. von Eiff. ] / Acta Ther.
17 Crataegus, toxicology and pharmacology. I. /
[ Ammon, H. P. T.;Hande,l M. ] / Toxicity, Plant medica
18 Therapeutic effectiveness of crataegus /
[ Weihmayr, T.;Emst, E. ] / Forschritte der medizin
19 Myocadial effects of flavonoids from crataegus species /
[ Schussler, M.;Holzl, J.;Fricke, U. ] / Arzneim-Forsch Drug Res.